ITPKA Polyclonal Antibody Inositol Polyphosphates That Are Important in Cellular Signaling

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ITPKA polyclonal antibody inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein Catalog Number: PAB4051 phosphorylation mechanisms control its activity. It is also a substrate for the cyclic AMP-dependent protein kinase, Regulatory Status: For research use only (RUO) calcium/calmodulin- dependent protein kinase II, and protein kinase C in vitro. ITPKA and ITPKB are 68% Product Description: Rabbit polyclonal antibody raised identical in the C-terminus region. [provided by RefSeq] against synthetic peptide of ITPKA. References: Immunogen: A synthetic peptide (conjugated with KLH) 1. Structure of a human inositol 1,4,5-trisphosphate corresponding to N-terminus of human ITPKA. 3-kinase: substrate binding reveals why it is not a phosphoinositide 3-kinase. Gonzalez B, Schell MJ, Host: Rabbit Letcher AJ, Veprintsev DB, Irvine RF, Williams RL. Mol Cell. 2004 Sep 10;15(5):689-701. Reactivity: Human 2. Simulations of inositol phosphate metabolism and its Applications: ELISA, WB-Ce interaction with InsP(3)-mediated calcium release. (See our web site product page for detailed applications Mishra J, Bhalla US. Biophys J. 2002 information) Sep;83(3):1298-316. 3. Inositol 1,4,5-trisphosphate 3-kinase A associates with Protocols: See our web site at F-actin and dendritic spines via its N terminus. Schell http://www.abnova.com/support/protocols.asp or product MJ, Erneux C, Irvine RF. J Biol Chem. 2001 Oct page for detailed protocols 5;276(40):37537-46. Epub 2001 Jul 23. Form: Liquid Purification: Protein G purification Recommend Usage: ELISA (1:1000) Western Blot (1:100-500) The optimal working dilution should be determined by the end user. Storage Buffer: In PBS (0.09% sodium azide) Storage Instruction: Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. Entrez GeneID: 3706 Gene Symbol: ITPKA Gene Alias: IP3KA Gene Summary: Regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of the inositol 1,4,5-trisphosphate 3-kinase is responsible for regulating the levels of a large number of Page 1/1 Powered by TCPDF (www.tcpdf.org).

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Investigation of the Underlying Hub Genes and Molexular Pathogensis in Gastric Cancer by Integrated Bioinformatic Analyses
bioRxiv preprint doi: https://doi.org/10.1101/2020.12.20.423656; this version posted December 22, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Investigation of the underlying hub genes and molexular pathogensis in gastric cancer by integrated bioinformatic analyses Basavaraj Vastrad1, Chanabasayya Vastrad*2 1. Department of Biochemistry, Basaveshwar College of Pharmacy, Gadag, Karnataka 582103, India. 2. Biostatistics and Bioinformatics, Chanabasava Nilaya, Bharthinagar, Dharwad 580001, Karanataka, India. * Chanabasayya Vastrad [email protected] Ph: +919480073398 Chanabasava Nilaya, Bharthinagar, Dharwad 580001 , Karanataka, India bioRxiv preprint doi: https://doi.org/10.1101/2020.12.20.423656; this version posted December 22, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract The high mortality rate of gastric cancer (GC) is in part due to the absence of initial disclosure of its biomarkers. The recognition of important genes associated in GC is therefore recommended to advance clinical prognosis, diagnosis and and treatment outcomes. The current investigation used the microarray dataset GSE113255 RNA seq data from the Gene Expression Omnibus database to diagnose differentially expressed genes (DEGs). Pathway and gene ontology enrichment analyses were performed, and a proteinprotein interaction network, modules, target genes - miRNA regulatory network and target genes - TF regulatory network were constructed and analyzed. Finally, validation of hub genes was performed. The 1008 DEGs identified consisted of 505 up regulated genes and 503 down regulated genes.
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ITPKA Antibody (C-Term) Purified Rabbit Polyclonal Antibody (Pab) Catalog # Ap8166b
苏州工业园区双圩路9号1幢邮编： 215000 电话： 0512-88856768 ITPKA Antibody (C-term) Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP8166b Specification ITPKA Antibody (C-term) - Product info Application WB Primary Accession P23677 Other Accession NP_002211 Reactivity Human, Mouse Host Rabbit Clonality Polyclonal Isotype Rabbit Ig Clone Names RB5359 Calculated MW 51009 ITPKA Antibody (C-term) - Additional info Gene ID 3706 Other Names Inositol-trisphosphate 3-kinase A, Inositol 1, 5-trisphosphate 3-kinase A, IP3 3-kinase A, IP3K A, InsP 3-kinase A, ITPKA All lanes : Anti-ITPKA Antibody (C-term) Target/Specificity at 1:1000 dilution Lane 1: human brain This ITPKA antibody is generated from rabbits immunized with lysate Lane 2: K562 whole cell lysate a KLH conjugated synthetic peptide between 345-375 amino Lysates/proteins at 20 µg per lane. acids from the C-terminal region of human ITPKA. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 Dilution dilution. Predicted band size : 51 kDa WB~~1:1000 Blocking/Dilution buffer: 5% NFDM/TBST. Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Storage Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. Precautions ITPKA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. ITPKA Antibody (C-term) - Protein Information Western blot analysis of hITPKA-E360 (Cat.# AP8166b) in K562 cell line and mouse lung tissue lysates (35ug/lane).
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