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Damaris N. Lorenzo, Ph.D Damaris N. Lorenzo, Ph.D. Assistant Professor Department of Cell Biology and Physiology University of North Carolina-Chapel Hill, Chapel Hill, NC Email: [email protected] 5341C Medical Biomolecular Research Building Phone: (919) 966-6412 111 Mason Farm Road, School of Medicine Fax: (919) 966-6927 Chapel Hill, NC 27599-7545 _________________________________________________________________________________________________________________________________________________________________________________________________ Education 2004 − 2009 Ph.D. in Molecular, Cell, Developmental Biology and Genetics U. Minnesota Thesis title: Role of Spectrin Mutations in Spinocerebellar Ataxia Type 5 (SCA5). 1999 − 2003 M.Sc. in Plant Molecular Biology U. Havana Thesis title: In vitro propagation of Eucalyptus citriodora Hook. 1993 − 1999 B.S. in Biochemistry (Golden seal diploma (summa cum laude equivalent)) U. Havana Thesis title: Expression and purification of active antibody fragments specific . against the Hepatitis B Antigen in transgenic tobacco plants. Academic Appointments Jan 2018 – present Adjunct Professor, Department of Nutrition, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, NC. Sept 2016 – present Assistant Professor, Department of Cell Biology and Physiology, University of North Carolina-Chapel Hill, Chapel Hill, NC. Research 2009 – 2016 Postdoctoral fellow with Dr.Vann Bennett: Studying the roles of the membrane HHMI at Duke adaptor protein ankyrin- B in intracellular transport in neurons and other University cell types, and the molecular mechanism of the Ankyrin-B syndrome. 2005 − 2009 Ph.D. dissertation with Dr. Laura Ranum: Investigated the molecular mechanism U. Minnesota of spinocerebellar ataxia type 5 using human genetics and Drosophila approaches. 2004 – 2005 Rotations with professors Dr. Scott McIvor (gene therapy), Dr. Stephen C. U. Minnesota Jameson (immunology), and Dr. Laura Ranum (human genetics). 1999 − 2003 Master’s thesis: Developed novel approaches to micropropagate forest plants U. Havana of commercial interest. 1996 − 1999 Undergraduate honors thesis: Research at the Center for Genetic Engineering U. Havana Biotechnology on the expression and purification of active antibody fragments . specific for the Hepatitis B surface antigen in transgenic tobacco plants. Grants, Awards and Honors 2018 National Ataxia Foundation Young Investigator Award 2018 Junior Faculty Development Award (University of North Carolina-Chapel Hill) 2017 Keystone Symposia Early Career Investigator Travel Award 1 Damaris N. Lorenzo, Ph.D. 2016 American Society for Cell Biology Annual Meeting Faculty Travel Award 2016 Simmons Scholar (University of North Carolina-Chapel Hill) 2013 Keystone Symposia Scholarship 2012-15 First (2012) and Third (2013) places, and Honorable Mentions (2014-15) (postdoctoral) poster competition (ASCB conferences). 2007 − 2009 NIH/NINDS Ruth L. Kirschstein Predoctoral Fellowship. 2000 Annual Prize of the Cuban Academy of Sciences (awarded to the team who worked on the Expression of Active Antibody Fragments in Transgenic Tobacco Plants project). 1999 High impact thesis prize at the IX Forum of Science and Technology of the University of Havana (individual prize for highest impact undergraduate research). Teaching and Mentoring 2006 − present Trainer and mentor for two high school, five undergraduate, thirteen graduate, and two medical students. 2007 Teaching assistant for Genetics (Dr. Peter Snustad). Taught selected lectures U. Minnesota and discussion sections for undergraduate students. 2005 Teaching assistant for Cell Biology (Dr. Robert J. Brooker). Taught discussion U. Minnesota sections for undergraduate students. 2000- 2003 Lecturer for Plant Genetics. Prepared and taught lectures, discussion and U. Pinar del Río laboratory sections for undergraduates majoring in Forestry. Extramural Invited Presentations 03/12/2018 Lorenzo DN. "Regulation of glucose metabolism by ankyrin-B", University of Toronto, Invited by Dr. Amira Klip. 03/12/2018 Lorenzo DN. “Cell-autonomous obesity due to loss of ankyrin-B function”, Duke University, Developmental & Stem Cell Biology Colloquium 10/12/2017 Lorenzo DN. “Ankyrin-B and βII spectrin in axonal transport and brain connectivity” Université de Lyon. Invited by Dr. Thomas Boulin. 10/09/2017 Lorenzo DN. “Cell-autonomous adiposity due to ankyrin-B deficiency” University College London. Invited by Dr. Frances Brodsky. 03/29/2016 Lorenzo DN. “Anchored No More! Ankyrins as novel regulators of intracellular transport and metabolism” University of North Carolina-Chapel Hill. Invited by Dr. Kathleen Caron. 01/14/2016 Lorenzo DN. “Anchored No More! Ankyrins as novel regulators of intracellular transport and metabolism” University of Minnesota. Invited by Joseph Metzger. 12/15/2015 Lorenzo DN. “Anchored No More! Ankyrin‐B promotes organelle transport and axonal Elongation.” University of Florida. Invited by Dr. Laura Ranum. 03/24/2014 Lorenzo DN. “Ankyrin-B promotes axonal growth and multi-organelle transport” Genentech, San Francisco. Invited by Dr. Joseph Lewcock. Conference Presentations North Carolina Regional Diabetes Symposium “Ankyrins as Dual Membrane and Trafficking Adaptors: Molecular Mechanisms and Implications in Obesity and Diabetes”, Greensboro, NC, March 2018 2 Damaris N. Lorenzo, Ph.D. FASEB, “Regulation of GLUT4 endocytosis and adipogenesis by ankyrin-B”, Snowmass, CO, 07/2017 American Diabetes Association, “Ankyrin-B Regulates the Interplay between BCAA and Lipid Metabolism in Adipocytes”, San Diego, CA, June 2017 Experimental Biology 2017, “Regulation of Pancreatic Beta Cell Function by Ankyrin-B” Chicago, IL, April 2017 Annual Meeting of the Society for Neuroscience, “Independent and overlapping ankyrin-B- and β2- spectrin-based mechanisms control axonal organelle transport and development of long axonal tracts”, San Diego, CA, November 2016 Myotonic Dystrophy and the Brain: Brainstorming DM, “Anchored No More! Ankyrin-B Promotes Organelle Transport and Axonal Elongation” Gainesville, FL, December 2014 Memberships in Professional Societies 2016 – present American Diabetes Association 2008 – present Society for Neuroscience 2008 – present American Society for Cell Biology Professional Service Women in Science (WinS) Symposium, poster judge, 2018 CBP Department “Research Day”, poster judge, 2018 CBP Department Seminar Series Vetting Committee, chair, 2017 BBSP Graduate Admissions Committee, member, 2017-2018 CBP Faculty Search Committee, member, 2017-2018 CBP Department Seminar Series Vetting Committee, member, 2017 Job Search panel discussion, Duke University, panelist, 2017 External Thesis Reviewer, Åbo Akademi University, 2017 Mentoring and Career Development Round Table, panelist, Keystone Symposia, 2017 Manuscript reviewer: Frontiers in Neuroscience, 2016-present Molecular Biology of the Cell, 2016-present Poster judge, American Society for Cell Biology Annual Meeting, 2016 Publications Snouwaert, JN., Church R., Leigh Jania, Nguyen M., Wheeler M., Saintsing, A., Mieczkowski, P., de Villena, FPM., Armao, D., Moy, SS., Lorenzo DN., Koller, BH. A mutation in the Borcs7 subunit of the lysosome regulatory BORC complex results in motor deficits and dystrophic axonopathy in mice. Cell Reports. 2018. Under review. Lorenzo, DN; Bennett, V. Cell-autonomous adiposity through increased cell surface GLUT4 due to ankyrin-B deficiency. Proc. Natl. Acad. Sci. U.S.A. 2017. 2017. 14 (48) 12743-748. 3 Damaris N. Lorenzo, Ph.D. • Leong, I. Ankyrin-B in susceptibility to obesity Nature Reviews Endocrinology (Research Highlight). Published online 01 Dec 2017. Qu, F.; Lorenzo, DN; King, SJ; Brooks, R.; Bear, JE; Bennett, V. Ankyrin-B is a PI3P effector that promotes polarized α5β1-integrin recycling through recruiting RabGAP1L to early endosomes. eLIFE. 2016. 10: e20417. Bennett, V; Lorenzo, DN. 2016. An adaptable spectin/ankyrin-based mechanism for long-range organization of plasma membranes in vertebrate tissues. Curr Top Membr. 77:143–184. Lorenzo, DN.; Healy, JA; Hostettler, J; Davis, J; Yang, J; Wang, C; Hohmeier, HE; Zhang, M; Bennett, V. Ankyrin-B metabolic syndrome combines age-dependent adiposity with pancreatic β cell insufficiency. J Clin Invest. 2015. 125:3087-102. *Featured on JCI Impact. Lorenzo, DN; Badea, A; Davis, JQ; Hostettler J; He, J; Zhong, G; Zhuang, X; Bennett, V. 2014. A PIK3C3/Ankyrin-B/Dynactin pathway promotes axonal growth and multi-organelle transport. J Cell Biol, 2014. 207:735-52. Zhong, G; He, J; Ruobo, Z; Lorenzo, DN; Babcock, H; Bennett, V; Zhuang, X. Developmental mechanism of the periodic membrane skeleton in axons. eLIFE. 2014. 3: e04581. Bennett, V; Lorenzo, DN. Spectrin- and ankyrin-based membrane domains and the evolution of vertebrates. In V. Bennett (Ed.), Functional Organization of Vertebrate Plasma Membrane (1st edition, pp. 1-37). San Diego, US: Elsevier Science. 2013. Lorenzo, DN; Li, M; Mische, SE; Armbrust, KR; Ranum, LP; Hays, TS. Spectrin mutations which cause spinocerebellar ataxia type 5 impair axonal transport and induce neurodegeneration in Drosophila. J Cell Biol, 2010. 189(1): 143-158. Lorenzo, DN; Forrest, SM; Ikeda, Y; Dick, KA; Ranum, LP; Knight, MA. Spinocerebellar ataxia type 20 is genetically distinct from spinocerebellar ataxia type 5. Neurology. 2006. 67(11): 2084-5. Ramírez, N; Ayala, M; Lorenzo, DN; Palenzuela, D; Herrera, L; Doreste, V; Pérez, M.; Gavilondo, J.; Oramas, P. Expression of a single-chain Fv antibody fragment specific for the Hepatitis B Surface Antigen
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