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Lab Standard Operating Procedures (Sops) QAPP –Watershed Watch Laboratory Assays Revision: 7 Date: September 2020 Appendix A Standard Operation Procedures List of SOPs SOP SOP Revision Description Number Date General Laboratory Safety 001 11/04 1 URI Laboratory Waste Guidebook 001a 09/13 3 URI laboratory Chemical Hygiene Plan 001b 07/04 Laboratory Water 002 11/16 3 General Labware Cleaning Procedure 003 07/19 4 General Autoclave Operation 004 10/18 6 Bottle Autoclaving Procedure 005 01/16 5 Waste Autoclaving Procedure 006 01/16 3 Chlorophyll-A Analysis, Welschmeyer Method 012 04/18 6 Chloride Analysis 013 11/16 5 Ammonia Analysis 014 11/16 5 Orthophosphate and Nitrate + Nitrite Analysis 015 12/16 5 Total Phosphorus and Nitrogen Analysis 016 12/16 5 Salinity Analysis Using a Refractometer 017 11/16 1 Enterococci Analysis Using Enterolert IDEXX Method 018 12/19 4 Analytical Balance Calibration 019 2/06 2 pH Procedure 021 12/19 3 Alkalinity Procedure 022 11/16 2 Filtering Water Samples 023 10/18 2 Fecal coliform Analysis Using Colilert 18 IDEXX 024 01/16 2 Method Laboratory Thermometer Calibration 025 04/19 1 Heterotrophic Plate Count – Quanti-tray 026 05/20 1 Appendix A Standard Operating Procedure 001 Date: 11/04 General Laboratory Safety Revision: 1 Author: Linda Green University of Rhode Island Watershed Watch 1.0 PURPOSE AND DESCRIPTION LAB SAFETY IS EVERYBODY’S JOB! Please be sure to familiarize yourself with these general procedures, as well as the specific handling requirements included in the Standard Operating Procedure (SOP) for each analysis/process. Further general information regarding University of Rhode Island standards for health and safety are found in SOP 001a – University Safety and Waste Handling Document. 2.0 HEALTH AND SAFETY 2.1 Emergency Numbers: EMERGENCIES: 874-2121 UNCONTAINED SPILLS: 874- 2618 GENERAL HEALTH AND SAFETY INFORMATION URI SAFETY AND RISK: 874-2618 2.2 General Health and Safety Information 1. Eye protection, gloves and lab coats are REQUIRED in this laboratory when working with chemicals. 2. Closed-toed shoes are REQUIRED whenever you are working in the lab. Even if you are just entering data. 3. Know where the accident and safety equipment is: First–aid kit: i. Behind the sink in room 018. ii. On top of the refrigerator in room 002. iii. On top of the refrigerator in room 019. Safety shower: next to the entry door of each laboratory. Eye wash: at main sink in each laboratory. Yellow Spill pads: i. Under the laboratory bench to the right of blue oven in room 018. ii. Next to the main sink in room 002. iii. On top of the refrigerator in room 019 General Laboratory Safety SOP 001 1 of 2 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPPRev5-0609\SOPs\SOP 001 Safety-mod.doc 4. Report ANY accidents IMMEDIATELY to Linda Green (874-2905) or Elizabeth Herron (874- 4552). If neither are available, first contact staff in the Cooperative Extension Water Quality Suite (room 001), Dr. Art Gold (874-2903) or department secretary (874-2026). Report all emergencies to 874-2121. 5. If you spill anything hazardous on yourself immediately flush it with water for 15 minutes. Report all emergencies to 874-2121. Uncontained spills should be reported to 874-2618. 6. Concentrated acids and bases are ALWAYS handled in a fume hood, with the glass door pulled down below face level. 7. Wastes are ALWAYS stored in the lab in which they are generated (i.e. acetone waste in the fluorometer lab, autoanalyzer waste in the instrument lab, etc.). Do not transport wastes from one lab to another. All wastes must have a University of Rhode Island (URI) Safety and Health label on them. 8. Liquid wastes must have secondary containment adequate for the full volume of waste. 9. When waste containers are nearing full (~ 80% for high volume wastes or 90% for low volume wastes), coordinate with Linda Green or Elizabeth Herron to have them removed. Be sure all waste containers are properly and completely labeled at all times. 10. As appropriate, use a cart or a bucket with a handle to transport water samples and/or reagents. 11. All chemicals must be properly labeled and stored at all times. Hazardous labels must indicate in English what harm the chemical represents (ie corrosive). 12. Liquid chemicals can not be stored above eye level. 13. If you have any safety or environmental questions, call URI Safety and Risk at 874-2618. 14. All URI Watershed Watch (WW) staff including student technicians are required to successfully complete URI’s “Environmental Awareness and Initial Waste Training” course within their first 6 months of employment. Subsequently, all URIWW staff including student technicians are required to successfully complete URI’s annual refresher course: “Prudent Practices and Laboratory Waste Management”. Certificates attesting to successful completion are posted in URIWW laboratories. General Laboratory Safety SOP 001 2 of 2 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPPRev5-0609\SOPs\SOP 001 Safety-mod.doc University of Rhode Island Laboratory Waste Guidebook September 2013 Website: https://web.uri.edu/ehs/hazardous_waste/ University of Rhode Island Laboratory Waste Guidebook Revised September 2013 Department of Public Safety Environmental Health and Safety 9/17/13 University of Rhode Island Laboratory Waste Guidebook September 2013 Environmental Policy & Principles In accordance with the University Safety Policy, the Department of Public Safety Environmental Health and Safety Office has established this Environmental Policy and these Principles. All members of the University are responsible for complying with this policy. Environmental Policy The University of Rhode Island is committed to compliance with all applicable federal, state and local regulations, to pollution prevention objectives, and to continual improvement of environmental systems. Environmental Principles To achieve this goal the University will adhere to the following Environmental Principles: • Comply with all applicable environmental laws and regulations. • Educate and train faculty, staff and students to properly implement University programs and procedures. • Minimize University impact on the environment and surrounding community. • Continually reduce University impact to the environment by implementation of pollution prevention and waste minimization programs. • Monitor adherence with University programs by measurement of performance against established goals and matrices. 9/17/13 University of Rhode Island Laboratory Waste Guidebook September 2013 Table of Contents Resources and Emergency Contact Information............................................................................. 1 1. Laboratory Waste Management ................................................................................................. 2 1.1 Enforcement ......................................................................................................................... 2 2. Laboratory Safety....................................................................................................................... 3 2.1 Personal Protective Equipment ............................................................................................ 3 2.2 Proper Laboratory Apparel .................................................................................................. 3 3. Chemical Waste Management ................................................................................................... 3 4. Hazardous Chemical Waste ....................................................................................................... 4 4.1 Waste Characterization Checklist ........................................................................................ 4 4.2 Hazardous Waste Containers ............................................................................................... 5 4.3 Incompatible Materials ........................................................................................................ 6 4.4 Hazardous Waste Labels ...................................................................................................... 6 5. Laboratory Hazardous Waste Satellite Accumulation Area (SAA) .......................................... 7 6. Pick-Up and Disposal ................................................................................................................ 7 6.1 To Have Waste Removed From The Laboratory ................................................................. 8 6.2 Chemical Clean-Out............................................................................................................. 8 7. Specific Handling Procedures .................................................................................................... 8 7.1 HPLC Wastes ....................................................................................................................... 8 7.2 Mercury Waste ..................................................................................................................... 8 7.3 Photographic Wastes ............................................................................................................ 9 7.4 Radioactive Waste ............................................................................................................... 9 7.5 Other .................................................................................................................................... 9 7.6 Unknowns ............................................................................................................................ 9 7.7 Gas Cylinders ......................................................................................................................
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