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Anti-GLIPR1 Antibody Ab118382

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Anti-GLIPR1 Antibody Ab118382 Product Datasheet Anti-GLIPR1 antibody ab118382 3 Images Overview Product name Anti-GLIPR1 antibody Description Mouse monoclonal to GLIPR1 Tested applications WB, ELISA, Flow Cyt Species reactivity Reacts with: Human Immunogen Recombinant fragment, corresponding to amino acids 23-100 of Human GLIPR1 with proprietary tag (NP_006842). Positive control HeLa whole cell lysate. Properties Form Liquid Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Storage buffer pH: 7.20 Constituent: 99% PBS Purity Protein A purified Clonality Monoclonal Isotype IgG2a Light chain type kappa Research Areas Cell Biology Apoptosis Intracellular p53 Pathway Cancer Oncoproteins/suppressors Tumor suppressors p53 pathway Cancer Invasion/microenvironment Apoptosis Other Applications Our Abpromise guarantee covers the use of ab118382 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Notes WB WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 30 kDa. ELISA ELISA: Use at an assay dependent concentration. Flow Cyt Flow Cyt: Use 0.1µg for 106 cells. Target Tissue specificity According to PubMed:8973356, it is ubiquitously expressed with high levels in lung and kidney and low levels in heart and liver. Highly expressed in cell lines derived from nervous system tumors arising from glia, low or absent in non-glial-derived nervous system tumor cell lines. Also found in fetal kidney. According to PubMed:7607567 it is expressed only in brain tumor glioblastoma multiforme/astrocytoma and not in other nervous system tumors or normal fetal or adult tissues. Sequence similarities Belongs to the CRISP family. Cellular localization Membrane. Target information above from: UniProt accession P48060 http://www.abcam.com/GLIPR1-antibody-ab118382.html 1 Product Datasheet The UniProt Consortium The Universal Protein Resource (UniProt) in 2010 Nucleic Acids Res. 38:D142-D148 (2010) . Database links Entrez Gene: 11010 Human Omim: 602692 Human SwissProt: P48060 Human Unigene: 205558 Human Alternative names CRISP 7 antibody CRISP7 antibody GLI pathogenesis related 1 antibody GLI pathogenesis-related 1 (glioma) antibody Glioma pathogenesis related protein 1 antibody Glioma pathogenesis-related protein 1 antibody GLIP1_HUMAN antibody GliPR 1 antibody GLIPR antibody GLIPR1 antibody Protein RTVP-1 antibody RELATED TO TESTIS SPECIFIC, VESPID, AND PATHOGENESIS PROTEINS 1 antibody RTVP 1 antibody RTVP1 antibody Testes specific vespid and pathogenesis protein 1 antibody testes-specific vespid and pathogenesis protein 1 antibody Anti-GLIPR1 antibody images Overlay histogram showing HepG2 cells stained with ab118382 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118382, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Flow Cytometry - Anti-GLIPR1 antibody Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab118382) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. http://www.abcam.com/GLIPR1-antibody-ab118382.html 2 Product Datasheet Anti-GLIPR1 antibody (ab118382) at 5 µg/ml + HeLa cell lysate at 50 µg Predicted band size : 30 kDa Western blot - Anti-GLIPR1 antibody (ab118382) Anti-GLIPR1 antibody (ab118382) at 5 µg/ml + recombinant immunogen at 0.2 µg/ml Predicted band size : 30 kDa Western blot against tagged recombinant immunogen using ab118382 antibody at 5µg/ml dilution. Predicted band size of immunogen is 34.58kDa. Western blot - Anti-GLIPR1 antibody (ab118382) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support As standard Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery** Response to your inquiry within 24 hours In addition We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors **Regional variations to our Abpromise may apply to the following countries: China, Korea, Singapore, Malaysia, Taiwan and Thailand, which operate a 120 day guarantee. Please contact your regional office for further details Antibodies tested only in recombinant samples are not covered for use on endogenous samples. Please check the datasheet for information on tested species "Predicted to react" information on datasheets is based on sequence homology and provided for reference only "Predicted to react" species are not guaranteed, as they have not been tested http://www.abcam.com/GLIPR1-antibody-ab118382.html 3 Product Datasheet Fast Track products are not covered under the Abpromise. Please see Fast Track terms and condition for additional information Abcam biochemicals are novel compounds and we have not tested their biological activity in house. Please use the literature to identify how to use these products effectively. If you require further assistance please contact the scientific support team Cambridge Office Abcam plc 330 Cambridge Science Park Cambridge CB4 0FL UK Tel: +44 (0)1223 696000 Fax: +44 (0)1223 215 215 Email: [email protected] Boston Office Abcam Inc. 1 Kendall Square, Suite B2304 Cambridge, MA 02139-1517 USA Tel: (888) 77-ABCAM (22226) Fax: (877) 774-8286 These numbers are toll free in the US/Canada Email: [email protected] Visit us at: www.abcam.com http://www.abcam.com/GLIPR1-antibody-ab118382.html 4.
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