Lysosomes & Endocytosis

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Lysosomes & Endocytosis Lysosomes & Endocytosis Gordon Research Conference Molecular Mechanisms and Functions of Endosomal and Lysosomal Pathways June 12-17, 2016 Proctor Academy Andover, NH Chair: Roberto Weigert Vice Chair: Lois S. Weisman Contributors Meeting Program Sunday 2:00 pm - 9:00 pm Arrival and Check-in 6:00 pm Dinner 7:30 pm - 7:40 pm Welcome / Introductory Comments by GRC Site Staff 7:40 pm - 9:30 pm Endocytic Machinery at the Plasma Membrane Discussion Leaders: Sandra Schmid (University of Texas Southwestern Medical Center, USA) and Mark Von Zastrow (University of California, San Francisco, USA) 7:40 pm - 8:00 pm Volker Haucke (Leibniz-Institut für Molekulare Pharmakologie, Germany) "Phosphoinositide Conversion Within the Endolysosomal System" 8:00 pm - 8:10 pm Discussion 8:10 pm - 8:30 pm Ludger Johannes (Institut Curie, France) "Using Glycosphingolipids to Build Endocytic Pits in Clathrin-Independent Endocytosis" 8:30 pm - 8:40 pm Discussion 8:40 pm - 9:00 pm Margaret Robinson (University of Cambridge, United Kingdom) "Coated Vesicle Adaptors" 9:00 pm - 9:10 pm Discussion 9:10 pm - 9:25 pm Sara Sigismund (IFOM, The FIRC Institute for Molecular Oncology Foundation, Italy) "ER-PM Contact Sites Control EGFR Endocytosis" 9:25 pm - 9:30 pm Discussion Monday 7:30 am - 8:30 am Breakfast 9:00 am - 12:30 pm Endosomal Sorting and Recycling Discussion Leaders: Elizabeth Conibear (University of British Columbia, Canada) and Scott Emr (Cornell University, USA) 9:00 am - 9:20 am Peter Cullen (University of Bristol, United Kingdom) "Molecular Mechanisms of Endosomal Cargo Sorting and Export" 9:20 am - 9:30 am Discussion 9:30 am - 9:50 am Julie Donaldson (National Heart, Lung, and Blood Institute, NIH, USA) "Sorting of Endosomal Cargo: Role of Cytoskeleton and Motors" 9:50 am - 10:00 am Discussion 10:00 am - 10:15 am Manojkumar Puthenveedu (Carnegie Mellon University, USA) "GPCR Localization and Signaling in Endosomal Recycling Microdomains" 10:15 am - 10:20 am Discussion 10:20 am - 10:45 am Coffee Break 10:45 am - 11:05 am Erik Jorgensen (Howard Hughes Medical Institute / University of Utah, USA) "Ultrafast Endocytosis of Synaptic Vesicles" 11:05 am - 11:15 am Discussion 11:15 am - 11:35 am Julie Brill (The Hospital for Sick Children, Canada) "Regulation of Endosomal Sorting and Recycling by PI4P" 11:35 am - 11:45 am Discussion 11:45 am - 12:05 pm Aitor Hierro (CIC bioGUNE, Spain) "Cargo-Specific Sorting from Endosomes" 12:05 pm - 12:15 pm Discussion 12:15 pm - 12:25 pm Zuzana Kadlecova (University of Texas Southwestern Medical Center, USA) "Hierarchical Allosteric Regulation of AP2 Activity Controls Clathrin-Mediated Endocytosis" 12:25 pm - 12:30 pm Discussion 12:30 pm Lunch 1:30 pm - 4:00 pm Free Time 4:00 pm - 6:00 pm Poster Session 6:00 pm Dinner 7:30 pm - 9:30 pm Exploiting the Endocytic Machinery During Host-Pathogen Interactions Discussion Leaders: Sergio Grinstein (Hospital for Sick Children, Canada) and Fabienne Paumet (Thomas Jefferson University, USA) 7:30 pm - 7:50 pm Nihal Altan-Bonnet (National Institutes of Health, USA) "Enteroviruses Transmission Through PS Vesicles" 7:50 pm - 7:55 pm Discussion 7:55 pm - 8:15 pm Mark Marsh (University College London, United Kingdom) "IFITM Proteins - Endosomal Broad-Spectrum Inhibitors of Virus Entry" 8:15 pm - 8:20 pm Discussion 8:20 pm - 8:40 pm Nels Elde (University of Utah, USA) "Adaptations Blocking Viral Exploitation of the ESCRT Pathway" 8:40 pm - 8:45 pm Discussion 8:45 pm - 9:05 pm Julie Magarian Blander (Icahn School of Medicine at Mount Sinai, USA) "Regulation of Vesicular Traffic for Meeting the Demands of Cross-Presentation" 9:05 pm - 9:10 pm Discussion 9:10 pm - 9:25 pm Shaeri Mukherjee (University of California, San Francisco, USA) "Bacterial Manipulation of the Host Unfolded Protein Response" 9:25 pm - 9:30 pm Discussion Tuesday 7:30 am - 8:30 am Breakfast 8:30 am Group Photo 9:00 am - 12:30 pm Dysregulation of Endosomal and Lysosomal Trafficking During Cancer and Other Pathologies Discussion Leaders: Alexander Sorkin (University of Pittsburgh School of Medicine, USA) and JoAnn Trejo (University of California, San Diego, USA) 9:00 am - 9:20 am Philippe Chavrier (Institut Curie, France) "Exocytosis from Late Endosomes Drives MT1-MMP Digest-on-Demand Response During Breast Cancer Invasion" 9:20 am - 9:30 am Discussion 9:30 am - 9:50 am Victor Faundez (Emory University, USA) "Genealogy Based Inference of Cellular Mechanisms in Complex Disorders" 9:50 am - 10:00 am Discussion 10:00 am - 10:15 am Doug Brooks (University of South Australia, Australia) "Altered Endosome Cell Biology in Prostate Cancer" 10:15 am - 10:20 am Discussion 10:20 am - 10:50 am Coffee Break 10:50 am - 11:10 am Rosalind Segal (Harvard Medical School / Dana-Farber Cancer Institute, USA) "Endocytosis and Signaling During Neurodegeneration" 11:10 am - 11:20 am Discussion 11:20 am - 11:40 am Alissa Weaver (Vanderbilt University School of Medicine, USA) "Regulation of Multivesicular Body Trafficking and Secretion in Cancer" 11:40 am - 11:50 am Discussion 11:50 am - 12:05 pm Chiara Zurzolo (Pasteur Institute, France) "Lysosomes Mediate the Transfer of Pathogenic Alpha Synuclein Fibrils Between Neurons in Tunneling Nanotubes (TNTs)" 12:05 pm - 12:10 pm Discussion 12:10 pm - 12:25 pm Florian Froehlich (University of Osnabrück, Germany) "Endosomal Trafficking in Sphingolipid Homeostasis and Its Implications in Neurodegenerative Disorders" 12:25 pm - 12:30 pm Discussion 12:30 pm Lunch 1:30 pm - 4:00 pm Free Time 4:00 pm - 6:00 pm Poster Session 6:00 pm Dinner 7:30 pm - 9:30 pm Metabolism and Related Diseases Discussion Leader: Frances Brodsky (University of California, San Francisco, USA / University College London, United Kingdom) 7:30 pm - 7:50 pm Andrea Ballabio (Telethon Institute of Genetics and Medicine, Italy) "Lysosome-to-Nucleus Signaling" 7:50 pm - 7:55 pm Discussion 7:55 pm - 8:15 pm Amira Klip (University of Toronto, Canada) "Insulin Transcytosis in Human Microvascular Endothelial Cells" 8:15 pm - 8:20 pm Discussion 8:20 pm - 8:40 pm Jennifer Lippincott-Schwartz (Janelia Research Campus, Howard Hughes Medical Institute, USA) "Mechanisms of Lipid Droplet Autophagy and Its Role in Yeast Lifespan Extension" 8:40 pm - 8:45 pm Discussion 8:45 pm - 9:05 pm Bao-Liang Song (Wuhan University, China) "Mechanisms of Intracellular Cholesterol Transport" 9:05 pm - 9:10 pm Discussion 9:10 pm - 9:25 pm Stephane Camus (University College London, United Kingdom) "CHC22 Clathrin Controls an Unconventional Secretory Pathway to Form the Insulin-Responsive GLUT4 Storage Compartment" 9:25 pm - 9:30 pm Discussion Wednesday 7:30 am - 8:30 am Breakfast 9:00 am - 12:30 pm Mechanisms of Lysosomal Degradation and Autophagy Discussion Leaders: Olivia Majer (University of California, Berkeley, USA) and Christian Ungermann (University of Osnabrück, Germany) 9:00 am - 9:20 am Markus Babst (University of Utah, USA) "Regulation of Nutrient Transporters by Lipid Domains" 9:20 am - 9:30 am Discussion 9:30 am - 9:50 am Vojo Deretic (University of New Mexico Health Sciences Center, USA) "Autophagy - Multiple Relationships with Lysosomes" 9:50 am - 10:00 am Discussion 10:00 am - 10:15 am Mark McNiven (Mayo Clinic, USA) "Lipophagic Regulation by Large and Small GTPases" 10:15 am - 10:20 am Discussion 10:20 am - 10:50 am Coffee Break 10:50 am - 11:10 am Haoxing Xu (University of Michigan, USA) "Ion Channels in the Lysosome: Opening the Gate to the Cell's Recycling Center" 11:10 am - 11:20 am Discussion 11:20 am - 11:40 am Richard Youle (National Institute of Neurological Disorders and Stroke, NIH, USA) "Downstream Steps of Mitophagy: Cargo-Centric Autophagosome Biogenesis and Lysosomal Fusion" 11:40 am - 11:50 am Discussion 11:50 am - 12:05 pm Phyllis Hanson (Washington University School of Medicine in St. Louis, USA) "New Functions for ESCRT-III?" 12:05 pm - 12:10 pm Discussion 12:10 pm - 12:25 pm Amy Kiger (University of California, San Diego, USA) "Rab2 Function in Autophagy and T-Tubule Membrane Remodeling" 12:25 pm - 12:30 pm Discussion 12:30 pm Lunch 1:30 pm - 4:00 pm Free Time 4:00 pm - 6:00 pm Poster Session 6:00 pm Dinner 7:00 pm - 7:30 pm Business Meeting Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair 7:30 pm - 9:30 pm Late-Breaking Topics Discussion Leaders: Tom Kirchhausen (Harvard Medical School, USA) and Lois Weisman (University of Michigan, USA) 7:30 pm - 7:50 pm Toyoshi Fujimoto (Nagoya University Graduate School of Medicine, Japan) "Lipids and Lipid Domains of the Yeast Vacuole" 7:50 pm - 8:00 pm Discussion 8:00 pm - 8:10 pm Short Talk Selected from Poster Abstracts 8:10 pm - 8:15 pm Discussion 8:15 pm - 8:30 pm Christophe Lamaze (Institut Curie, France) "EHD2 Controls Breast Cancer Aggressiveness Through Caveolae Mechanotransduction" 8:30 pm - 8:40 pm Discussion 8:40 pm - 8:55 pm Mary Munson (University of Massachusetts Medical School, USA) "Architecture of the Yeast Exocyst Complex" 8:55 pm - 9:05 pm Discussion 9:05 pm - 9:20 pm Chris Westlake (National Cancer Institute, NIH, USA) "Akt Regulates a Rab11-Effector Switch Required for Ciliogenesis Initiation" 9:20 pm - 9:30 pm Discussion Thursday 7:30 am - 8:30 am Breakfast 9:00 am - 12:30 pm Lysosomes, and Lysosome-Related Organelles Discussion Leaders: Robert Piper (University of Iowa, USA) and Angela Wandiger-Ness (University of New Mexico, USA) 9:00 am - 9:20 am Norma Andrews (University of Maryland, USA) "Lysosomes and Endocytosis as Agents of Plasma Membrane Repair" 9:20
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  • Study Shows Clathrin Protein Moonlights, Playing Key Role in Cell Division 6 September 2012
    Study shows clathrin protein moonlights, playing key role in cell division 6 September 2012 A protein called "clathrin," which is found in every tracks of structural proteins, and uses them as human cell and plays a critical role in transporting scaffolding to separate the cell's DNA (in the form materials within them, also plays a key role in cell of chromosomes) into two equal collections—one division, according to new research at the identical set of DNA for each of the new daughter University of California, San Francisco. cells. Scientists found that clathrin is involved in stabilizing these spindles. The discovery, featured on the cover of the Journal of Cell Biology in August, sheds light on the Now, however, Brodsky and her colleagues have process of cell division and provides a new angle shown that clathrin does even more. They deleted for understanding cancer. Without clathrin, cells clathrin from cells using a technique called RNA divide erratically and unevenly—a phenomenon that interference, which involves infusing in small is one of the hallmarks of the disease. genetic fragments that block the cell from making the clathrin. Doing so, Brodsky and her colleagues "Clathrin is doing more than we thought it was showed that clathrin stabilizes the structures in doing," said Frances Brodsky, DPhil, who led the dividing cells known as centrosomes. research. Brodsky is a professor in the UCSF Department of Bioengineering and Therapeutic Tagged with fluorescent chemicals and viewed Sciences, a joint department of the Schools of under a microscope, the centrosomes within a cell Pharmacy and Medicine, and she holds joint that is about to divide look like two glowing eyes appointments in Microbiology and Immunology, as peering through the dark.
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