A Region bacterivore omnivore plant parasite eukaryvore

● ● ● 20 40 ● ● ● 60 ● ● ● ● ● 40 15 ● 30

● ● ● ● 40 ● ● ● 10 ● ● 20 ● ●

● 20

Relative abundance (%) Relative abundance 5 20 0 b a b 10 c b a a a a a a a Alb Hainich Schorfheide Alb Hainich Schorfheide Alb Hainich Schorfheide Alb Hainich Schorfheide

B type bacterivore omnivore plant parasite eukaryvore

● ● ● ● ● 20 40 ● ● ● ● ● ● 60 ● ● ● ● 40 ● 15 ● ● ● ● 30 ● ● ● ● ● ● ● ● ● ● ● ● ● ● 40 ● ● ● ● ● ● ● 20 10 ● ● ● ● ● ● ● ● ● 20 ● ●

Relative abundance (%) Relative abundance ●

● 20 5 0 10 b ac ab a bc bc ab ab b b ab ab a a ab ab a a a a a a a a a a a a a a a a Luvisol Luvisol Luvisol Luvisol Vertisol Vertisol Vertisol Gleysol Gleysol Gleysol Histosol Histosol Histosol Leptosol Leptosol Leptosol Leptosol Cambisol Cambisol Cambisol Stagnosol Stagnosol Stagnosol Albeluvisol Albeluvisol Albeluvisol Albeluvisol

C land usage bacterivore omnivore plant parasite eukaryvore

● ● ● ● ● 40 20 ● ● 60 ● ● ● ● ● 40 ● ● 15 ● 30

● ● 40 ● ● ● ● ● 20 ● ● 10

● ● 20 ● Relative abundance (%) Relative abundance 20 5 10 0 a a a a a a b a a a a a pasture mowed meadow pasture mowed meadow pasture mowed meadow pasture mowed meadow meadow meadow meadow meadow

D LUI bacterivore omnivore plant parasite eukaryvore

● ● ● ● ● 40 ● ● ● ● ● 20 60 ● ● ● ● 40 ● ● ● ● 30 ● 15 ● ● ● ● 40 ● ● ● ● 20 ● ● ● 20 ● 10 Relative abundance (%) Relative abundance

● 20 ● ● 10 0 ● b a b b a a a c b 5 b a a ● low moderate high low moderate high low moderate high low moderate high

E C/N ratio bacterivore omnivore plant parasite eukaryvore

● ● ● 20 ● 40 ● ● ● ● 60 ● ● 40 15 ● ● ● ● ● ● 30 ●

● ● ● 40 ● 10 ● ● ● ● 20 ● ● ● 20 Relative abundance (%) Relative abundance 5 20 a a a 10 b a a 0 a b b b a a - >10 - 15 ~10 7 - <10 >10 - 15 ~10 7 - <10 >10 - 15 ~10 7 - <10 >10 - 15 ~10 7 - <10

Figure S4. Boxplots of the variation of the relative abundances of the four main nutrition modes of Cercozoa and Endomyxa in grassland, colored according to region (approx. %). A. by region; B. by , only Cambisoil occurs in the three regions; C. by grassland management; D. by land use intensity (LUI) index, transformed into a categorical variable according to quantiles. E. by C/N ratio, transformed as in D. The y-scale varies between graphs. Red letters: a change from “a” to “b”, or “c” indicates a significant difference (multiple comparison of means, Tukey's test); two or three letters (e.g. “ab” or "abc") indicate non-significant differences between plots sharing those letters. Red lines indicate the mean. Right side, tables with the regional repartion of each factor; 100 sites in Alb, 96 in Hainich and 97 in Schorfheide.