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paranoia, hallucinations, and psychotic behavior. The effects of generally last 2-4 unconsciousness. hours following use, and the drug has a half-life of 4-24 hours in the body. About 30% of is often self-administered by nasal inhalation, intravenous injection and free-base Amphetamines are excreted in the urine in unchanged form, with the remainder as hydroxylated smoking. It is excreted in the urine in a short time primarily as Benzoylecgonine. 1.2 and deaminated derivatives. Benzoylecgonine, a major metabolite of cocaine, has a longer biological half-life (5-8 hours) than 2 The ECO CUP™ One Step Drug Test yields a positive result when the concentration of cocaine (0.5-1.5 hours), and can generally be detected for 24-48 hours after cocaine exposure. in urine exceeds 1,000 ng/mL. This is the suggested screening cut-off for positive specimens The ECO CUP™ One Step Drug Test yields a positive result when the concentration of Benzoylecgonine set by the Substance Abuse and Mental Health Services Administration (SAMHSA, USA).3 in urine exceeds 300 ng/mL. This is the suggested screening cut-off for positive specimens set by One Step Drug Test the Substance Abuse and Mental Health Services Administration (SAMHSA, USA). 3 Package Insert for Multi Drug Screen Test Cup AMPHETAMINE (AMP 500) COCAINE (COC 150) This Instruction Sheet is for testing of any combination of the following drugs: See AMPHETAMINE (AMP 1000) for the summary. See COCAINE (COC 300) for the summary. AMP/BAR/BZO/BUP/COC/THC/MTD/mAMP/MDMA/MOR/OPI/OXY/PCP/PPX/TCA/EDDP/6-ACM/ETG The ECO CUP™ One Step Drug Test yields a positive result when the concentration of The ECO CUP™ One Step Drug Test yields a positive result when the concentration of Including Adulterant Tests (Specimen Validity Tests) for: Amphetamine in urine exceeds 500 ng/mL. Benzoylecgonine in urine exceeds 150 ng/mL. Oxidants (OX), Specific Gravity (S.G.), pH, Creatinine (CRE), (NIT) and Glutaraldehyde (GLU). A rapid, one step screening test for the simultaneous, qualitative detection of multiple drugs and drug AMPHETAMINE (AMP 300) MARIJUANA (THC 50) metabolites in human urine. See AMPHETAMINE (AMP 1000) for the summary. THC (∆9-) is the primary active ingredient in cannabis (marijuana). When For Forensic Use Only The ECO CUP™ One Step Drug Test yields a positive result when the concentration of smoked or orally administered, THC produces euphoric effects. Users have impaired short term Amphetamine in urine exceeds 300 ng/mL. memory and slowed learning. They may also experience transient episodes of confusion and INTENDED USE anxiety. Long-term, relatively heavy use may be associated with behavioral disorders. The peak (BAR) effect of marijuana administered by smoking occurs in 20-30 minutes and the duration is 90-120 The ECO CUP™ One Step Drug Test is a lateral flow chromatographic immunoassay for the qualitative detection Barbiturates are central nervous system depressants. They are used therapeutically as , minutes after one cigarette. Elevated levels of urinary metabolites are found within hours of of multiple drugs and drug metabolites in urine at the following cut-off concentrations: hypnotics, and . Barbiturates are almost always taken orally as capsules or tablets. exposure and remain detectable for 3-10 days after smoking. The main metabolite excreted in the The effects resemble those of intoxication with . Chronic use of barbiturates leads to Test Calibrator Cut-off urine is 11-nor-∆9-tetrahydrocannabinol-9-carboxylic acid (11-nor-∆9-THC-9-COOH). tolerance and physical dependence. Short acting Barbiturates taken at 400 mg/day for 2-3 months Amphetamine (AMP 1000) D-Amphetamine 1,000 ng/mL The ECO CUP™ One Step Drug Test yields a positive result when the concentration of can produce a clinically significant degree of physical dependence. Withdrawal symptoms 11-nor-Δ9-THC-9-COOH in urine exceeds 50 ng/mL. This is the suggested screening cut-off for positive Amphetamine (AMP 500) D-Amphetamine 500 ng/mL experienced during periods of drug abstinence can be severe enough to cause death. Only a specimens set by the Substance Abuse and Mental Health Services Administration (SAMHSA, Amphetamine (AMP 300) D-Amphetamine 300 ng/mL small amount (less than 5%) of most Barbiturates are excreted unaltered in the urine. USA). 3 Barbiturates (BAR) 300 ng/mL The approximate detection time limits for Barbiturates are: Short acting (e.g. Secobarbital) 100 mg PO (oral) 4.5 days (BZO) 300 ng/mL MARIJUANA (THC 20) Long acting (e.g. ) 400 mg PO (oral) 7 days4 (BUP) Buprenorphine 10 ng/mL See MARIJUANA (THC 50) for the summary. The ECO CUP™ One Step Drug Test yields a positive result when the concentration of Cocaine (COC 300) Benzoylecgonine 300 ng/mL The ECO CUP™ One Step Drug Test yields a positive result when the concentration of Secobarital in urine exceeds 300 ng/mL. 9 Cocaine (COC 150) Benzoylecgonine 150 ng/mL 11-nor-Δ -THC-9-COOH in urine exceeds 20 ng/mL. Marijuana (THC 50) 11-nor-∆9-THC-9-COOH 50 ng/mL BENZODIAZEPINES (BZO) (MTD) 9 Benzodiazepines are medications that are frequently prescribed for the symptomatic treatment of Marijuana (THC 20) 11-nor-∆ -THC-9-COOH 20 ng/mL Methadone is a narcotic analgesic prescribed for the management of moderate to severe pain and anxiety and sleep disorders. They produce their effects via specific receptors involving a Methadone (MTD) Methadone 300 ng/mL for the treatment of opiate dependence (heroin, Vicodin, Percocet, ). The pharmacology neurochemical called gamma aminobutyric acid (GABA). Because they are safer and more of oral Methadone is very different from IV Methadone. Oral Methadone is partially stored in the (mAMP 1000) D-Methamphetamine 1,000 ng/mL effective, Benzodiazepines have replaced barbiturates in the treatment of both anxiety and liver for later use. IV Methadone acts more like heroin. In most states you must go to a pain clinic Methamphetamine (mAMP 500) D-Methamphetamine 500 ng/mL insomnia. Benzodiazepines are also used as sedatives before some surgical and medical or a Methadone maintenance clinic to be prescribed Methadone. Methadone is a long acting pain Methylenedioxymethamphetamine (MDMA) D,L Methylenedioxymethamphetamine 500 ng/mL procedures, and for the treatment of seizure disorders and alcohol withdrawal. reliever producing effects that last from twelve to forty-eight hours. Ideally, Methadone frees the Risk of physical dependence increases if Benzodiazepines are taken regularly (e.g., daily) for Opiate (OPI 300, MOP, MOR) Morphine 300 ng/mL client from the pressures of obtaining illegal heroin, from the dangers of injection, and from the more than a few months, especially at higher than normal doses. Stopping abruptly can bring on Opiate (OPI 2000) Morphine 2,000 ng/mL emotional roller coaster that most opiates produce. Methadone, if taken for long periods and at such symptoms as trouble sleeping, gastrointestinal upset, feeling unwell, loss of appetite, (OXY) Oxycodone 100 ng/mL large doses, can lead to a very long withdrawal period. The withdrawals from Methadone are more sweating, trembling, weakness, anxiety and changes in perception. prolonged and troublesome than those provoked by heroin cessation, yet the substitution and (PCP) Phencyclidine 25 ng/mL Only trace amounts (less than 1%) of most Benzodiazepines are excreted unaltered in the urine; phased removal of methadone is an acceptable method of detoxification for patients and Propoxyphene (PPX) Propoxyphene 300 ng/mL most of the concentration in urine is conjugated drug. The detection period for the therapists.4 Tricyclic (TCA) 1,000 ng/mL Benzodiazepines in the urine is 3-7 days. The ECO CUP™ One Step Drug Test yields a positive result when the concentration of Methadone in urine The yields a positive result when the concentration of 2-Ethylidene-1,5-dimethyl-3,3-dipheylpyrrolidine (EDDP) 2-Ethylidene-1,5-dimethyl-3,3-dipheylpyrrolidine 300 ng/mL ECO CUP™ One Step Drug Test exceeds 300 ng/mL. Oxazepam in urine exceeds 300 ng/mL. 6-Acetylmorphine（ 6-ACM ） 6-Acetylmorphine 10 ng/mL Ethyl Glucuronide (ETG) Ethyl Glucuronide 300 ng/mL METHAMPHETAMINE (mAMP 1000) BUPRENORPHINE (BUP) Methamphetamine is an addictive drug that strongly activates certain systems in the This assay provides only a preliminary qualitative test result. Use a more specific alternate quantitative analytical Buprenorphine is a semisynthetic analgesic derived from thebain, a component of opium. It brain. Methamphetamine is closely related chemically to amphetamine, but the central nervous method to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) is the preferred has a longer duration of action than morphine when indicated for the treatment of moderate to system effects of Methamphetamine are greater. Methamphetamine is made in illegal laboratories confirmatory method.1 Apply clinical and professional judgment to any drug of abuse test result, particularly when severe pain, peri-operative analgesia, and opioid dependence. Low doses buprenorphine and has a high potential for abuse and dependence. The drug can be taken orally, injected, or preliminary positive results are obtained. produces sufficient effect to enable opioid-addicted individuals to discontinue the misuse inhaled. Acute higher doses lead to enhanced stimulation of the central nervous system and of without experiencing withdrawal symptoms. Buprenorphine carries a lower risk of induce euphoria, alertness, reduced appetite, and a sense of increased energy and power. SUMMARY AND EXPLANATION OF THE TEST abuse, addiction, and side effects compared to full opioid because of the “ceiling effect”, Cardiovascular responses to Methamphetamine include increased blood pressure and cardiac which means no longer continue to increase with further increases in dose when reaching a arrhythmias. More acute responses produce anxiety, paranoia, hallucinations, psychotic behavior, The ECO CUP™ One Step Drug Test is a competitive immunoassay utilizing highly specific reactions plateau at moderate doses. However, it has also been shown that Buprenorphine has abuse and eventually, depression and exhaustion. The effects of Methamphetamine generally last 2-4 between antibodies and antigens for the detection of multiple drugs and drug metabolites in human potential and may itself cause dependency. Subutex®, and a Buprenorphine/ hours and the drug has a half-life of 9-24 hours in the body. Methamphetamine is excreted in the urine without the use of an instrument. combination product, Suboxone®, are the only two forms of Buprenorphine that have been urine as amphetamine and oxidized and delaminated derivatives. However, 10-20% of approved by FDA in 2002 for use in opioid addiction treatment. Buprenorphine was rescheduled Methamphetamine is excreted unchanged. Thus, the presence of the parent compound in the AMPHETAMINE (AMP 1000) from Schedule V to Schedule III drug just before FDA approval of Suboxone and Subutex. urine indicates Methamphetamine use. Methamphetamine is generally detectable in the urine for PN: Amphetamine is a Schedule II controlled substance available by prescription (Dexedrine®) and is The ECO CUP™ One Step Drug Test yields a positive result when the concentration of 3-5 days, depending on urine pH level.

Y03 11 109905 also available on the illicit market. Amphetamines are a class of potent sympathomimetic agents Buprenorphine in urine exceeds 10 ng/mL. The ECO CUP™ One Step Drug Test yields a positive result when the concentration of Methamphetamine in with therapeutic applications. They are chemically related to the human body's natural urine exceeds 1,000 ng/mL. : epinephrine and . Acute higher doses lead to enhanced COCAINE (COC 300) stimulation of the central nervous system and induce euphoria, alertness, reduced appetite, and a Cocaine is a potent central nervous system (CNS) stimulant and a local anesthetic. Initially, it METHAMPHETAMINE (mAMP 500) sense of increased energy and power. Cardiovascular responses to Amphetamines include brings about extreme energy and restlessness while gradually resulting in tremors, over-sensitivity See METHAMPHETAMINE (mAMP 1000) for the summary. increased blood pressure and cardiac arrhythmias. More acute responses produce anxiety, and spasms. In large amounts, cocaine causes fever, unresponsiveness, difficulty in breathing and The ECO CUP™ One Step Drug Test yields a positive result when the concentration of Methamphetamine in urine exceeds 500 ng/mL. The ECO CUP™ One Step Drug Test yields a positive result when the concentration of Phencyclidine in the blood, hair and urine. By using, the ECO CUP™ One Step Drug Test EtG can be detect in urine exceeds 25 ng/mL. This is the suggested screening cut-off for positive specimens set by the urine, confirming the consumption of alcohol. The EtG metabolite remains in the body longer and METHYLENEDIOXYMETHAMPHETAMINE (MDMA) Substance Abuse and Mental Health Services Administration (SAMHSA, USA).3 therefore has a more useful window of detection of 8 to 80 hours. EtG testing is an excellent Methylenedioxymethamphetamine (ecstasy) is a designer drug first synthesized in 1914 by a option for zero-tolerance alcohol consumption or for rehabilitation programs. 8 German drug company for the treatment of obesity. Those who take the drug frequently report PROPOXYPHENE (PPX) The ECO CUP™ One Step Drug Test yields a positive result when the concentration of Ethyl adverse effects, such as increased muscle tension and sweating. MDMA is not clearly a stimulant, Propoxyphene is a mild narcotic analgesic found in various pharmaceutical preparations, usually Glucuronide in urine exceeds 300 ng/mL although it has, in common with amphetamine drugs, a capacity to increase blood pressure and as the hydrochloride or napsylate salt. These preparations typically also contain large amounts heart rate. MDMA does produce some perceptual changes in the form of increased sensitivity to of acetaminophen, , or . Peak plasma concentrations of propoxyphene are achieved ADULTERANT TESTS (SPECIMEN VALIDITY TESTS) SUMMARY light, difficulty in focusing, and blurred vision in some users. Its mechanism of action is thought to from 1 to 2 hours post dose. In the case of overdose, propoxyphene blood concentrations be via release of the serotonin. MDMA may also release , although the can reach significantly higher levels. In human, propoxyphene is metabolized by N-demethylation The adulterant test strip contains chemically treated reagent pads. Observation of the color general opinion is that this is a secondary effect of the drug (Nichols and Oberlender, 1990). The to yield . Norpropoxyphene has a longer half-life (30 to 36 hours) than parent change on the strip compared to the color chart provides a semi-quantitative screen for oxidants, most pervasive effect of MDMA, occurring in virtually all people who took a reasonable dose of the propoxyphene (6 to 12 hours). The accumulation of norpropoxyphene seen with repeated doses specific gravity, pH, creatinine, nitrite and glutaraldehyde in human urine which can help to assess drug, was to produce a clenching of the jaws. may be largely responsible for resultant toxicity. the integrity of the urine specimen. The ECO CUP™ One Step Drug Test yields a positive result when the concentration of The ECO CUP™ One Step Drug Test yields a positive result when the concentration of Methylenedioxymethamphetamine in urine exceeds 500 ng/mL. Propoxyphene or Norpropoxyphene in urine exceeds 300 ng/mL. ADULTERATION OPIATE (OPI 300, MOR, MOP) TRICYCLIC ANTIDEPRESSANTS (TCA) Opiate refers to any drug that is derived from the opium poppy, including the natural products, Tricyclic Antidepressants (TCA) are commonly used for the treatment of depressive disorders. Adulteration is the tampering of a urine specimen with the intention of altering the test results. The morphine and , and the semisynthetic drugs such as heroin. Opioid is more general, TCA overdoses can result in profound central nervous system depression, cardiotoxicity and use of adulterants in the urine specimen can cause false negative results by either interfering with referring to any drug that acts on the opioid receptor. anticholinergic effects. TCA overdose is the most common cause of death from prescription drugs. the test and/or destroying the drugs present in the urine. Dilution may also be used to produce Opioid analgesics comprise a large group of substances which control pain by depressing the TCAs are taken orally or sometimes by injection. TCAs are metabolized in the liver. Both TCAs false negative drug test results. To determine certain urinary characteristics such as specific central nervous system. Large doses of morphine can produce higher tolerance levels, and their metabolites are excreted in urine mostly in the form of metabolites for up to ten days. gravity and pH, and to detect the presence of oxidants, nitrite, glutaraldehyde and creatinine in physiological dependency in users, and may lead to substance abuse. Morphine is excreted The ECO CUP™ One Step Drug Test yields a positive result when the concentration of Nortriptyline urine are considered to be the best ways to test for adulteration or dilution. unmetabolized, and is also the major metabolic product of codeine and heroin. Morphine is in urine exceeds 1,000 ng/mL. • Oxidants (OX): Tests for the presence of oxidizing agents such as bleach and peroxide in the 4 detectable in the urine for several days after an opiate dose. urine. The ECO CUP™ One Step Drug Test yields a positive result when the concentration of Morphine 2-ETHYLIDENE-1,5-DIMETHYL-3,3-DIPHEYLPYRROLIDINE (EDDP) • Specific Gravity (S.G.): Tests for sample dilution. Normal levels for specific gravity will range in urine exceeds 300 ng/mL. EDDP is an immunoassay based on the principle of competitive binding. Drugs which may be from 1.003 to 1.030. Specific gravity levels of less than 1.003 or higher than 1.030 may be an present in the urine specimen compete against the drug conjugate for binding sites on the indication of adulteration or specimen dilution. OPIATE (OPI 2000) antibody. • pH: Tests for the presence of acidic or alkaline adulterants in urine. Normal pH levels should be Opiate refers to any drug that is derived from the opium poppy, including the natural products, During testing, a urine specimen migrates upward by capillary action. EDDP, if present in the urine in the range of 4.0 to 9.0. Values below pH 4.0 or above pH 9.0 may indicate the sample has morphine and codeine, and the semisynthetic drugs such as heroin. Opioid is more general, specimen below 300 ng/mL, will not saturate the binding sites of antibody coated particles in the been altered. referring to any drug that acts on the opioid receptor. test device. The antibody-coated particles will then be captured by immobilized EDDP conjugate • Nitrite (NIT): Tests for commercial adulterants such as Klear and Whizzies. Normal urine Opioid analgesics comprise a large group of substances which control pain by depressing the and a visible colored line will show up in the test line region. The colored line will not form in the specimens should contain no trace of nitrite. Positive results for nitrite usually indicate the central nervous system. Large doses of morphine can produce higher tolerance levels, test line region if the EDDP level exceeds 300 ng/mL because it will saturate all the binding sites presence of an adulterant. physiological dependency in users, and may lead to substance abuse. Morphine is excreted of anti-EDDP antibodies. A drug-positive urine specimen will not generate a colored line in the test • Glutaraldehyde (GLU): Tests for the presence of an aldehyde. Glutaraldehyde is not normally unmetabolized, and is also the major metabolic product of codeine and heroin. Morphine is line region, while a drug-negative urine specimen or a specimen containing a drug concentration found in a urine specimen. Detection of glutaraldehyde in a specimen is generally an indicator detectable in the urine for several days after an opiate dose.4 less than the cut-off will generate a line in the test line region. To serve as a procedural control, a of adulteration. The ECO CUP™ One Step Drug Test yields a positive result when the concentration of Morphine colored line will always appear at the control line region indicating that proper volume of specimen • Creatinine (CRE): Creatinine is one way to check for dilution and flushing, which are the most in urine exceeds 2,000 ng/mL. This is the suggested screening cut-off for positive specimens set has been added and membrane wicking has occurred. common mechanisms used in an attempt to circumvent drug testing. Low creatinine may by the Substance Abuse and Mental Health Services Administration (SAMHSA, USA).3 The ECO CUP™ One Step Drug Test yields a positive result when the concentration of indicate dilute urine. 2-Ethylidene-1,5-dimethyl-3,3-dipheylpyrrolidine in urine exceeds 300ng/mL. OXYCODONE (OXY) O x ycodone,[4 , 5-epoxy-14-hy droxy-3-met hox y-17-met h y l-morphinan-6-one, 6-ACETYLMORPHINE (6-ACM) PRINCIPLE dihydrohydroxycodeinone] is a semisynthetic opioid agonist derived from thebaine, a constituent 6-Acetylmorphine（6-ACM）is one of three active metabolites of heroin (diacetylmorphine), the of opium. Oxycodone is a Schedule II narcotic analgesic and is widely used in clinical medicine. others being morphine and the much less active 3-acetylmorphine (3-ACM). 6-ACM is rapidly The ECO CUP™ One Step Drug Test is an immunoassay based on the principle of competitive The pharmacology of oxycodone is similar to that of morphine, in all respects, including its abuse created from heroin in the body, and then is either metabolized into morphine or excreted in the binding. Drugs which may be present in the urine specimen compete against their respective drug and dependence liabilities. Pharmacological effects include analgesia, euphoria, feelings of urine. Since 6-ACM is a unique metabolite to heroin, its presence in the urine confirms that heroin conjugate for binding sites on their specific antibody. relaxation, respiratory depression, constipation, papillary constriction, and cough suppression. was the opioid used. This is significant because on a urine immunoassay drug screen, the test Oxycodone is prescribed for the relief of moderate to high pain under pharmaceutical trade names typically tests for morphine, which is a metabolite of a number of legal and illegal opiates/opioids During testing, a urine specimen migrates upward by capillary action. A drug, if present in the urine as OxyContin® (controlled release), OxyIR®, OxyFast®(immediate release formulations), or such as codeine, morphine sulphate, and heroin. 6-ACM remains in the urine for no more than 24 specimen below its cut-off concentration, will not saturate the binding sites of its specific antibody. Percodan® (aspirin) and Percocet® (acetaminophen) that are in combination with other hours so a urine specimen must be collected soon after the last heroin use, but the presence of The antibody will then react with the drug-protein conjugate and a visible colored line will show up nonnarcotic analgesics. Oxycodone's behavioral effects can last up to 5 hours. The 6-ACM guarantees that heroin was in fact used as recently as within the last day. in the test line region of the specific drug strip. The presence of drug above the cut-off controlled-release product, OxyContin®, has a longer duration of action (8-12 hours). The ECO CUP™ One Step Drug Test yields a positive result when the concentration of 6-Acetylmorphin e concentration will saturate all the binding sites of the antibody. Therefore, the colored line will not The ECO CUP™ One Step Drug Test yields a positive result when the concentration of Oxycodone in urine exceeds 10 ng/mL. form in the test line region. in urine exceeds 100 ng/mL. ETHYL GLUCURONIDE (ETG) A drug-positive urine specimen will not generate a colored line in the specific test line region of the strip because of drug competition, while a drug-negative urine specimen will generate a line in the PHENCYCLIDINE (PCP) Ethyl Glucuronide (EtG) is a direct metabolite of ethanol, which is formed by enzymatic 10, 11 test line region because of the absence of drug competition. Phencyclidine, also known as PCP or Angel Dust, is a hallucinogen that was first marketed as a conjugation of ethanol with glucuronic acid. Alcohol in urine is normally detected for only a few hours, whereas EtG can be detected up to several days even after complete elimination of alcohol surgical anesthetic in the 1950's. It was removed from the market because patients receiving it To serve as a procedural control, a colored line will always appear at the control line region, from the body.12 Therefore, EtG can be a useful diagnostic biomarker for determining recent became delirious and experienced hallucinations. indicating that proper volume of specimen has been added and membrane wicking has occurred. alcohol use and in monitoring abstinence in alcoholics in alcohol withdrawal treatment Phencyclidine is used in powder, capsule, and tablet form. The powder is either snorted or smoked programs.13-16 Ethanol can be produced in vitro due to fermentation of urine samples containing after mixing it with marijuana or vegetable matter. Phencyclidine is most commonly administered 17 sugars, bacteria or yeast when samples are exposed to warm temperatures. In such cases, EtG by inhalation but can be used intravenously, intra-nasally, and orally. After low doses, the user test can be used, as a confirmatory test to determine if the alcohol in the sample is due to REAGENTS thinks and acts swiftly and experiences mood swings from euphoria to depression. Self-injurious consumption of alcohol or it is formed in vitro as a result of fermentation. Currently EtG is monitor behavior is one of the devastating effects of Phencyclidine. by GC/MS and LC/MS/MS.18-19 The test contains a membrane strip coated with drug-protein conjugates (purified bovine albumin) PCP can be found in urine within 4 to 6 hours after use and will remain in urine for 7 to 14 days, Ethyl glucuronide (EtG) is a minor non-oxidative metabolite of ethyl alcohol formed by the in vivo on the test line, a goat polyclonal antibody against gold-protein conjugate at the control line, and a depending on factors such as metabolic rate, user's age, weight, activity, and diet.5 Phencyclidine conjugation of ethanol with glucuronic acid with UDP glucuronosyltransferase. EtG is a product of dye pad which contains colloidal gold particles coated with mouse monoclonal antibody specific to is excreted in the urine as an unchanged drug (4% to 19%) and conjugated metabolites (25% to 30%).6 metabolic process of ingested alcohol (ethanol) rapidly metabolized in the body, which is excrete in individual drug on the list in the Intended Use section. ADULTERANT TESTS (SPECIMEN VALIDITY TESTS) REAGENTS LIMITATIONS

Adulteration Pad Reactive Indicator Buffers and Non-reactive Ingredients 1. The ECO CUP™ One Step Drug Test provides only a qualitative, preliminary analytical result. Oxidants (OX) 0.36% 99.64% A secondary analytical method must be used to obtain a confirmed result. Gas Specific Gravity (S.G.) 0.25% 99.75% chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method. 3,4,7 pH 0.06% 99.94% 2. There is a possibility that technical or procedural errors, as well as other interfering substances Nitrite（NIT） 0.07% 99.93% 5 in the urine specimen may cause erroneous results. Glutaraldehyde（GLU） 0.02% 99.98% 3. Adulterants, such as bleach and/or alum, in urine specimens may produce erroneous results Creatinine（CRE） 0.04% 99.96% regardless of the analytical method used. If adulteration is suspected, the test should be (Fig. 1) (Fig. 2) (Fig. 3) repeated with another urine specimen and a new test cup. PRECAUTIONS 4. A positive result does not indicate intoxication of the donor, the concentration of drug in the urine, or the route of drug administration. • For Forensic Use Only. 5. A negative result may not necessarily indicate drug-free urine. Negative results can be obtained • Do not use after the expiration date. when drug is present but below the cut-off level of the test. • The test cup should remain in the sealed pouch until use. 6. Test does not distinguish between drugs of abuse and certain medications. • While urine is not classified by OSHA or the CDC as a biological hazard unless visibly C C C 7. A positive test result may be obtained from certain foods or food supplements. contaminated with blood8,9 , the use of gloves is recommended to avoid unnecessary contact T T T with the specimen. NEGATIVE POSITIVE INVALID PERFORMANCE CHARACTERISTICS • The used test cup and urine specimen should be discarded according to federal, state and local (Fig. 4) regulations. • The test is for single use. Accuracy INTERPRETATION OF RESULTS Testing on accuracy of the test strips was performed on clinical specimens collected for each of the following drug types. All clinical specimens were quantified by GC/MS analysis before testing. STORAGE AND STABILITY The quantity of the following compounds were analyzed by GC/MS and contributed to the total (Please refer to the previous illustration) amount of drugs found in the positive specimens tested. NEGATIVE: Two lines appear.* One color line should be in the control region (C), and another Store as packaged in the sealed pouch at 2-30oC (36-86oF). The test is stable through the apparent color line adjacent should be in the test region (T). This negative result indicates that the expiration date printed on the sealed pouch. The test cup must remain in the sealed pouch until drug concentration is below the detectable level. Test Compounds Contributed to the Totals of GC/MS use. DO NOT FREEZE. Do not use beyond the expiration date. *NOTE: The shade of color in the test line region (T) will vary, but it should be considered negative AMP Amphetamine whenever there is even a faint distinguishable color line. BAR Secobarbital BZO Oxazepam SPECIMEN COLLECTION AND PREPARATION POSITIVE: One color line appears in the control region (C). No line appears in the test region (T). This positive result indicates that the drug concentration is above the detectable level. BUP Buprenorphine Urine Assay COC Benzoylecgonine INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural 9 The urine specimen should be collected directly into the test cup. Urine collected at any time of the THC 11-nor-∆ -tetrahydrocannabinol-9-carboxylic acid techniques are the most likely reasons for control line failure. Review the procedure and repeat day may be used. If the urine specimen is collected for later testing, another dry and clean MTD Methadone the test using a new test cup. If the problem persists, discontinue using the lot immediately and container should be used to collect the specimen. mAMP Methamphetamine contact your supplier. MDMA D,L Methylenedioxymethamphetamine, Methylenedioxyamphetamine Morphine, Codeine ADULTERANT TESTS (SPECIMEN VALIDITY TESTS) INTERPRETATION OPI, MOR SPECIMEN STORAGE OXY Oxycodone (Please refer to the color chart) PCP Phencyclidine o o Urine specimen collected for later testing may be stored at 2-8 C（36-46 F）for up to 48 hours. Semi-quantitative results are obtained by visually comparing the reacted color blocks on the strip PPX Propoxyphene o For prolonged storage, specimens may be frozen and stored below -20 C. Frozen specimens to the printed color indicator on the color chart. No instrumentation is required. TCA Nortriptyline should be thawed and mixed well before testing. EDDP 2-Ethylidene-1,5-dimethyl-3,3-dipheylpyrrolidine ADULTERANT TESTS (SPECIMEN VALIDITY TESTS) LIMITATIONS 6-ACM 6-Acetylmorphine ETG Ethyl Glucuronide MATERIALS 1. The adulterant tests included with the product are meant to aid in the determination of abnormal specimens, but may not cover all the possible adulterants. Materials Provided 2. Oxidants: Normal human urine should not contain oxidants. The presence of high level of The following results are tabulated from these clinical studies: • Test cup • Package insert • Procedure card • Security seal label antioxidants in the specimen, such as ascorbic acid, may result in false negative results for the • Color chart card for adulterant interpretation (when applicable) oxidants pad. % Agreement with GC/MS (HPLC for TCA) Materials Required But Not Provided 3. Specific Gravity: Elevated levels of protein in urine may cause abnormally high specific gravity values. • Timer • Disposable gloves AMP mAMP OPI 2000 OPI 300 COC PCP AMP300 CO C150 THC20 mAMP500 AMP500 4. Nitrite: Nitrite is not a normal component of human urine. However, nitrite found in urine may Positive indicate urinary tract infections or bacterial infections. Nitrite levels of > 20 mg/dL may produce Agreement 95% 96% >99% 96% 96% 95% >99% >99% >99% >99% >99% DIRECTIONS FOR USE false positive glutaraldehyde results. Negative Agreement >99% >99% 97% >99% >99% >99% 98% >99% >99% >99% 95% 5. Glutaraldehyde: Is not normally found in a urine specimen. However certain metabolic o o Overall Allow the test cup to come to room temperature [15-30 C (59-86 F)] prior to testing. abnormalities such as ketoacidosis (fasting, uncontrolled diabetes or high-protein diets) may Agreement 98% 98% 98% 98% 98% 95% 99% >99% >99% >99% 98% 1) Tear the foil pouch open, remove test cup, and donor needs to obtain their own disposable gloves. interfere with the test results. 6. Creatinine: Tests for the specimen for dilution and flushing. Normal creatinine levels are Label the test cup with donor information. (Fig. 1) between 20 and 350 mg/dL. Under rare conditions, certain kidney diseases may show dilute BAR TCA MDMA BZO MTD OXY EDDP THC PPX BUP 6-ACM ETG 2) Wear disposable gloves to collect urine specimen. Open test cup lid. Urinate directly into the urine. PN: Positive test cup. Be sure to fill up the test cup with the urine specimen between minimum 30mL to Agreement 97% 98% 93% 96% 94% 95% 98% 96% 95% 93% 98% >99%

Y03 11 109905 maximum 70mL (marked on the cup). (Fig. 2) QUALITY CONTROL Negative 98% >99% >99% >99% 98% >99% 95% 98% 95% >99% >99% 3) After urine specimen has been collected, close the lid securely and return cup to collection Agreement >99% Overall official. (Fig. 3) A procedural control is included in the test. A color line appearing in the control region (C) is Agreement 98% 99% 96% 98% 96% 98% 96% 98% 96% 94% 99% >99% 4) The collection official will use newly obtained gloves to peel off the label to reveal test results. Read considered an internal procedural control. It confirms sufficient specimen volume, adequate test results at 5 minutes. DO NOT INTERPRET RESULTS AFTER 10 MINUTES. (Fig. 4) membrane wicking and correct procedural technique.

THC20 AMP300 AMP500 BENZODIAZEPINES (BZO) METHAMPHETAMINE (mAMP 1000) Methamphetamine 0 40 0 42 0 20 Oxazepam Total number of Result Precision Total number of Result Precision conc.(ng/mL) Determinations conc.(ng/mL) Determinations 0 3 1 6 2 18 No drug present 40 40 negative >99% No drug present 40 40 negative >99% 3 0 3 0 20 0 150 40 40 negative >99% 500 40 40 negative >99% 750 40 40 negative >99% 47 0 40 0 20 0 225 40 40 negative >99% 1,000 40 40 positive >99% >99% >99% >99% 98% >99% 95% 300 40 40 positive >99% 450 40 40 positive >99% 1,500 40 40 positive >99%

PPX EDDP BUP COC150 mAMP500 6-ACM ETG Pos Neg COCAINE (COC 300) 0 20 0 20 0 20 0 40 0 42 0 20 0 70 METHAMPHETAMINE (mAMP 500)

1 19 2 18 2 18 0 6 0 6 0 20 0 70 Benzoylecgonine Total number of Result Precision Methamphetamine Total number of Result Precision conc.(ng/mL) Determinations conc.(ng/mL) Determinations 18 2 19 1 17 3 4 0 11 0 19 1 70 0 No drug present 40 40 negative >99% No drug present 60 60 negative >99% 20 0 20 0 20 0 51 0 31 0 20 0 70 0 150 40 40 negative >99% 250 30 30 negative >99% 95% 98% 98% 95% 93% 95% >99% >99% >99% >99% 98% >99% >99% >99% 225 40 40 negative >99% 375 15 15 negative >99% 375 40 40 positive >99% 625 15 12 positive >80% Reproducibility 450 40 40 positive >99% 750 30 30 positive >99% 1000 30 30 positive >99% Reproducibility studies were carried out using diluted solutions of the commercially available stock COCAINE (COC 150) solutions of the drug analytes listed. Dilutions were made from the stock solution of each drug to METHYLENEDIOXYMETHAMPHETAMINE (MDMA) the concentrations specified in the following tables. The results are listed in the following tables. Benzoylecgonine Total number of Result Precision conc.(ng/mL) Determinations Methylenedioxymeth- Total number of Result Precision amphetamine AMPHETAMINE (AMP 1000) No drug present 60 60 negative >99% conc.(ng/mL) Determinations Amphetamine Total number of Result Precision 75 30 30 negative >99% No drug present 40 40 negative >99% conc.(ng/mL) Determinations 112.5 15 15 negative >99% 250 40 40 negative >99% No drug present 40 40 negative >99% 187.5 15 11 positive >73% 375 40 40 negative >99% 500 40 40 negative >99% 225 30 29 positive >96% 500 40 40 positive >99% 750 40 40 negative >99% 300 30 30 positive >99% 750 40 40 positive >99% 1,000 40 40 positive >99% 40 40 positive >99% 1,500 MARIJUANA (THC 50) OPIATE (OPI 300, MOP, MOR) 11-nor-∆9-THC-9-COOH Total number of Result Precision conc.(ng/mL) Determinations Morphine Total number of Result Precision AMPHETAMINE (AMP 500) conc.(ng/mL) Determinations No drug present 40 40 negative >99% Amphetamine Total number of Result Precision No drug present 40 40 negative >99% conc.(ng/mL) Determinations 25 40 40 negative >99% 150 40 40 negative >99% N o drug present 40 40 negative >99% 37.5 40 40 negative >99% 225 40 40 negative >99% 250 40 40 negative >99% 50 40 40 positive >99% 300 40 40 positive >99% 750 40 40 positive >99% 75 40 40 positive >99% 375 40 40 positive >99% 1000 40 40 positive >99%

MARIJUANA (THC 20) OPIATE (OPI 2000) AMPHETAMINE (AMP 300) 11-nor-∆9-THC-9-COOH Total number of Result Precision conc.(ng/mL) Determinations Morphine Total number of Result Precision Amphetamine Total number of Result Precision conc.(ng/mL) Determinations conc.(ng/mL) Determinations No drug present 60 60 negative >99% No drug present 40 40 negative >99% No drug present 60 60 negative >99% 10 30 29 negative 97% 1,000 40 40 negative >99% 150 30 30 negative >99% 15 15 9 negative 60% 1,500 40 40 negative >99% 225 15 15 negative >99% 25 15 12 positive >80% 2,000 40 40 positive >99% 375 15 15 positive >99% 30 30 29 positive 97% 3,000 40 40 positive >99% 450 30 30 positive >99% 40 30 30 positive >99% 600 30 30 positive >99%

BARBITURATES (BAR) METHADONE (MTD) OXYCODONE (OXY) Secobarbital Total number of Result Precision Methadone Total number of Result Precision Oxycodone Total number of Result Precision conc.(ng/mL) Determinations conc.(ng/mL) Determinations conc.(ng/mL) Determinations No drug present 40 40 negative >99% No drug present 40 40 negative >99% No drug present 40 40 negative >99% 150 40 40 negative >99% 150 40 40 negative >99% 50 40 40 negative >99% 225 40 40 negative >99% 225 40 40 negative >99% 75 40 40 negative >99% 300 40 40 positive >99% 300 40 40 positive >99% 100 40 40 positive >99% 450 40 40 positive >99% 450 40 40 positive >99% 150 40 40 positive >99% PHENCYCLIDINE (PCP) Analytical Sensitivity (+/-)-Methylenedioxyamphetamine 1,500 Phencyclidine Total number of Result Precision A drug-free urine pool was spiked with drugs at concentrations listed. The results are summarized conc.(ng/mL) Determinations below. Amphetamine (AMP 500) No drug present 40 40 negative >99% d-amphetamine 500 12.5 40 40 negative >99% D,l-amphetamine 750 19 40 40 negative >99% Drug concentration n AMP 1000 BAR BZO COC 300 THC 50 l-amphetamine 16,000 Cut-off Range 25 40 40 positive >99% - + - + - + - + - + 650 37.5 40 40 positive >99% 0% Cut-off 10 10 0 10 0 10 0 10 0 10 0 (+/-)-Methylenedioxyamphetamine 800 -50% Cut-off 10 10 0 10 0 10 0 10 0 10 0 Amphetamine (AMP 300) TRICYCLIC ANTIDEPRESSANTS (TCA) -25% Cut-off 10 10 0 10 0 10 0 10 0 10 0 d-amphetamine 300 Total number of Result Precision Cut-off 10 0 10 0 10 0 10 0 10 0 10 Nortriptyline d,l-amphetamine 500 conc.(ng/mL) Determinations +25% Cut-off 10 0 10 0 10 0 10 0 10 0 10 l-amphetamine 10,000 No drug present 40 40 negative >99% +50% Cut-off 10 0 10 0 10 0 10 0 10 0 10 Phentermine 400 500 40 40 negative >99% (+/-)- Methylenedioxyamphetamine 500 750 40 40 negative >99% 1,000 40 40 positive >99% Barbiturates (BAR) 1,500 40 40 positive >99% Drug concentration n MTD mAMP1000 MDMA MOP OPI 2000 OXY PCP TCA Secobarbital 300 Cut-off Range - + - + - + - + - + - + - + - + 300 BUPRENORPHINE (BUP) 0% Cut-off 10 10 0 10 0 10 0 10 0 10 0 10 0 10 0 10 0 Alphenol 150 Buprenorphine Total number of Result Precision -50% Cut-off 10 10 0 10 0 10 0 10 0 10 0 10 0 10 0 10 0 200 conc.(ng/mL) Determinations 75 -25% Cut-off 10 10 0 10 0 10 0 10 0 10 0 10 0 10 0 10 0 2,500 No drug present 60 60 negative >99% Cut-off 10 0 10 0 10 0 10 0 10 0 10 0 10 0 10 0 10 5 60 60 negative >99% Butethal 100 +25% Cut-off 10 0 10 0 10 0 10 0 10 0 10 0 10 0 10 0 10 15 60 60 positive >99% Cyclopentobarbital 600 0 10 0 10 0 10 +50% Cut-off 10 0 10 0 10 0 10 0 10 0 10 300 PROPOXYPHENE (PPX) Phenobarbital 100 Propoxyphene Total number of Result Precision conc.(ng/mL) Determinations Benzodiazepines (BZO) Drug concentration n AMP 300 COC 150 THC 20 mAMP 500 Oxazepam 300 No drug present 60 60 negative >99% Cut-off Range - + - + - + - + a-Hydroxyalprazolam 1,260 150 60 60 negative >99% 0% Cut-off 25 25 0 25 0 25 0 25 0 200 450 60 60 positive >99% -50% Cut-off 25 25 0 25 0 25 0 25 0 1,560 -25% Cut-off 25 25 0 25 0 25 0 25 0 1,565 2-ETHYLIDENE-1,5-DIMETHYL-3,3-DIPHEYLPYRROLIDINE (EDDP) Cut-off 25 1 24 3 22 2 23 2 23 Chlordiazepoxide HCl 780 EDDP Total number of Result Precision 100 +25% Cut-off 25 0 25 0 25 0 25 0 25 conc.(ng/mL) Determinations 785 +50% Cut-off 25 0 25 0 25 0 25 0 25 No drug present 60 60 negative >99% Dipotassium 195 150 60 60 negative >99% 1,560 450 60 60 positive >99% Desalkylflurazepam 390 Drug concentration EDDP BUP PPX 6-ACM AMP500 ETG n n 195 Cut-off Range - + - + - + - + - + - + 2,500 6-ACETYLMORPHINE（6-ACM） 0% Cut-off 90 90 0 90 0 90 0 90 0 90 0 30 30 0 385 Total number of -50% Cut-off 90 90 0 90 0 90 0 90 0 90 0 30 30 0 6-Acetylmorphine Result Precision (±) 1,560 conc.(ng/mL) Determinations -25% Cut-off 90 72 18 80 10 75 15 80 10 81 9 30 30 0 RS-Lorazepam glucuronide 160 N o drug present 40 40 negative >99% Cut-off 90 47 43 46 44 50 40 46 44 45 45 30 3 27 12,500 5 40 40 negative >99% +25% Cut-off 90 9 81 16 74 12 78 12 78 10 80 30 1 29 +50% Cut-off 0 90 0 90 0 90 95 15 40 40 positive >99% 90 0 90 0 90 30 0 30 2X Cut-off 90 0 90 0 90 0 90 0 90 0 90 30 0 30 Norchlordiazepoxide 200 20 40 40 positive >99% Nordiazepam 390 Analytical Specificity 100 ETHYL GLUCURONIDE (ETG) 2,500 The following table lists the concentration of compounds (ng/mL) that were detected positive in urine by ECO CUP™ One Step Drug Test at a read time of 5 minutes. Ethyl Glucuronide Total number of Result Precision Buprenorphine (BUP) conc. (ng/mL) Determinations Drug Concentration (ng/mL) Buprenorphine 10 PN: Negative 70 70 negative >99% Amphetamine (AMP 1000) Norbuprenorphine 20 Y03 11 109905 150 70 70 negative >99% d-amphetamine 1,000 D,l-amphetamine 1,000 Cocaine (COC 300) 450 70 70 positive >99% l-amphetamine 20,000 Benzoylecgonine 300 600 70 70 positive >99% Phentermine 1,250 Cocaethylene 300 Cocaine 300 Morphine-3-glucuronide 75 Codeine 10 80,000 Oxycodone 75,000 Diacetylmorphine 50 75,000 Thebaine 13,000 Ethylmorphine 24 Hydrocodone 100 Cocaine (COC 150) OPIATE (OPI 2000) Hydromorphone 100 Benzoylecgonine 150 6-acetylmorphine 1,000 Levorphanol 400 Cocaethylene 2,500 Codeine 800 Morphine3-B-D-Glucuronide 50 Cocaine 1,000 Ethylmorphine 400 Nalorphine 10,000 Heroin 10,000 Normorphine 12,500 Marijuana (THC 50) Hydromorphone 2,000 Norcodeine 15,000 11-Nor-∆9-THC-9-COOH 50 Hydrocodone 5,000 Oxycodone 25,000 11-Hydroxy-Δ9-Tetrahydrocannabinol 5,000 Morphine 2,000 Oxymorphone 25,000 11-Nor-Δ8-THC-9-COOH 50 Morphine-3-glucuronide 1,000 Thebaine 1,500 11-Nor-Δ9-Tetrahydrocannabinol-9 Carboxylic Glucuronide 2,500 Oxycodone 50,000 Δ8-THC 20,000 Thebaine 26,000 Ethyl Glucuronide (EtG) Δ9-THC 20,000 Ethyl-β-D-glucuronide 300 Oxycodone (OXY) Marijuana (THC 20) Oxycodone 100 EFFECT OF URINARY SPECIFIC GRAVITY 11-nor-∆9-THC -9-COOH 20 Codeine 50,000 11-nor-∆8-THC -9-COOH 50 Dihydrocodeine 12,500 Fifteen (15) urine samples of normal, high, and low specific gravity ranges (1.005, 1.015, 1.03) Cannabinol 15,000 Ethylmorphine 25,000 were spiked with drugs at 50% below and 50% above cut-off levels respectively. The ECO CUP™ ∆9-THC 10,000 Hydrocodone 1,580 One Step Drug Test was tested in duplicate using ten drug-free urine and spiked urine samples. 8 The results demonstrate that varying ranges of urinary specific gravity do not affect the test ∆ -THC 10,000 Hydromorphone 12,500 results. Oxymorphone 1,580 Methadone （MTD） Thebaine 50,000 EFFECT OF THE URINARY PH Methadone 300 50,000 Phencyclidine (PCP) The pH of an aliquoted negative urine pool was adjusted to pH ranges of 4.0, 4.5, 5.0, 6.0 and 9.0, Phencyclidine 25 and spiked with drugs at 50% below and 50% above cut-off levels. The spiked, pH-adjusted urine Methamphetamine (mAMP) 4-Hydroxy PCP 90 was tested with the ECO CUP™ One Step Drug Test. The results demonstrate that varying (+/-) 3,4-Methylenedioxy-n-ethylamphetamine 20,000 PCP Morpholine 625 ranges of pH do not interfere with the performance of the test. Procaine (Novocaine) 60,000 20,000 Propoxyphene (PPX) INTERFERENCE +/-methamphetamine 1,000 D-Propoxyphene 300 +methamphetamine 500 D-Norpropoxyphene 300 A study was conducted to determine the cross-reactivity of the test with compounds in either Ranitidine (Zantac) 50,000 drug-free urine or drug positive urine containing Amphetamine, Barbiturates, Benzodiazepines, Methylenedioxymethamphetamine 2,500 Tricyclic Antidepressants (TCA) Buprenorphine, Cocaine, Marijuana, Methadone, Methamphetamine, MDMA (Ecstasy), Opiate, Nortriptyline 1,000 Morphine, Oxycodone, Phencyclidine, Propoxyphene, Tricyclic Antidepressants, EDDP(Methadone Methamphetamine (mAMP 500) 1,500 metabolite), 6-Acetylmorphine or Ethyl Glucuronide . The following compounds show no cross-reactivity d-Methamphetamine 500 12,500 when tested with the ECO CUP™ One Step Drug Test at concentrations of 100ug/mL. d,l-Methamphetamine 1,000 200 Cocaine, Benzodiazepines, Amphetamine, Methamphetamine, Marijuana, Opiate, Ranitidine 500,000 2,000 Morphine Oxycodone, Phencyclidine, Barbiturates, Buprenorphine, Propoxyphene, Procaine 200,000 400 EDDP, 6-Acetylmorphine.， Non Cross-Reacting Compounds Methylenedioxyamphetamine 90,000 2,000 *Parent compound only： Methylenedioxymethamphetamine 1,000 2,500 Nordoxepin DL-Aminoglutethimide Methylenedioxyethylamphetamine 10,000 1,500 Acetaldehyde 2,500 Acetaminophen Amitryptyline Methylenedioxymethamphetamine (MDMA) 3,000 Acetamidophenol( N-Acetyl-p-aminophenol ) Ammonium Chloride Acetazolamide Methylenedioxymethamphetamine 500 Amphotericin B 3,4-Methylenedioxyamphetamine 3,000 2-Ethylidene-1,5-dimethyl-3,3-dipheylpyrrolidine (EDDP) Acetophenetidin Ampicillin (+/-)-3,4-Methylenedioxy-n-ethylamphetamine 300 EDDP 300 Acetopromazine Aniline Phencyclidine 50,000 N-Acetyl-L-cysteine Antipyrine OPIATE (OPI 300,MOR,MOP) 50,000 N-Acetylprocainamide (Acedainide ) Acetylsalicylic Acid ( Aspirin L-Ascorbic Acid 6-acetylmorphine 500 100,000 Albumin, standard ASP-PHE-Methyl-Ester ( Aspartame) 100,000 Codeine 100 (Diallybarbituric Acid ) D-Aspartic Acid Eserine (Physostigmine) 15,000 hydrochloride 100,000 Allopurinol ( 4-Hydroxypyrazole(3,4- pyrimidine ) DL-Aspartic Acid Ethylmorphine 100 L–Aspartic Acid Heroin 500 6-Acetylmorphine（6-ACM） (Adamantan-1-amine ) Amcinonide Barbituric Acid Hydromorphone 6-Acetylmorphine 10 2,000 Amikacin Beclomethasone Morphine 40 Hydrocodone 1,250 Beclomethasone Dipropionate Morphine 300 Bilirubin 3,500 p-Aminobenzoic Acid Bendroflumethiazide Benzidine Histamine Nialamide Quinolinic Acid Aniline Indapamide Probenecid Tricyclic Antidepressants Non Benzilic Acid diethylaminoethyl ester Dichlorphenamide DL- (+/-) Ranitidine Antipyrine Indomethacin Cross-Reacting Compounds Diclofenac Hydrastine Nicotinic Acid Rescinnamine DL-Amphetamine sulfate Ipratropium *Parent compound only: Benzoic Acid Dicyclomine Hydrochlorothiazide DL-Aspartic Acid Iproniazid Dieldrin Hydrocortisone Nitrofurantoin Riboflavin L–Aspartic Acid Isonicotinic Acid Promazine 4-Acetamidophenol Benzthiazide Diflorasone Diacetate Hydrocarbalamine Apomorphine Promethazine Acetophenetidin Benztropine Diflucortolone pivalate Hydroflumethiazide Norclomipramine (Albuterol ) Aprobarbital Propionylpromazine N-Acetylprocainamide Benzyl alcohol Diflunisal Hydroxyhippuric Acid Salicylic Acid Aspartame Kanamycin Acetylsalicylic acid Benzylamine Digitoxin Norcodeine Sodium Chloride Aminopyrine Digoxin Nordoxepin Sodium Formate Barbituric Acid Ketoprofen Pyridine-2-Aldoxime Amobarbital Betamethasone Dihydroxymandelic Acid Indapamide Norethindrone Sulfamethazine Benzidine Pyridoxine Amoxicillin Bilirubin Indomethacin Norfloxacin Sulfamethoxazole Benzilic Acid Benzocaine Pyrilamine DL-Amphetamine Normorphine Sulfanilamide Benzoic Acid Levorphanol Ampicillin Iproniazid Sulfathiazole Benzoylecgonine Quinine Ascorbic acid Dimethylaminoantipyrin Isonicotinic Acid Nylidrin Sulfisoxazole Benzphetamine Meperidine Quinolinic Acid Apomorphine Dimethyl Isosorbide Isopropamide Sulindac Benzthiazide Oxazepam Aspartame Dimethyl Sulfoxide Isoxsuprine Oxalic Acid Bilirubin Ranitidine Atropine Butyrophenone Kanamycin Tannic Acid Bisacodyl Hydroxyprogesterone Rescinnamine Benzilic acid Caffeine Dipyridamole Ketamine Bromazepam (Ritalin ) Reserpine Benzoic acid Camphor Dipyrone Ketoprofen 2-Bromo-a -ergocryptine Methyl Salicylate Riboflavin Benzoylecgonine Canrenoic Acid Kynurenic Acid Oxyphenbutazone Brompheniramine Nabumetone Ritodrine Benzphetamine Doxepin Labetalol Oxypurinol Theobromine Caffeine Salbutamol (Albuterol ) Bilirubin Doxycycline Levorphanol Theophylline Cannabidiol Salicylic Acid Brompheniramine Carbamyl-Carboplatin Doxylamine Thiamine Cannabino Nalidixic Acid Secobarbital Caffeine Lisinopril Papaverine Chloramphenicol Nalmefene Sodium Chloride Cannabidiol Cefaclor Ecgonine Lithium Carbonate Pargyline Tobramycin Chlorcyclizine (+/-) Nicotine Sodium Formate Cannabinol Cefadroxil Ecgonine Methyl Ester Loperamide Penicillin Chlordiazepoxide Nicotinic Acid Sulfamethazine Chloralhydrate Cefotaxime Emetine Pentachlorophenoll Chloroquine Nifedipine Sulfamethoxazole Chloramphenicol Cefoxitin Lysergic Acid Diethylamide (LSD) Pentoxifylline Tolmetin Chlorothiazide Nitrazepam Sulfanilamide Chlordiazepoxide Ceftriaxone Epinephrine Mebendazole Pentylenetetrazole Chlorotrianisene Noscapine Sulfathiazole Chlorothiazide Cefuroxime p-Phenylenediamine Chlorpheniramine Oxycodone Sulfisoxazole (±) Chlorpheniramine Cephalexin Eserine Phenelzine Triamcinolone Oxymetazoline Sulindac Chlorpromazine Estradial Phenformin Dimercaprol Oxyphenbutazone Talbutal Chlorquine Cephradine Estriol Trichlormethiazide Dimethylaminoantipyrin Oxypurinol Tamoxifen Chloramphenicol Estron Melanin Trichloroacetic acid Dimethyl Isosorbide Paclitaxel Tannic Acid Chlorcyclizine Glucuronide Melphalan Phenolphthalien Dimethyl Sulfoxide Pancuronium Bromide Tenoxicam Cocaine hydrochloride Chloroquine Estrone-3-Sulfate Disopyramide Papaverine Terbutaline Codeine Chlorothiazide Ethacrynic Acid Meperidine Phenoxymethyl Trimethoprim Dobutamine Pargyline Terfenadine Cortisone Chlorotrianisene Ethambutol Mephenesin Penicillinic acid ( Penicillin V ) Trimipramine Doxepin Penicillin Tetracycline (-) Chlorpheniramine Ethamivan Mephentermine Doxycycline Pentachlorophenol Tetraethylthiuram Creatinine Chlorpromazine Ethanol, Standard Phenylbutazone Tropic Acid Ecgonine Pentobarbital Tetrahydrozoline Deoxycorticosterone Ethopropazine Metaproterenol Phenylethylamine Tropine Ecgonine Methyl Ester Pentoxifylline Theobromine Phenylnalonamide Tryptamine Emetine Pentylenetetrazole Theophylline Diazepam Chlorthalidone Glycol Methadone Ephedrine p-Phenylenediamine Thiamine Diclofenac Chlorzoxazone Ethylenediamine Tetraacetic Acid , Absolute Picrotoxin Urea ( Carbamide ) Epinephrine Phenelzine Thioridazine Diflunisal Cholesterol Etodolac Uric Acid Erythromycin Phenformin Tobramycin Digoxin Cimetidine Etoposide Methazolamide Vancomycin Estriol Pheniramine Tolazamide Diphenhydramine Cinchonidine Famotidine Methotrimeprazine Vincamine Estrone Phenobarbital Tolbutamide Doxylamine Cinoxacin Ethyl-p-aminobenzoate Phenol Tolmetin Ecgonine hydrochloride Fenoprofen Naphthalene Acetic Acid Pipecolic Acid Etodolac Phenolphthalien Toluene Ecgonine methylester Naproxen Pipedemic Acid Zearalenone Etoposide Phenothiazine Trazodone (IR,2S)-(-)-Ephedrine Ferrous Sulfate Methoxyamine Piroxicam Zomepirac Famotidine Phenoxymethyl Triamcinolone L-Ephedrine Clobetasone Butyrate Methoxyphenamine Potassium Chloride Fenfluramine Penicillinic acid ( Penicillin V ) Triamterene (-) Y Ephedrine Clomipramine Flunisolide Hydroxyprogesterone Potassium Iodide Ferrous Sulfate Phentolamine Triazolam Erythromycin Clonidine Methylene Blue Flufenamic Acid Phenylbutazone Trichlormethiazide β-Estradiol Cloxacillin Flurandrenolide Methylphenidate (Ritalin ) Flunisolide Phenylethylamine Trichloroacetic acid Estrone-3-sulfate Methyl Salicylate Prednisone Methadone Non Cross-Reacting Formaldehyde Phenylpropanolamine Trifluoperazine Compounds Ethyl-p-aminobenzoate Colchicine Flurbiprofen Meticrane Phenyltoloxamine Triflupromazine Fenoprofen Cortisone Formaldehyde Metronidazole Primaquine *Parent compound only： Gemfibrozil Picrotoxin Trimethobenzamide Furosemide Cortol Furosemide Milrinone Gentamicin Sulfate Pilocarpine Trimethoprim Gentisic Creatinine Gemfibrozil Minaprine Proadifen Gentisic Acid Pimozide Trimipramine Hemoglobin Cromolyn Gentamicin Sulfate Nabumetone Probenecid Acebutolol Glucose Pinacidil Triprolidine Hydralazine Gentisic Acid Nadolol Procainamide Acetaldehyde Hemoglobin Pindolol Tropic Acid Hydrochlorothiazide Glucose Nafcillin Prochlorperazine Acetaminophen Acetazolamide Hydralazine Pipecolic Acid Tropine Hydrocodone Cyclosporin A Glybenclamide Nalbuphine Procyclidine Acetone Hydrastine Pipedemic Acid Tryptamine Hydrocortisone Nalidixic Acid Promazine Acetophenetidin Hydrochlorothiazide Piroxicam Tyramine p-Hydroxyamphetamine Dantrolene Guaiacol Glyceryl Ether Nalmefene Promethazine N-Acetylprocainamide (Acedainide ) Hydrocodone Potassium Chloride Urea ( Carbamide ) O-Hydroxyhippuric Deferoxamine Mesylate Guanethidine Nalorphine Propionylpromazine Acetylsalicylic Acid ( Aspirin) Hydrocortisone Potassium Iodide Uric Acid p-Hydroxy-methamphetamine PN: Halcinonide Naloxone Protriptyline Aminopyrine Hydrocarbalamine Prazepam Vancomycin 3-Hydroxytyramine Desipramine Naltrexone Pseudoephedrine Amitryptyline Hydroflumethiazide Prazosin Vincamine Y03 11 109905 Ibuprofen Desmethyldiazepam Hemoglobin - Pyridine-2-Aldoxime Ammonium Chloride Hydroxyhippuric Acid Prednisone Xylometazoline Iproniazid Desoximetasone Hexachlorocyclohexane Naphthalene Acetic Acid Pyridoxine Amobarbital p-Hydroxyamphetamine Prilocaine Yohimbine (-) Isoproterenol Hexachlorophene Naphthol Pyrilamine Amoxicillin Hydroxyzine Primaquine Zearalenone Isoxsuprine Dextromethorphan Neomycin Sulfate Quinidine Amphotericin B Ibuprofen Primidone Zomepirac Ketamine Hippuric Acid Niacinamide Quinine Ampicillin Imipramine Proadifen Zopiclone Ketoprofen Labetalol Trimethoprim Haloperidol Ampicillin Hydralazine Hydrochloride Promazine Hydrochloride Theophylline Levorphanol D, L-Tryptophan Hemoglobin Amitriptyline Hydrochloride Hydrocodone Promethazine Thioridazine Loperamide Tyramine Hydralazine S(-)-Amphetamine α-Hydroxyhippuric Acid D-Propoxyphene Thiamine, (Vitamin B1) HCL Meperidine D, L- Hydrocortisone R(-)-Amphetamine 21-Hydroxyprogesterone Hydrochloride L-Thyroxine Meprobamate Uric acid Hydroxycarbalamine Amobarbital p-Hydroxymethamphetamine Pseudoephedrine HCL Tolbutamide Methadone Hydroxyprogesterone (±)Amphetamine Hydrocortisone Quinine Trimethoprim D-methamphetamine Zomepirac Hydroxyzine R-(-)-Apomorphine Hydrochloride Hemihydrate Hydrochlorothiazide Quinidine Trazodone Hydrochloride Methoxyphenamine Ibuprofen Aspirin (±)- 4-Hydroxyamphetamine HCL Quinacrine Dihydrochloride Triamterene Methylenedioxymethamphetamine 3,4-Methylene-dioxyethylamp Indomethacin Aspartame Ibuprofen Ranitidine Hydrochloride Trimipramine hetamine Non Cross-Reacting Compounds Lidocaine L-Ascorbic Acid Imipramine HCL Salicylic Acid Tryptamine (+)3,4-Methylene-dioxymetha *Parent compound only Lisinopril Atropine Iprazid Secobarbital Trifluoperazine Dihydrochloride mphetamine Lithium Benzphetamine HCL Isoxsuprine Hydrochloride Serotonin HCL DL-Tryptophan Methylphenidate Acetaldehyde Loperamide Benzilic Acid Isoproterenol Hydrochloride HCL Trans-2-Phenylcyclopropylamine Morphine-3-β-D-glucuronide Acetaminophen Lorazepam Benzoylecgonine Ketamine Hydrochloride Sulfamethazine Hydrochloride Morphine sulfate Acetazolamide LSD SS Benzoic Acid Ketoprofen Sulindac DL-Tyrosine Nalidixic acid Acetone Metronidazole Bilirubin, Mixed Isomers Labetalol Hydrochloride Tyramine Naloxone Albumin Naproxen Temazepam Brompheniramine Maleate Levorphanol Naltrexone Albuterol Niacinamide Tetracycline Uric Acid Buspirone Hydrochloride Loperamide Hydrochloride Naproxen Ammonium Nicotine Tetrahydrozoline Hydrochloride Verapamil Hydrochloride Butabarbital Succinate Salt Niacinamide Amphotericin B Nifedipine Tetrahydrocortisone 3-(β-D-Glucuronide) Zomepirac Sodium Salt Cannabidiol Maprotiline Hydrochloride Nifedipine Ampicillin Nitrofurantoin Thebaine Norcodein Amtriptyline Nortriptyline Cannabinol (±)-3,4-Methylenedioxyethylamphetamine Norethindrone Apomorphine Ofloxacin Caffeine (±)-3,4-Methylenedioxyamphetamine The Other Few Non D-Norpropoxyphene Ascorbic Acid Oxalic Acid Chlordiazepoxide HCL Meperidine Cross-Reacting Compounds Noscapine Aspartate Penicillin G Chlorothiazide Meprobamate of BUP at Concentration of D,L- Aspirin Pentobarbital Chloroquine Diphosphate Methamphetamine Hydrochloride 100μg/mL: Oxalic acid Phenobarbital Chlorpheniramine Maleate (±)Methadone Oxazepam Atropine Chlorpromazine Hydrochloride S(+)-Methamphetamine Codeine Oxolinic acid Beclomethasone Prednisone Chloramphenicol L-methamphetamine Morphine Oxycodone Benzocaine Prochloperazine Hydrate Methoxyphenamine Hydrochloride Cholesterol Methylphenidate Oxymetazoline Benzoic Acid Promethazine BIBLIOGRAPHY Papaverine Bilirubin Propoxyphen Chlorothiazide (±)-3,4-Methylenedioxymethamphetamine Penicillin-G Propranolol Clomipramine Hydrochloride 1. 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Drug Testing in the workplace, 114. Trans-2-Phenyl-cylopropylami Chlordiazepoxide Sidenafil(Viagra) Dextromethorphan (Vitamin B3) 7. Baselt RC. Disposition of Toxic Drugs and Chemicals in Man. 2nd Ed. Biomedical Publ., Davis, CA 1982; 487. ne-hydrochloride Chloroquine Sodium Chloride Diazepam 8. OSHA, The Bloodborne Pathogens Standard 29, Code of Federal Regulations 29 CFR 1910.1030. β-Phenylethlamine Chlorothiazide Sulfamethoxazole Diclofenac Sodium Nifedipine 9. CDC, Centers for Disease Control (CDC) Guidelines, Morbidity and Mortality Weekly Report, Volume 37, Phenylpropanolamine Chlorpheniramine Sulindac Number 24, 1988. Dicyclomine Norcodeine 10. Ethyl Glucuronide: An unusual Ethanol Metabolite in Humans. Synthesis, Analytical Data, and Determination in Prednisolone Chlorpromazine Temazepam Diflunisal Nordoxepin Hydrochloride Serum and Urine. Schmitt G., et al. Journal of Analytical Toxicology. 1995, 19:91-94. Prednisone Chlorpropamide Tetracycline Digoxin Norethisterone 11. Comparison of Urinary Excretion Characteristics of Ethanol and Ethyl Glucuronide. Dahl H., et al. Journal of Procaine Cholesterol Tetrahydrocortisone 4-Dimethylaminoantipyrine D-Norpropoxyphene Maleate Salt Analytical Toxicology. 2002, 26:201-204. Promethazine Clindamycin Theophyline 5,5-Diphenylhydantoin Noscapine HCL Hydrate 12. Ethyl Glucuronide- the direct ethanol metabolite on the threshold from science to routine use. Wurst FM et al. D,L-Propanolol Clonidine Thiamine Diphenhydramine Noroxymorphone HCL Addiction. 2003, 98 (S2) 51-61. D-Propoxyphene Clozapine Thioridazine Dopamine Hydrochloride Nylidrin Hydrochloride 13. Ethyl Glucuronide- A Biological Marker for recent alcohol consumption. Seidi S. et al. Addiction Biology. 2001, D-Pseudoephedrine Colchicine Thyroxine Doxylamine Succinate Salt (±)-Octopamine HCL 6(3):205-212. 14. Ethyl Glucuronide: A Biomarker to identify Alcohol use by Health Professionals Recovering from Substance Quinidine Cortisone Tobutamide Ecgonine Methyl Ester Oxalic Acid use Disorders. Skipper G.E et al. Alcohol and Alcoholism, 2004, 39(5):445-449. Quinine Creatinine Trazodone Ecgonine HCL Oxazepam Deoxycorticosterone 15. Ethyl Glucuronide- A marker of Recent Alcohol Consumption with Clinical and Forensic Implications. Wurst FM Ranitidine Trimethoprim Oxolinic Acid Salicylic acid Desipramine Tryptophan et al. Alcohol. 2000, 20(2):111-116. Emetine Dihydrochloride Hydrate Oxycodone 16. Ethyl Glucuronide (EtG): A new marker to detect Alcohol use in recovering physicians., Skipper G.E., et al. Secobarbital Dextromethorphan Tyrosine (-)-Epinephrine Oxymetazoline Hydrochloride Journal of Medical Licensure and Discipline. 2004, 90(2): 14-17. Serotonin Diazepam Urea Ephedrine-(±) Hydrochloride Papaverine Hydrochloride 17. Production of urinary ethanol after sample collection. Saady, J.J., Poklis, A.and Dalton, H.P. (1993) Journal of (5-Hydroxytyramine) Digoxin Uric Acid (-)-Ephedrine HCL Phencyclidine Forensic Sciences 38, 1467-1471. Sulfamethazine Diphenhydramine Valproic Acid (1R,2S)-(-)-Ephedrine Pentobarbital 18. Preliminary immunochemical test for the determination of Ethyl Glucuronide in serum and urine: Comparison of Sulindac Dipyridamole Verapamil Erythromycin Pentazocine screening method results with Gas Chromatography- Mass spectrometry. Zimmer H., et al. Journal of Analytical Temazepam Doxycycline Zoloft Toxicology. 2002, 26:11-16. Estradiol Perphenazine Tetracycline Erythromycin 19. Confirmatory Analysis of Ethyl Glucuronide in urine by liquid chromatography/ElctrosprayIonization/Tandem Tetrahydrocortisone, 3 Estradiol Ethyl Glucuronide Estrone-3-Sulfate Potassium Salt Penicillin G Sodium Salt Mass Spectrometry according to forensic guidelines. Weinmann W. et al. J. Am. Soc. Mass Spectrom. 2004, Acetate Estriol Non Cross-Reacting Compounds Ethyl-P-Aminobenzoate Phenelzine Sulfate Salt 15(2):188-193. Tetrahydrocortisone 3 Estrone *Parent compound only: Fenoprofen Calcium Salt Hydrate Phenobarbital (β-D-glucuronide) Ethanol Furosemide Phentermine HCL Manufactured for: Tetrahydrozoline Ethylene Glycol Acebutolol Hydrochloride Gentisic Acid Phenylethylamine W.H.P.M., Inc. Thiamine Epinepherine -d6 Hydrochloride D-Glucuronic Acid L- 5358 Irwindale Ave. Thioridazine Ferrous Sulfate Acetaminophen Phenylpropanolamine Hydrochloride Irwindale, CA 91706 Tolbutamine Furosemide N-Acetylprocainamide Guaifenesin (Guaiacol Glyceryl Ether) Prednisolone www.whpm.com Triamterene Gentamycin Acetophenetidin Hemoglobin Porcine Prednisone Acetate Trifluoperazine Glucose Amoxicillin Hippuric Acid Procaine HCL Effective date: 06/29/2016