Fucus Serratus Linneaus Aqueous Extracts and Examination of the Routes of Uptake of Minerals Both in Vivo and in Vitro
Total Page:16
File Type:pdf, Size:1020Kb
Investigation of the mineral profile of Fucus serratus Linneaus aqueous extracts and examination of the routes of uptake of minerals both in vivo and in vitro Author Tarha Westby Supervisors Dr. Aodhmar Cadogan Ms. Geraldine Duignan Submitted for the award of Doctor of Philosophy at Institute of Technology Sligo Table of Contents Abstract .............................................................................................................................. 8 Declaration ......................................................................................................................... 9 Acknowledgements .......................................................................................................... 10 List of abbreviations ......................................................................................................... 12 1 Introduction ........................................................................................................... 14 Seaweed Baths: an underexplored resource ..................................................................... 14 1.1 Seaweed and the Global Context ......................................................................... 17 1.1.2 Agriculture ..................................................................................................... 22 1.1.3 Algin isolation................................................................................................ 22 1.1.4 Bioactive isolation ......................................................................................... 23 1.1.5 Biofuel ........................................................................................................... 25 1.1.6 Food ............................................................................................................... 25 1.1.7 Seaweed Baths ............................................................................................... 26 1.2 Seaweed................................................................................................................. 28 1.2.1 Seaweed composition .................................................................................... 29 1.2.2 Fibre ............................................................................................................... 29 1.2.3 Carbohydrate .................................................................................................. 29 1.2.4 Protein ............................................................................................................ 30 1.2.5 Lipid ............................................................................................................... 30 1.2.6 Vitamin and Mineral ...................................................................................... 30 1.3 Brown seaweed ..................................................................................................... 33 1.4 Iodine..................................................................................................................... 38 1.4.1 Recommended Daily Allowance (RDA) ....................................................... 41 1.4.2 Sources ........................................................................................................... 42 2 1.4.3 Health Impact ................................................................................................. 43 1.4.4 Deficiency and Excess ................................................................................... 44 1.4.5 Urinary Iodine (UI) ........................................................................................ 45 1.5 Other minerals of interest ...................................................................................... 46 1.6 Skin Architecture .................................................................................................. 51 1.6.1 Epidermis ....................................................................................................... 51 1.7 Analytical Methods ............................................................................................... 54 1.7.1 Sandell-Kolthoff (SK) method....................................................................... 54 1.7.2 Spectroscopy .................................................................................................. 55 1.7.3 Inductively Coupled Plasma Mass Spectrometry (ICP-MS) ......................... 57 1.7.4 Enhancer cells ................................................................................................ 59 1.8 Potential for dermal absorption from seaweed bathwater ..................................... 62 1.9 Problem statement ................................................................................................. 63 1.10 Justification ........................................................................................................... 64 1.11 Hypothesis ............................................................................................................. 64 1.12 Objectives .............................................................................................................. 65 1.12.1 General objective ........................................................................................... 65 1.12.2 Specific objectives ......................................................................................... 65 2 Methodology: Materials and Instrumentation ....................................................... 67 2.1 Choice of Analytical Methods .............................................................................. 67 2.2 Materials ................................................................................................................ 71 2.3 Optimization and validation of the SK micromethod for total iodine determination in Fucus serratus L. seaweed, seawater, bathwater and urine ......................................... 72 2.3.1 SK method reagent preparation ..................................................................... 72 2.3.2 SK method optimization ................................................................................ 72 3 2.3.3 SK micromethod ............................................................................................ 73 2.3.4 SK method validations ................................................................................... 74 2.3.5 SK method optimization ................................................................................ 75 2.3.6 SK micromethod validation ........................................................................... 80 2.3.7 Specificity: Alginate interference .................................................................. 88 2.3.8 Correlation between methods ........................................................................ 87 2.3.9 Comparison of methods ................................................................................. 88 2.3.10 UIC .................................................................................................................... 89 2.4 Seaweed sample collection, preparation, homogeneity studies, stability and pH procedures ........................................................................................................................ 90 2.4.1 Sample collection ........................................................................................... 90 Microwave, AAS and FES optimization and ICP-MS optimization ............................... 91 Dermal absorption in vitro ............................................................................................... 91 2.4.2 Simulated Bath preparation ........................................................................... 91 AAS, FES, ICP-MS and Dermal absorption/penetration in vitro .................................... 92 2.4.3 Sample homogeneity...................................................................................... 92 2.4.4 Sample stability.............................................................................................. 94 2.4.4.1 Long term stability ..................................................................................... 94 2.4.4.2 Freeze Thaw stability ................................................................................. 95 2.4.5 Sample pH...................................................................................................... 96 2.5 Optimization of ICP-MS for determination of aluminium, cadmium, chromium, cobalt, copper, lead, manganese, molybdenum, nickel, selenium and zinc in Fucus serratus L. seaweed, seawater and bathwater. ................................................................. 97 2.5.1 Microwave digestion optimization ................................................................ 97 2.5.2 ICP-MS set up ................................................................................................ 99 4 2.5.3 ICP-MS optimization ........................................................................................ 99 2.6 Optimization of Atomic Absorption Spectrophotometry (AAS) and Flame Emission Spectrophotometry (FES) methods for determination of calcium, magnesium, potassium and sodium in Fucus serratus L. seaweed, seawater and bathwater ............ 104 2.6.1 AAS optimization ........................................................................................ 104 2.6.2 FES optimization ......................................................................................... 106 2.7 Optimization of Enhancer cell technology