Anti-CIDE a Antibody [V62P1E3*B10] (ARG10830)

Product datasheet [email protected]

ARG10830 Package: 100 μg anti-CIDE A antibody [V62P1E3*B10] Store at: -20°C

Summary

Product Description Mouse Monoclonal antibody recognizes CIDE A

Tested Reactivity Hu

Tested Application IHC-P, WB

Host Mouse

Clonality Monoclonal

Clone V62P1E3*B10

Isotype IgG1, kappa

Target Name CIDE A

Antigen Species Human

Immunogen Ovalbumin-conjugated synthetic peptide. (QAKGRFTCG)

Conjugation Un-conjugated

Alternate Names CIDE-A; Cell death-inducing DFFA-like effector A; Cell death activator CIDE-A

Application Instructions

Application table Application Dilution

IHC-P Assay-dependent

WB Assay-dependent

Application Note Antigen Retrieval: Boil tissue section for 10 - 20 min at 800 - 950W microwave with 10 mM Citrate buffer or Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Calculated Mw 25 kDa

Properties

Form Liquid

Purification Affinity purification with immunogen.

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation www.arigobio.com 1/3 Gene Symbol CIDEA

Gene Full Name cell death-inducing DFFA-like effector a

Background This gene encodes the homolog of the mouse protein Cidea that has been shown to activate apoptosis. This activation of apoptosis is inhibited by the DNA fragmentation factor DFF45 but not by caspase inhibitors. Mice that lack functional Cidea have higher metabolic rates, higher lipolysis in brown adipose tissue and higher core body temperatures when subjected to cold. These mice are also resistant to diet- induced obesity and diabetes. This suggests that in mice this gene product plays a role in thermogenesis and lipolysis. Alternatively spliced transcripts have been identified. [provided by RefSeq, Aug 2010]

Function Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter (By similarity). Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear. [UniProt]

Images

ARG10830 anti-CIDE A antibody [V62P1E3*B10] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded primary colorectal cancer tissue sections stained with ARG10830 anti-CIDE A antibody [V62P1E3*B10]. Antigen Retrieval: Boil tissue section in microwave 10 min at 950W in 0.01M Sodium citrate buffer (pH 6.0).

ARG10830 anti-CIDE A antibody [V62P1E3*B10] WB image

Western blot: Lysates prepared from cells overexpressing CIDE A (+) and cells transfected with an empty vector (-) stained with ARG10830 anti-CIDE A antibody [V62P1E3*B10].

www.arigobio.com 2/3 ARG10830 anti-CIDE A antibody [V62P1E3*B10] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer sections stained with ARG10830 anti-CIDE A antibody [V62P1E3*B10].