RP HPLC Determination of Some Uncommon Anthocyanins
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Chemistry and Pharmacology of Kinkéliba (Combretum
CHEMISTRY AND PHARMACOLOGY OF KINKÉLIBA (COMBRETUM MICRANTHUM), A WEST AFRICAN MEDICINAL PLANT By CARA RENAE WELCH A Dissertation submitted to the Graduate School-New Brunswick Rutgers, The State University of New Jersey in partial fulfillment of the requirements for the degree of Doctor of Philosophy Graduate Program in Medicinal Chemistry written under the direction of Dr. James E. Simon and approved by ______________________________ ______________________________ ______________________________ ______________________________ New Brunswick, New Jersey January, 2010 ABSTRACT OF THE DISSERTATION Chemistry and Pharmacology of Kinkéliba (Combretum micranthum), a West African Medicinal Plant by CARA RENAE WELCH Dissertation Director: James E. Simon Kinkéliba (Combretum micranthum, Fam. Combretaceae) is an undomesticated shrub species of western Africa and is one of the most popular traditional bush teas of Senegal. The herbal beverage is traditionally used for weight loss, digestion, as a diuretic and mild antibiotic, and to relieve pain. The fresh leaves are used to treat malarial fever. Leaf extracts, the most biologically active plant tissue relative to stem, bark and roots, were screened for antioxidant capacity, measuring the removal of a radical by UV/VIS spectrophotometry, anti-inflammatory activity, measuring inducible nitric oxide synthase (iNOS) in RAW 264.7 macrophage cells, and glucose-lowering activity, measuring phosphoenolpyruvate carboxykinase (PEPCK) mRNA expression in an H4IIE rat hepatoma cell line. Radical oxygen scavenging activity, or antioxidant capacity, was utilized for initially directing the fractionation; highlighted subfractions and isolated compounds were subsequently tested for anti-inflammatory and glucose-lowering activities. The ethyl acetate and n-butanol fractions of the crude leaf extract were fractionated leading to the isolation and identification of a number of polyphenolic ii compounds. -
ABSTRACT YUZUAK, SEYIT. Utilizing Metabolomics and Model Systems
ABSTRACT YUZUAK, SEYIT. Utilizing Metabolomics and Model Systems to Gain Insight into Precursors and Polymerization of Proanthocyanidins in Plants (Under the direction of Dr. De-Yu Xie). Proanthocyanidins (PAs) are oligomers or polymers of flavan-3-ols. In plants, PAs have multiple protective functions against biotic and abiotic stresses. PAs are also important nutraceuticals existing in common beverages and food products to benefit human health. To date, although the biosynthesis of PAs has been intensively studieed and fundamental progress has been made in over the past decades, many questions remain unanswered. For example, its direct precursors of extension units are unknown and their monomer structure diversity are also unclear. These questions about proanthocyanidins result from not having of model systems and effective technologies. Our laboratory has made significant progress in enhancing the understanding of these unknown and unclear points regarding proanthocyanidins. In my dissertation research reported here, I focus on two areas: testing muscadine as a crop system to develop metabolomics for studying precursor diversity and isolating enzymes from a red cell tobacco system to understand the precursors of the extension units of PA. First, I developed a metabolomics protocol to analyze anthocyanidins and anthocyanins in muscadine grape. This study demonstrated that muscadine berries can produce at least six anthocyanidins, revealing the diversity of pathway precursors for PAs. The Journal of Agriculture and Food Chemistry is reviewing this work. Second, I developed a metabolomics protocol of HPLC-qTOF-MS/MS to analyze flavan- 3-ols and dimeric PAs in muscadine grape. This study also demonstrated the high structure diversity of flavan-3-ols, particularly methylated flavan-3-ols. -
Purple Anthocyanin Colouration on Lower (Abaxial) Leaf Surface of Hemigraphis Colorata (Acanthaceae)
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by NORA - Norwegian Open Research Archives Phytochemistry 105 (2014) 141–146 Contents lists available at ScienceDirect Phytochemistry journal homepage: www.elsevier.com/locate/phytochem Purple anthocyanin colouration on lower (abaxial) leaf surface of Hemigraphis colorata (Acanthaceae) Irene Skaar a, Christopher Adaku b, Monica Jordheim a, Robert Byamukama b, Bernard Kiremire b, ⇑ Øyvind M. Andersen a, a Department of Chemistry, University of Bergen, Allégt. 41, 5007 Bergen, Norway b Chemistry Department, Makerere University, P.O. Box 7062, Kampala, Uganda article info abstract Article history: The functional significance of anthocyanin colouration of lower (abaxial) leaf surfaces is not clear. Received 1 August 2013 Two anthocyanins, 5-O-methylcyanidin 3-O-(300-(b-glucuronopyranosyl)-b-glucopyranoside) (1) and Received in revised form 19 May 2014 5-O-methylcyanidin 3-O-b-glucopyranoside (2), were isolated from Hemigraphis colorata (Blume) Available online 20 June 2014 (Acanthaceae) leaves with strong purple abaxial colouration (2.2 and 0.6 mg/g fr. wt., respectively). The glycosyl moiety of 1, the disaccharide 300-(b-glucuronopyranosyl)-b-glucopyranoside), has previously Keywords: been reported to occur only in a triterpenoid saponin, lindernioside A. The structural assignment of Hemigraphis colorata the aglycone of 1 and 2 is the first complete characterisation of a natural 7-hydroxy-5-methoxyanthocy- Acanthaceae anidin. Compared to nearly all naturally occurring anthocyanidins, the 5-O-methylation of this anthocy- Abaxial leaf colouration Anthocyanins anidin limits the type of possible quinoidal forms of 1 and 2 to be those forms with keto-function in only 0 5-O-Methylcyanidin their 7- and 4 -positions. -
Ep 3138585 A1
(19) TZZ¥_¥_T (11) EP 3 138 585 A1 (12) EUROPEAN PATENT APPLICATION (43) Date of publication: (51) Int Cl.: 08.03.2017 Bulletin 2017/10 A61L 27/20 (2006.01) A61L 27/54 (2006.01) A61L 27/52 (2006.01) (21) Application number: 16191450.2 (22) Date of filing: 13.01.2011 (84) Designated Contracting States: (72) Inventors: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB • Gousse, Cecile GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO 74230 Dingy Saint Clair (FR) PL PT RO RS SE SI SK SM TR • Lebreton, Pierre Designated Extension States: 74000 Annecy (FR) BA ME •Prost,Nicloas 69440 Mornant (FR) (30) Priority: 13.01.2010 US 687048 26.02.2010 US 714377 (74) Representative: Hoffmann Eitle 30.11.2010 US 956542 Patent- und Rechtsanwälte PartmbB Arabellastraße 30 (62) Document number(s) of the earlier application(s) in 81925 München (DE) accordance with Art. 76 EPC: 15178823.9 / 2 959 923 Remarks: 11709184.3 / 2 523 701 This application was filed on 29-09-2016 as a divisional application to the application mentioned (71) Applicant: Allergan Industrie, SAS under INID code 62. 74370 Pringy (FR) (54) STABLE HYDROGEL COMPOSITIONS INCLUDING ADDITIVES (57) The present specification generally relates to hydrogel compositions and methods of treating a soft tissue condition using such hydrogel compositions. EP 3 138 585 A1 Printed by Jouve, 75001 PARIS (FR) EP 3 138 585 A1 Description CROSS REFERENCE 5 [0001] This patent application is a continuation-in-part of U.S. -
Anthocyanin Pigments: Beyond Aesthetics
molecules Review Anthocyanin Pigments: Beyond Aesthetics , Bindhu Alappat * y and Jayaraj Alappat y Warde Academic Center, St. Xavier University, 3700 W 103rd St, Chicago, IL 60655, USA; [email protected] * Correspondence: [email protected] These authors contributed equally to this work. y Academic Editor: Pasquale Crupi Received: 29 September 2020; Accepted: 19 November 2020; Published: 24 November 2020 Abstract: Anthocyanins are polyphenol compounds that render various hues of pink, red, purple, and blue in flowers, vegetables, and fruits. Anthocyanins also play significant roles in plant propagation, ecophysiology, and plant defense mechanisms. Structurally, anthocyanins are anthocyanidins modified by sugars and acyl acids. Anthocyanin colors are susceptible to pH, light, temperatures, and metal ions. The stability of anthocyanins is controlled by various factors, including inter and intramolecular complexations. Chromatographic and spectrometric methods have been extensively used for the extraction, isolation, and identification of anthocyanins. Anthocyanins play a major role in the pharmaceutical; nutraceutical; and food coloring, flavoring, and preserving industries. Research in these areas has not satisfied the urge for natural and sustainable colors and supplemental products. The lability of anthocyanins under various formulated conditions is the primary reason for this delay. New gene editing technologies to modify anthocyanin structures in vivo and the structural modification of anthocyanin via semi-synthetic methods offer new opportunities in this area. This review focusses on the biogenetics of anthocyanins; their colors, structural modifications, and stability; their various applications in human health and welfare; and advances in the field. Keywords: anthocyanins; anthocyanidins; biogenetics; polyphenols; flavonoids; plant pigments; anthocyanin bioactivities 1. Introduction Anthocyanins are water soluble pigments that occur in most vascular plants. -
Wo 2009/063333 A2
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date (10) International Publication Number 22 May 2009 (22.05.2009) PCT WO 2009/063333 A2 (51) International Patent Classification: Not classified (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, (21) International Application Number: AO, AT,AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, PCT/IB2008/003890 CH, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, (22) International Filing Date: IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, 14 November 2008 (14.1 1.2008) LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, (25) Filing Language: English RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY,TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, (26) Publication Language: English ZW (84) Designated States (unless otherwise indicated, for every (30) Priority Data: kind of regional protection available): ARIPO (BW, GH, 60/987,866 14 November 2007 (14.1 1.2007) US GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, 61/037,191 17 March 2008 (17.03.2008) US ZW), Eurasian (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM), 12/270,23 1 13 November 2008 (13.11.2008) US European (AT,BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT,LU, LV,MC, MT, NL, (71) Applicant (for all designated States except US): OMNICA NO, PL, PT, RO, SE, SI, SK, TR), OAPI (BF, BJ, CF, CG, GMBH [DE/DE]; Plan 5, 20095 Hamburg (DE). -
Some Reactions of Anthocyanin Pigments
/ ^omc/ly BA1.0S k , HOOKBINniNG f- HiiRvicr I tfd HHONn HQAO Ok.BnNiii.juMcrios K Permission has been granted by the Head of the School in which this thesis was submitted for it to be consulted ^^^ and copied This permission is contained in the Administration file "AYailability of H.D. Theses" and applies only to those theses lodged with the University before the use of Disposition Declaration f orms, SOME REACTIONS OP ANTEOCYAKIlf PIGMENTS A Thesis submitted for the degree of Master of Science of the University of New South Wales hy Kevin Anthony Harper B.Sc. (Hons.) Submitted January 1961 UNIVERSITY OF N.SJ. 27878 26. FEB.75 LIBRARY DECLARATION The candidate, Kevin Anthony Harper, hereby declares that none of the work presented in this Thesis has "been submitted to any other University or Institution for a higher degree. AOKNOWLEDG-MENTS The author wishes to record this thanks to Dr. E. R. Cole of the UniTersity of New South Wales and to Mr. J. P. Kefford of the C.S.I,R»0, Bivision of Pood Preservation for their consider- able help and guidance in the work described in this Thesis. He is also deeply indebted to Dr. J. R. Vickery, Chief of the O.S.I.R.O. Division of Pood Preservation, for permission to carry out this work in the Division's laboratories. He also wishes to express his gratitude to Dr. B. V. Chandler for many helpful criticisms and discussions of the work and to Mr. D. J. Oasimir for his assistance in settin^g up the polarograph and instruction in the technique of polarography. -
Transcriptome Sequencing and Metabolite Analysis for Revealing
Wu et al. BMC Genomics (2016) 17:897 DOI 10.1186/s12864-016-3226-9 RESEARCH ARTICLE Open Access Transcriptome sequencing and metabolite analysis for revealing the blue flower formation in waterlily Qian Wu1,2, Jie Wu1,2, Shan-Shan Li1*, Hui-Jin Zhang1, Cheng-Yong Feng1,2, Dan-Dan Yin1,2, Ru-Yan Wu1,3 and Liang-Sheng Wang1* Abstract Background: Waterlily (Nymphaea spp.), a perennial herbaceous aquatic plant, is divided into two ecological groups: hardy waterlily and tropical waterlily. Although the hardy waterlily has no attractive blue flower cultivar, its adaptability is stronger than tropical waterlily because it can survive a cold winter. Thus, breeding hardy waterlily with real blue flowers has become an important target for breeders. Molecular breeding may be a useful way. However, molecular studies on waterlily are limited due to the lack of sequence data. Results: In this study, six cDNA libraries generated from the petals of two different coloring stages of blue tropical waterlily cultivar Nymphaea ‘King of Siam’ were sequenced using the Illumina HiSeq™ 2500 platform. Each library produced no less than 5.65 Gb clean reads. Subsequently, de novo assembly generated 112,485 unigenes, including 26,206 unigenes annotated to seven public protein databases. Then, 127 unigenes could be identified as putative homologues of color-related genes in other species, including 28 up-regulated and 5 down-regulated unigenes. In petals, 16 flavonoids (4 anthocyanins and 12 flavonols) were detected in different contents during the color development due to the different expression levels of color-related genes, and four flavonols were detected in waterlily for the first time. -
The PLANT PHENOLIC COMPOUNDS Introduction & the Flavonoids the Plant Phenolic Compounds - 8,000 Phenolic Structures Known
The PLANT PHENOLIC COMPOUNDS Introduction & The Flavonoids The plant phenolic compounds - 8,000 Phenolic structures known - Account for 40% of organic carbon circulating in the biosphere - Evolution of vascular plants: in cell wall structures, plant defense, features of woods and barks, flower color, flavors The plant phenolic compounds They can be: Simple, low molecular weight, single aromatic ringed compounds TO- Large and complex- polyphenols The Plant phenolic compounds The plant phenolic compounds - Primarily derived from the: Phenylpropanoid pathway and acetate pathway (and related pathways) Phenylpropanoid pathway and phenylpropanoid- acetate pathway Precursors for plant phenolic compounds The phenylpropanoids: products of the shikimic acid pathway The phenylpropanoids: products of the shikimic acid pathway (phe and tyr) THE PHENYLPROPANOIDS: PRODUCTS OF THE SHIKIMIC ACID PATHWAY (phe & tyr) The shikimate pathway The plant phenolic compounds - As in other cases of SMs, branches of pathway leading to biosynthesis of phenols are found or amplified only in specific plant families - Commonly found conjugated to sugars and organic acids The plant phenolic compounds Phenolics can be classified into 2 groups: 1. The FLAVONOIDS 2. The NON-FLAVONOIDS The plant phenolic compounds THE FLAVONOIDS - Polyphenolic compounds - Comprise: 15 carbons + 2 aromatic rings connected with a 3 carbon bridge The Flavane Nucleus THE FLAVONOIDS - Largest group of phenols: 4500 - Major role in plants: color, pathogens, light stress - Very often in epidermis of leaves and fruit skin - Potential health promoting compounds- antioxidants - A large number of genes known THE Flavonoids- classes Lepiniec et al., 2006 THE Flavonoids - The basic flavonoid skeleton can have a large number of substitutions on it: - Hydroxyl groups - Sugars - e.g. -
Studies on the Anthocyanin Pigments in Allium Amplectens Torr
University of the Pacific Scholarly Commons University of the Pacific Theses and Dissertations Graduate School 1972 Studies on the anthocyanin pigments in Allium amplectens Torr Chun-Mei Chu University of the Pacific Follow this and additional works at: https://scholarlycommons.pacific.edu/uop_etds Part of the Biology Commons Recommended Citation Chu, Chun-Mei. (1972). Studies on the anthocyanin pigments in Allium amplectens Torr. University of the Pacific, Thesis. https://scholarlycommons.pacific.edu/uop_etds/1787 This Thesis is brought to you for free and open access by the Graduate School at Scholarly Commons. It has been accepted for inclusion in University of the Pacific Theses and Dissertations by an authorized administrator of Scholarly Commons. For more information, please contact [email protected]. STUDIES ON THE ANTHOCYANIN" PIGHEHTS IN Allium amplec~ ']~orr. A Thesis Presented to The Faculty of the Department of Biological Sciences University of the Pacific In Partial Fulfillment of the Requirements for the Degree r1aster of Science in Biological Sciences by Chun-Nei Chu December 1972 This thesis, written and submitted by is approved for recommendation to the Committee on Graduate Studies, University of the Pacific. Department Chairman or Dean: Thesis Chairman -~· ACKNOWLEDGEMENT I am deeply grateful to Dr.o Dale W. ~1cNea.l of the University of the Pacific under whose guidance this study was made. I would also like to thank 11r. John P. IvlcGo'.'ran for his help. -~~- .. · .. ' .. :~ .. TABLE OF CONTENTS PAGE INTRODUCTION . .. .. .. .. .. .. 1 LITERATURE REVIillv General review of anthocyanins ••••••••••••••••••••• 5 Isolation ~nd identification ••••••••••••••••••••••• 10 Biosynthesis of anthocyanins .. .. .. .. .. .. 11 MATERIALS AND NETHODS .... ~ .. ~ ..........• ............. 15 RESULTS .••• 6 • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • ~ • • • • • • 19 DISCUSSION .................................... -
Istanbul Technical University Graduate School Of
ISTANBUL TECHNICAL UNIVERSITY GRADUATE SCHOOL OF SCIENCE ENGINEERING AND TECHNOLOGY FOOD MATRIX EFFECT ON BIOAVAILABILITY OF PHENOLICS AND ANTHOCYANINS IN SOUR CHERRY M.Sc. THESIS Tuğba ÖKSÜZ Department of Food Engineering Food Engineering Programme Anabilim Dalı : Herhangi Mühendislik, Bilim Programı : Herhangi Program JUNE 2013 ISTANBUL TECHNICAL UNIVERSITY GRADUATE SCHOOL OF SCIENCE ENGINEERING AND TECHNOLOGY FOOD MATRIX EFFECT ON BIOAVAILABILITY OF PHENOLICS AND ANTHOCYANINS IN SOUR CHERRY M.Sc. THESIS Tuğba ÖKSÜZ (506111523) Department of Food Engineering Food Engineering Programme Thesis Advisor: Assist. Prof. Dilara NİLÜFER-ERDİL Anabilim Dalı : Herhangi Mühendislik, Bilim Programı : Herhangi Program JUNE 2013 İSTANBUL TEKNİK ÜNİVERSİTESİ FEN BİLİMLERİ ENSTİTÜSÜ VİŞNEDE BULUNAN FENOLİK BİLEŞENLERİN VE ANTOSİYANİNLERİN BİYOYARARLILIĞINA GIDA MATRİSİ VE BİLEŞENLERİN ETKİSİ YÜKSEK LİSANS TEZİ Tuğba ÖKSÜZ (506111523) Gıda Mühendisliği Anabilim Dalı Gıda Mühendisliği Programı Tez Danışmanı: Yrd. Doç. Dr. Dilara NİLÜFER-ERDİL Anabilim Dalı : Herhangi Mühendislik, Bilim Programı : Herhangi Program HAZİRAN 2013 Tuğba ÖKSÜZ, a M.Sc. student of ITU Graduate School of Food Engineering student ID 506111523, successfully defended the thesis entitled “Food matrix effect on bioavailability of phenolics and anthocyanins in sour cherry”, which she prepared after fulfilling the requirements specified in the associated legislations, before the jury whose signatures are below. Thesis Advisor : Assist. Prof. Dilara NİLÜFER-ERDİL .............................. İstanbul Technical University Jury Members : Assist. Prof. Neşe ŞAHİN-YEŞİLÇUBUK ............................ İstanbul Technical University Assist. Prof. Murat TAŞAN .............................. Namık Kemal University Date of Submission : 03 May 2013 Date of Defense : 05 June 2013 v vi To my family, vii viii FOREWORD Sour cherry is one of the special fruits for Turkey who is in the leader producer position in the world. -
ABSTRACT Title of Dissertation / Thesis
ABSTRACT Title of Dissertation / Thesis: ABSORPTION, EXCRETION, AND TRANSFORMATION OF INDIVIDUAL ANTHOCYANINS IN RATS Jian He, Master of Science, 2004 Dissertation / Thesis Directed By: Assistant Professor M. Monica Giusti Department of Nutr ition and Food Science Anthocyanins are poly phenolics responsible for most red to purple colors in plants. Human consumption is increasing because of their potential health benefits and use as natural colorants . However, their absorption and metabolism are not well characterized. We compared anthocyanin absorption and excretion in rats receiving chokeberry, bilberry or grape enriched diet (4g anthocyanin/kg) for 13 wee ks. Trace s of anthocyanins and metabolites were detected in plasma. In urine, intact an thocyanins and methylated derivatives ( ~ 24, 8, 1 5 mg cy -3-gla equivalent/L urine for chokeberry, bilberry, grape) were found. High metabolite concentration suggested accumulation of methylated anthocyanins in tissue. Fecal anthocyanin extraction was maxim ized with aqueous methanol (60%). Anthocyanin concentration in feces rang ed from 0.7 to 2g anthocyanin/kg, similar to cecal content. In the gut, anthocyanin degradation was high for glucosides, moderate for galactosides and negligible for arabinosides and xylosides . Both , glycosylation and acylation seemed to affect the bioavailability of anthocyanins in vivo . ABSORPTION, EXCRETION, AND TRANSFORMATION OF INDIVIDUAL ANTHOCYANINS IN RATS By Jian He Thesis submitted to the Faculty of th e Graduate School of the University of Maryland, College Park, in partial fulfillment of the requirements for the degree of Master of Science 2004 Advisory Committee: Assistant Professor M. Monica Giusti, Chair (Advisor) Assistant Professor Bern adene A. Magnuson (Co -advisor) Assistant Professor Liangli Yu © Copyright by Jian He 2004 Dedication The thesis is dedicated to my beloved father Shide He and mother Zonghui Hu, for supporting my education, and to my dear wife Weishu Xue, from whom the time devoted to this thesis has been withdrawn.