Lysosomes & Endocytosis Gordon Research Conference Molecular Mechanisms and Functions of Endosomal and Lysosomal Pathways June 12-17, 2016 Proctor Academy Andover, NH Chair: Roberto Weigert Vice Chair: Lois S. Weisman Contributors Meeting Program Sunday 2:00 pm - 9:00 pm Arrival and Check-in 6:00 pm Dinner 7:30 pm - 7:40 pm Welcome / Introductory Comments by GRC Site Staff 7:40 pm - 9:30 pm Endocytic Machinery at the Plasma Membrane Discussion Leaders: Sandra Schmid (University of Texas Southwestern Medical Center, USA) and Mark Von Zastrow (University of California, San Francisco, USA) 7:40 pm - 8:00 pm Volker Haucke (Leibniz-Institut für Molekulare Pharmakologie, Germany) "Phosphoinositide Conversion Within the Endolysosomal System" 8:00 pm - 8:10 pm Discussion 8:10 pm - 8:30 pm Ludger Johannes (Institut Curie, France) "Using Glycosphingolipids to Build Endocytic Pits in Clathrin-Independent Endocytosis" 8:30 pm - 8:40 pm Discussion 8:40 pm - 9:00 pm Margaret Robinson (University of Cambridge, United Kingdom) "Coated Vesicle Adaptors" 9:00 pm - 9:10 pm Discussion 9:10 pm - 9:25 pm Sara Sigismund (IFOM, The FIRC Institute for Molecular Oncology Foundation, Italy) "ER-PM Contact Sites Control EGFR Endocytosis" 9:25 pm - 9:30 pm Discussion Monday 7:30 am - 8:30 am Breakfast 9:00 am - 12:30 pm Endosomal Sorting and Recycling Discussion Leaders: Elizabeth Conibear (University of British Columbia, Canada) and Scott Emr (Cornell University, USA) 9:00 am - 9:20 am Peter Cullen (University of Bristol, United Kingdom) "Molecular Mechanisms of Endosomal Cargo Sorting and Export" 9:20 am - 9:30 am Discussion 9:30 am - 9:50 am Julie Donaldson (National Heart, Lung, and Blood Institute, NIH, USA) "Sorting of Endosomal Cargo: Role of Cytoskeleton and Motors" 9:50 am - 10:00 am Discussion 10:00 am - 10:15 am Manojkumar Puthenveedu (Carnegie Mellon University, USA) "GPCR Localization and Signaling in Endosomal Recycling Microdomains" 10:15 am - 10:20 am Discussion 10:20 am - 10:45 am Coffee Break 10:45 am - 11:05 am Erik Jorgensen (Howard Hughes Medical Institute / University of Utah, USA) "Ultrafast Endocytosis of Synaptic Vesicles" 11:05 am - 11:15 am Discussion 11:15 am - 11:35 am Julie Brill (The Hospital for Sick Children, Canada) "Regulation of Endosomal Sorting and Recycling by PI4P" 11:35 am - 11:45 am Discussion 11:45 am - 12:05 pm Aitor Hierro (CIC bioGUNE, Spain) "Cargo-Specific Sorting from Endosomes" 12:05 pm - 12:15 pm Discussion 12:15 pm - 12:25 pm Zuzana Kadlecova (University of Texas Southwestern Medical Center, USA) "Hierarchical Allosteric Regulation of AP2 Activity Controls Clathrin-Mediated Endocytosis" 12:25 pm - 12:30 pm Discussion 12:30 pm Lunch 1:30 pm - 4:00 pm Free Time 4:00 pm - 6:00 pm Poster Session 6:00 pm Dinner 7:30 pm - 9:30 pm Exploiting the Endocytic Machinery During Host-Pathogen Interactions Discussion Leaders: Sergio Grinstein (Hospital for Sick Children, Canada) and Fabienne Paumet (Thomas Jefferson University, USA) 7:30 pm - 7:50 pm Nihal Altan-Bonnet (National Institutes of Health, USA) "Enteroviruses Transmission Through PS Vesicles" 7:50 pm - 7:55 pm Discussion 7:55 pm - 8:15 pm Mark Marsh (University College London, United Kingdom) "IFITM Proteins - Endosomal Broad-Spectrum Inhibitors of Virus Entry" 8:15 pm - 8:20 pm Discussion 8:20 pm - 8:40 pm Nels Elde (University of Utah, USA) "Adaptations Blocking Viral Exploitation of the ESCRT Pathway" 8:40 pm - 8:45 pm Discussion 8:45 pm - 9:05 pm Julie Magarian Blander (Icahn School of Medicine at Mount Sinai, USA) "Regulation of Vesicular Traffic for Meeting the Demands of Cross-Presentation" 9:05 pm - 9:10 pm Discussion 9:10 pm - 9:25 pm Shaeri Mukherjee (University of California, San Francisco, USA) "Bacterial Manipulation of the Host Unfolded Protein Response" 9:25 pm - 9:30 pm Discussion Tuesday 7:30 am - 8:30 am Breakfast 8:30 am Group Photo 9:00 am - 12:30 pm Dysregulation of Endosomal and Lysosomal Trafficking During Cancer and Other Pathologies Discussion Leaders: Alexander Sorkin (University of Pittsburgh School of Medicine, USA) and JoAnn Trejo (University of California, San Diego, USA) 9:00 am - 9:20 am Philippe Chavrier (Institut Curie, France) "Exocytosis from Late Endosomes Drives MT1-MMP Digest-on-Demand Response During Breast Cancer Invasion" 9:20 am - 9:30 am Discussion 9:30 am - 9:50 am Victor Faundez (Emory University, USA) "Genealogy Based Inference of Cellular Mechanisms in Complex Disorders" 9:50 am - 10:00 am Discussion 10:00 am - 10:15 am Doug Brooks (University of South Australia, Australia) "Altered Endosome Cell Biology in Prostate Cancer" 10:15 am - 10:20 am Discussion 10:20 am - 10:50 am Coffee Break 10:50 am - 11:10 am Rosalind Segal (Harvard Medical School / Dana-Farber Cancer Institute, USA) "Endocytosis and Signaling During Neurodegeneration" 11:10 am - 11:20 am Discussion 11:20 am - 11:40 am Alissa Weaver (Vanderbilt University School of Medicine, USA) "Regulation of Multivesicular Body Trafficking and Secretion in Cancer" 11:40 am - 11:50 am Discussion 11:50 am - 12:05 pm Chiara Zurzolo (Pasteur Institute, France) "Lysosomes Mediate the Transfer of Pathogenic Alpha Synuclein Fibrils Between Neurons in Tunneling Nanotubes (TNTs)" 12:05 pm - 12:10 pm Discussion 12:10 pm - 12:25 pm Florian Froehlich (University of Osnabrück, Germany) "Endosomal Trafficking in Sphingolipid Homeostasis and Its Implications in Neurodegenerative Disorders" 12:25 pm - 12:30 pm Discussion 12:30 pm Lunch 1:30 pm - 4:00 pm Free Time 4:00 pm - 6:00 pm Poster Session 6:00 pm Dinner 7:30 pm - 9:30 pm Metabolism and Related Diseases Discussion Leader: Frances Brodsky (University of California, San Francisco, USA / University College London, United Kingdom) 7:30 pm - 7:50 pm Andrea Ballabio (Telethon Institute of Genetics and Medicine, Italy) "Lysosome-to-Nucleus Signaling" 7:50 pm - 7:55 pm Discussion 7:55 pm - 8:15 pm Amira Klip (University of Toronto, Canada) "Insulin Transcytosis in Human Microvascular Endothelial Cells" 8:15 pm - 8:20 pm Discussion 8:20 pm - 8:40 pm Jennifer Lippincott-Schwartz (Janelia Research Campus, Howard Hughes Medical Institute, USA) "Mechanisms of Lipid Droplet Autophagy and Its Role in Yeast Lifespan Extension" 8:40 pm - 8:45 pm Discussion 8:45 pm - 9:05 pm Bao-Liang Song (Wuhan University, China) "Mechanisms of Intracellular Cholesterol Transport" 9:05 pm - 9:10 pm Discussion 9:10 pm - 9:25 pm Stephane Camus (University College London, United Kingdom) "CHC22 Clathrin Controls an Unconventional Secretory Pathway to Form the Insulin-Responsive GLUT4 Storage Compartment" 9:25 pm - 9:30 pm Discussion Wednesday 7:30 am - 8:30 am Breakfast 9:00 am - 12:30 pm Mechanisms of Lysosomal Degradation and Autophagy Discussion Leaders: Olivia Majer (University of California, Berkeley, USA) and Christian Ungermann (University of Osnabrück, Germany) 9:00 am - 9:20 am Markus Babst (University of Utah, USA) "Regulation of Nutrient Transporters by Lipid Domains" 9:20 am - 9:30 am Discussion 9:30 am - 9:50 am Vojo Deretic (University of New Mexico Health Sciences Center, USA) "Autophagy - Multiple Relationships with Lysosomes" 9:50 am - 10:00 am Discussion 10:00 am - 10:15 am Mark McNiven (Mayo Clinic, USA) "Lipophagic Regulation by Large and Small GTPases" 10:15 am - 10:20 am Discussion 10:20 am - 10:50 am Coffee Break 10:50 am - 11:10 am Haoxing Xu (University of Michigan, USA) "Ion Channels in the Lysosome: Opening the Gate to the Cell's Recycling Center" 11:10 am - 11:20 am Discussion 11:20 am - 11:40 am Richard Youle (National Institute of Neurological Disorders and Stroke, NIH, USA) "Downstream Steps of Mitophagy: Cargo-Centric Autophagosome Biogenesis and Lysosomal Fusion" 11:40 am - 11:50 am Discussion 11:50 am - 12:05 pm Phyllis Hanson (Washington University School of Medicine in St. Louis, USA) "New Functions for ESCRT-III?" 12:05 pm - 12:10 pm Discussion 12:10 pm - 12:25 pm Amy Kiger (University of California, San Diego, USA) "Rab2 Function in Autophagy and T-Tubule Membrane Remodeling" 12:25 pm - 12:30 pm Discussion 12:30 pm Lunch 1:30 pm - 4:00 pm Free Time 4:00 pm - 6:00 pm Poster Session 6:00 pm Dinner 7:00 pm - 7:30 pm Business Meeting Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair 7:30 pm - 9:30 pm Late-Breaking Topics Discussion Leaders: Tom Kirchhausen (Harvard Medical School, USA) and Lois Weisman (University of Michigan, USA) 7:30 pm - 7:50 pm Toyoshi Fujimoto (Nagoya University Graduate School of Medicine, Japan) "Lipids and Lipid Domains of the Yeast Vacuole" 7:50 pm - 8:00 pm Discussion 8:00 pm - 8:10 pm Short Talk Selected from Poster Abstracts 8:10 pm - 8:15 pm Discussion 8:15 pm - 8:30 pm Christophe Lamaze (Institut Curie, France) "EHD2 Controls Breast Cancer Aggressiveness Through Caveolae Mechanotransduction" 8:30 pm - 8:40 pm Discussion 8:40 pm - 8:55 pm Mary Munson (University of Massachusetts Medical School, USA) "Architecture of the Yeast Exocyst Complex" 8:55 pm - 9:05 pm Discussion 9:05 pm - 9:20 pm Chris Westlake (National Cancer Institute, NIH, USA) "Akt Regulates a Rab11-Effector Switch Required for Ciliogenesis Initiation" 9:20 pm - 9:30 pm Discussion Thursday 7:30 am - 8:30 am Breakfast 9:00 am - 12:30 pm Lysosomes, and Lysosome-Related Organelles Discussion Leaders: Robert Piper (University of Iowa, USA) and Angela Wandiger-Ness (University of New Mexico, USA) 9:00 am - 9:20 am Norma Andrews (University of Maryland, USA) "Lysosomes and Endocytosis as Agents of Plasma Membrane Repair" 9:20