DOCSLIB.ORG

ABSTRACT ZENG, HAINIAN. Development

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
ABSTRACT ZENG, HAINIAN. Development ABSTRACT ZENG, HAINIAN. Development-dependent Formation and Metabolism of Anthocyanins and Proanthocyanidins in Acer Species. (Under the direction of David Danehower, William Hoffmann, Jenny Xiang and De-yu Xie). Anthocyanins are one of the richest pigments, which belong to flavonoid compounds in plant kingdom. They have many biological and ecological functions. Over the past many years, numerous efforts have been made to determine the biosynthetic pathway of anthocyanins and also to identify several regulatory proteins mainly in flowers and fruits of model plants and crop plants. However, many questions concerning the metabolism of anthocyanins in foliage remains unsolved. One example is “How can developmental processes impact on accumulation patterns of anthocyanins in leaves”. In this study, we choose several cultivars from one of the most popular ornamental plants Acer palmatum Thunb. to understand the mechanism of developmental changes of pigmentation in leaf. Several other maple species were also analyzed. We propose that the metabolism of anthocyanins play an essential role in such changes. We use an integrated approach of phytochemistry and metabolic profiling to determine the biosynthesis and metabolism of anthocyanins and their impacts on foliage color. Proanthocyanidin analysis was carried out as well to determine their relationship to both anthocyanin production and foliar coloration. We have found that even for green leaves with no/trace amount of detectable anthocyanins, the biosynthetic pathway of anthocyanidin/proanthocyanidin is still activated. Our results indicate that metabolic channeling directing the anthocyanidin pathway to the proanthocyanidin biosynthesis plays a very important role in pigmentation pattern change along developmental processes. Development-dependent Formation and Metabolism of Anthocyanins and Proanthocyanidins in Acer Species by Hainian Zeng A thesis submitted to the Graduate Faculty of North Carolina State University in partial fulfillment of the requirements for the degree of Master of Science Plant Biology Raleigh, North Carolina 2009 APPROVED BY: _______________________________ ______________________________ Deyu Xie Jenny Xiang Committee Chair ________________________________ ______________________________ William Hoffmann David Danehower DEDICATION This thesis is dedicated to my dearest grandma, Yinchun Zhang, who passed away recently. ii BIOGRAPHY The first day I set my foot off the Airbus and onto the land of America, I knew it would be a life change. However, the thing I didn’t realize back then, is that this change has been so dramatic since that first day of air sickness and jet lag. From a metropolis to a countryside city. From Shanghai, my beloved hometown for more than 20 years, to, Raleigh, the capital city of North Carolina, one of the biggest agricultural states in the United States. This is big. Even one year later, sometimes I would still be wondering about where I was and why I was here. From time to time I was startled to see some many local people around and couldn’t help but mumbling to myself: I was in America. How time flies! Now it’s been three years, such doubt doesn’t go away but become stronger. In those three years, ups and downs are the main melody. Sometime I felt determined and I could see the bright future just ahead of me. Sometimes I felt lost and lonely, alienated myself from the outside due to serious depression. Sometimes I got sick and feared about the medical bill showing hundreds of dollars balance. Sometimes I demanded myself to be a man when facing my grandma’s past. Yes, this is life, but is it? At last, I find out what I want the most. To be with my family and friends. To fight against injustice and evil. To live a life whenever and wherever I can be myself, whenever and wherever I am in charge of my own life. At least, I won’t regret it. This is the whole point of being alive, isn’t it? Not to regret! Thanks to these three years’ life experience, I have re-founded my goal and grasped the hang of how to lead a life. To be the king of my own “land”! iii ACKNOWLEDGEMENTS I want to give my sincere appreciation and to my major advisor, Dr. Deyu Xie. He has been a wonderful mentor and advisor for these three years. Additionally, he takes care of his graduate students like his own children and treats us with respect as friends. To me, it’s not overrated to say that he is a father-like idol. The only regret I can’t let it go is that I haven’t live up to Dr. Xie’s expectations mainly due to my own weaknesses and mistakes. I’d like to give my deep gratitude to my other committee members, Drs. Bill Hoffmann, Jenny Xiang and David Danehower. They kindly gave me a lot of instructions and suggestions on my project, as well as their generous help with my life. I can still recall the time that Dr. Hoffmann gave me a ride back to my dorm, and Thanksgiving holidays I was spending in Dr. Xiang’s house as well as the knowledge of phytochemistry passed on to me by Dr. Danehower. Here I want to show my genuine gratefulness to all of my advisors for their understanding, forgiveness and noble gestures. I may be far away from being a good graduate student, but they have been continuously working with me as great advisors. And again, I’d like to apologize for my immature behaviors from time to time. I hope I can obtain your forgiveness some day. Besides my advisors, I’d like to thank our lab manager, Dr. Lili Zhou for her help with my research and her care about me. Dr. Zhou is a “mother” to us and I wish she can be with her family soon and lead a happy life ever after. Of course I would like to thank my labmates, Mingzhu, Fatima and Patrick. Without their inspiring ideas, contribution to my research and encouragements, I wouldn’t have gone this far. And last but not least, salutes to my colleagues in PBGSA for their unsinkable friendship as well. iv TABLE OF CONTENTS LIST OF TABLES ........................................................................................................................ xi LIST OF FIGURES .................................................................................................................... xii CHAPTER 1 – LITERATURE REVIEW .................................................................................. 1 1.1 Plant flavonoids .................................................................................................................... 3 Introduction .............................................................................................................................. 3 Biosynthesis of flavonoids ........................................................................................................ 5 Ecological and physiological significances ............................................................................. 8 Nutraceutical significances .................................................................................................... 10 Taxonomic significances ........................................................................................................ 10 1.2 Anthocyanins and anthocyanidins .................................................................................... 12 Introduction ............................................................................................................................ 12 Types of anthocyanins and anthocyanidins ............................................................................ 13 Properties of anthocyanins ..................................................................................................... 15 Biosynthesis of anthocyanins .................................................................................................. 17 Ecological significances ......................................................................................................... 19 Nutraceutical significances .................................................................................................... 24 1.3 Proanthocyanidins .............................................................................................................. 25 Introduction ............................................................................................................................ 25 Types of proanthocyanidins................................................................................................ 25 Biosynthesis of proanthocyanidins ......................................................................................... 29 Ecological significances ......................................................................................................... 30 Nutraceutical significances .................................................................................................... 31 v CHAPTER 2 - FOLIAR ANTHOCYANINS AND PROANTHOCYANIDINS IN SIX ORNAMENTAL VARIETIES OF ACER PALMATUM ....................................................... 32 2.1 Introduction ........................................................................................................................ 32 2.2 Materials and methods ....................................................................................................... 39 2.2.1 Standard chemicals ............................................................................................................ 39 2.2.2 Plant materials ................................................................................................................... 39 2.2.3 Leaf collection ..................................................................................................................
Load more

Recommended publications
  • Chemistry and Pharmacology of Kinkéliba (Combretum
    CHEMISTRY AND PHARMACOLOGY OF KINKÉLIBA (COMBRETUM MICRANTHUM), A WEST AFRICAN MEDICINAL PLANT By CARA RENAE WELCH A Dissertation submitted to the Graduate School-New Brunswick Rutgers, The State University of New Jersey in partial fulfillment of the requirements for the degree of Doctor of Philosophy Graduate Program in Medicinal Chemistry written under the direction of Dr. James E. Simon and approved by ______________________________ ______________________________ ______________________________ ______________________________ New Brunswick, New Jersey January, 2010 ABSTRACT OF THE DISSERTATION Chemistry and Pharmacology of Kinkéliba (Combretum micranthum), a West African Medicinal Plant by CARA RENAE WELCH Dissertation Director: James E. Simon Kinkéliba (Combretum micranthum, Fam. Combretaceae) is an undomesticated shrub species of western Africa and is one of the most popular traditional bush teas of Senegal. The herbal beverage is traditionally used for weight loss, digestion, as a diuretic and mild antibiotic, and to relieve pain. The fresh leaves are used to treat malarial fever. Leaf extracts, the most biologically active plant tissue relative to stem, bark and roots, were screened for antioxidant capacity, measuring the removal of a radical by UV/VIS spectrophotometry, anti-inflammatory activity, measuring inducible nitric oxide synthase (iNOS) in RAW 264.7 macrophage cells, and glucose-lowering activity, measuring phosphoenolpyruvate carboxykinase (PEPCK) mRNA expression in an H4IIE rat hepatoma cell line. Radical oxygen scavenging activity, or antioxidant capacity, was utilized for initially directing the fractionation; highlighted subfractions and isolated compounds were subsequently tested for anti-inflammatory and glucose-lowering activities. The ethyl acetate and n-butanol fractions of the crude leaf extract were fractionated leading to the isolation and identification of a number of polyphenolic ii compounds.
    [Show full text]
  • Flavonoid Glucodiversification with Engineered Sucrose-Active Enzymes Yannick Malbert
    Flavonoid glucodiversification with engineered sucrose-active enzymes Yannick Malbert To cite this version: Yannick Malbert. Flavonoid glucodiversification with engineered sucrose-active enzymes. Biotechnol- ogy. INSA de Toulouse, 2014. English. NNT : 2014ISAT0038. tel-01219406 HAL Id: tel-01219406 https://tel.archives-ouvertes.fr/tel-01219406 Submitted on 22 Oct 2015 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Last name: MALBERT First name: Yannick Title: Flavonoid glucodiversification with engineered sucrose-active enzymes Speciality: Ecological, Veterinary, Agronomic Sciences and Bioengineering, Field: Enzymatic and microbial engineering. Year: 2014 Number of pages: 257 Flavonoid glycosides are natural plant secondary metabolites exhibiting many physicochemical and biological properties. Glycosylation usually improves flavonoid solubility but access to flavonoid glycosides is limited by their low production levels in plants. In this thesis work, the focus was placed on the development of new glucodiversification routes of natural flavonoids by taking advantage of protein engineering. Two biochemically and structurally characterized recombinant transglucosylases, the amylosucrase from Neisseria polysaccharea and the α-(1→2) branching sucrase, a truncated form of the dextransucrase from L. Mesenteroides NRRL B-1299, were selected to attempt glucosylation of different flavonoids, synthesize new α-glucoside derivatives with original patterns of glucosylation and hopefully improved their water-solubility.
    [Show full text]
  • A Biosystematic Study of Allium Amplectens Torr
    University of the Pacific Scholarly Commons University of the Pacific Theses and Dissertations Graduate School 1974 A biosystematic study of Allium amplectens Torr Vickie Lynn Cain University of the Pacific Follow this and additional works at: https://scholarlycommons.pacific.edu/uop_etds Part of the Life Sciences Commons Recommended Citation Cain, Vickie Lynn. (1974). A biosystematic study of Allium amplectens Torr. University of the Pacific, Thesis. https://scholarlycommons.pacific.edu/uop_etds/1850 This Thesis is brought to you for free and open access by the Graduate School at Scholarly Commons. It has been accepted for inclusion in University of the Pacific Theses and Dissertations by an authorized administrator of Scholarly Commons. For more information, please contact [email protected]. A BIOSYSTEMI\'l'IC STUDY OF AlHum amplectens Torr. A 'lliesis Presented to The Faculty of the Department of Biological Sciences University of the Pacific In Partial Fulfillment of the Requirewents for the.Degree Master of Science in Biological Sciences by Vickie Lynn Cain August 1974 This thesis, written and submitted by is approved for recommendation to the Committee on Graduate Studies, University of the Pacific. Department Chairman or Dean: Chairman I; /') Date d c.~ cA; lfli ACKUOlvl.EDGSIV!EN'TS 'l'he author_ wishes to tha.'l.k Dr. B. Tdhelton a.YJ.d Dr. P. Gross for their• inva~i uoble advise and donations of time. l\'Iy appreciation to Dr. McNeal> my advisor. Expert assistance in the library vJEts pro:- vlded by Pr·, i:':I. SshaJit. To Vij c.y KJ12nna and Dolores No::..a.n rny ap-- preciatlon for rwraJ.
    [Show full text]
  • WO 2017/050853 Al 30 March 2017 (30.03.2017) P O P C T
    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/050853 Al 30 March 2017 (30.03.2017) P O P C T (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12P 19/44 (2006.01) C12N 15/52 (2006.01) kind of national protection available): AE, AG, AL, AM, C12P 17/06 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, (21) Number: International Application DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, PCT/EP20 16/072474 HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, (22) International Filing Date: KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 2 1 September 2016 (21 .09.201 6) MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (25) Filing Language: English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, (26) Publication Language: English TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 62/222,919 24 September 2015 (24.09.2015) US (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (71) Applicant: EVOLVA SA [CH/CH]; Duggingerstrasse 23, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, 4153 Reinach (CH).
    [Show full text]
  • EEE M W 24B 24A 27B 27A N Patent Application Publication Dec
    US 2009031 1494A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2009/0311494 A1 YAMASHTA et al. (43) Pub. Date: Dec. 17, 2009 (54) RELIEF PRINTING PLATE PRECURSOR FOR (30) Foreign Application Priority Data LASER ENGRAVING, RELIEF PRINTING PLATE, AND PROCESS FOR PRODUCING Jun. 17, 2008 (JP) ................................. 2008-157907 RELEF PRINTING PLATE Feb. 10, 2009 (JP) ................................. 2009-028816 (75) Inventors: Masako YAMASHITA, Publication Classification Shizuoka-ken (JP); Atsushi (51) Int. Cl. SUGASAKI, Shizuoka-ken (JP) B32B 3/00 (2006.01) Correspondence Address: GO3F 7/20 (2006.01) Moss & Burke, PLLC GO3F 7/004 (2006.01) 401 Holland Lane, Suite 407 Alexandria, VA 22314 (US) (52) U.S. Cl. .................... 428/195.1: 430/306: 430/286.1 (73) Assignee: FUJIFILM CORPORATION, (57) ABSTRACT Tokyo (JP) A relief printing plate precursor for laser engraving, including (21) Appl. No.: 12/476,260 a relief forming layer containing (A) a polymerizable com pound having an ethylenic unsaturated bond. (B) a binder (22) Filed: Jun. 2, 2009 polymer, and (C) a compound having deodorizing ability. 11 50 FA - 42 SUB SCANNING DIRECTION -10 - 228 7.s 55 21B EEE m w 24B 24A 27B 27A N Patent Application Publication Dec. 17, 2009 US 2009/0311494 A1 F.G. 1 FA SCANNING DIRECTION 7OA a. CSy ra & 5A - 27WSNS AD 23Ar S3EEASEE21 E-25sagaa EEEEEEEEEEEEEEEEEEEEEEEEE-22s awslighlights fskillsw. 21B 2 TTT "TT". US 2009/031 1494 A1 Dec. 17, 2009 RELEF PRINTING PLATE PRECURSORFOR mask to develop and remove an uncured area, and there is LASER ENGRAVING, RELIEF PRINTING room for improvement since development treatment is nec PLATE, AND PROCESS FOR PRODUCING essary.
    [Show full text]
  • 2. ACER Linnaeus, Sp. Pl. 2: 1054. 1753. 枫属 Feng Shu Trees Or Shrubs
    Fl. China 11: 516–553. 2008. 2. ACER Linnaeus, Sp. Pl. 2: 1054. 1753. 枫属 feng shu Trees or shrubs. Leaves mostly simple and palmately lobed or at least palmately veined, in a few species pinnately veined and entire or toothed, or pinnately or palmately 3–5-foliolate. Inflorescence corymbiform or umbelliform, sometimes racemose or large paniculate. Sepals (4 or)5, rarely 6. Petals (4 or)5, rarely 6, seldom absent. Stamens (4 or 5 or)8(or 10 or 12); filaments distinct. Carpels 2; ovules (1 or)2 per locule. Fruit a winged schizocarp, commonly a double samara, usually 1-seeded; embryo oily or starchy, radicle elongate, cotyledons 2, green, flat or plicate; endosperm absent. 2n = 26. About 129 species: widespread in both temperate and tropical regions of N Africa, Asia, Europe, and Central and North America; 99 species (61 endemic, three introduced) in China. Acer lanceolatum Molliard (Bull. Soc. Bot. France 50: 134. 1903), described from Guangxi, is an uncertain species and is therefore not accepted here. The type specimen, in Berlin (B), has been destroyed. Up to now, no additional specimens have been found that could help clarify the application of this name. Worldwide, Japanese maples are famous for their autumn color, and there are over 400 cultivars. Also, many Chinese maple trees have beautiful autumn colors and have been cultivated widely in Chinese gardens, such as Acer buergerianum, A. davidii, A. duplicatoserratum, A. griseum, A. pictum, A. tataricum subsp. ginnala, A. triflorum, A. truncatum, and A. wilsonii. In winter, the snake-bark maples (A. davidii and its relatives) and paper-bark maple (A.
    [Show full text]
  • Number 3, Spring 1998 Director’S Letter
    Planning and planting for a better world Friends of the JC Raulston Arboretum Newsletter Number 3, Spring 1998 Director’s Letter Spring greetings from the JC Raulston Arboretum! This garden- ing season is in full swing, and the Arboretum is the place to be. Emergence is the word! Flowers and foliage are emerging every- where. We had a magnificent late winter and early spring. The Cornus mas ‘Spring Glow’ located in the paradise garden was exquisite this year. The bright yellow flowers are bright and persistent, and the Students from a Wake Tech Community College Photography Class find exfoliating bark and attractive habit plenty to photograph on a February day in the Arboretum. make it a winner. It’s no wonder that JC was so excited about this done soon. Make sure you check of themselves than is expected to seedling selection from the field out many of the special gardens in keep things moving forward. I, for nursery. We are looking to propa- the Arboretum. Our volunteer one, am thankful for each and every gate numerous plants this spring in curators are busy planting and one of them. hopes of getting it into the trade. preparing those gardens for The magnolias were looking another season. Many thanks to all Lastly, when you visit the garden I fantastic until we had three days in our volunteers who work so very would challenge you to find the a row of temperatures in the low hard in the garden. It shows! Euscaphis japonicus. We had a twenties. There was plenty of Another reminder — from April to beautiful seven-foot specimen tree damage to open flowers, but the October, on Sunday’s at 2:00 p.m.
    [Show full text]
  • The Red List of Revised and Extended
    AcerThe Red List of revised and extended Dan Crowley, Megan Barstow, Malin Rivers & Yvette Harvey-Brown BOTANIC GARDENS CONSERVATION INTERNATIONAL (BGCI) is the world’s largest plant conservation network, comprising more than 500 botanic gardens in over 100 countries, and provides the secretariat to the IUCN/SSC Global Tree Specialist Group. BGCI was established in 1987 and is a registered charity with offices in the UK, US, China and Kenya. Published by Botanic Gardens Conservation International Descanso House, 199 Kew Road, Richmond, Surrey, TW9 3BW, UK. © 2020 Botanic Gardens Conservation International THE IUCN/SSC GLOBAL TREE SPECIALIST GROUP (GTSG) ISBN-10: 1-905164-74-2 ISBN-13: 978-1-905164-74-5 forms part of the Species Survival Commission’s network of over 7,000 volunteers working to stop the loss of plants, animals and their habitats. Reproduction of any part of the publication for SSC is the largest of the six Commissions of IUCN – The International educational, conservation and other non-profit purposes is authorized without prior permission from Union for Conservation of Nature. It serves as the main source of advice the copyright holder, provided that the source is fully to the Union and its members on the technical aspects of species acknowledged. conservation. The aims of the IUCN/SSC Global Tree Specialist Group Reproduction for resale or other commercial purposes are to promote and implement global red listing for trees and to act in is prohibited without prior written permission from the an advisory capacity to the Global Trees Campaign. copyright holder. Recommended citation: Crowley, D., Barstow, M., Rivers, M.
    [Show full text]
  • Wa Shan – Emei Shan, a Further Comparison
    photograph © Zhang Lin A rare view of Wa Shan almost minus its shroud of mist, viewed from the Abies fabri forested slopes of Emei Shan. At its far left the mist-filled Dadu River gorge drops to 500-600m. To its right the 3048m high peak of Mao Kou Shan climbed by Ernest Wilson on 3 July 1903. “As seen from the top of Mount Omei, it resembles a huge Noah’s Ark, broadside on, perched high up amongst the clouds” (Wilson 1913, describing Wa Shan floating in the proverbial ‘sea of clouds’). Wa Shan – Emei Shan, a further comparison CHRIS CALLAGHAN of the Australian Bicentennial Arboretum 72 updates his woody plants comparison of Wa Shan and its sister mountain, World Heritage-listed Emei Shan, finding Wa Shan to be deserving of recognition as one of the planet’s top hotspots for biological diversity. The founding fathers of modern day botany in China all trained at western institutions in Europe and America during the early decades of last century. In particular, a number of these eminent Chinese botanists, Qian Songshu (Prof. S. S. Chien), Hu Xiansu (Dr H. H. Hu of Metasequoia fame), Chen Huanyong (Prof. W. Y. Chun, lead author of Cathaya argyrophylla), Zhong Xinxuan (Prof. H. H. Chung) and Prof. Yung Chen, undertook their training at various institutions at Harvard University between 1916 and 1926 before returning home to estab- lish the initial Chinese botanical research institutions, initiate botanical exploration and create the earliest botanical gardens of China (Li 1944). It is not too much to expect that at least some of them would have had personal encounters with Ernest ‘Chinese’ Wilson who was stationed at the Arnold Arboretum of Harvard between 1910 and 1930 for the final 20 years of his life.
    [Show full text]
  • October 1961 , Volume 40, Number 4 305
    TIIE .A:M:ERICA.N ~GAZINE AMERICAN HORTICULTURAL SOCIETY A union of the Amej'ican HOTticu~tural Society and the AmeTican HOTticultural Council 1600 BLADENSBURG ROAD, NORTHEAST. WASHINGTON 2, D. C. For United Horticulture *** to accumulate, increase, and disseminate horticultural intOTmation B. Y. MORRISON, Editor Directors Terms Expiring 1961 JAMES R. HARLOW, Managing Editor STUART M. ARMSTRONG Maryland Editorial Committee JOH N L. CREECH . Maryland W. H . HODGE, Chairman WILLIAM H. FREDERICK, JR. Delaware JOH N L. CREECH FRANCIS PATTESON-KNIGHT FREDERIC P. LEE Virginia DONALD WYMAN CONRAD B. LINK Massachusetts CURTIS MAY T erms Expiring 1962 FREDERICK G . MEYER FREDERIC P. LEE WILBUR H . YOUNGMAN Maryland HENRY T . SKINNER District of Columbia OfJiceTS GEORGE H. SPALDING California PRESIDENT RICHARD P. WHITE DONAlJD WYMAN Distj'ict of Columbia Jamaica Plain, Massachusetts ANNE WERTSNER WOOD Pennsylvania FIRST VICE· PRESIDENT Ternu Expiring 1963 ALBERT J . IRVING New l'm'k, New York GRETCHEN HARSHBARGER Iowa SECOND VICE-PRESIDENT MARY W. M. HAKES Maryland ANNE WERTSNER W ' OOD FREDERIC HEUTTE Swarthmore, Pennsylvania Virginia W . H. HODGE SECRETARY-TREASURER OLIVE E. WEATHERELL ALBERT J . IRVING Washington, D, C. New York The Ame"ican Horticultural Magazine is the official publication of the American Horticultural Society and is issued four times a year during the quarters commencing with January, April, July and October. It is devoted to the dissemination of knowledge in the science and art of growing ornamental plants, fruits, vegetables, and related subjects. Original papers increasing the historical, varietal, and cultural knowledges of plant materials of economic and aesthetic importance are welcomed and will be published as early as possible.
    [Show full text]
  • ABSTRACT YUZUAK, SEYIT. Utilizing Metabolomics and Model Systems
    ABSTRACT YUZUAK, SEYIT. Utilizing Metabolomics and Model Systems to Gain Insight into Precursors and Polymerization of Proanthocyanidins in Plants (Under the direction of Dr. De-Yu Xie). Proanthocyanidins (PAs) are oligomers or polymers of flavan-3-ols. In plants, PAs have multiple protective functions against biotic and abiotic stresses. PAs are also important nutraceuticals existing in common beverages and food products to benefit human health. To date, although the biosynthesis of PAs has been intensively studieed and fundamental progress has been made in over the past decades, many questions remain unanswered. For example, its direct precursors of extension units are unknown and their monomer structure diversity are also unclear. These questions about proanthocyanidins result from not having of model systems and effective technologies. Our laboratory has made significant progress in enhancing the understanding of these unknown and unclear points regarding proanthocyanidins. In my dissertation research reported here, I focus on two areas: testing muscadine as a crop system to develop metabolomics for studying precursor diversity and isolating enzymes from a red cell tobacco system to understand the precursors of the extension units of PA. First, I developed a metabolomics protocol to analyze anthocyanidins and anthocyanins in muscadine grape. This study demonstrated that muscadine berries can produce at least six anthocyanidins, revealing the diversity of pathway precursors for PAs. The Journal of Agriculture and Food Chemistry is reviewing this work. Second, I developed a metabolomics protocol of HPLC-qTOF-MS/MS to analyze flavan- 3-ols and dimeric PAs in muscadine grape. This study also demonstrated the high structure diversity of flavan-3-ols, particularly methylated flavan-3-ols.
    [Show full text]
  • Interactions with Microbial Proteins Driving the Antibacterial Activity of Flavonoids
    pharmaceutics Review Interactions with Microbial Proteins Driving the Antibacterial Activity of Flavonoids Giuliana Donadio 1,†, Francesca Mensitieri 2,†, Valentina Santoro 1, Valentina Parisi 1,3, Maria Laura Bellone 1,3, Nunziatina De Tommasi 1, Viviana Izzo 2 and Fabrizio Dal Piaz 2,* 1 Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy; [email protected] (G.D.); [email protected] (V.S.); [email protected] (V.P.); [email protected] (M.L.B.); [email protected] (N.D.T.) 2 Department of Medicine and Surgery, University of Salerno, 84082 Baronissi, Italy; [email protected] (F.M.); [email protected] (V.I.) 3 PhD Program in Drug Discovery and Development, Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy * Correspondence: [email protected] † These authors contributed equally to this work. Abstract: Flavonoids are among the most abundant natural bioactive compounds produced by plants. Many different activities have been reported for these secondary metabolites against numerous cells and systems. One of the most interesting is certainly the antimicrobial, which is stimulated through various molecular mechanisms. In fact, flavonoids are effective both in directly damaging the envelope of Gram-negative and Gram-positive bacteria but also by acting toward specific molecular targets essential for the survival of these microorganisms. The purpose of this paper is to present an overview of the most interesting results obtained in the research focused on the study of the Citation: Donadio, G.; Mensitieri, F.; interactions between flavonoids and bacterial proteins. Despite the great structural heterogeneity Santoro, V.; Parisi, V.; Bellone, M.L.; of these plant metabolites, it is interesting to observe that many flavonoids affect the same cellular De Tommasi, N.; Izzo, V.; Dal Piaz, F.
    [Show full text]