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Histopathology and Laboratory Features of Sexually Transmitted Diseases
Histopath & Labs for STIs Endo, Energy and Repro 2017-2018 HISTOPATHOLOGY AND LABORATORY FEATURES OF SEXUALLY TRANSMITTED DISEASES Dominck Cavuoti, D.O. Phone: 469-419-3412 Email: [email protected] LEARNING OBJECTIVES: • Identify the etiologic agents causing pelvic inflammatory disease and the pathologic changes they produce. • Discuss the characteristic clinical and pathologic findings caused by herpes simplex virus (HSV) infections: a. fever blisters b. genital herpes simplex virus infection c. disseminated neonatal HSV • Describe the pathologic changes produced by Treponema pallidum. • Describe the clinical features and pathologic changes produced by Chlamydia trachomatisand Neisseria gonorrhoeae • Describe the clinical and laboratory features of vaginal infections including: Trichomonas, Candida, and bacterial vaginosis. • Describe the clinical and laboratory features of ectoparasite infections PURPOSE OF THE LECTURE: 1. To describe the various agents of sexually transmitted diseases and their disease manifestations 2. To describe the pathologic features associated with STDs 3. To introduce some of the laboratory aspects of STDs TERMS INTRODUCED IN LECTURE: Condyloma lata Disseminated gonococcal infection Gummatous syphilis Lymphogranuloma venereum Pelvic inflammatory disease Rapid Plasma Reagin (RPR) Salpingitis Syphilis/endarteritis obliterans Venereal Disease Research Laboratory (VDRL) Treponema pallidum particle agglutination (TPPA) Histopath & Labs for STIs Endo, Energy and Repro 2017-2018 MAJOR CONCEPTS EMPHASIZED IN LECTURE I. Syphilis (Will be covered by Dr. Norgard in later lecture). II. Gonorrhea A. Causative agent: Neisseria gonorrhoeae, a Gram negative diplococcus. Humans are the only natural reservoir. Infection is acquired via direct contact with the mucosa of an infected person. The incubation period averages 2-5 days with a range of 1-14 days. -
Infection Control in Dentistry: How to Asepsis Photographic Mirrors?
Infection control in dentistry: how to asepsis photographic mirrors? Amanda Osório Ayres de Freitas* Mariana Marquezan* Giselle Naback Lemes Vilani* Rodrigo César Santiago* Luiz Felipe de Miranda Costa* Sandra Regina Torres** Abstract: The aim of this study was to evaluate the efficacy of six different methods of disinfection and sterilization of intraoral photographic mirrors through microbiological testing and to analysis their potential harm to mirrors’ surface. Fourteen occlusal mirrors were divided into seven groups. Group 1 comprised two mirrors as received from manufacturer. The other six groups comprised mirrors disinfected/sterilized by autoclave, immersion in enzymatic detergent, and friction with chlorhexidine detergent, chlorhexidine wipes, common detergent and 70% ethylic alcohol. Microbiological and quality surface analyses were performed. Sterilization in autoclave was microbiologic effective, but caused damage to the mirror surface. Chlorhexidine (in wipes or detergent) and liquid soap were effective disinfectant agents for photographic mirrors decontamination, without harmful effect on its surface. Enzymatic detergent immersion and friction with 70% ethylic alcohol were not effective as disinfectant agents for photographic mirrors decontamination. According to the results, the more effective and safe methods for photographic mirrors disinfection were friction with chlorhexidine wipes or detergent, as well as liquid soap. Results, the most efficacious methods for photographic mirrors disinfection were friction with chlorhexidine wipes and detergent, as well as common detergent. Descriptors: Dental Instruments; Decontamination; Microbiology; Surface Properties. *Doutoranda em Odontologia na Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brasil **Pósdoutora em odontologia pela University of Washington (UW), Seattle, WA, Estados Unidos ISSN 22365843 │ 93 Introduction Dental photography is an important tool for diagnostic and treatment planning, and it’s also a registration of the patient’s condition before and after treatment. -
ANTT Guidelines
www.antt.org ANTT Guidelines The ANTT Clinical Guideline for the Preparation & Administration of Peripheral and Central Intravenous Medications (IV Therapy) Rationale and supporting evidence ANTT IV Prep and Administration V3 .0 2013 The Association for Safe Aseptic Technique (ASAP) www.antt.org www.antt .org ® © 2013 Aseptic Non Touch Technique (ANTT) This document is a publication of The-ASAP and all rights of copyright, intellectual property and Trademark are reserved. ANTT is protected to prevent dilution and misrepresentation of the practice framework so as to avoid ANTT becoming another unhelpful generic term for aseptic technique that is variably interpreted. For guidance see [email protected]. This document may however, be freely reviewed, copied and translated, in part, or in whole, for LOCAL, SINGLE ORGANIZATION educational use. It must not be published via the www/internet or its content used for production and publication of dedicated ANTT e-learning resources. ANTT is not for sale or for use in conjunction with commercial purposes. The-ASAP provide a number of free core ANTT resources to help disseminate and train healthcare staff. The- ASAP requests that the balance it determines between free dissemination and protection of the standard is respected in the interests of patient safety. Disclaimer: The-ASAP provides the ANTT Clinical Practice Framework and ANTT Clinical Guidelines to healthcare organizations in good faith in a collaboration to promote effective aseptic technique. It is the responsibility of healthcare organizations to implement ANTT effectively. No guarantee or responsibility for the application or outcome of clinical practice can be, or is, assumed or accepted by The-ASAP/ANTT. -
Medical Laboratory Assistant, a Suggested Guide for A
p .7/ tWI .7- y,1.. P O R T R ESUM ED 013 321 VT 002 2L4 MEDICAL LABORATORY ASSISTANT, A SUGGESTEDGUIDE FOR A TRAINING PROGRAM. OFFICE OF EDUCATION, WASHINGTON, D.C. REPORT NUMBER 0E-87017 PUB DATE 66 EDRS PRICE MF-$0.50 HC-$4.92 123P. DESCRIPTORS-. *MEDICAL LABORATORY ASSISTANTS,TEACHING GUIDES, *PROGRAM PLANNING, PROGRAM DEVELOPMENT,*CURRICULUM GUIDES, CURRICULUM, *HEALTH OCCUPATIONSEDUCATION, POST SECONDARY EDUCATION, MDTA PROGRAMS, INFORMATION IS GIVEN TO ASSIST IN ORGANIZINGAND ADMINISTERING A TRAINING PROGRAM FOR MEDICAL LABORATORY ASSISTANTS IN A VARIETY OF SETTINGS AND TO PROVIDEGUIDANCE IN ESTABLISHING NEW PROGRAMS AND IN EVALUATINGEXISTING ONES. THE MATERIAL WAS PREPARED UNDER THE DIRECTIONOF THE NATIONAL COMMITTEE =OR CAREERS IN MEDICAL TECHNOLOGY.PATHOLOGISTS AND MEDICAL TECHNOLOGISTS PARTICIPATEDIN THE ORGANIZATIONAL AND DEVELOPMENTAL STAGES. ALL MATERIAL WAS REVIEWEDBY A REPRESENTATIVE NATIONAL GROUP OF EXPERTCONSULTANTS IN THE FIELD OF LABORATORY MEDICINE. THE 12-MONTH PROGRAM WAS DESIGNED FOR HIGH SCHOOL GRADUATES OR THEIREQUIVALENT TO SE ADMINISTERED BY A TEACHING STAFF COMPOSEDOF A NATIONAL DIRECTOR, A. TEACHING SUPERVISOR, ANDINSTRUCTORS. AN OUTLINE OF INFORMATIONAL MATERIAL TO BE PRESENTEDIN THE CLASSROOM, LABORATORY PROCEDURES TO BE DEMONSTRATEDAND THEN PERFORMED AS DIRECT EXERCISES BY THE STUDENTS, AS WELLAS RELEVANT BIBLIOGRAPHIES, AUDIOVISUAL AIDS, AND STUDYQUESTIONS ARE PRESENTED FOR THE FOLLOWING UNITS (1) ORIENTATION TO THE CLINICAL LABORATORY,(2) BACTERIOLOGY,(3) SEROLOGY, (4) PARASITOLOGY,(5) HEMATOLOGY, (6) CLINICAL CHEMISTRY,(7) BLOOD BANKING,(8) ROUTINE ANALYSIS, AND (9) BASAL METABOLISM -- ELECTROCARDIOGRAPHY. THIS DOCUMENT IS AVAILABLE AS GPO NUMBER FS 5.267-07017 FOR 60 CENTS FROMSUPERINTENDENT OF DOCUMENTS, U.S. GOVERNMENT PRINTING OFFICE,WASHINGTON, D.C. 20402. (PS) cir 02281"2=10. -
Francisella Tularensis 6/06 Tularemia Is a Commonly Acquired Laboratory Colony Morphology Infection; All Work on Suspect F
Francisella tularensis 6/06 Tularemia is a commonly acquired laboratory Colony Morphology infection; all work on suspect F. tularensis cultures .Aerobic, fastidious, requires cysteine for growth should be performed at minimum under BSL2 .Grows poorly on Blood Agar (BA) conditions with BSL3 practices. .Chocolate Agar (CA): tiny, grey-white, opaque A colonies, 1-2 mm ≥48hr B .Cysteine Heart Agar (CHA): greenish-blue colonies, 2-4 mm ≥48h .Colonies are butyrous and smooth Gram Stain .Tiny, 0.2–0.7 μm pleomorphic, poorly stained gram-negative coccobacilli .Mostly single cells Growth on BA (A) 48 h, (B) 72 h Biochemical/Test Reactions .Oxidase: Negative A B .Catalase: Weak positive .Urease: Negative Additional Information .Can be misidentified as: Haemophilus influenzae, Actinobacillus spp. by automated ID systems .Infective Dose: 10 colony forming units Biosafety Level 3 agent (once Francisella tularensis is . Growth on CA (A) 48 h, (B) 72 h suspected, work should only be done in a certified Class II Biosafety Cabinet) .Transmission: Inhalation, insect bite, contact with tissues or bodily fluids of infected animals .Contagious: No Acceptable Specimen Types .Tissue biopsy .Whole blood: 5-10 ml blood in EDTA, and/or Inoculated blood culture bottle Swab of lesion in transport media . Gram stain Sentinel Laboratory Rule-Out of Francisella tularensis Oxidase Little to no growth on BA >48 h Small, grey-white opaque colonies on CA after ≥48 h at 35/37ºC Positive Weak Negative Positive Catalase Tiny, pleomorphic, faintly stained, gram-negative coccobacilli (red, round, and random) Perform all additional work in a certified Class II Positive Biosafety Cabinet Weak Negative Positive *Oxidase: Negative Urease *Catalase: Weak positive *Urease: Negative *Oxidase, Catalase, and Urease: Appearances of test results are not agent-specific. -
Biofire Blood Culture Identification System (BCID) Fact Sheet
BioFire Blood Culture Identification System (BCID) Fact Sheet What is BioFire BioFire BCID is a multiplex polymerase chain reaction (PCR) test designed to BCID? identify 24 different microorganism targets and three antibiotic resistance genes from positive blood culture bottles. What is the purpose The purpose of BCID is to rapidly identify common microorganisms and of BCID? antibiotic resistance genes from positive blood cultures so that antimicrobial therapy can be quickly optimized by the physician and the antibiotic stewardship pharmacist. It is anticipated that this will result in improved patient outcomes, decreased length of stay, improved antibiotic stewardship, and decreased costs. When will BCID be BCID is performed on all initially positive blood cultures after the gram stain is routinely performed and reported. performed? When will BCID not For blood cultures on the same patient that subsequently become positive with be routinely a microorganism showing the same morphology as the initial positive blood performed? culture, BCID will not be performed. BCID will not be performed on positive blood cultures with gram positive bacilli unless Listeria is suspected. BCID will not be performed on blood culture bottles > 8 hours after becoming positive. BCID will not be performed between 10PM-7AM on weekdays and 2PM-7AM on weekends. BCID will not be performed for clinics that have specifically opted out of testing. How soon will BCID After the blood culture becomes positive and the gram stain is performed and results be available? reported, the bottle will be sent to the core Microbiology lab by routine courier. BCID testing will then be performed. It is anticipated that total turnaround time will generally be 2-3 hours after the gram stain is reported. -
Module 6: Principles of Asepsis
Module 6: Medical and Surgical Asepsis Module 6: Medical and Surgical Asepsis Minimum Number of Theory Hours: 2 Suggested Theory Hours: 5 Recommended Clinical Hours: 8 Statement of Purpose: The purpose of this unit is to present information about asepsis and the control of infection. Procedures and precautions to protect patient/patients/residents, health care workers and others from infection are presented, including standard precautions, transmission- based precautions and biohazardous waste management. Terminology 1. Acquired Immunodeficiency 21. Escherichia coli (E. coli) 40. Non-intact Syndrome (AIDS) 22. Excretions 41. Nosocomial 2. Airborne precautions 23. Exposure incident 42. Occupational Safety and Health 3. Asepsis 24. Flora Administration (OSHA) 4. Athlete’s foot 25. Fungus 43. Pathogens 5. Bacteria 26. Health Care-Associated Infection 44. Personal Protective Equipment 6. Barriers (HAI) (PPE) 7. Biohazard symbol 27. Hepatitis A, B, C, D, E 45. Pneumonia 8. Bloodborne 28. Herpes zoster 46. Precautions 9. Carrier spore 29. Host 47. Protozoa 10. Centers for Disease Control (CDC) 30. Immunity 48. Reservoir 11. Chain of infection 31. Infection 49. Reverse isolation 12. Communicable 32. Infectious agent 50. Rickettsia 13. Contact precautions 33. Influenza 51. Scabies 14. Contagious microbes 34. Isolation 52. Sepsis 15. Contamination 35. Lice 53. Standard precautions 16. Disinfection 36. Material Safety Data Sheet (MSDS) 54. Sterilization 17. Disorientation 37. Methicillin-Resistant 55. Streptococcus 18. Disposable Staphylococcus -
The Efficacy of Three Hand Asepsis Techniques Using Chlorhexidine Gluconate (Chg 2%)
A RTIGO Eficácia de três métodos de degermação das mãos utilizando gluconato de clorexidina O degermante (GCH 2%) RIGINAL THE EFFICACY OF THREE HAND ASEPSIS TECHNIQUES USING CHLORHEXIDINE GLUCONATE (CHG 2%) EFICACIA DE TRES MÉTODOS DE DESINFECCIÓN DE LAS MANOS UTILIZANDO GLUCONATO DE CLORHEXIDINA ANTISÉPTICA (GHC 2%) Érika Rossetto da Cunha1, Fabiana Gonçalves de Oliveira Azevedo Matos2, Adriana Maria da Silva3, Eutália Aparecida Cândido de Araújo4, Karine Azevedo São Leão Ferreira5, Kazuko Uchikawa Graziano6 RESUMO ABSTRACT RESUMEN A degermação cirúrgica das mãos e dos The scrubbing of hands and forearms using La desinfección quirúrgica de manos y an- antebraços é um procedimento que inte- anti septi c agents has been the standard pre- tebrazos es un procedimiento que integra gra as ati vidades de paramentação cirúr- operati ve procedure to prevent surgical site las acti vidades prequirúrgicas como me- gica como uma medida de prevenção de infecti on. With the introducti on of anti sep- dida de prevención contra infección del infecção do síti o cirúrgico. Com o advento ti c agents, the need to use brushes for pre- siti o quirúrgico. Con el advenimiento de la dos princípios anti ssépti cos degermantes, operati ve disinfecti on has been questi oned anti sepsia desinfectante, se cuesti ona y se a necessidade do uso de escovas para a and it has been recommended that the pro- recomienda dejar de lado el uso de cepillos degermação cirúrgica tem sido questi ona- cedure be abandoned due to the injuries it debido a lesiones provocadas en piel. Para da e recomendado o abandono deste uso may cause to the skin. -
Interpreting and Acting on Positive Blood Cultures Trevor Van Schooneveld, MD 1/18/18 Objectives
Stewardship Interventions: Interpreting and Acting on Positive Blood Cultures Trevor Van Schooneveld, MD 1/18/18 Objectives • Interpret the results of blood cultures including gram stains and rapid pathogen diagnostic tests • Make recommendations regarding antimicrobial therapy based on interpretation of blood culture data Early Initiation of Active Therapy is Essential Predicted hospital mortality and 95% CIs for time to first antibiotic administration Surviving Sepsis Guidelines (N=28,150 severe sepsis, septic shock patients) • Administer IV antimicrobials within one hour of presentation (strong) • Initiate empiric, broad-spectrum therapy with one or more agents to cover all likely pathogens (strong) Ferrer R, et al. Crit Care Med. 2014;42:1749-55. Rhodes A, et al. Crit Care Med. 2017;45:486-552. De-escalation Also is Important Surviving Sepsis Guidelines • Narrow empiric antibiotics once pathogen identified and/or clinical improvement De-escalation Benefit • De-escalation in severe sepsis, septic shock (N=712) • Mortality OR 0.54 (95% CI 0.33-0.89, P=.016) • De-escalation in community-onset gram-negative bacteremia (N=189) • Mortality OR 0.37 (0.14-0.96, P=.04) Garnarcho-Montero J, et al. Intensive Care Med. 2014;40:32-40. Lee C, et al. Diag Micro Infect Dis. 2015;82:158-64. Issues with Treatment of Sepsis/Bacteremia Under-treatment • May die (mortality) • May not get better as quickly (LOS, cost) • May develop complications (LOS, cost) Overtreatment • May develop toxicities (cost, LOS) • May develop C. difficile (cost, LOS, readmission) -
BLOOD CULTURE MEDIA Principle: Specimen: Reagent Preparation Storage: Procedure
BLOOD CULTURE MEDIA For In-Vitro and professional use only Store at (2° to 8°C) Blood cultures are used to detect the presence of bacteria or fungi in the blood, to identify the type present, and to guide treatment. Testing is used to identify a blood infection (septicemia) that can lead to sepsis, a serious and life-threatening complication. Individuals with a suspected blood infection are often treated in intensive care units, so testing is often done in a hospital setting. A bacterial infection in the blood called bacteremia. It can be serious because the blood can spread the bacteria to any part of the body. Blood infections most often occur with other serious infections such as those affecting the lungs, kidneys, bowel, gallbladder, or heart valves. Blood infections may also develop when the immune system is weak in infants and older adults, from disease (such as cancer or AIDS) or from medicines (such as corticosteroids or chemotherapy) that change the ability of your body to fight infections (immunity). Principle: The vials containing 25 ml or 50 ml of brain heart infusion, yeast extract, SPS and other stabilizers. The Media is used for yeast, aerobic and anaerobic organisms in blood. The principle of the this test is that each type of organisms need a certain time to grow and multiply. Specimen: Blood. Reagent preparation The vials are ready to use. Storage: Store reagent from (2 - 8 oC). Procedure: 1. Bottles of Brain Heart Infusion which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen , and cooled rapidly without shaking , just before use. -
Summary ANTIMICROBIAL AGENTS
ANTIMICROBIAL AGENTS Summary of a Round Table Discussion By Mark H. Lepper, M.D., and Harris D. Riley, Jr., M.D. Department of Preventive Medicine, University of Illinois (M.H.L.), and Department of Pediatrics, University of Oklahoma (H.D.R.) NTIMKROBIAL agents have had an espe- sistance of the host to infection. (The effect cially great impact in pediatrics. Al- of cortisone on stneptococcal infections in though many diseases have been conquered the rabbit was cited : 58 out of 66 rabbits easily with proper antimicrobial therapy, pnetreated with cortisone died; 5 of 60 con- there still remain difficulties and failures. trol animals died.) Instances of empyema Dr. Leppen opened the session with a dis- developing during treatment of pneuimo- cussion of some of the failures of antimi- coccal pneumonia with both antibiotics and crobial therapy. ACTH were described. The discussants agreed that at no time should adrenal con- HYPERACUTE INFECTIONS ticosteroids be used in the treatment of in- Hyperacute infections, i.e., infections fectious processes without simultaneoums ad- which are often fatal within 24 hours from ministration of adequate amounts of appro- the onset of symptoms, and resistant strains pniate antibiotics. of organisms, account for the vast majority Dr. Lepper reviewed some of the salient of failures in the use of antibiotics. A few facts in connection with the use of cortisone cases cannot be classified into either cate- and allied substances in the treatment of gory and remain as unexplained failures. overwhelming infections, including menin- The magnitude of the problem of hypen- gococcemia and the Waterhouse-Fnidenich- acute infections can be judged by the fact sen syndrome. -
El Paso Community College Syllabus Part II Official Course Description
MLAB 2434; Revised Fall 2019/Spring 2020 El Paso Community College Syllabus Part II Official Course Description SUBJECT AREA Medical Laboratory Technology COURSE RUBRIC AND NUMBER MLAB 2434 COURSE TITLE Clinical Microbiology COURSE CREDIT HOURS 4 3 : 4 Credits Lec Lab I. Catalog Description Provides instruction in the theory, practical application, and pathogenesis of clinical microbiology, including collection, quality control, quality assurance, lab safety, setup, identification, susceptibility testing, and reporting results. A grade of "C" or better is required in this course to take the next course. Corequisite: MLAB 2360. (3:4). Lab fee. II. Course Objectives A. Unit I. Introduction to Medical Microbiology 1. State general safety rules for handling pathogenic cultures and potentially infective clinical specimens, to include: a. the importance of personal cleanliness, frequent handwashing, and not putting pens and pencils in the mouth. b. the importance of wearing a lab coat to protect clothing from contamination. c. the importance of keeping personal articles such as books, coats, handbags, etc., off of bench areas. d. reporting all spills of infectious materials and the proper method of cleaning up infectious spills. e. reporting all spills of hazardous materials and the proper method of cleaning up hazardous spills. f. the importance of not eating, drinking, smoking, or applying cosmetics in the laboratory. g. the importance of not wearing open sandals in the laboratory. h. the importance of proper trash disposal--separating paper trash from infectious materials and disposing of infectious materials only in specially marked biohazard bags and paper trash in the regular trash cans. i. reporting any cut or accident involving infectious material, no matter how small.