DOCSLIB.ORG

Molecular Matchmaking Between the Popular Weight-Loss Herb Hoodia Gordonii and GPR119, a Potential Drug Target for Metabolic Disorder

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Molecular Matchmaking Between the Popular Weight-Loss Herb Hoodia Gordonii and GPR119, a Potential Drug Target for Metabolic Disorder Molecular matchmaking between the popular weight-loss herb Hoodia gordonii and GPR119, a potential drug target for metabolic disorder Shuyong Zhanga,b,1, Yuyong Mac,1, Jing Lib, Junjun Mac, Biao Yuc,2, and Xin Xiea,b,2 aShanghai Key Laboratory of Signaling and Disease Research, Laboratory of Receptor-Based Bio-Medicine, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China; bChinese Academy of Sciences Key Laboratory of Receptor Research, National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; and cState Key Laboratory of Bio-Organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China Edited by Robert J. Lefkowitz, Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC, and approved August 19, 2014 (received for review December 29, 2013) African cactiform Hoodia gordonii (Asclepiadaceae) has been used The plant is rich in pregnane glycosides containing 6-deoxy- for thousands of years by Xhomani Bushmen as an anorexant and 2,6-dideoxy-sugars. To date, P57AS3 (P57) is the only bi- during hunting trips and has been proposed as a new agent for ologically active constituent from H. gordonii that has been the management of body weight. However, its in vivo targets and reported to have anorexigenic activity (5). There is no evidence molecular mechanisms remain elusive. GPR119, a G protein-cou- of P57 binding to or altering activity of known receptors or pled receptor highly expressed in pancreatic β cells and intestinal proteins, but intracerebroventricular injections of P57 in rats has L cells, has been demonstrated to facilitate glucose-stimulated in- been reported to increase ATP content in the hypothalamus, sulin secretion (GSIS) and represents a novel and attractive target which might represent a signal of satiety and suppress appetitive for the therapy of metabolic disorders. Here, we disclose that responses (6). However, a recent study claimed that P57 was Gordonoside F (a steroid glycoside isolated from H. gordonii), not detectable in the brain upon oral administration in mice, but not the widely known P57, activates specifically GPR119. Suc- which makes the mechanisms of action of P57 and H. gordonii cessful synthesis of Gordonoside F facilitates further characteriza- PHARMACOLOGY more elusive (7). The development and commercialization of tion of this compound. Gordonoside F promotes GSIS both in vitro and in vivo and reduces food intake in mice. These effects are medi- H. gordonii and P57 have been problematic due to controversy ated by GPR119 because GPR119 knockout prevents the therapeutic about intellectual property rights and limited natural resources, effects of Gordonoside F. Interestingly, the appetite-suppressing ef- in addition to the lack of a clear mechanism, resulting in the fect of Hoodia extract was also partially blocked by GPR119 knockout. withdrawal of several major pharmaceutical companies from Our results demonstrate for the first time, to our knowledge, Hoodia projects (3). Since the isolation of P57, more new gly- cosides, including Gordonoside A-L (8), have been disclosed in that GPR119 is a direct target and one of the major mechanisms CHEMISTRY underlying the therapeutic effect of the popular “weight loss” the literature although their bioactivities remain unclear. herb H. gordonii. Given the long history of safe application of Meanwhile, many new potential drug targets related to met- this herb in weight control, it is foreseeable that the novel scaf- abolic disorders have been identified. Compounds targeting fold of Gordonoside F provides a promising opportunity to de- a number of G protein-coupled receptors (GPCRs), including velop new drugs in treating metabolic diseases. Significance besity is one of the most common health concerns, affecting Omore than 300 million people worldwide (1). Obesity is Obesity is one of the most common health concerns today. associated with and can lead to a number of diseases, including Herbal-based food supplements are widely used to treat obe- type 2 diabetes (T2D), cardiovascular disease, osteoarthritis, sity. Among them, the African cactiform Hoodia gordonii sup- dyslipidemia, and sleep apnea, many of which can be prevented plements are extremely popular. The development of Hoodia with a reduction in body weight (1). Current strategies for the and P57, the purported active ingredient, has been problematic management of body weight include calorie restriction, regular due to controversy about intellectual property rights and lim- exercise, and behavior modification. Although effective in the ited natural resources. To date, the true active components and molecular targets of Hoodia remain unclear. Here, we dem- short term, diet and exercise alone are difficult to maintain over onstrate that Gordonoside F, a compound from Hoodia, acti- the long term for the majority of patients. Thus, medications and vates GPR119, a receptor critically involved in metabolic alternative treatments have been sought. The commercial market homeostasis, and leads to increased insulin secretion and re- for antiobesity preparations is enormous, but unfortunately, there duced food intake. The successful synthesis of Gordonoside F are only two FDA-approved drugs available, phentermine and described here will provide an opportunity for developing new orlistat, both with limited efficacy but significant side effects (2). drugs in treating metabolic diseases. Herbal-based food supplements are among the most widely used alternative treatments for obesity. However, their effec- Author contributions: B.Y. and X.X. designed research; S.Z., Y.M., J.L., and J.M. performed tiveness, safety, and mechanism of actions largely remain un- research; S.Z., Y.M., J.L., J.M., B.Y., and X.X. analyzed data; and S.Z., Y.M., B.Y., and X.X. wrote the paper. known. Among them, the Hoodia gordonii supplements are extremely The authors declare no conflict of interest. popular. The African cactiform H. gordonii (Asclepiadaceae) is This article is a PNAS Direct Submission. a succulent plant growing in the Kalahari Deseret in South Freely available online through the PNAS open access option. Africa, Namibia, and Botswana (3). The plant has been used for 1S.Z. and Y.M. contributed equally to this work. thousands of years by Xhomani Bushmen as a hunger and thirst 2To whom correspondence may be addressed. Email: [email protected] or [email protected]. suppressant during hunting trips, and the natural antiobesity ac.cn. agent from the plant has attracted great attention and led to This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10. many commercial preparations (4). 1073/pnas.1324130111/-/DCSupplemental. www.pnas.org/cgi/doi/10.1073/pnas.1324130111 PNAS Early Edition | 1of6 Downloaded by guest on September 25, 2021 Table 1. Biological activities of the naturally occurring Hoodigosides/Gordonosides (1–6) and synthetic Gordonoside F on the human GPR119 receptor O O O R O O OH MeO O EC50, µM dnuopmoC R cAMP assay Calcium assay P57 (1) NR NR HO O MeO O O OH MeO Hoodigoside K (2) OH NR NR HO O O O HO HO OH HO OH Gordonoside F (3) 0.53 ± 0.12 9.3 ± 0.3 HO O MeO O O O O MeO MeO Hoodigoside E/Gordonoside H (4) OH NR NR HO O O O O HO MeO O OH OH MeO Hoodigoside G (5) OH NR NR HO O HO O O O O O OH O MeO MeO OH OMe Hoodigoside I (6) OH NR NR HO O HO O O O MeO O O OH O OMe OMe Gordonoside F (3, synthetic) 0.44 ± 0.02 3.2 ± 1.4 HO O MeO O O O O MeO MeO NR, no response at concentrations up to 100 μM. GLP-1R, GPR40, GPR120, CB1, GCGR, and β2-adenoceptor activates GPR119 potently and selectively, which consequently (9), have been proposed to treat T2D and obesity. Recently, promotes GSIS both in vitro and in vivo and reduces food intake GPR119, a GPCR highly expressed in pancreatic β cells and in animals. In addition, chemical synthesis of Gordonoside F is intestinal L cells (10, 11), has been demonstrated to facilitate reported. The present results not only demonstrate unambiguously glucose-stimulated insulin secretion (GSIS) (12). The endoge- that the activation of GPR119 receptor is an important mechanism nous ligands LPC and OEA promote GSIS, directly by activating underlying H. gordonii’s therapeutic effect but also suggest that GPR119 on the β cells and indirectly by activating GPR119 on Gordonoside F or its congeners could be developed into new drugs the L cells, and induce GLP-1 secretion (13). Oral administra- in treating metabolic disorders. tion of AR231453, a synthetic agonist of GPR119, significantly improves circulating insulin, GLP-1, and GIP levels, and lowers Materials and Methods the blood glucose concentration in glucose tolerance tests in Chemicals and Reagents. Gordonoside F and its natural congeners were pur- mice (11, 14). Another GPR119 agonist, PSN632408, has been chased from AnalytiCon Discovery and/or synthesized chemically (see SI Appendix, Materials and Methods for details). Crude extract of H. gordonii (Ref: EA149464) shown to suppress food intake and reduce body weight gain was provided by Naturex. Mammalian expression vectors encoding various in rats, in addition to its blood glucose-lowering effect (15). GPCRs and Gα16 were purchased from the UMR cDNA Resource Center. Therefore, GPR119 represents an attractive target for the ther- apy of diabetes and obesity. Reporter Assay. Cells expressing GPR119 (or other GPCRs) and CRE-luc were Here, we disclose that Gordonoside F (a steroid glycoside plated at a density of 10,000 cells per well in a 96-well plate. After 24 h culture, isolated from H. gordonii), but not the widely known P57, compounds at various concentrations were added. DMSO (1%) was used as 2of6 | www.pnas.org/cgi/doi/10.1073/pnas.1324130111 Zhang et al.
Load more

Recommended publications
  • Hoodia Gordonii (Masson) Sweet Ex Decne., 1844
    Hoodia gordonii (Masson) Sweet ex Decne., 1844 Identifiants : 16192/hoogor Association du Potager de mes/nos Rêves (https://lepotager-demesreves.fr) Fiche réalisée par Patrick Le Ménahèze Dernière modification le 02/10/2021 Classification phylogénétique : Clade : Angiospermes ; Clade : Dicotylédones vraies ; Clade : Astéridées ; Clade : Lamiidées ; Ordre : Gentianales ; Famille : Apocynaceae ; Classification/taxinomie traditionnelle : Règne : Plantae ; Sous-règne : Tracheobionta ; Division : Magnoliophyta ; Classe : Magnoliopsida ; Ordre : Gentianales ; Famille : Apocynaceae ; Genre : Hoodia ; Synonymes : Hoodia husabensis Nel, Hoodia langii Oberm. & Letty, Hoodia longispina Plowes, Hoodia pillansii N. E. Br, Hoodia rosea Oberm. & Letty, Hoodia whitsloaneana Dinter ex A. C. White & B. Sloane, Hoodia barklyi Dyer, Hoodia burkei N. E. Br, Hoodia bainii Dyer, Hoodia albispina N. E. Br ; Nom(s) anglais, local(aux) et/ou international(aux) : queen of the Namib, African hats , Bitterghaap, Ghoba, Wilde ghaap ; Rapport de consommation et comestibilité/consommabilité inférée (partie(s) utilisable(s) et usage(s) alimentaire(s) correspondant(s)) : Feuille (tiges0(+x) [nourriture/aliment{{{0(+x) et/ou{{{(dp*) masticatoire~~0(+x)]) comestible0(+x). Détails : Les tiges sont mâchées pour{{{0(+x) rassasier et entrainer la satiété{{{(dp*), par exemple dans le cas de régimes{{{(dp*) ; elles sont consommées fraîches comme un aliment ; elles ont un goût amer{{{0(+x). Les tiges sont mâchées pour réduire le désir de nourriture. Ils sont consommés frais comme aliment. Ils ont un goût amer néant, inconnus ou indéterminés.néant, inconnus ou indéterminés. Illustration(s) (photographie(s) et/ou dessin(s)): Curtis´s Botanical Magazine (vol. 102 [ser. 3, vol. 32]: t. 6228, 1876) [W.H. Fitch], via plantillustrations.org Page 1/2 Autres infos : dont infos de "FOOD PLANTS INTERNATIONAL" : Distribution : Une plante tropicale.
    [Show full text]
  • Biodiversity and Perspectives of Traditional Knowledge in South Africa
    WIPO SEMINAR ON INTELLECTUAL PROPERTY AND DEVELOPMENT 1 GENEVA: 2 -3 MAY 2005 BIODIVERSITY AND PERSPECTIVES OF TRADITIONAL KNOWLEDGE IN SOUTH AFRICA ABSTRACT The San peoples, known as marginalised“first peoples” indigenous to Africa, have over the past five years rapidly discovered the meaning of the words biodiversity and tradi tional knowledge, whilst being drawn in to the hitherto arcane and irrelevant world of intellectual property and international trade. This paper briefly traverses the case of the patenting of an extract of the Hoodia Gordonii, one of the many plant produc ts used for medicinal purposes (as an appetite suppressant) by the San, and the subsequent developments in the San world as well as on a national policy level. Some issues arising during the case such as the requirement of prior informed consent, the furt her articulation of the San’s collective legal rights, benefit sharing and intellectual property issues relative to traditional knowledge of indigenous peoples, are discussed. 1 THE SAN PEOPLES The San peoples of Southern Africa are the acknowledged “F irst Peoples” of Africa, widely touted as the holders of the oldest genes known to man. Their numbers are now reduced to approximately 100 000 in Botswana, Namibia and Angola and South Africa 1, where they generally inhabit hostile environments, live close to nature, and survive in poverty on the fringes of the emerging African societies. It is relevant to the story of the San’s involvement in the current debate on biodiversity, traditional knowledge and intellectual property that they formed their own net working organisation in 1996, in order to better connect with the growing indigenous peoples movement and the UN Decade on Indigenous Peoples.
    [Show full text]
  • An Updated Snapshot of Recent Advances in Transcriptomics and Genomics of Phytomedicinals Biswapriya B
    PostDoc Journal Journal of Postdoctoral Research Vol. 2, No. 2, February 2014 www.postdoctoraljournal.com An Updated Snapshot of Recent Advances in Transcriptomics and Genomics of Phytomedicinals Biswapriya B. Misra Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32610, USA Email: [email protected] Abstract Medicinal plants have been of great importance to human health care since the advent of medicine. A huge array of molecules has been obtained from these phytopharmaceutical-yielding species that have influenced human lives since the beginning of plant-based life-saving medicines. Some of these molecules have taken the form of taxol, aspirin, and artemisinin. With the flourishing era of high- throughput next generation sequencing technologies, a hot pursuit for sequencing the genomes and transcriptomes of these life-saving plants is underway. Although few genomes have been sequenced or are currently being addressed, the number of transcriptomes sequenced has sky-rocketed in the last couple of years and continues to surge forward with immense pace, covering all important genera of medicinal plants. I have attempted to provide the current status, progress, opportunities, and challenges of these sequencing endeavors in this comprehensive and updated review. It is my hope that this information will provide both specialists and non-specialists with the current trends and future directions of this interesting category of plants. Keywords: medicinal plant, metabolic pathway, genome, next generation sequencing, transcriptome Introduction Metabolites are small chemical entities present medicine are generally known as medicinal in living organisms with a molecular weight of plants. They belong to typical taxonomic families, less than 1000 Da.
    [Show full text]
  • Sotwp 2016.Pdf
    STATE OF THE WORLD’S PLANTS OF THE WORLD’S STATE 2016 The staff and trustees of the Royal Botanic Gardens, Kew and the Kew Foundation would like to thank the Sfumato Foundation for generously funding the State of the World’s Plants project. State of the World’s Plants 2016 Citation This report should be cited as: RBG Kew (2016). The State of the World’s Plants Report – 2016. Royal Botanic Gardens, Kew ISBN: 978-1-84246-628-5 © The Board of Trustees of the Royal Botanic Gardens, Kew (2016) (unless otherwise stated) Printed on 100% recycled paper The State of the World’s Plants 1 Contents Introduction to the State of the World’s Plants Describing the world’s plants 4 Naming and counting the world’s plants 10 New plant species discovered in 2015 14 Plant evolutionary relationships and plant genomes 18 Useful plants 24 Important plant areas 28 Country focus: status of knowledge of Brazilian plants Global threats to plants 34 Climate change 40 Global land-cover change 46 Invasive species 52 Plant diseases – state of research 58 Extinction risk and threats to plants Policies and international trade 64 CITES and the prevention of illegal trade 70 The Nagoya Protocol on Access to Genetic Resources and Benefit Sharing 76 References 80 Contributors and acknowledgments 2 Introduction to the State of the World’s Plants Introduction to the State of the World’s Plants This is the first document to collate current knowledge on as well as policies and international agreements that are the state of the world’s plants.
    [Show full text]
  • Hoodia Gordonii SCORE: 0.0 RATING: Low Risk
    TAXON: Hoodia gordonii SCORE: 0.0 RATING: Low Risk Taxon: Hoodia gordonii Family: Apocynaceae Common Name(s): ghaap Synonym(s): Hoodia barklyi Dyer hoodia Hoodia burkei N. E. Br. Hoodia longispina Plowes Stapelia gordonii Masson Assessor: Chuck Chimera Status: Assessor Approved End Date: 15 Apr 2015 WRA Score: 0.0 Designation: L Rating: Low Risk Keywords: Succulent, Spiny, Medicinal, Fly-Pollinated, Wind-Dispersed Qsn # Question Answer Option Answer 101 Is the species highly domesticated? y=-3, n=0 n 102 Has the species become naturalized where grown? 103 Does the species have weedy races? Species suited to tropical or subtropical climate(s) - If 201 island is primarily wet habitat, then substitute "wet (0-low; 1-intermediate; 2-high) (See Appendix 2) High tropical" for "tropical or subtropical" 202 Quality of climate match data (0-low; 1-intermediate; 2-high) (See Appendix 2) High 203 Broad climate suitability (environmental versatility) y=1, n=0 n Native or naturalized in regions with tropical or 204 y=1, n=0 y subtropical climates Does the species have a history of repeated introductions 205 y=-2, ?=-1, n=0 ? outside its natural range? 301 Naturalized beyond native range y = 1*multiplier (see Appendix 2), n= question 205 n 302 Garden/amenity/disturbance weed n=0, y = 1*multiplier (see Appendix 2) n 303 Agricultural/forestry/horticultural weed n=0, y = 2*multiplier (see Appendix 2) n 304 Environmental weed n=0, y = 2*multiplier (see Appendix 2) n 305 Congeneric weed n=0, y = 1*multiplier (see Appendix 2) n 401 Produces spines, thorns
    [Show full text]
  • WO 2016/034942 Al 10 March 2016 (10.03.2016) P O P C T
    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2016/034942 Al 10 March 2016 (10.03.2016) P O P C T (51) International Patent Classification: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, C07H 1/08 (2006.01) A23L 1/22 (2006.01) DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, C07H 15/256 (2006.01) HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, (21) International Application Number: MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PCT/IB20 15/00 1879 PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, (22) International Filing Date: SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, 27 August 2015 (27.08.2015) TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (25) Filing Language: English (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (26) Publication Language: English GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, (30) Priority Data: TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, 62/044,626 2 September 2014 (02.09.2014) US TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, 62/059,562 3 October 2014 (03. 10.2014) US DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, 62/061,363 8 October 2014 (08.
    [Show full text]
  • (12) Patent Application Publication (10) Pub. No.: US 2007/0104805 A1 Udel (43) Pub
    US 20070 104805A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2007/0104805 A1 Udel (43) Pub. Date: May 10, 2007 (54) COMPOSITIONS OF HOODIA GORDONII Related U.S. Application Data AND PINOLENIC ACID DERVATIVES (60) Provisional application No. 60/732,440, filed on Nov. (76) Inventor: Ronald G. Udell, Beverly Hills, CA 1, 2005. (US) Publication Classification Correspondence Address: DORSEY & WHITNEY LLP (51) Int. Cl. INTELLECTUAL PROPERTY DEPARTMENT A6IR 36/3 (2006.01) SUTE 15OO A6II 3 L/704 (2006.01) SO SOUTH SIXTH STREET A6IR 36/85 (2006.01) MINNEAPOLIS, MN 55402-1498 (US) (52) U.S. Cl. ............................. 424/725; 424/770; 514/26 (57) ABSTRACT (21) Appl. No.: 11/554,982 The present invention is directed to compositions containing a pine nut oil and an extract from the genus Hoodia or (22) Filed: Oct. 31, 2006 Trichocaulon and methods of treatment for weight loss. US 2007/0104805 A1 May 10, 2007 COMPOSITIONS OF HOODA GORDON AND maintain a healthy weight. Weight gain is often a combina PNOLENIC ACID DERVATIVES tion of reduced metabolism of glucose, the individuals inability to block carbohydrate absorption from the diet CROSS-REFERENCE TO RELATED and/or the inability to reduce body fat. A solution to one or APPLICATION(S) more of these problems is sought by many people, young and old alike 0001) This application claims the benefit of U.S. Provi sional Application No. 60/732,440, filed Nov. 1, 2005, the 0009. There is a need in the art for a nutritional supple entire contents of which is incorporated herein by reference.
    [Show full text]
  • The Making and Unmaking of Patent Ownership: Technicalities, Materialities, and Subjectivities
    May 2016 Page 127 Laura A. Foster Indiana University, Bloomington The Making and Unmaking of Patent Ownership: Technicalities, Materialities, and Subjectivities Feminist sociolegal studies have recently taken up the technicalities of doctrines, docu- ments, and regulations to better understand the law. In an affiliated move, feminist science studies turned to the materialities of theories, practices, and nonhuman organisms to make critical sense of science. These methodological turns focus not on gender, per se, but on precise mechanisms of law and science that structure, reinforce, and reconfigure power and inequality. Drawing on these methodological approaches, this article attends to the technicalities and materialities of patent ownership and benefit sharing in South Africa in regards to San peoples’ struggles over the patenting of the Hoodia gordonii plant. An examination of patent documents, benefit-sharing agreements, legislative appendixes, and the biology of plants generates an understanding of how patent ownership, rather than being natural or value-neutral, is a historical and sociocultural process shaping, refash- ioning, and being inscribed across multiple scales of nation-state jurisdictions, divergent ways of knowing, and biochemical orderings of plants. [indigenous knowledge, Southern Africa, patent law, benefit sharing, feminist studies] Scientists with the South African Center for Scientific and Industrial Research (CSIR) obtained a provisional patent in 1997 related to the Hoodia gordonii plant, which they found to be
    [Show full text]
  • Journal of Herbs, Spices & Medicinal Plants Identification And
    This article was downloaded by: [USDA National Agricultural Library] On: 29 January 2010 Access details: Access Details: [subscription number 911188449] Publisher Taylor & Francis Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House, 37- 41 Mortimer Street, London W1T 3JH, UK Journal of Herbs, Spices & Medicinal Plants Publication details, including instructions for authors and subscription information: http://www.informaworld.com/smpp/title~content=t792306868 Identification and Differentiation between Hoodia gordonii (Masson) Sweet ex Decne., Opuntia ficus indica (L.) P. Miller, and Related Hoodia Species Using Microscopy and PCR Vaishali Joshi a; Natascha Techen a; Brian E. Scheffler b; Ikhlas A. Khan a a National Center for Natural Products, Research and Research Institute of Pharmaceutical Sciences Department of Pharmacognosy, School of Pharmacy, University of Mississippi, Mississippi, USA b USDA-ARS MSA Genomics Laboratory, Stoneville, Mississippi, USA Online publication date: 14 December 2009 To cite this Article Joshi, Vaishali, Techen, Natascha, Scheffler, Brian E. and Khan, Ikhlas A.(2009) 'Identification and Differentiation between Hoodia gordonii (Masson) Sweet ex Decne., Opuntia ficus indica (L.) P. Miller, and Related Hoodia Species Using Microscopy and PCR', Journal of Herbs, Spices & Medicinal Plants, 15: 3, 253 — 264 To link to this Article: DOI: 10.1080/10496470903378953 URL: http://dx.doi.org/10.1080/10496470903378953 PLEASE SCROLL DOWN FOR ARTICLE Full terms and conditions of use: http://www.informaworld.com/terms-and-conditions-of-access.pdf This article may be used for research, teaching and private study purposes. Any substantial or systematic reproduction, re-distribution, re-selling, loan or sub-licensing, systematic supply or distribution in any form to anyone is expressly forbidden.
    [Show full text]
  • WO 2014/151206 Al 25 September 2014 (25.09.2014) P O P C T
    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2014/151206 Al 25 September 2014 (25.09.2014) P O P C T (51) International Patent Classification: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, C07K 7/08 (2006.01) A61P 1/00 (2006.01) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, A61K 38/10 (2006.01) A61P 29/00 (2006.01) DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, (21) International Application Number: KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, PCT/US20 14/025207 MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (22) International Filing Date: OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, 13 March 2014 (13.03.2014) SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, (25) Filing Language: English ZW. (26) Publication Language: English (84) Designated States (unless otherwise indicated, for every (30) Priority Data: kind of regional protection available): ARIPO (BW, GH, 61/790,266 15 March 2013 (15.03.2013) US GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, 61/826,749 23 May 2013 (23.05.2013) US UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, (71) Applicant: SYNERGY PHARMACEUTICALS INC.
    [Show full text]
  • A Systematic Review of the European Rapid Alert System for Food and Feed: Tendencies in Illegal Food Supplements for Weight Loss
    SYSTEMATIC REVIEW published: 26 January 2021 doi: 10.3389/fphar.2020.611361 A Systematic Review of the European Rapid Alert System for Food and Feed: Tendencies in Illegal Food Supplements for Weight Loss Dorottya Koncz 1, Barbara Tóth 1,2, Orsolya Roza 1,2 and Dezso} Csupor 1,2* 1Department of Pharmacognosy, University of Szeged, Szeged, Hungary, 2Medical School, Institute for Translational Medicine, University of Pécs, Pécs, Hungary Background: Slimming products represent a dynamically growing group of food supplements worldwide. The efficacy of safely usable natural ingredients is usually below consumers’ expectations. Certain manufacturers add unauthorized or prohibited ingredients to weight loss supplements in order to increase their efficacy. Hence, many of these products are adulterated and may pose a risk to the consumers’ health. Aims: The aim of our work was to give an overview on natural ingredients used in slimming products, to summarize the frequently used synthetic adulterants and also to assess the Edited by: trends of adulterated and illegal food supplements in the European Union based on the Michał Tomczyk, Medical University of Bialystok, Poland warnings of the Rapid Alert System for Food and Feed (RASFF) in the time period of – Reviewed by: 1988 2019. Eric Deconinck, – Sciensano, Belgium Methods: Reports between 1988 2019 were extracted from the RASFF portal on Jakub Dorozy˙ nski, ´ January 1, 2020. Each entry was individually reviewed. Department of Family Medicine, Medical University of Lodz, Poland Results: 2,559 records of food supplements with quality problems were identified in the *Correspondence: RASFF, several of which [319 (12,5%)] were marketed to facilitate weight loss.
    [Show full text]
  • (African Plant), Caralluma Fimbriata and Achyranthes Aspera (Indian Plants): an Appetite Suppressant
    id20199281 pdfMachine by Broadgun Software - a great PDF writer! - a great PDF creator! - http://www.pdfmachine.com http://www.broadgun.com BBiiooTTISSNee : 09cc74 - hh7435 nnoollVolooume g7g Issuyye 8 An Indian Journal Review BTAIJ, 7(8), 2013 [285-290] Hoodia gordonii (African plant), Caralluma fimbriata and Achyranthes aspera (Indian plants): An appetite suppressant Boni Topiwala, R.Krishnamurthy* C.G Bhakta Institute of Biotechnology, Maliba Campus, Uka Tarsadia University, Mahuva Road, Bardoli- 394601, Surat, Gujarat, (INDIA) E-mail : [email protected]; [email protected] ABSTRACT KEYWORDS Achyranthes aspera, Caralluma fimbriata an Indian origin plant and Achyranthes aspera; Hoodia gordonii, an African origin plant, an incredible natural flora for Caralluma fimbriata; reducing the voracious hunger and an intimate way to reduce the stout- Hoodia gordonii; ness. The phytochemical constitute is the eventual innate boon in the Appetite suppressant; both the plants and the pupil claim to overcome their appetite. A miracu- Phytochemical constituents. lous discovery that had lead to reduce hunger in the poor people from starving crisis. 2013 Trade Science Inc. - INDIA INTRODUCTION life. “Now good digestion wait on appetite, And health on ACHYRANTHES ASPERA (APAMARGA) ” both! -William Shakespeare (Macbeth) Morphology Wisely said proverb, fitness and hunger are the ’s the most vital need It is an erect herb. Widely distributed in Northern foremost need of living beings. It part of India and is widely used as a folk medicine, to withstand existence. Water is the primary need to Southern India and in the some areas for Gujarat. It lessen hunger, but one can only get energy through food. mainly grows in the season of Monsoon.
    [Show full text]