ABSTRACT YUZUAK, SEYIT. Utilizing Metabolomics and Model Systems
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RP HPLC Determination of Some Uncommon Anthocyanins
360 УДК 543.54:547.814.5 RP HPLC determination of some uncommon anthocyanins Deineka V.I., Vu Thi Ngoc Anh, Deineka L.A. Federal State Autonomous Educational Institution of Higher Professional Education «Belgorod National Research University» RF, Belgorod Received 12.02.2014 Abstract Anthocyanins of Catharanthus roseus petals were found to be composed of pairs (3-galactosides and 3-rhamnosylgalactosides) mainly of 7-methylated anthocyanidins of “dephinidin series” (7-methyldelphinidin, 7-methylpetunidin and hirsutidin); though the same derivatives of “cyanidin series” are present in low concentrations. Flowers of Caesalpinia pulcherrima contain two 3-glucosides: of cyanidin and 5-methylcyanidin. The migration of CH 3-radical from 3’-OH group to 7-OH position results in increase of retention, while for the migration to 5-OH group a decrease of retention was observed. Different position of ring A OH-groups methylation can be predicted also by controversial shift of λmax of the solutes. Keywords: Reversed-phase HPLC, uncommon anthocyanins, 7-OH methylation; 5-OH methylation; regularities of retention alteration, electronic spectra . Установлено , что антоцианы лепестков цветков Catharanthus roseus образованы парами (3-галактозидами и 3-рамнозилгалактазидами ) в-основном антоцианидинами дельфинидинового ряда с метилированием OH-группы в положении 7: 7-метилдельфинидином , 7-метилпетунидином и 7- метилмальвидином ( хирсутидином ); впрочем , аналогичные производные антоцианов цианидинового ряда также присутствуют , но в небольших концентрациях . Антоцианы высушенных цветков Caesalpiniapulcherrima содержат два 3-глюкозида : цианидина и 5-метилцианидина . Перемещение CH 3-радикала из 3’-OH группы к 7-OH группе сказывается в заметном увеличении удерживания , но при аналогичном переносе на ОН -группу в положение 5 наблюдается уменьшение удерживания . Различный тип метилирования ОН -групп кольца А приводит к противоположным смещениям λmax антоцианов . -
Overexpression of Thmyc4e Enhances Anthocyanin Biosynthesis in Common Wheat
International Journal of Molecular Sciences Article Overexpression of ThMYC4E Enhances Anthocyanin Biosynthesis in Common Wheat 1,2,3, 1,2,3, 1,2,3 1,2 1,2,3 1,2,4, Shuo Zhao y, Xingyuan Xi y, Yuan Zong , Shiming Li , Yun Li , Dong Cao * and Baolong Liu 1,2,3,4,* 1 Qinghai Province Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China; [email protected] (S.Z.); [email protected] (X.X.); [email protected] (Y.Z.); [email protected] (S.L.); [email protected] (Y.L.) 2 Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China 3 College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China 4 The Innovative Academy of Seed Design, Chinese Academy of Sciences, Xining 810008, China * Correspondence: [email protected] (D.C.); [email protected] (B.L.) These authors contributed equally to this work. y Received: 28 November 2019; Accepted: 23 December 2019; Published: 24 December 2019 Abstract: The basic helix-loop helix (bHLH) transcription factor has been inferred to play an important role in blue and purple grain traits in common wheat, but to date, its overexpression has not been reported. In this study, the bHLH transcription factor ThMYC4E, the candidate gene controlling the blue grain trait from Th. Ponticum, was transferred to the common wheat JW1. The positive transgenic lines displayed higher levels of purple anthocyanin pigments in their grains, leaves and glumes. -
Chemistry and Pharmacology of Kinkéliba (Combretum
CHEMISTRY AND PHARMACOLOGY OF KINKÉLIBA (COMBRETUM MICRANTHUM), A WEST AFRICAN MEDICINAL PLANT By CARA RENAE WELCH A Dissertation submitted to the Graduate School-New Brunswick Rutgers, The State University of New Jersey in partial fulfillment of the requirements for the degree of Doctor of Philosophy Graduate Program in Medicinal Chemistry written under the direction of Dr. James E. Simon and approved by ______________________________ ______________________________ ______________________________ ______________________________ New Brunswick, New Jersey January, 2010 ABSTRACT OF THE DISSERTATION Chemistry and Pharmacology of Kinkéliba (Combretum micranthum), a West African Medicinal Plant by CARA RENAE WELCH Dissertation Director: James E. Simon Kinkéliba (Combretum micranthum, Fam. Combretaceae) is an undomesticated shrub species of western Africa and is one of the most popular traditional bush teas of Senegal. The herbal beverage is traditionally used for weight loss, digestion, as a diuretic and mild antibiotic, and to relieve pain. The fresh leaves are used to treat malarial fever. Leaf extracts, the most biologically active plant tissue relative to stem, bark and roots, were screened for antioxidant capacity, measuring the removal of a radical by UV/VIS spectrophotometry, anti-inflammatory activity, measuring inducible nitric oxide synthase (iNOS) in RAW 264.7 macrophage cells, and glucose-lowering activity, measuring phosphoenolpyruvate carboxykinase (PEPCK) mRNA expression in an H4IIE rat hepatoma cell line. Radical oxygen scavenging activity, or antioxidant capacity, was utilized for initially directing the fractionation; highlighted subfractions and isolated compounds were subsequently tested for anti-inflammatory and glucose-lowering activities. The ethyl acetate and n-butanol fractions of the crude leaf extract were fractionated leading to the isolation and identification of a number of polyphenolic ii compounds. -
Flavonoid Glucodiversification with Engineered Sucrose-Active Enzymes Yannick Malbert
Flavonoid glucodiversification with engineered sucrose-active enzymes Yannick Malbert To cite this version: Yannick Malbert. Flavonoid glucodiversification with engineered sucrose-active enzymes. Biotechnol- ogy. INSA de Toulouse, 2014. English. NNT : 2014ISAT0038. tel-01219406 HAL Id: tel-01219406 https://tel.archives-ouvertes.fr/tel-01219406 Submitted on 22 Oct 2015 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Last name: MALBERT First name: Yannick Title: Flavonoid glucodiversification with engineered sucrose-active enzymes Speciality: Ecological, Veterinary, Agronomic Sciences and Bioengineering, Field: Enzymatic and microbial engineering. Year: 2014 Number of pages: 257 Flavonoid glycosides are natural plant secondary metabolites exhibiting many physicochemical and biological properties. Glycosylation usually improves flavonoid solubility but access to flavonoid glycosides is limited by their low production levels in plants. In this thesis work, the focus was placed on the development of new glucodiversification routes of natural flavonoids by taking advantage of protein engineering. Two biochemically and structurally characterized recombinant transglucosylases, the amylosucrase from Neisseria polysaccharea and the α-(1→2) branching sucrase, a truncated form of the dextransucrase from L. Mesenteroides NRRL B-1299, were selected to attempt glucosylation of different flavonoids, synthesize new α-glucoside derivatives with original patterns of glucosylation and hopefully improved their water-solubility. -
A Biosystematic Study of Allium Amplectens Torr
University of the Pacific Scholarly Commons University of the Pacific Theses and Dissertations Graduate School 1974 A biosystematic study of Allium amplectens Torr Vickie Lynn Cain University of the Pacific Follow this and additional works at: https://scholarlycommons.pacific.edu/uop_etds Part of the Life Sciences Commons Recommended Citation Cain, Vickie Lynn. (1974). A biosystematic study of Allium amplectens Torr. University of the Pacific, Thesis. https://scholarlycommons.pacific.edu/uop_etds/1850 This Thesis is brought to you for free and open access by the Graduate School at Scholarly Commons. It has been accepted for inclusion in University of the Pacific Theses and Dissertations by an authorized administrator of Scholarly Commons. For more information, please contact [email protected]. A BIOSYSTEMI\'l'IC STUDY OF AlHum amplectens Torr. A 'lliesis Presented to The Faculty of the Department of Biological Sciences University of the Pacific In Partial Fulfillment of the Requirewents for the.Degree Master of Science in Biological Sciences by Vickie Lynn Cain August 1974 This thesis, written and submitted by is approved for recommendation to the Committee on Graduate Studies, University of the Pacific. Department Chairman or Dean: Chairman I; /') Date d c.~ cA; lfli ACKUOlvl.EDGSIV!EN'TS 'l'he author_ wishes to tha.'l.k Dr. B. Tdhelton a.YJ.d Dr. P. Gross for their• inva~i uoble advise and donations of time. l\'Iy appreciation to Dr. McNeal> my advisor. Expert assistance in the library vJEts pro:- vlded by Pr·, i:':I. SshaJit. To Vij c.y KJ12nna and Dolores No::..a.n rny ap-- preciatlon for rwraJ. -
WO 2017/050853 Al 30 March 2017 (30.03.2017) P O P C T
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/050853 Al 30 March 2017 (30.03.2017) P O P C T (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12P 19/44 (2006.01) C12N 15/52 (2006.01) kind of national protection available): AE, AG, AL, AM, C12P 17/06 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, (21) Number: International Application DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, PCT/EP20 16/072474 HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, (22) International Filing Date: KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 2 1 September 2016 (21 .09.201 6) MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (25) Filing Language: English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, (26) Publication Language: English TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 62/222,919 24 September 2015 (24.09.2015) US (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (71) Applicant: EVOLVA SA [CH/CH]; Duggingerstrasse 23, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, 4153 Reinach (CH). -
EEE M W 24B 24A 27B 27A N Patent Application Publication Dec
US 2009031 1494A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2009/0311494 A1 YAMASHTA et al. (43) Pub. Date: Dec. 17, 2009 (54) RELIEF PRINTING PLATE PRECURSOR FOR (30) Foreign Application Priority Data LASER ENGRAVING, RELIEF PRINTING PLATE, AND PROCESS FOR PRODUCING Jun. 17, 2008 (JP) ................................. 2008-157907 RELEF PRINTING PLATE Feb. 10, 2009 (JP) ................................. 2009-028816 (75) Inventors: Masako YAMASHITA, Publication Classification Shizuoka-ken (JP); Atsushi (51) Int. Cl. SUGASAKI, Shizuoka-ken (JP) B32B 3/00 (2006.01) Correspondence Address: GO3F 7/20 (2006.01) Moss & Burke, PLLC GO3F 7/004 (2006.01) 401 Holland Lane, Suite 407 Alexandria, VA 22314 (US) (52) U.S. Cl. .................... 428/195.1: 430/306: 430/286.1 (73) Assignee: FUJIFILM CORPORATION, (57) ABSTRACT Tokyo (JP) A relief printing plate precursor for laser engraving, including (21) Appl. No.: 12/476,260 a relief forming layer containing (A) a polymerizable com pound having an ethylenic unsaturated bond. (B) a binder (22) Filed: Jun. 2, 2009 polymer, and (C) a compound having deodorizing ability. 11 50 FA - 42 SUB SCANNING DIRECTION -10 - 228 7.s 55 21B EEE m w 24B 24A 27B 27A N Patent Application Publication Dec. 17, 2009 US 2009/0311494 A1 F.G. 1 FA SCANNING DIRECTION 7OA a. CSy ra & 5A - 27WSNS AD 23Ar S3EEASEE21 E-25sagaa EEEEEEEEEEEEEEEEEEEEEEEEE-22s awslighlights fskillsw. 21B 2 TTT "TT". US 2009/031 1494 A1 Dec. 17, 2009 RELEF PRINTING PLATE PRECURSORFOR mask to develop and remove an uncured area, and there is LASER ENGRAVING, RELIEF PRINTING room for improvement since development treatment is nec PLATE, AND PROCESS FOR PRODUCING essary. -
Transcriptomic and Metabolomic Joint Analysis Reveals Distinct Flavonoid Biosynthesis Regulation for Variegated Testa Color Deve
Transcriptomic and metabolomic joint analysis reveals distinct flavonoid biosynthesis regulation for variegated testa color development in peanut (Arachis hypogaea L.) Mengdie Hu1,Jiawei Li1, Mingyu Hou1,Xiaoqing Liu1,Shunli Cui1,Xinlei Yang1,Lifeng Liu1,Xiaoxia Jiang2, Guojun Mu1* 1.North China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of Agronomy, Hebei Agricultural University, Baoding, China 071001. 2.Hebei Yiyuan Ecological Agriculture Technology Co, Ltd, Baoding, China 074200 [email protected] (M.H.);[email protected] (J.L.);[email protected] (M.H.);[email protected] (X.L.); [email protected] (S.C.);[email protected] (L.Y.);[email protected] (L.L.);[email protected] (X.J.) * Correspondence:[email protected];Tel:+86-1380-326-0389 Abstract: Peanut (Arachis hypogaea L.) is one of the important oil and economic crops, among which the variegated testa peanut is a unique member. But the molecular mechanisms underlying the pigment synthesis in variegated testa are still unclear. Differentially expressed genes (DEGs) in pigment metabolism pathway in colored area indicated there were 27 DEGs highly related to the synthesis of variegated testa color among 1,050 DEGs,which were 13 up-regulated and 14 down-regulated,consisting of 3 PALs, 1 C4H, 2 CHSs, 1 F3H, 1 F3'H, 2 DFRs, 2 LARs, 2 IAAs, 4 bHLHs and 9 MYBs. GO analysis indicated DEGs were similarly enriched in 3 branches.KEGG analysis suggested flavonoid biosynthesis is the most direct metabolic pathway for the synthesis of testa variegation.The liquid chromatography tandem mass spectrometry (LC-MS/MS) results showed that cyanidin and delphinidin were the main metabolites that caused the color difference between the colored area and the non-colored area. -
Interactions with Microbial Proteins Driving the Antibacterial Activity of Flavonoids
pharmaceutics Review Interactions with Microbial Proteins Driving the Antibacterial Activity of Flavonoids Giuliana Donadio 1,†, Francesca Mensitieri 2,†, Valentina Santoro 1, Valentina Parisi 1,3, Maria Laura Bellone 1,3, Nunziatina De Tommasi 1, Viviana Izzo 2 and Fabrizio Dal Piaz 2,* 1 Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy; [email protected] (G.D.); [email protected] (V.S.); [email protected] (V.P.); [email protected] (M.L.B.); [email protected] (N.D.T.) 2 Department of Medicine and Surgery, University of Salerno, 84082 Baronissi, Italy; [email protected] (F.M.); [email protected] (V.I.) 3 PhD Program in Drug Discovery and Development, Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy * Correspondence: [email protected] † These authors contributed equally to this work. Abstract: Flavonoids are among the most abundant natural bioactive compounds produced by plants. Many different activities have been reported for these secondary metabolites against numerous cells and systems. One of the most interesting is certainly the antimicrobial, which is stimulated through various molecular mechanisms. In fact, flavonoids are effective both in directly damaging the envelope of Gram-negative and Gram-positive bacteria but also by acting toward specific molecular targets essential for the survival of these microorganisms. The purpose of this paper is to present an overview of the most interesting results obtained in the research focused on the study of the Citation: Donadio, G.; Mensitieri, F.; interactions between flavonoids and bacterial proteins. Despite the great structural heterogeneity Santoro, V.; Parisi, V.; Bellone, M.L.; of these plant metabolites, it is interesting to observe that many flavonoids affect the same cellular De Tommasi, N.; Izzo, V.; Dal Piaz, F. -
Purple Anthocyanin Colouration on Lower (Abaxial) Leaf Surface of Hemigraphis Colorata (Acanthaceae)
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by NORA - Norwegian Open Research Archives Phytochemistry 105 (2014) 141–146 Contents lists available at ScienceDirect Phytochemistry journal homepage: www.elsevier.com/locate/phytochem Purple anthocyanin colouration on lower (abaxial) leaf surface of Hemigraphis colorata (Acanthaceae) Irene Skaar a, Christopher Adaku b, Monica Jordheim a, Robert Byamukama b, Bernard Kiremire b, ⇑ Øyvind M. Andersen a, a Department of Chemistry, University of Bergen, Allégt. 41, 5007 Bergen, Norway b Chemistry Department, Makerere University, P.O. Box 7062, Kampala, Uganda article info abstract Article history: The functional significance of anthocyanin colouration of lower (abaxial) leaf surfaces is not clear. Received 1 August 2013 Two anthocyanins, 5-O-methylcyanidin 3-O-(300-(b-glucuronopyranosyl)-b-glucopyranoside) (1) and Received in revised form 19 May 2014 5-O-methylcyanidin 3-O-b-glucopyranoside (2), were isolated from Hemigraphis colorata (Blume) Available online 20 June 2014 (Acanthaceae) leaves with strong purple abaxial colouration (2.2 and 0.6 mg/g fr. wt., respectively). The glycosyl moiety of 1, the disaccharide 300-(b-glucuronopyranosyl)-b-glucopyranoside), has previously Keywords: been reported to occur only in a triterpenoid saponin, lindernioside A. The structural assignment of Hemigraphis colorata the aglycone of 1 and 2 is the first complete characterisation of a natural 7-hydroxy-5-methoxyanthocy- Acanthaceae anidin. Compared to nearly all naturally occurring anthocyanidins, the 5-O-methylation of this anthocy- Abaxial leaf colouration Anthocyanins anidin limits the type of possible quinoidal forms of 1 and 2 to be those forms with keto-function in only 0 5-O-Methylcyanidin their 7- and 4 -positions. -
Ep 3138585 A1
(19) TZZ¥_¥_T (11) EP 3 138 585 A1 (12) EUROPEAN PATENT APPLICATION (43) Date of publication: (51) Int Cl.: 08.03.2017 Bulletin 2017/10 A61L 27/20 (2006.01) A61L 27/54 (2006.01) A61L 27/52 (2006.01) (21) Application number: 16191450.2 (22) Date of filing: 13.01.2011 (84) Designated Contracting States: (72) Inventors: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB • Gousse, Cecile GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO 74230 Dingy Saint Clair (FR) PL PT RO RS SE SI SK SM TR • Lebreton, Pierre Designated Extension States: 74000 Annecy (FR) BA ME •Prost,Nicloas 69440 Mornant (FR) (30) Priority: 13.01.2010 US 687048 26.02.2010 US 714377 (74) Representative: Hoffmann Eitle 30.11.2010 US 956542 Patent- und Rechtsanwälte PartmbB Arabellastraße 30 (62) Document number(s) of the earlier application(s) in 81925 München (DE) accordance with Art. 76 EPC: 15178823.9 / 2 959 923 Remarks: 11709184.3 / 2 523 701 This application was filed on 29-09-2016 as a divisional application to the application mentioned (71) Applicant: Allergan Industrie, SAS under INID code 62. 74370 Pringy (FR) (54) STABLE HYDROGEL COMPOSITIONS INCLUDING ADDITIVES (57) The present specification generally relates to hydrogel compositions and methods of treating a soft tissue condition using such hydrogel compositions. EP 3 138 585 A1 Printed by Jouve, 75001 PARIS (FR) EP 3 138 585 A1 Description CROSS REFERENCE 5 [0001] This patent application is a continuation-in-part of U.S. -
Transcriptomic and Metabolomic Joint Analysis Reveals Distinct Flavonoid Biosynthesis Regulation for Variegated Testa Color Deve
www.nature.com/scientificreports OPEN Transcriptomic and metabolomic joint analysis reveals distinct favonoid biosynthesis regulation for variegated testa color development in peanut (Arachis hypogaea L.) Mengdie Hu1, Jiawei Li1, Mingyu Hou1, Xiaoqing Liu1, Shunli Cui1, Xinlei Yang1, Lifeng Liu1, Xiaoxia Jiang2 & Guojun Mu1* Peanut is one of the important oil and economic crops, among which the variegated testa peanut is a unique member. The molecular mechanisms underlying the pigment synthesis in variegated testa are still unclear. Diferentially expressed genes (DEGs) in the favonoid metabolism pathway in pigmented areas indicated that there were 27 DEGs highly related to the synthesis of variegated testa color among 1,050 DEGs. Of these 27, 13 were up-regulated and 14 were down-regulated, including 3 PALs, 1 C4H, 2 CHSs, 1 F3H, 1 F3’H, 2 DFRs, 2 LARs, 2 IAAs, 4 bHLHs, and 9 MYBs. GO (Gene Ontology) analysis indicated that DEGs were similarly enriched in three branches. KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis suggested favonoid biosynthesis is the most direct metabolic pathway for the synthesis of testa variegation. The liquid chromatography–tandem mass spectrometry (LC–MS/ MS) results showed that cyanidin and delphinidin were the primary metabolites that caused the color diferences between the pigmented and the non-pigmented areas. Through the verifcation of 20 DEGs via qPCR, the results were consistent with transcriptome sequencing in four comparison groups. The results in this study lay the foundation for revealing the molecular regulation mechanisms of favonoid synthesis in variegated testa peanut. Peanut (Arachis hypogaea L.) is the primary oil and economic crop that occupies an important position in the world for grain, oil, and food.