Musselshell Watershed Water Quality Monitoring Project — 2015: Nutrients, Metals and E

MUSSELSHELL WATERSHED WATER QUALITY MONITORING PROJECT — 2015: NUTRIENTS, METALS AND E. COLI

Sampling and Analysis Plan

Prepared for:

MONTANA DEPARTMENT OF ENVIRONMENTAL QUALITY Water Quality Monitoring and Assessment and Water Quality Management Sections Water Quality Planning Bureau P.O. Box 200901 Helena, MT 59620-0901

Approvals

______Kathryn Makarowski (WQ Monitoring & Assessment Section) Date

______Darrin Kron (WQ Monitoring & Assessment Section Manager) Date

______Terri Mavencamp (Quality Assurance Officer) Date

______Jordan Tollefson (Musselshell Project Coordinator) Date

______Lou Volpe (TMDL Project Manager) Date

______Dean Yashan (Watershed Management Section Manager) Date

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2 WQPBMASSAP-19 1.0 INTRODUCTION AND BACKGROUND INFORMATION

1.1 PROJECT AREA DESCRIPTION The Musselshell River basin (Figure 1.1) is located in central-eastern Montana and is comprised of 5 major (4th code HUC) sub-basins, shown in Table 1.1. The basin spans 11 counties, primarily Meagher, Wheatland, Golden Valley, Musselshell, Fergus and Petroleum (also Sweetgrass, Stillwater, Yellowstone, Rosebud and Garfield).

The Musselshell River is approximately 340 miles long and originates at the confluence of the North Fork Musselshell River (which drains from the Little Belt and Castle mountains northeast of White Sulphur Springs), and the South Fork Musselshell River (which drains from the Castle and Crazy Mountains southeast of White Sulphur Springs). It flows generally east through towns including Two Dot, Harlowton, Shawmut, Ryegate and Roundup, where the Big Snowy Mountains lie to the North and the Bull Mountains lie to the South. The river continues northeast through Musselshell and, near Melstone, flows north through Mosby and into Fort Peck Reservoir. The river is split into four assessment units, as shown in Table 1.2.

Figure 1.1. Musselshell River watershed with sub-basins and counties.

There are 51 watersheds (5th code HUC) within the Musselshell River watershed that were considered for water quality characterization or assessment. Several sub-watersheds (6th code HUC) were also considered due to existing impairment listings or because of their high resource value. A majority of the streams draining these watersheds have C-3 use classifications and are located within the Northwestern

3 WQPBMASSAP-19 Great Plains level III ecoregion. Several tributaries in the upper basin are classified B-1; these waters’ headwaters originate in the Middle Rockies level III ecoregion and transition to the Northwestern Great Plains ecoregion lower in their drainage. As shown in Table 1.2, the uppermost segment of the Musselshell River, from the confluence of the North and South forks to the Deadmans Basin Diversion Canal, has a B-2 use classification; the remaining segments are classified C-3.

Table 1.1 Major sub-basins and HUCs in Musselshell. Sub-Basin Hydrologic Unit Number of 5th code watersheds Sub-Basin Name Code included in sub-basin Upper Musselshell 10040201 22 Middle Musselshell 10040202 10 Lower Musselshell 10040205 7 Box Elder 10040204 7 Flatwillow 10040203 5 Total: 51

Table 1.2. Musselshell River Assessment Units. Water Quality Use AUID Waterbody Name HUC8 County Size (miles) Category Class MUSSELSHELL RIVER, North & South MT40A001_010 Fork confluence to Deadmans Basin 10040201 WHEATLAND 5 B-2 55.3 Diversion Canal MUSSELSHELL RIVER, Deadmans MUSSELSHELL, MT40A001_020 Basin Supply Canal to HUC boundary 10040201 WHEATLAND, GOLDEN 5 C-3 94.49 near Roundup VALLEY MUSSELSHELL, MUSSELSHELL RIVER, HUC boundary 10040202 PETROLEUM, GARFIELD, 4C C-3 114.6 MT40C001_010 near Roundup to Flatwillow Creek ROSEBUD MUSSELSHELL RIVER, Flatwillow 10040205 PETROLEUM, GARFIELD 4C C-3 75.94 MT40C003_010 Creek to Fort Peck Reservoir

1.2 PROJECT OVERVIEW In 2014, the Montana Department of Environmental Quality (MDEQ) Water Quality Planning Bureau (WQPB) began a watershed planning project in the Musselshell River watershed (DEQ 2015). Among other tasks, this project involves performing water quality assessments for nutrients, metals and/or Escherichia coli (E. coli) on several waters. In 2015, monitoring activities aimed at collecting sufficient credible data to assess for nutrients, metals and E. coli will focus primarily on the mainstem Musselshell River and streams in the upper and middle sub-basins that have been selected for assessment for nutrients, metals and/or E. coli assessment. As resources allow, some monitoring may occur on several waterbodies within the lower Musselshell, Box Elder and Flatwillow sub-basins in 2015. These waters, including the lowermost Musselshell River segment, are potential candidates for nutrients, metals and/or E. coli assessment in coming years and the 2015 data collection could inform future monitoring actions if resources allow. This Sampling and Analysis Plan (SAP) presents a plan for completing monitoring associated with nutrients, metals and E. coli assessment of waters in the Musselshell River watershed during the 2015 field season.

4 WQPBMASSAP-19 1.3 ASSESSMENT SCOPE Table 1.3 shows the waterbody segments to be sampled in the Musselshell River watershed in 2015 and the nutrient and metals impairment listings that appear on the 2014 303(d) list associated with these segments (DEQ 2014). There are no existing pathogen impairment listings in the Musselshell River watershed. Table 1.4 indicates the waterbody segments which will be monitored for nutrients, metals and/or E. coli in the upper and middle Musselshell basins in 2015: 18 for nutrients, 12 for metals, and 6 for E. coli. Nutrient and metals monitoring will take place on the Musselshell River and on several tributaries. E. coli monitoring will occur on the Musselshell River and on the three reservoirs that have public recreation access points. Nine additional waterbody segments, including the lowermost segment of the Musselshell River, have been chosen for nutrient and metals monitoring in 2015, as resources allow (Table 1.5). These are segments in the lower Musselshell, Box Elder and Flatwillow basins. Although they have not been formally selected for nutrient or metals assessment, they do have proportionally greater flow and thus greater influence on the character of the Musselshell and are potential candidates for more thorough assessment in upcoming years; thus, collecting data in 2015 that aligns with assessment method data requirements will lessen the sampling burden for these streams in future years. Similarly, Lodgepole Creek is the only waterbody in the lower sub-basins which has a metals listing and is included in this document for monitoring in 2015, as resources and access allow (Table 1.5). Table 1.3. Waterbody segments in the Upper and Middle Musselshell sub-basins to be sampled in 2015 and their associated 2014 nutrients and metals 303(d) listings (DEQ 2014).

Sub- Use Metals AUID Major Waterbody Waterbody Description Nutrient Listings basin Class Listings headwaters to mouth (North Upper B-1 MT40A002_040 Mill Creek Chlorophyll-a - Fork Musselshell River) headwaters to mouth (North Upper B-1 MT40A002_030 Trail Creek Fork Musselshell River) Chlorophyll-a - North Fork Upper B-1 MT40A002_010 headwaters to Bair Reservoir Musselshell River Chlorophyll-a - Upper B-1 MT40A005_040 Bair Reservoir - - - North Fork Bair Reservoir to confluence with Upper B-1 MT40A002_010 Chlorophyll-a - Musselshell River the South Fork Musselshell River Upper - none Cottonwood Creek headwaters to mouth - - South Fork Upper - none headwaters to mouth - - Musselshell River Martinsdale Upper B-1 MT40A005_030 - - - Reservoir Upper - none Big Elk Creek headwaters to mouth - - Upper - none American Fork headwaters to mouth - - North & South Fork confluence to Nitrogen (Total), Upper B-2 MT40A001_010 Musselshell River - Deadmans Basin Diversion Canal Phosphorus (Total) Deadmans Basin Lead, Iron, Upper B-1 MT40A005_010 - - Reservoir Copper Nitrate/Nitrite (Nitrite + Nitrate as Upper C-3 MT40A002_070 Fish Creek headwaters to mouth - N), Nitrogen (Total), Phosphorus (Total)

5 WQPBMASSAP-19 Sub- Use Metals AUID Major Waterbody Waterbody Description Nutrient Listings basin Class Listings Careless Creek, headwaters to confluence with Upper - none - - upper Deadmans Basin Canal confluence with Deadmans Basin Careless Creek, Upper C-3 MT40A002_050 Canal to mouth (Musselshell - - lower River) Upper - none Big Coulee Creek headwaters to mouth - - Upper - none Painted Robe Creek headwaters to mouth Nitrogen (Total) - Nitrate/Nitrite headwaters to mouth Upper C-3 MT40A002_090 Half Breed Creek (Nitrite + Nitrate as - (Musselshell River) N), Nitrogen (Total) Deadmans Basin Supply Canal to Nitrogen (Total), Upper C-3 MT40A001_020 Musselshell River - HUC boundary near Roundup Phosphorus (Total) headwaters to mouth Nitrogen (Total), Middle C-3 MT40C002_010 North Willow Creek Iron (Musselshell River) Phosphorus (Total) HUC boundary near Roundup to Middle C-3 MT40C001_010 Musselshell River - - Flatwillow Creek

Table 1.4. Waterbody segments in the Upper and Middle Musselshell sub-basins to be sampled in 2015 and the stressor groups that will be sampled. E. AUID Major Waterbody Waterbody Description Nut. Met. coli none American Fork headwaters to mouth X - - MT40A005_040 Bair Reservoir - - - X none Big Coulee Creek headwaters to mouth X - - none Big Elk Creek headwaters to mouth X - - MT40A002_050 Careless Creek, lower confluence with Deadmans Basin Canal to mouth X X - none Careless Creek, upper headwaters to confluence with Deadmans Basin Canal X X - none Cottonwood Creek headwaters to mouth X X - MT40A005_010 Deadmans Basin Reservoir - - X X MT40A002_070 Fish Creek headwaters to mouth X - MT40A002_090 Half Breed Creek headwaters to mouth X X - MT40A005_030 Martinsdale Reservoir - - - X MT40A002_040 Mill Creek headwaters to mouth X - - MT40A001_010 Musselshell River N. & S. Fk. confluence to Deadmans Basin Diversion Canal X X X MT40A001_020 Musselshell River Deadmans Basin Supply Canal to HUC bdry. nr. Roundup X X X MT40C001_010 Musselshell River HUC boundary near Roundup to Flatwillow Creek X X X MT40A002_010 North Fork Musselshell River Bair Reservoir to confluence with S. Fk. Musselshell River X X - MT40A002_010 North Fork Musselshell River headwaters to Bair Reservoir X X - MT40C002_010 North Willow Creek headwaters to mouth X X - none Painted Robe Creek headwaters to mouth X - - none South Fork Musselshell River headwaters to mouth X X -

6 WQPBMASSAP-19 E. AUID Major Waterbody Waterbody Description Nut. Met. coli MT40A002_030 Trail Creek headwaters to mouth X - - Total: 18 12 6 Table 1.5. Waterbody segments in the Lower, Box Elder and Flatwillow sub-basins that will be sampled as resources allow in 2015 and their associated 2014 303(d) impairments (DEQ 2014). Use Nutrient Metals Sub-basin AUID Major Waterbody Nut. Met. Class Listings Listings Box Elder - none South Fork McDonald Creek - - X X Box Elder - none North Fork McDonald Creek - - X X Box Elder C-3 MT40B002_010 McDonald Creek - - X X Box Elder C-3 MT40B002_001 Box Elder Creek - - X X Flatwillow B-2 MT40B001_040 North Fork Flatwillow Creek - - X X Flatwillow B-2 MT40B001_050 South Fork Flatwillow Creek - - X X Flatwillow Creek, headwaters to Flatwillow B-2 MT40B001_021 - - X X Highway 87 bridge Flatwillow Creek, Highway 87 Nitrogen, Flatwillow B-2 MT40B001_022 Mercury X X bridge to mouth (Musselshell River) Nitrate Musselshell River, Flatwillow Creek Lower C-3 MT40C003_010 - - X X to Fort Peck Reservoir Lower C-3 MT40C004_020 Lodgepole Creek - Iron X

2.0 OBJECTIVES AND DESIGN OF INVESTIGATION

2.1 PROJECT OBJECTIVES The objectives of this project are, as follows: 1. To assess current water quality condition with respect to nutrients of those waters identified for nutrient assessment in Table 1.4 according to MDEQ nutrient assessment method (Suplee and Sada de Suplee, 2011). 2. To assess current water quality condition with respect to metals of those waters identified for metals assessment in Table 1.4 according to MDEQ metals assessment method (Drygas, 2012). 3. To assess current water quality condition with respect to E. coli of those waters identified for E. coli assessment in Table 1.4 according to Montana E. coli water quality standards (ARM 17.30.620). 4. To use the outcome of nutrient, metals and/or E. coli assessments to inform beneficial use support determinations. 5. To collect water quality data required by MDEQ assessment methods for several waters (Table 1.5) that may be assessed in upcoming years. 6. To report on current water quality condition of waters identified in Tables 1.4 and 1.5 to inform stakeholders in the Musselshell River watershed.

7 WQPBMASSAP-19 2.2 MONITORING OBJECTIVES The monitoring objectives of this project described in this SAP are, as follows:

1. To collect nutrient (TN, TP, NO 2+3) water samples from streams indicated for nutrient assessment in Table 1.4,

2. To collect ammonia (NH3+4) from the Musselshell River, particularly at sites in the vicinity of permitted facilities with nutrient discharge limits, 3. To collect up to 3 daily dissolved oxygen deltas and up to 2 periphyton samples from streams indicated for nutrient assessment in Table 1.4, 4. To collect up to 3 benthic algae (chlorophyll a and ash-free dry weight) samples from streams indicated for nutrient assessment in Table 1.4 which resemble “mountain streams” as distinguished from “prairie streams” in the MDEQ nutrient assessment method for wadeable streams: North Fork Musselshell River, South Fork Musselshell River, Cottonwood Creek, Mill Creek and Trail Creek, 5. To collect metals and hardness water samples, and at least one benthic sediment sample, on waters indicated for metals assessment in Table 1.4, 6. To collect at least 5 E. coli samples within 30-days in separate 24-hour periods (ARM 17.30.620) from waters indicated for E. coli assessment in Table 1.4, 7. To collect nutrients and metals samples on streams indicated for potential assessment in Table 1.5, and, 8. To collect TSS/TDS samples, in situ measurements of dissolved oxygen, pH, specific conductance, water and air temperature, and discharge data at all sites where water chemistry samples are collected. 2.3 SAMPLING TIMEFRAME 2.3.2 Nutrient Timeframe All nutrient data (including water chemistry, dissolved oxygen and benthic algae) will be collected between July 1, 2015 and September 30, 2015. This coincides with the allowable growing season sampling period specified in the MDEQ nutrient assessment method for wadeable streams in the Middle Rockies and Northwestern Great Plains ecoregions (Suplee and Sada de Suplee, 2011).

2.3.3 Metals Timeframe Metals water data collection efforts will take place between June 1, 2015, and October 30, 2015. Sample collection efforts may occur during high flow conditions (e.g., spring runoff or post-precipitation events), as conditions allow, and during summer and early-fall low or baseflow conditions. Benthic sediment sample collection for metals analyses will be conducted during low flow conditions (likely July 1, 2015 to September 30, 2015).

2.3.4 E. coli Timeframe E. coli monitoring will occur between July 1, 2015 and September 30, 2015. This period is within the most protective seasonal period of April 1 to October 31, as specified in the E. coli water quality standards (ARM 17.30.623 for B-1 waters, ARM 17.30.624 for B-2 waters, and ARM 17.30.628 for C-3 waters). For all waters, E. coli standards specify that a minimum of 5 samples be collected during separate 24-hour periods during any consecutive 30-day period (ARM 17.30.620).

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3.0 FIELD SAMPLING METHODS

Table 3.1 shows a list of the parameters to be sampled during field activities described in this SAP, and includes a brief description of the rationale for collecting these particular parameters, and the general timeframe during which each will be collected. 3.1 SELECTION OF SAMPLING SITES Specific site locations within the streams listed in Tables 1.4 and 1.5 were identified using GIS, topographic maps and field reconnaissance. Selected monitoring sites follow the guidelines and definitions found in the relevant assessment methods per pollutant group (e.g., nutrient sites are ≥ 1 mile apart) Suplee and Sada de Suplee, 2011; DEQ 2011a) unless a specific location is to be sampled because a potential pollutant source was identified (e.g., bracketing a point source discharge or a tributary). A complete list of the stream segments and proposed monitoring sites in the Musselshell River watershed in 2015 can be found in Appendix A. These sites are proposed locations and changes may be made based on land access or other unforeseen problems. Appendix B summarizes the sampling needs per site visit to each of these waterbodies.

3.2 PHYSICAL PARAMETERS 3.2.1 Discharge (Flow) Flow will be measured at each site during each sampling event typically using the quantitative flow meter method; the semi-quantitative float method will be used, as necessary, when high flows prevent wading (DEQ 2012b). Flow will not be measured in the field when a site is situated at or near a USGS gage station (e.g., at the mainstem Musselshell River sites).

3.2.2 Digital Photographs Digital photographs will be taken (at a minimum) at transect F of each site. At sites where benthic algae samples are collected, at least one photograph will be taken at each transect (A-K). The objective of these photos is to document visible changes in the stream flora as time passes and, as such, photos will be a combination of close-ups of stream substrate where the sampling took place and stream panoramas. The photo number and pertinent transect information will be recorded for each photo.

3.2.3 Aquatic Plant Visual Assessment Form The Aquatic Plant Visual Assessment Form will be filled out at each site visited for nutrient monitoring. This form will be completed, at most, during every nutrient sampling event and, at least, during one visit per nutrient monitoring site. Where possible, the dominant macrophytes present at the site will be identified and other observations regarding the aquatic plant community will be recorded.

3.3 WATER CHEMISTRY SAMPLING 3.3.1 In Situ Chemistry Measurements During each sampling event at each sampling site, a YSI 85 field meter will be used to collect in situ measurements of water temperature, dissolved oxygen, and specific conductance, and a portable pH meter will be used to measure pH. Air temperature will be recorded from a thermometer. These

9 WQPBMASSAP-19 measurements will be collected prior to the collection of water samples or other physical disturbances to the water column or substrate. See details about calibration in Section 6.0.

3.3.2 Unfiltered grab samples Sample containers and preservatives are summarized in Table 3.2.

(TN, TP, NO 2+3, NH3+4, TSS/TDS and total recoverable metals) For each sample, the bottle and lid will be triple-rinsed with a small amount of ambient stream water prior to grabbing the final sample. TN will be collected in a single 250ml HDPE bottle and kept on ice (not frozen) until analyzed. TP and NO2+3 (and NH3+4 on select samples) will be collected in a single 250ml HDPE bottle, will be preserved with sulfuric acid and kept on ice (not frozen) until analyzed. TSS/TDS will be collected in a single 1000 ml HDPE bottle and kept on ice (not frozen) until analyzed. Total recoverable metals will be collected in a single 250ml HDPE bottle, will be preserved with nitric acid and kept on ice (not frozen) until analyzed. Hardness will be calculated from the total recoverable metals bottle.

E. coli Detailed methodology for sample collection and analysis can be found in the E. coli Standard Operating Procedure (DEQ 2006). All E. coli water samples will be placed in new 100 ml high-density polyethylene (HDPE) bottles supplied by the laboratory. Bottles will be pre-treated with sodium thiosulfate by the laboratory; no additional preservative will be added to the sample bottle during or after sample collection, and no pre-rinsing will occur in the field. Samples will be stored on ice in a cooler at a temperature of <6 degrees Celsius (Table 3.2). E. coli samples will not be held more than six hours between collection and initiation of analysis (APHA 2005, DEQ 2006). Note: E. coli has a 6-hour holding time.

One packet of Colilert will be added to each E. coli sample (IDEXX, 2011); the sample will be gently inverted at least three times until the Colilert granules have dissolved. Each sample will be poured into a QuantiTray; each try will be sealed and labeled with the corresponding site visit code, and cut in half to fit into the incubator. Each sample will be incubated at 35 ± 0.5 degC for 24-28 hours. A blacklight will be used to read the QuantiTray E. coli results.

Total Recoverable Mercury Total recoverable mercury using the ultra-low level method follows a different sample collection procedure from other total recoverable metals samples. Samples will be collected in a 100 ml glass bottle and will be kept on ice (not frozen) until analyzed. Detailed methodology for the “clean hands/dirty hands” sample collection procedure can be found in DEQ (2012b).

Biochemical Oxygen Demand (BOD5)

Biochemical Oxygen Demand (BOD5) is a secondary (level II) parameter incorporated in MDEQ’s nutrient assessment method for wadeable prairie streams. BOD samples will only be collected if preliminary analysis of nutrient and dissolved oxygen data suggests BOD will be necessary to assess nutrient impairment. BOD5will be collected in a single 1000 ml HDPE bottle and kept on ice (not frozen) until analyzed. Note: BOD5 has a 48-hour holding time.

10 WQPBMASSAP-19 3.3.3 Filtered grab samples Dissolved Aluminum and Iron Water will be filtered through a 0.45 µm filter and 50 ml of the filtrate will be placed in a 250 ml HDPE bottle, preserved with nitric acid and kept on ice (not frozen) until analyzed (Table 3.2). Filtration will be accomplished with a 60 cm3 syringe connected to a disposal 0.45 µm filter capsule. A small amount of the sample will be wasted through the filter and the sample bottle and lid will be triple-rinsed with a small amount of filtrate before the final filtered sample is collected. Detailed methodology can be found in DEQ (2012b). Sample containers and preservatives are summarized in Table 3.2.

3.3.4 MiniDOT Dissolved Oxygen Loggers At least one MiniDOT dissolved oxygen logger will be deployed in each stream indicated for nutrient assessment in Table 1.4 (PME, 2014). Each MiniDOT will be equipped with an optical DO sensor and a coarse copper mesh anti-fouling device (Figure 3.1).

Figure 3.1. MiniDOT logger equipped with a copper mesh anti-fouling device.

Logging will be set at 15 minute intervals.

To begin deployment (PME, 2014): 1. Open the MiniDOT logger by unscrewing the white housing from the black end cap. Remove the housing completely (Figure 3.2). 2. Slide the power switch to the ON position. The LED will flash once. The MiniDOT will now delay to the start of the next minute. Observe the LED for up to 90 seconds. Sometime during this period it will flash 5 times indicating that logging has begun. If it flashes continuously, then see Section 1.2 of this manual. 3. Inspect the O-ring seal for debris. 4. Close the MiniDOT Logger by screwing the white housing back onto the black end cap. 5. Deploy the MiniDOT Logger.

MiniDOTs will be deployed horizontally, secured using zipties to a metal fencepost or length of rebar that has been secured in the stream bank. Efforts will be made to deploy the logger to maximize the flow velocity across the screen-equipped MiniDOT to avoid erroneous DO delta results (Suplee and Sada

11 WQPBMASSAP-19 de Suplee, 2013). MiniDOTs will be deployed as low in the water (i.e., as close to the streambed) as possible such that the sensor is submerged in water but elevated above and not in contact with streambed sediments.

The location, including latitude, longitude, written description and photos or a map illustration, date and time will be recorded on a datalogger deployment form for each MiniDOT deployment. Observations (written and/or photos) regarding flow conditions and the condition of the MiniDOT’s sensor underneath the coarse copper screen will be recorded during deployment and retrieval.

Wherever possible, MiniDOT loggers equipped with coarse copper mesh anti-fouling devices will be deployed for a minimum of 5 days. This is to allow for the observed “settling in” period, which may last several days or longer, during which readings may differ considerably from YSI 6600 readings, compared to later in the deployment (Suplee and Sada de Suplee, 2014). A minimum of one consecutive 24-hour deployment period is needed to be able to capture daily high and daily low concentrations and, generally, MiniDOTs will not be deployed for longer than 7 days (M. Suplee, personal communication, 5/13/15).

Where possible, efforts to clean the MiniDOT during deployment will be made; particularly in slack- water sites where algae and fine mud is more likely to build up and partially cover the optical sensor (Suplee and Sada de Suplee, 2014). MiniDOTs will be thoroughly cleaned between deployments at different sites. During cleaning, the MiniDOT will be removed and its optical sensor, copper mesh anti- fouling device and instrument body will be cleaned of biofilm, mud and any other debris encountered; water and a soft toothbrush will be used to clean gently (Suplee and Sada de Suplee, 2013).

Figure 3.2. MiniDOT circuit board and components.

To end deployment (PME, 2014):

1. Recover the miniDOT Logger 2. Clean and dry all accessible surfaces except the ‘foil’.

12 WQPBMASSAP-19 3. Open the miniDOT Logger by unscrewing the white housing from the black end cap. Remove the housing completely, taking care that water does not drip onto interior surfaces of circuits or other items inside the logger. 4. Press the File Close button. The LED should begin continuous flashing. 5. Slide the power switch to the OFF position.

Note: it is very important to press the “File Close” button to stop logging prior to turning the instrument off, or you may risk losing data.

Generally, data upload will occur in the office and not in the field. However, in the event that data must be downloaded onto a laptop computer which in the field, these steps will be followed (PME, 2014): 6. Unscrew the SD card retaining attachment. Remove the SD card by pushing down on it so the card is ejected. Use a card reader and a host computer to copy the files located in the DATA directory onto the host computer. These text files contain the measurements. (Note: SD card is not “hot swappable”. You MUST turn the power OFF before removing or reinserting the SD card. Damage may result if the power is not OFF.) 7. (Optionally) Run PME’s miniDOTPLOT.jar program to see a plot of dissolved oxygen, temperature and oxygen saturation, and to produce a concatenated file containing all the measurements.

Between deployments, the MiniDOTs will be thoroughly cleaned and dried, and will be stored in a secure location with their silica packets inside to prevent moisture damage.

3.4 BENTHIC SEDIMENT METALS Sediment metals will be passed with a minimal amount of ambient stream water through a Teflon 60- micron sieve using a Buchner funnel into a 2000 ml HDPE bottle without preservative and held on ice (not frozen) until analyzed. Detailed methodology can be found in DEQ (2012b).

3.5 BENTHIC ALGAE (CHLOROPHYLL-A AND ASH-FREE DRY WEIGHT) Benthic algae will be collected at some sites as indicated in Appendix B. Samples will be collected at 11 transects following the EMAP reach-wide procedure (Peck et al. 2006; DEQ 2011c). Samples will be collected using either the template, hoop or core methods, depending on the dominant substrate and/or algae type present. Hoop samples will be stored in zip-lock bags wrapped in aluminum foil, template samples on filters in petri dishes or centrifuge tubes wrapped in aluminum foil, and cores in centrifuge tubes wrapped in aluminum foil. All samples will be frozen (dry ice) until analyzed. Samples will be composited at the laboratory according to collection method (i.e., hoop, core, template). All samples will be frozen on dry ice (Table 3.2). Ash-free dry weight will be analyzed from the same chlorophyll a samples. Weighted averages for chlorophyll a and ash-free dry weight will be calculated, with only those transects sampled using the hoop and template methods included in the ash-free dry weight weighted average.

13 WQPBMASSAP-19 3.6 PERIPHYTON Periphyton samples will be collected at sites as indicated in Appendix B. Periphyton will be collected with the PERI-1 method, which is appropriate for assessing non-flowing streams where the collector may be restricted to extant pools, and is also appropriate for flowing streams where a defined reach is not being established (DEQ 2011c). The sample is a single composite sample representing a miniature replica of the stand of algae which are present within a stream reach about 50 m up- and downstream from the initial arrival site (~100 m total), to assure that the collection area is fairly typical of the site in question.

Microalgae will be collected from natural substrates in proportion to the approximate rank of those substrates at the study site. Collection involves scraping the entire upper surface of several rocks (small gravel through cobbles), lifting the algal film off of near-shore sediments, scraping submerged branches, and sucking up fine sediment in depositional areas. Macroalgae are picked by hand in proportion to their abundance at the site, and the sampler will attempt to visually distinguish between the various growth forms that represent different algal taxa.

All collections of microalgae and macroalgae will be composited in a 50 cm3 centrifuge tube. In the field, enough ambient water will be added to cover the collected material and achieve a volume of 45-48 ml. The sample will be preserved with formalin (i.e., 40% formaldehyde solution) to bring the final sample solution strength to about 2-4%. This equates to adding about 2-5 ml of formalin to the sample centrifuge tube. Samples will be stored without refrigeration in a dark place (i.e., inside a cooler) during transport until delivery to Rhithron Laboratory.

Table 3.1. Water Quality Parameters to be Sampled in the Musselshell River Watershed and the Rationale and Timing for Sample Collection. High Flow Low Flow Parameter Group Rationale (May, (July - June) Sept) DEQ's nutrient assessment method is based on Montana's numeric WQS for total nitrogen (TN) ang total phosphorus (TP). NO helps to identify Nutrients (TP, TN, 2+3 potential nitrogen impairment issues which are not - X NO2+3, NH3+4) detected via TN. NH3+4 is to be sampled where permitted discharge limits for ammonia exists (e.g., near wastewater treatment facilities). Total Recoverable Total recoverable metals include dissolved and Metals bioavailable metals in ambient water and are used to X X (As, Cd, Cr, Cu, Fe, Pb, apply Montana’s numeric WQS. Se, Ag, Zn, Hg) Dissolved metals are used to help interpret the source, transport and toxicity of metals. The water quality standard for dissolved aluminum is for the dissolved Dissolved metals (Al, Fe) X X fraction. Areas with naturally-high levels of iron may be common in the Musselshell watershed and dissolved iron will assist with source identification.

14 WQPBMASSAP-19 High Flow Low Flow Parameter Group Rationale (May, (July - June) Sept) Common cations generally reflect natural geology. Ca Common Cations (Ca, and Mg are used to calculate hardness which is X X Mg) necessary to calculate several metals' WQS which are hardness-dependent. E. coli is a bacteria which can be indicative of Escherichia coli (E. coli) contamination of water by fecal matter, and can lead - X to illness and limit recreational use of water. Total Suspended Solids TSS is used to help understand NPS erosion and X X (TSS) sources of pollutants. TDS is the total weight of all solids dissolved in a given Total Dissolved Solids volume of water and may be used as an indicator of X X (TDS) pollutants and salinity.

BOD is the amount of dissolved oxygen needed by aerobic biological organisms in a body of water to Biochemical Oxygen break down organic material present in a given water - X Demand (BOD5) sample at certain temperature over a specific time period. BOD is a secondary parameter included in the nutrient assessment method for prairie streams.

Sediment metals data are used to help identify the Sediment Metals source and transport of metals, which may remobilize - X in the water column under certain conditions. Elevated daily DO delta values indicate high Dissolved Oxygen Delta productivity and the potential for DO standards - X (Daily Max - Daily Min) exceedances (per DEQ-7) that would impact fish and aquatic life. Benthic algae is a primary parameter for nutrient Benthic Algae assessment in wadeable mountain streams. Algae will (chlorophyll a and ash- be collected from select streams in the upper - X free dry weight) Musselshell basin which originate in the Middle Rockies ecoregion to inform nutrient assessment. Measures of the structure of algal associations, such as species diversity and dominance, can be sensitive Periphyton and useful indicators of water-quality impacts and - X ecological disturbance. Periphyton is a parameter required by the nutrient assessment method. Discharge (flow) allows for the calculation of pollutant Discharge X X loads (load = concentration x flow)

15 WQPBMASSAP-19

Table 3.2. Sample Volume, Container, Preservation and Storage, and Holding Time per Analyte. Preservation and Analyte Bottle Size Container Holding Time Storage TPN 250 ml HDPE Bottle Cool to <6 °C (on ice) 28 days H₂SO₄; Cool to <6 °C TP, NO , NH 500 ml HDPE Bottle 28 days 2+3 3+4 (on ice) pre-treated by IDEXX Collection laboratory with sodium Escherichia coli 100 ml 6 hours bottles thiosulfate; Cool to <6°C (on ice)

TSS and TDS 1000 ml HDPE Bottle Cool to <6 °C (on ice) 7 days

Biochemical Oxygen 1000ml HDPE Bottle Cool to <6 °C (on ice) 48 hrs Demand (BOD5) Total Recoverable Metals 5 ml conc. HNO , cool 250 ml HDPE Bottle 3 180 days (including hardness) to <6°C (on ice) Field filter 0.45 um, 1.5 Dissolved Metals 250 ml HDPE Bottle ml conc. HNO3, cool to 180 days <6°C (on ice) 0.5 ml 12N HCl, Cool to Ultra Low Level Mercury 100 ml Glass Bottle 28 days <6 °C (on ice)

Sediment Metals 2000 ml HDPE Bottle Cool to <6 °C (on ice) 180 days

50 mL centrifuge Benthic Algae Freeze tube (template (chlorophyll a and ash- - (on dry ice); 45 days or core), ziplock free dry weight) <0°C bag (hoop) Formalin (40% 50 mL centrifuge Periphyton 50 mL formaldehyde n/a tube solution); No ice

4.0 SAMPLE HANDLING PROCEDURES

This project will follow WQPB’s “internal process” for sample handling. Each sample will be labeled with the appropriate label and all samples and associated field forms will be identified using unique Site Visit Codes. Appropriate storage times for water quality samples are discussed in Section 3 and shown in Table 3.2. Upon return from the field, all samples collected with be delivered to the Monitoring and

16 WQPBMASSAP-19 Assessment Resource Storage facility (i.e., “The Watershed”) on Carter Drive, Helena, MT. Samples will remain accompanied by their Site Visit/Chain of Custody form at all times, and appropriate signatures will be included to identify chain of custody upon relinquishing samples. Following an initial QC check of field forms and samples, water chemistry, sediment metals and benthic algae samples will be delivered to Energy Laboratory and periphyton samples will be delivered to Rhithron, Inc. for analysis. 5.0 LABORATORY ANALYTICAL MEASUREMENTS

Table 5.1 summarizes, per analyte, the analytical methods and detection/reporting limits to be used for this project during field season 2015.

Table 5.1 – Analytical Methods and Required Reporting Values Preferred Req. Report Limit Parameter Alternate Method Method ug/L Water Sample - Common Ions, Physical Parameters, Miscellaneous Total Suspended Solids (TSS) A2540 D 4000

Total Dissolved Solids (TDS) A2540 C 4000

E. Coli* A9223 B EPA 160.4 1 MPN/100 ml Biochemical Oxygen Demand A5210 B 4000 (BOD) Water Sample - Nutrients

Total Persulfate Nitrogen (TPN) A4500-N C A4500-N B 40 Total Phosphorus as P EPA 365.1 A4500-P F 3 Nitrate-Nitrite as N EPA 353.2 A4500-NO3 F 10 A4500-NH3 Total Ammonia as N EPA 350.1 50 B,C,D,E,or G Water Sample - Dissolved Metals (0.45 um filtered) Aluminum EPA 200.7 EPA 200.8 9 Iron EPA 200.7 20

Water Sample - Total Recoverable Metals

Total Recoverable Metals EPA 200.2 APHA3030F (b) N/A Digestion Arsenic EPA 200.8 1

Cadmium EPA 200.8 0.03

Chromium EPA 200.8 EPA 200.7 1 Copper EPA 200.8 EPA 200.7 1 Iron EPA 200.7 20

Lead EPA 200.8 0.3

Selenium EPA 200.8 1

Silver EPA 200.8 EPA 200.7/200.9 0.2 Zinc EPA 200.7 EPA 200.8 8 Total Hardness as CaCO A2340 B (Calc) 1000 3 Water Sample - Total Mercury, Ultra low level EPA 245.7 0.005

17 WQPBMASSAP-19 Preferred Req. Report Limit Parameter Alternate Method Method ug/L Sediment Sample - Total Recoverable Metals Total Recoverable Metals EPA 200.2 N/A Digestion Arsenic EPA 200.8 EPA 200.9 1 Cadmium EPA 200.8 EPA 200.9 0.2 Chromium EPA 200.8 EPA 200.7 9 Copper EPA 200.8 EPA 200.7 15 Iron EPA 200.7 EPA 200.7 10 Lead EPA 200.8 EPA 200.9 5 Zinc EPA 200.7 EPA 200.7 20 Sediment Sample - Total Metals Mercury EPA 7471B 0.05

Preferred Req. Report Limit Parameter Alternate Method Method mg/m2 Substrate Sample - Chlorophyll-a Chlorophyll-a A 10200 H n/a

Ash Free Dry Weight (AFDW) A 10300 C (5) n/a

*Note: DEQ staff will be performing incubation and analysis in the field for all E. coli samples using the Colilert QuantiTray method.

6.0 QUALITY ASSURANCE AND QUALITY CONTROL REQUIREMENTS

This project will follow the WQPB “internal process” for quality assurance and quality control; all QA/QC requirements followed by MT DEQ will be instituted for this project. The QA/QC requirements are described in DEQ (2005).

6.1 INSTRUMENT CALIBRATION 6.1.1 YSI 85 Handheld Meter Pre-calibration of the YSI 85 meter will be undertaken in the laboratory. The YSI meter will also be calibrated in the field just prior to measuring dissolved oxygen for site-specific altitude at each site following the instructions in the YSI 85 operations manual.

6.1.2 Handheld pH Meter The pH meter will be pre-calibrated in the laboratory using the two-point method (pH 4.0 and 7.0 standards) prior to first use during the 2015 field season. The meter will be checked against a 4.0 and 7.0 standard and recalibrated, as needed and according to the instrument’s operations manual, prior to each sampling trip.

6.1.3 MiniDOT Dissolved Oxygen Dataloggers MiniDOTs come pre-calibrated and ready to use from the company (PME, 2014). Refer to Section 3.3.4 for details regarding cleaning and maintenance of these instruments.

18 WQPBMASSAP-19

6.2 FIELD DUPLICATES Sampling locations where duplicates are collected will be randomly selected. Sample duplicates will be collected for each water chemistry sample and for benthic sediments on at least 10% of the total samples for each parameter. All collection, preservation, storage and handling methods used to collect the routine sample will be followed when collecting duplicate samples.

6.3 FIELD BLANKS Field blanks will be prepared prior to departure from the field at the end of each sampling run (“trip”) for each of the water chemistry analytes collected during that trip. Trip blanks, supplied by Energy Lab, will be prepared for ultra low-level mercury. Field blanks will be prepared using deionized water supplied from the lab.

7.0 DATA ANALYSIS, RECORD KEEPING, AND REPORTING REQUIREMENTS

This project will follow the WQPB “internal process” for record keeping and reporting. Site Visit/Chain of Custody forms, field forms, digital photos, and laboratory results will be processed by WQPB staff following QA/QC procedures as indicated in Section 6.0.

7.1 NUTRIENT ASSESSMENT

TN, TP, NO 2+3, benthic algae, dissolved oxygen deltas, and periphyton (and BOD5, if collected) data will be analyzed as specified in MDEQ’s nutrient assessment method for wadeable streams (Suplee and Sada de Suplee, 2011). TN and TP data will be evaluated against Montana’s numeric water quality standards for TN and TP (DEQ 2014). NO2+3 data will be evaluated against recommended screening criteria of 1.0 mg/L.

NH3+4 data will be analyzed against Montana’s numeric ammonia samples, accompanied by pH and temperature data recorded at the time of collection, as specified in DEQ-7 (DEQ 2012a).

7.2 METALS ASSESSMENT Metals data will be evaluated against Montana’s numeric water quality standards for metals (DEQ 2012a) according to the MDEQ metals assessment method (Drygas, 2012). Sediment metals data will be evaluated against benthic sediment screening criteria endorsed by the National Oceanic and Atmospheric Administration (NOAA) (Buchman, 2008). Benthic sediment metals will be used to identify presence of metals and potential for sediment entrainment to inform metals assessment, but is not directly incorporated during assessment of metals impairment.

7.3 E. COLI ASSESSMENT E. coli data will be used to assess E. coli impairment status according to specifications included in Montana’s water quality standards, which read:

19 WQPBMASSAP-19 For B-1, B-2 and C-3 streams: “from April 1 through October 31, the geometric mean number of E-coli may not exceed 126 colony forming units per 100 milliliters and 10% of the total samples may not exceed 252 colony forming units per 100 milliliters during any 30-day period” (ARM 17.30.623, 624 and 628, respectively). For each waterbody segment being assessed, data will be grouped both by segment and by site; percent exceedance rate and geometric mean will be calculated for both groupings and compared against water quality standards to determine impairment.

7.4 SOURCE IDENTIFICATION 7.4.1 Permitted Facilities The locations of permitted point source discharges were identified using state records (via GIS) during risk assessment and site selection. Water chemistry samples will be collected, where possible, both upstream and downstream from permitted point source discharges to help facilitate the calculation of discharge limits, point source loading and background concentrations. Samples collected outside of the allowable mixing zone and on the segment being assessed will be incorporated into assessment determinations. Samples collected from upstream and downstream of discharges will be considered independent of one another even if < 1 mile apart, as specified in the nutrient and metals assessment methods (cite).

7.4.2 Irrigation Ditches Several large irrigation ditches were identified during risk assessment and site selection (Appendix A). Water chemistry samples will be collected from select irrigation canals and ditches throughout the project area, as access permits. Generally, sites will be situated near the return flow of the canal or ditch, near where water re-enters the waterbody segment being assessed. This data will be used to help facilitate source identification and loading calculation. Data collected from canals/ditches will not be included in assessment determinations for waterbody segments as the data is not being collected from the assessment unit itself.

8.0 SCHEDULE

The Water Quality Monitoring and Assessment staff will sample 21 waterbody segments within the upper and middle Musselshell River basins, and up to 10 additional waterbody segments within the Box Elder, Flatwillow and lower Musselshell basins. Proposed monitoring sites on each waterbody are shown in Appendix A. The high flow sampling events (for metals) will occur in June 2015 whereas the low flow sampling events (for metals, nutrients and E. coli) will occur in July, August and September, 2015. Nutrient data collection should be completed no later than September 30, 2015 (Suplee and Sada de Suplee, 2011), and low flow monitoring for metals or E. coli may extend up to October 31, 2015.

9.0 PROJECT TEAM AND RESPONSIBILITIES

The Water Quality Monitoring and Assessment Section will lead the monitoring component. Darrin Kron will oversee the overall Monitoring and Assessment component. Kathryn Makarowski will lead the nutrients, E. coli and metals monitoring project. Randy Apfelbeck, Steve Fernandes, Jess Clarke, Al Nixon, Steve Edmonds and Lou Volpe will provide monitoring support in the field. Montana Tech student contractors, Meg Doolittle and Jay Hillygus, may also provide field sampling support. Jordan Tollefson

20 WQPBMASSAP-19 will assist with stakeholder outreach and other outreach-oriented aspects of project coordination. Dean Yashan will oversee the overall TMDL component.

10.0 REFERENCES

APHA (American Public Health Association). 2005. Standard methods for the examination of water and wastewater. 21st ed. American Public Health Association, American Water Works Association, and Water Pollution Control Federation. Washington, DC.

Buchman, Michael F. 2008. NOAA Screening Quick Reference Tables. Seattle, WA: NOAA. NOAA HAZMAT Report 08-1. http://response.restoration.noaa.gov/book_shelf/122_NEW-SQuiRTs.pdf.

DEQ (Montana Department of Environmental Quality). 2005. Quality Assurance Project Plan (QAPP) Sampling and Water Quality Assessment of Streams and Rivers in Montana, 2005. Available at: http://www.deq.state.mt.us/wqinfo/QAProgram/WQPBQAP-02.pdf. DEQ (Montana Department of Environmental Quality). 2006. Sample Collection, Handling and Analysis of Escherichia Coli. Water Quality Planning Bureau. WQPBWQM-014. Available at: http://deq.mt.gov/wqinfo/qaprogram/sops.mcpx. DEQ (Montana Department of Environmental Quality). 2011a. Water Quality Assessment Method. Helena, MT: Montana Department of Environmental Quality.

DEQ (Montana Department of Environmental Quality). 2011b. Sample Collection and Laboratory Analysis of Chlorophyll a Standard Operating Procedure. Prepared by Suplee, M., February 2011. Document No. WQPBWQM-011, Revision 5.

DEQ (Montana Department of Environmental Quality). 2011c. Periphyton Standard Operating Procedure. Document No. WQPBWQM-010, v2. Available at: http://deq.mt.gov/wqinfo/qaprogram/sops.mcpx.

DEQ (Montana Department of Environmental Quality). 2012a. Circular DEQ-7: Montana Numeric Water Quality Standards. Helena, MT: Montana Department of Environmental Quality. http://deq.mt.gov/wqinfo/Circulars.mcpx. Accessed 1/15/2013a.DEQ (Montana Department of Environmental Quality). 2014. Montana 2014 Final Water Quality Integrated Report. Helena, MT: Montana Dept. of Environmental Quality.

DEQ (Montana Department of Environmental Quality). 2012b. Water Quality Planning Bureau Field Procedures Manual For Water Quality Assessment Monitoring Version 3.0. Helena, MT: Montana Dept. of Environmental Quality.

DEQ (Montana Department of Environmental Quality). 2014. Department Circular DEQ-12A: Montana Base Numeric Nutrient Standards. Helena, MT: Montana Dept. of Environmental Quality. Available at http://deq.mt.gov/wqinfo/Standards/default.mcpx.

DEQ (Montana Department of Environmental Quality). 2015. DRAFT Musselshell Water Quality Project Plan. LMO-TMDL-01.

21 WQPBMASSAP-19 Drygas, Jonathan. 2012. The Montana Department of Environmental Quality Metals Assessment Method. Helena, MT: Montana Dept. of Environmental Quality.

IDEXX, Inc. 2011. How to Use Colilert. [Available at http://www.idexx.com/water/colilert/index.jsp]. Accessed on May 17, 2011.

Precision Measurement Engineering, Inc. (PME). 2014. MiniDO2T User’s Manual, 2014. Precision Measurement Engineering, Inc. Available at www.pme.com.

Suplee, M.W., and R. Sada de Suplee. 2011. Assessment Methodology for Determining Wadeable Stream Impairment Due to Excess Nitrogen and Phosphorus Levels. Helena, MT: Montana Dept. of Environmental Quality.

Suplee, M. and R. Sada de Suplee. 2013. Technical Memorandum: Best use of MiniDOT Loggers for Dissolved Oxygen Measurement in Streams and Rivers, Part 2. DEQ (Montana Department of Environmental Quality). May 13, 2013.

Suplee, M. and R. Sada de Suplee. 2014. Technical Memorandum: Best use of MiniDOT Loggers for Dissolved Oxygen Measurement in Streams and Rivers, Part 2. DEQ (Montana Department of Environmental Quality). November 14, 2014.

22 WQPBMASSAP-19 APPENDIX A – PROPOSED MONITORING LOCATIONS AND SITE RATIONALE

AUID County Waterbody Name Latitude Longitude Site Description Site Rationale

- WHEATLAND American Fork 46.2870 -109.8835 Above Hwy 191 Assessment - WHEATLAND American Fork 46.3732 -109.7963 near mouth, above Lebo Creek, at Red Bridge Rd Assessment - WHEATLAND Lebo Creek 46.3807 -109.7996 near mouth, at Red Bridge Rd Source Identification - WHEATLAND American Fork 46.3923 -109.7580 near mouth, at Five Mile Loop below Lebo Cr. Assessment MT40A005_040 MEAGHER Bair Reservoir 46.5788 -110.5594 near Dam Assessment MT40A005_040 MEAGHER Bair Reservoir 46.5817 -110.5675 at boat ramp, fishing access Assessment - STILLWATER Big Coulee Creek 46.1192 -109.3759 above gas wells Assessment - GOLDEN VALLEY Big Coulee Creek 46.1550 -109.2768 below N. and S. Fk. confluence; below Big Coulee Rd (Hwy 300) Assessment - GOLDEN VALLEY Big Coulee Creek 46.2380 -109.1369 above Harms Rd Assessment - GOLDEN VALLEY Big Coulee Creek 46.2556 -109.0525 at Cushman Rd Assessment - GOLDEN VALLEY Big Coulee Creek 46.2803 -108.9390 near mouth, at Hwy 3 Assessment - WHEATLAND Big Elk Creek 46.2749 -110.1773 near headwaters Assessment - WHEATLAND Big Elk Creek 46.3289 -110.0360 at Porcupine Rd Assessment - WHEATLAND Big Elk Creek 46.4153 -110.0665 near mouth Assessment - Wheatland Big Elk Creek 46.3201 -110.0900 at Big Elk Rd Assessment - WHEATLAND Big Elk Creek 46.4254 -110.0596 near mouth Assessment MT40B002_001 PETROLEUM Box Elder Creek 46.9658 -108.0845 near mouth Assessment - WHEATLAND Careless Creek, upper 46.6126 -109.4729 near headwaters at Hwy 297 and Judith Rd Assessment - WHEATLAND Careless Creek, upper 46.4606 -109.4870 at Hwy 297/Hedgeville Rd Assessment - Roberts Creek 46.4535 -109.5030 at Hwy 297 (d/s of bridge) Source Identification

- GOLDEN VALLEY Careless Creek, upper 46.4075 -109.3792 b/w Roberts Cr. and Swimming Woman Cr. above Wallum Rd Assessment - GOLDEN VALLEY Careless Creek, upper 46.3778 -109.2906 u/s from Deadmans Basin, below Swimming Woman Creek Assessment - GOLDEN VALLEY Deadman's Basin Outlet Canal 46.3718 -109.2923 near mouth below spillway Source Identification MT40A002_050 GOLDEN VALLEY Careless Creek, lower 46.3736 -109.2854 d/s from Deadmans Basin; below Jessie Rd. Assessment MT40A002_050 GOLDEN VALLEY Careless Creek, lower 46.3631 -109.2684 at Hwy 238 Bridge Assessment MT40A002_050 GOLDEN VALLEY Careless Creek, lower 46.3554 -109.2430 at bridge past house and office on Quad Five Ranch Assessment MT40A002_050 GOLDEN VALLEY Careless Creek, lower 46.3349 -109.2169 north of Sterling Rd. Assessment MT40A002_050 GOLDEN VALLEY Careless Creek, lower 46.3302 -109.1980 at Sterling Rd./Ninemile Rd. Assessment MT40A002_050 GOLDEN VALLEY Careless Creek, lower 46.3164 -109.1869 near mouth at Hwy 12 Assessment - MEAGHER Cottonwood Creek 46.3111 -110.4185 near headwaters Assessment - MEAGHER Cottonwood Creek 46.3376 -110.4050 at Cottonwood Cr Rd Assessment - MEAGHER Cottonwood Creek 46.3840 -110.3933 mid-segment, below Lost Horse Cr. Assessment - MEAGHER Cottonwood Creek 46.4336 -110.4070 near mouth and Hwy 294 crossing Assessment MT40A005_010 WHEATLAND Deadmans Basin Reservoir 46.3280 -109.4288 at Bluebird Campground Assessment MT40A005_010 WHEATLAND Deadmans Basin Reservoir 46.3346 -109.4440 at Breezy Point, near Ditch inlet Assessment - ROSEBUD Delphia/Melstone South Canal 46.7249 -107.8269 above Musselshell confluence Source Identification

23 WQPBMASSAP-19 AUID County Waterbody Name Latitude Longitude Site Description Site Rationale

MT40A002_070 SWEET GRASS Fish Creek 46.2024 -110.0026 near headwaters Assessment MT40A002_070 WHEATLAND Fish Creek 46.2321 -109.8955 at Hwy 191 Assessment MT40A002_070 WHEATLAND Fish Creek 46.2525 -109.7688 below Hwy 191 Assessment MT40A002_070 GOLDEN VALLEY Fish Creek 46.2118 -109.5899 below S Shamut Rd Assessment MT40A002_070 WHEATLAND Fish Creek 46.2334 -109.4578 below Simmons Creek Assessment MT40A002_070 GOLDEN VALLEY Fish Creek 46.2794 -109.2580 at Hwy 300 S of Ryegate Assessment MT40B001_022 PETROLEUM Flatwillow Creek 46.9558 -108.0747 above Boxelder Creek Assessment MT40A002_090 MUSSELSHELL Half Breed Creek 46.2703 -108.4706 near headwaters, above Rehder Creek Assessment MT40A002_090 MUSSELSHELL Half Breed Creek 46.3175 -108.4884 below Rehder Creek Assessment MT40A002_090 MUSSELSHELL Half Breed Creek 46.3622 -108.4977 at Hwy 87 Assessment MT40A002_090 MUSSELSHELL Half Breed Creek 46.3872 -108.5418 at USGS site Half Breed Creek near Klein MT Assessment MT40A002_090 MUSSELSHELL Half Breed Creek 46.4175 -108.5610 1 mile u/s from mouth Assessment MT40A005_030 WHEATLAND Martinsdale Reservoir 46.4501 -110.2772 nearest to fishing access facility Assessment MT40A005_030 WHEATLAND Martinsdale Reservoir 46.4519 -110.2717 near Dam Assessment MT40A002_040 MEAGHER Mill Creek 46.6629 -110.5598 0.5 mile above Jamison Tr (FR47 or 2019?) crossing Assessment MT40A002_040 MEAGHER Mill Creek 46.6544 -110.5621 0.5 mile below Jamison Tr (FR47 or 2019?) crossing Assessment MT40A002_040 MEAGHER Mill Creek 46.6422 -110.5729 near mouth Assessment MT40A001_020 GOLDEN VALLEY Musselshell River, MT40A001_020 46.2891 -108.9380 above Big Coulee Creek at Lavina; At USGS Site 06125600 Assessment MT40A001_020 GOLDEN VALLEY Musselshell River, MT40A001_020 46.2939 -109.2581 Above Rygate; South of Hwy 12 on Rt 300, at Ryegate Bridge Assessment MT40A001_020 GOLDEN VALLEY Musselshell River, MT40A001_020 46.3084 -109.1914 b/w Fish Cr. and Careless Cr., at USGS site 06123500 Assessment MT40A001_020 WHEATLAND Musselshell River, MT40A001_020 46.3190 -109.4610 above Mud Cr nr Shawmut; At USGS site 06123030 Assessment MT40A001_020 MUSSELSHELL Musselshell River, MT40A001_020 46.3483 -108.6758 above Currant and Goulding Creeks Assessment MT40A001_010 WHEATLAND Musselshell River, MT40A001_010 46.4088 -109.6981 below American Fork Assessment MT40A001_010 WHEATLAND Musselshell River, MT40A001_010 46.4169 -109.7776 below Antelope Creek and Harlow WWTP discharge, above American Fork Assessment MT40A001_020 MUSSELSHELL Musselshell River, MT40A001_020 46.4277 -108.5746 between Horsethief and Halfbreed Assessment MT40C001_010 MUSSELSHELL Musselshell River, MT40C001_010 46.4280 -108.5730 near Roundup; At USGS site 06126500 Assessment MT40A001_010 WHEATLAND Musselshell River, MT40A001_010 46.4289 -109.8410 at Harlowton, At USGS site 06120500 Assessment MT40A001_010 WHEATLAND Musselshell River, MT40A001_010 46.4301 -110.0722 near Two Dot Assessment MT40A001_010 WHEATLAND Musselshell River, MT40A001_010 46.4301 -110.0722 near Two Dot Assessment MT40C001_010 MUSSELSHELL Musselshell River, MT40C001_010 46.4391 -108.5370 above Roundup WWTP Assessment MT40C001_010 MUSSELSHELL Musselshell River, MT40C001_010 46.4528 -108.4796 below Roundup WWTP and above Willow Cr. Assessment MT40A001_010 WHEATLAND Musselshell River, MT40A001_010 46.4636 -110.2264 Selkirk FAS Assessment MT40A001_010 WHEATLAND Musselshell River, MT40A001_010 46.4770 -110.2490 near Martinsdale; AT USGS site 06119600, below Martinsdale Colony Assessment MT40C001_010 MUSSELSHELL Musselshell River, MT40C001_010 46.5224 -108.1090 at Musselshell; At USGS Site 06127500, off of Hwy 12 Assessment MT40C001_010 MUSSELSHELL Musselshell River, MT40C001_010 46.6213 -107.8218 above Home Creek, below Hwy 12 crossing Assessment MT40C001_010 ROSEBUD Musselshell River, MT40C001_010 46.7199 -107.8222 at Bridge Rd bridge Assessment MT40C001_010 PETROLEUM Musselshell River, MT40C001_010 46.8494 -107.8925 above North Willow Cr. confluence Assessment MT40C001_010 PETROLEUM Musselshell River, MT40C001_010 46.9225 -107.9199 above Flatwillow Creek, at USGS Site 06127600 near Mosby Assessment

24 WQPBMASSAP-19 AUID County Waterbody Name Latitude Longitude Site Description Site Rationale

MT40C003_010 PETROLEUM Musselshell River, MT40C003_010 46.9947 -107.8890 at Mosby MT; At USGS site 06130500 Assessment MT40C003_010 GARFIELD Musselshell River, MT40C003_010 47.1674 -107.9473 above Calf Creek Assessment MT40C003_010 GARFIELD Musselshell River, MT40C003_010 47.2103 -107.9353 below Calf Creek Assessment MT40C003_010 PETROLEUM Musselshell River, MT40C003_010 47.2818 -107.9611 below Dovetail along 79 Trail Assessment MT40C003_010 GARFIELD Musselshell River, MT40C003_010 47.3097 -107.9556 near mouth Assessment MT40A002_010 MEAGHER North Fork Musselshell River, upper 46.6602 -110.5925 near headwaters, below wetland complex where more likely to be water Assessment MT40A002_010 MEAGHER North Fork Musselshell River, upper 46.7102 -110.5967 near headwaters (power line intersection due west is at -110.601831 46.709365) Assessment MT40A002_010 MEAGHER North Fork Musselshell River, upper 46.6401 -110.5736 near ranch road crossing below Mill Creek Assessment MT40A002_010 MEAGHER North Fork Musselshell River, upper 46.6145 -110.5770 near ranch road crossing above Bair Reservoir and below Copper Cr Assessment MT40A002_010 MEAGHER North Fork Musselshell River, lower 46.5751 -110.5539 below Bair Reservoir Assessment MT40A002_010 MEAGHER North Fork Musselshell River, lower 46.5728 -110.5366 Below Checkerboard and Brooks Creek confluence Assessment MT40A002_010 MEAGHER North Fork Musselshell River, lower 46.5628 -110.5107 below Trail Creek Assessment MT40A002_010 MEAGHER North Fork Musselshell River, lower 46.5417 -110.4429 near Spring Creek confluence Assessment MT40A002_010 MEAGHER North Fork Musselshell River, lower 46.4866 -110.3010 near mouth, off of Hwy 12 north of Martinsdale Assessment MT40A002_010 WHEATLAND North Fork Musselshell River, lower 46.4815 -110.2750 just above mouth Assessment MT40C002_010 MUSSELSHELL North Willow Creek 46.7212 -107.9994 Off of Ragged Point Rd Assessment MT40C002_010 MUSSELSHELL North Willow Creek 46.6007 -108.4127 300 yards d/s from Griffith Road Assessment MT40C002_010 MUSSELSHELL North Willow Creek 46.6254 -108.3037 above Little Wall, at N Gage Rd Assessment MT40C002_010 MUSSELSHELL North Willow Creek 46.6663 -108.1550 below Little Wall; at Fourmile Rd ~16 miles N of Musselshell Assessment MT40C002_010 PETROLEUM North Willow Creek 46.8433 -107.9000 At hwy 500 (Melstone Rd) Assessment - STILLWATER Painted Robe Creek 46.0915 -109.2004 uppermost site near headwaters Assessment - STILLWATER Painted Robe Creek 46.1307 -109.0862 mid-segment Assessment - GOLDEN VALLEY Painted Robe Creek 46.1861 -108.9889 at lower crossing on Painted Road Road Assessment - GOLDEN VALLEY Painted Robe Creek 46.2090 -108.9227 at Buffalo Road and Hwy 3 Assessment - GOLDEN VALLEY Painted Robe Creek 46.2702 -108.8225 at Three Pines Road crossing near mouth Assessment - FERGUS South Fork McDonald Creek 47.0409 -108.8120 at Hwy 87/200 Assessment - MEAGHER South Fork Musselshell River 46.3733 -110.6250 near headwaters at Bruno below confluence of Dry Fork and Warm Springs Assessment - MEAGHER South Fork Musselshell River 46.4132 -110.5437 at Lennep Rd crossing Assessment - MEAGHER South Fork Musselshell River 46.4533 -110.3820 at USGS site 06118500, (71 Ranch LP) off MT 294 Assessment - MEAGHER South Fork Musselshell River 46.4531 -110.3557 off Martinsdale Rd. near mouth Assessment - MEAGHER South Fork Musselshell River 46.4660 -110.3171 at Route 294 bridge near mouth Assessment - WHEATLAND South Fork Musselshell River 46.4792 -110.2754 near mouth, downstream from USGS gage Assessment MT40A002_030 MEAGHER Trail Creek 46.6726 -110.5115 near headwaters, above mining influence Assessment MT40A002_030 MEAGHER Trail Creek 46.6443 -110.5177 Below headwaters, at NFD2019 Assessment MT40A002_030 MEAGHER Trail Creek 46.6046 -110.5181 mid-segment Assessment MT40A002_030 MEAGHER Trail Creek 46.5645 -110.5123 near mouth Assessment

25 WQPBMASSAP-19

APPENDIX B – PROPOSED MONITORING LOCATIONS AND SAMPLING NEEDS PER SITE

TN, Benthic E. Waterbody Name Latitude Longitude Site Description Owner(s) TP, NH DO Peri Metals 3+4 algae coli NO2+3 American Fork 46.2870 -109.8835 Above Hwy 191 TwoDot Land & Livestock Co X X X X American Fork 46.3732 -109.7963 near mouth, above Lebo Creek, at Red Bridge Rd Elk Ridge Limited Partnership X X X X Lebo Creek 46.3807 -109.7996 near mouth, at Red Bridge Rd Elk Ridge Limited Partnership X X American Fork 46.3923 -109.7580 near mouth, at Five Mile Loop below Lebo Cr. Elk Ridge Limited Partnership X X X X Bair Reservoir 46.5788 -110.5594 near Dam FWP X

Bair Reservoir 46.5817 -110.5675 at boat ramp, fishing access FWP X

Big Coulee Creek 46.1192 -109.3759 above gas wells THREE CROSS RANCH LLC X X Big Coulee Creek 46.1550 -109.2768 below N. and S. Fk. confluence; below Big Coulee Rd (Hwy 300) Montana State Trust Lands; State of Montana X X X X Big Coulee Creek 46.2380 -109.1369 above Harms Rd Montana State Trust Lands; State of Montana X X Big Coulee Creek 46.2556 -109.0525 at Cushman Rd Bryant, Michael R and Jacqueline X X Big Coulee Creek 46.2803 -108.9390 near mouth, at Hwy 3 Bilden, Dan A X X X X Big Elk Creek 46.2749 -110.1773 near headwaters McFarland-White Ranch X X X Big Elk Creek 46.3289 -110.0360 at Porcupine Rd C Bar J Ranch LLC X Big Elk Creek 46.4153 -110.0665 mid-segment McFarland-White Ranch X Big Elk Creek 46.3201 -110.0900 at Big Elk Rd M AND W RANCH LIMITED PARTNERSHIP X Big Elk Creek 46.4254 -110.0596 near mouth McFarland-White Ranch X X X Box Elder Creek 46.9658 -108.0845 near mouth Twin Creek Farm and Ranch, LLC X X Careless Creek, upper 46.6126 -109.4729 near headwaters at Hwy 297 and Judith Rd Thomas Glennie Estate X X X X Careless Creek, upper 46.4606 -109.4870 at Hwy 297/Hedgeville Rd Wilks Ranch Montana LTD X X Roberts Creek 46.4535 -109.5030 at Hwy 297 (d/s of bridge) Pirrie, Joseph D X X Careless Creek, upper 46.4075 -109.3792 b/w Roberts Cr. and Swimming Woman Cr. above Wallum Rd State of Montana X X Careless Creek, upper 46.3778 -109.2906 u/s from Deadmans Basin, below Swimming Woman Creek Trustee of the Robin Lemmon Zeier X X X X Deadman's Basin Outlet Canal 46.3718 -109.2923 near mouth below spillway Trustee of the Robin Lemmon Zeier X X Careless Creek, lower 46.3736 -109.2854 d/s from Deadmans Basin; below Jessie Rd. Trustee of the Robin Lemmon Zeier X X X X Careless Creek, lower 46.3631 -109.2684 at Hwy 238 Bridge Quad Five Ranch; Wiley Micks, owner X X Careless Creek, lower 46.3554 -109.2430 at bridge past house and office on Quad Five Ranch Quad Five Ranch; Wiley Micks, owner X X Careless Creek, lower 46.3349 -109.2169 north of Sterling Rd. State Land; Leasee is X X Careless Creek, lower 46.3302 -109.1980 at Sterling Rd./Ninemile Rd. Colson, Chris & Etal X X X X Careless Creek, lower 46.3164 -109.1869 near mouth at Hwy 12 Parker, Clyde W and Sharon R X X Cottonwood Creek 46.3111 -110.4185 near headwaters Crazy M Ranch, LLP. X X X X X Cottonwood Creek 46.3376 -110.4050 at Cottonwood Cr Rd Teig Sr Ronald E & Teig Linda K. X X X Cottonwood Creek 46.3840 -110.3933 mid-segment, below Lost Horse Cr. 71 Ranch LP X X X Cottonwood Creek 46.4336 -110.4070 near mouth and Hwy 294 crossing 71 Ranch LP OR Cottonwood Cabins, LLC. X X X X X Deadmans Basin Reservoir 46.3280 -109.4288 at Bluebird Campground FWP X X Deadmans Basin Reservoir 46.3346 -109.4440 at Breezy Point, near Ditch inlet FWP or MSWC? X X

26 WQPBMASSAP-19 TN, Benthic E. Waterbody Name Latitude Longitude Site Description Owner(s) TP, NH DO Peri Metals 3+4 algae coli NO2+3 Delphia/Melstone South Canal 46.7249 -107.8269 above Musselshell confluence ROEN CLIFF A & LINDA X X Fish Creek 46.2024 -110.0026 near headwaters The Glennie Ranches, Inc. X Fish Creek 46.2321 -109.8955 at Hwy 191 Cooney Brothers Family Lmtd Partnership X X X Fish Creek 46.2525 -109.7688 below Hwy 191 Cooney Brothers Family Lmtd Partnership X Fish Creek 46.2118 -109.5899 below S Shamut Rd Adams, Harlan C X Fish Creek 46.2334 -109.4578 below Simmons Creek Taber, Frederick B X Fish Creek 46.2794 -109.2580 at Hwy 300 S of Ryegate Wipf and Morlee X X X Flatwillow Creek 46.9558 -108.0747 above Boxelder Creek Twin Creek Farm and Ranch, LLC X X Half Breed Creek 46.2703 -108.4706 near headwaters, above Rehder Creek Montana State Trust Lands X X X X Half Breed Creek 46.3175 -108.4884 below Rehder Creek Runestad, John Jr X X Half Breed Creek 46.3622 -108.4977 at Hwy 87 Funk, Jon X X Half Breed Creek 46.3872 -108.5418 at USGS site Half Breed Creek near Klein MT Bloomdahl, Huetta A X X Musselshell County; Klein Home and Orchard Half Breed Creek 46.4175 -108.5610 1 mile u/s from mouth subdivision? X X X X Martinsdale Reservoir 46.4501 -110.2772 nearest to fishing access facility FWP X

Martinsdale Reservoir 46.4519 -110.2717 near Dam FWP X

Mill Creek 46.6629 -110.5598 0.5 mile above Jamison Tr (FR47 or 2019?) crossing USFS X X X X - Mill Creek 46.6544 110.562075 0.5 mile below Jamison Tr (FR47 or 2019?) crossing USFS 46.654366 X X Mill Creek 46.6422 -110.5729 near mouth Bair Co X X X X Musselshell River, MT40A001_010 46.4088 -109.6981 below American Fork Clements Ranch Company LLC. X X X X below Antelope Creek and Harlow WWTP discharge, above American Musselshell River, MT40A001_010 46.4169 -109.7776 Leary, Gene G Fork X X X X Musselshell River, MT40A001_010 46.4289 -109.8410 at Harlowton, At USGS site 06120500 Hickman Linda X X X X X X Musselshell River, MT40A001_010 46.4301 -110.0722 near Two Dot WHELAN JOHN H (U/S owner) X X X Musselshell River, MT40A001_010 46.4301 -110.0722 near Two Dot WHELAN JOHN H (U/S owner) X X X Musselshell River, MT40A001_010 46.4636 -110.2264 Selkirk FAS FWP X X X X Musselshell River, MT40A001_010 46.4770 -110.2490 near Martinsdale; AT USGS site 06119600, below Martinsdale Colony BERG LAURETTA M X X X X X X Musselshell River, MT40A001_020 46.2891 -108.9380 above Big Coulee Creek at Lavina; At USGS Site 06125600 Bilden, Dan A X X X X Musselshell River, MT40A001_020 46.2939 -109.2581 Above Rygate; South of Hwy 12 on Rt 300, at Ryegate Bridge GOLDEN VALLEY COLONY INC (U/S owner) X X X X X X Musselshell River, MT40A001_020 46.3084 -109.1914 b/w Fish Cr. and Careless Cr., at USGS site 06123500 Garfield, Jess H X X X X Musselshell River, MT40A001_020 46.3190 -109.4610 above Mud Cr nr Shawmut; At USGS site 06123030 O'Connor, Trustee of Roy S 2005 Revocable Trust X X X X X X Musselshell River, MT40A001_020 46.3483 -108.6758 above Currant and Goulding Creeks Vescovi Polled Herefords X X X X Musselshell River, MT40A001_020 46.4277 -108.5746 between Horsethief and Halfbreed Rodeghiero Land LLC X X X X Musselshell River, MT40C001_010 46.4280 -108.5730 near Roundup; At USGS site 06126500 Holmes, Bert L X X X X Musselshell River, MT40C001_010 46.4391 -108.5370 above Roundup WWTP Musselshell County X X X X X X Musselshell River, MT40C001_010 46.4528 -108.4796 below Roundup WWTP and above Willow Cr. Montana State Trust Lands X X X X Musselshell River, MT40C001_010 46.5224 -108.1090 at Musselshell; At USGS Site 06127500, off of Hwy 12 Musselshell Ranch Co. X X X X

27 WQPBMASSAP-19 TN, Benthic E. Waterbody Name Latitude Longitude Site Description Owner(s) TP, NH DO Peri Metals 3+4 algae coli NO2+3 Musselshell River, MT40C001_010 46.6213 -107.8218 above Home Creek, below Hwy 12 crossing Territorial Holdings LLC. X X X X Musselshell River, MT40C001_010 46.7199 -107.8222 at Bridge Rd bridge ROEN CLIFF A & LINDA X X X X X Musselshell River, MT40C001_010 46.8494 -107.8925 above North Willow Cr. confluence BENSON JOHN X X X X Musselshell River, MT40C001_010 46.9225 -107.9199 above Flatwillow Creek, at USGS Site 06127600 near Mosby Woodford, Louanne X X X X Hwy right of way (east:Shaw Anna & Orval & Brett; Musselshell River, MT40C003_010 46.9947 -107.8890 at Mosby MT; At USGS site 06130500 west: Shaw, Bud T.) X X X X X Musselshell River, MT40C003_010 47.1674 -107.9473 above Calf Creek Browning, Theodore T III X X X Musselshell River, MT40C003_010 47.2103 -107.9353 below Calf Creek Browning, Ted T III & Travis J X X X Musselshell River, MT40C003_010 47.2818 -107.9611 below Dovetail along 79 Trail Heuker, Tim & Tom & Dan X X X Musselshell River, MT40C003_010 47.3097 -107.9556 near mouth 73 Ranch & Hunt Club, LLP. X X X X X near headwaters, below wetland complex where more likely to be North Fork Musselshell River, upper 46.6602 -110.5925 water BLM X X X near headwaters (power line intersection due west is at -110.601831 North Fork Musselshell River, upper 46.7102 -110.5967 46.709365) USFS X X X North Fork Musselshell River, upper 46.6401 -110.5736 near ranch road crossing below Mill Creek Bair Co; CareOfTaxpayer Glen Hough X X X North Fork Musselshell River, upper 46.6145 -110.5770 near ranch road crossing above Bair Reservoir and below Copper Cr Bair Co X X X X X North Fork Musselshell River, lower 46.5751 -110.5539 below Bair Reservoir Hayner, Kathy J (CareOfTaxPayer Ronald E Nelson?) X X X X X North Fork Musselshell River, lower 46.5728 -110.5366 Below Checkerboard and Brooks Creek confluence Hayner, Kathy J (CareOfTaxPayer Ronald E Nelson?) X X X North Fork Musselshell River, lower 46.5628 -110.5107 below Trail Creek Checkerboard Cattle Company, LLC. X X X North Fork Musselshell River, lower 46.5417 -110.4429 near Spring Creek confluence PMB Investments but managed by Richard L Indreland X X X North Fork Musselshell River, lower 46.4866 -110.3010 near mouth, off of Hwy 12 north of Martinsdale Teig, Michael O X X X X X North Fork Musselshell River, lower 46.4815 -110.2750 just above mouth Martinsdale Colony, Inc. X X X North Willow Creek 46.7212 -107.9994 Off of Ragged Point Rd State Land; Leasee is X X North Willow Creek 46.6007 -108.4127 300 yards d/s from Griffith Road Milton Ranch, LLC. X X X X North Willow Creek 46.6254 -108.3037 above Little Wall, at N Gage Rd Goffena, Deborah (mail to); 54 Livestock Inc. X X North Willow Creek 46.6663 -108.1550 below Little Wall; at Fourmile Rd ~16 miles N of Musselshell Musselshell Ranch Co. X X North Willow Creek 46.8433 -107.9000 At hwy 500 (Melstone Rd) BENSON JOHN X X X X Painted Robe Creek 46.0915 -109.2004 uppermost site near headwaters Rapelje Painted Rock Limited Partnership X X Painted Robe Creek 46.1307 -109.0862 mid-segment Jansen Livestock Co. X X Painted Robe Creek 46.1861 -108.9889 at lower crossing on Painted Road Road Montana State Trust Lands X X X X Painted Robe Creek 46.2090 -108.9227 at Buffalo Road and Hwy 3 Jensen, Craig; J C Jensen, Inc. X X Painted Robe Creek 46.2702 -108.8225 at Three Pines Road crossing near mouth Ballard, James D.; Ballard Ranch, Inc. X X X X South Fork McDonald Creek 47.0409 -108.8120 at Hwy 87/200 Negaard X X near headwaters at Bruno below confluence of Dry Fork and Warm South Fork Musselshell River 46.3733 -110.6250 Rostad & Rostad Springs X X X South Fork Musselshell River 46.4132 -110.5437 at Lennep Rd crossing Nicholes, Postell M & Kathryn G. H. X X X X X South Fork Musselshell River 46.4533 -110.3820 at USGS site 06118500, (71 Ranch LP) off MT 294 71 Ranch LP X X X South Fork Musselshell River 46.4531 -110.3557 off Martinsdale Rd. near mouth State Land; Leasee is X X X South Fork Musselshell River 46.4660 -110.3171 at Route 294 bridge near mouth BAIR CO C/O PAM HEDRICH X X X X X

28 WQPBMASSAP-19 TN, Benthic E. Waterbody Name Latitude Longitude Site Description Owner(s) TP, NH DO Peri Metals 3+4 algae coli NO2+3 South Fork Musselshell River 46.4792 -110.2754 near mouth, downstream from USGS gage Martinsdale Colony, Inc. X X X Trail Creek 46.6726 -110.5115 near headwaters, above mining influence USFS X X Trail Creek 46.6443 -110.5177 Below headwaters, at NFD2019 USFS X X X X Trail Creek 46.6046 -110.5181 mid-segment Checkerboard Cattle Company, LLC. X X Trail Creek 46.5645 -110.5123 near mouth Checkerboard Cattle Company, LLC. X X X X

29 WQPBMASSAP-19