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Usbiological Datasheet HIST1H2AG, CT (HIST1H2AG, H2AFP, Histone H2A type 1, Histone H2A/p) Catalog number 036588 Supplier United States Biological Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. Applications Suitable for use in Western Blot. Other applications not tested Recommended Dilutions Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Immunogen KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa102-130 in the C-terminal region of human HIST1H2AG, UniProt Accession #NP_066408.1; P0C0S8. Species Sequence Homology: bovine, mouse and rat Formulation Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity Purified by Protein A affinity chromatography. Specificity Recognizes human HIST1H2AG at ~14kD. Product Type Pab Source human Isotype IgG Grade Affinity Purified Applications WB Crossreactivity Hu Storage -20°C Reference 1. Shi, J., et al. Nature 460(7256):753-757(2009). 2. Nicassio, F., et al. Curr. Biol. 17(22):1972-1977(2007). 3. Lusic, M., et al. EMBO J. 22(24): 6550-6561(2003). 3. Marzluff, W.F., et al. Genomics 80(5):487-498(2002). 4. Deng, L., et al. Virology 289(2):312-326(2001) Powered by TCPDF (www.tcpdf.org).
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