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Molecular Insights of Mitochondrial 16S Rdna Genes of the Native Honey Bees Subspecies Apis Mellifera Carnica and Apis Mellifera Jementica (Hymenoptera: Apidae) In
Molecular insights of mitochondrial 16S rDNA genes of the native honey bees subspecies Apis mellifera carnica and Apis mellifera jementica (Hymenoptera: Apidae) in Saudi Arabia Reem Alajmi1, Rewaida Abdel-Gaber1,2*, Loloa Alfozana1 1Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia 2Faculty of Science, Department of Zoology, Cairo University, Cairo, Egypt Corresponding author: Rewaida Abdel-Gaber E-mail: [email protected] Genet. Mol. Res. 18 (1): gmr16039948 Received Nov 30, 2018 Accepted Dec 21, 2018 Published Jan 05, 2019 DOI: http://dx.doi.org/10.4238/gmr16039948 Copyright © 2018 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution ShareAlike (CC BY-SA) 4.0 License. ABSTRACT. The honey bee Apis mellifera is of major importance for the world’s agriculture and is also suitable for environmental monitoring. It includes several recognized subspecies distinguished by using morphological and morphometric variants. Here, 200 adult worker Apis mellifera honey bees were collected from Hail region, Saudi Arabia. Mitochondrial 16S rDNA was conducted to detect molecular polymorphism among honey bee A. mellifera subspecies. The amplified and sequenced gene regions of mtDNA revealed the presence of two different subspecies of Apis mellifera carnica (gb| MH939276.1) and Apis mellifera jementica (gb| MH939277.1). The sequences were compared with each other and with others retrieved from the GenBank demonstrating a high degree of similarity (up to 72%). The NJ tree indicated that all Apis species are clustered together in one clade in addition to the genetically origin of Apis species within family Apidae as a paraphyletic group within the African lineage. -
The Controllable Bee Hive and New System of Bee Management: 1887 Annual Circular
The University of Maine DigitalCommons@UMaine Maine History Documents Special Collections 1887 The Controllable Bee Hive and New System of Bee Management: 1887 Annual Circular Lizzie E. Cotton Follow this and additional works at: https://digitalcommons.library.umaine.edu/mainehistory Part of the History Commons This Monograph is brought to you for free and open access by DigitalCommons@UMaine. It has been accepted for inclusion in Maine History Documents by an authorized administrator of DigitalCommons@UMaine. For more information, please contact [email protected]. Pamp 88 i Mrs. Lizzie E. Cotton, WEST GORHAM, MAINE. the CONTROLLABLE BEE HIVE ------•—^-AND—j—•------ NEW SYSTEM Of BEE MANAGEMENT. In bringing this my Annual Circular for 1887 before the public, I wish to advise every intelli gent person who has a spot of land on which to set a hive, to keep bees. Managed on my plan in Controllable Hives, bees are more profit than any thing connected with the farm or garden. Bees will go many miles in all directions from their hives to collect honey. The sources from which bees collect honey are almost innumerable. Nearly every flower, plant, tree, shrub and vine in field, forest, garden and pasture yield honey. If it is not desired to enter largely into the production of honey for market, it is certainly very desirable to keep one or two hives of bees to produce honey for family use. There is no greater health giving lux ury than pure honey in snow white comb. Severe hoarseness, sore throats, coughs, and many other diseases are cured by the use of pure honey. -
Life Cycles: Egg to Bee Free
FREE LIFE CYCLES: EGG TO BEE PDF Camilla de La Bedoyere | 24 pages | 01 Mar 2012 | QED PUBLISHING | 9781848355859 | English | London, United Kingdom Tracking the Life Cycle of a Honey Bee - dummies As we remove the frames, glance over the thousands of busy bees, check for brood, check for capped honey, maybe spot the queen… then the frames go back in their slots and the hive is sealed up again. But in the hours spent away from our hives, thousands of tiny miracles are happening everyday. Within the hexagonal wax cells little lives are hatching out and joining the hive family. The whole process from egg to adult worker bee takes around 18 days. During the laying season late spring to summer the Queen bee is capable of laying over eggs per day. Her worker bees help direct her to the best prepared comb and she lays a single egg in each hexagon shaped cell. The size of the cell prepared determines the type of egg she lays. If the worker bees have prepared a worker size cell, she Life Cycles: Egg to Bee lay a fertilized egg. This egg will produce a female worker bee. If the worker bees have prepared a slightly larger cell, the queen will recognize this as a drone cell and lay an unfertilized egg. This will produce a male drone bee. It is the workers and not the queen that determine the ratio of workers to drones within the hive. In three days the egg hatches and a larva emerges. It looks very similar to a small maggot. -
Friedrich Ruttner Biogeography and Taxonomy of Honeybees
Friedrich Ruttner Biogeography and Taxonomy of Honeybees With 161 Figures Springer-Verlag Berlin Heidelberg GmbH Professor Dr. FRIEDRICH RUTTNER Bodingbachstraße 16 A-3293 Lunz am See Legend for cover mOlif: Four species of honeybees around the area of distribution. ISBN 978-3-642-72651-4 ISBN 978-3-642-72649-1 (eBook) DOI 10.1007/978-3-642-72649-1 Library of Congress Cataloging in Publication Data. Ruttner, Friedrich. Biogeogra phy and taxonomy of honeybees/Friedrich Ruttner. p. cm. Bibliography: p. In c\udes. index. 1. Apis (Insects) 2. Honeybee. I. TitIe. QL568.A6R88 1987 595.79'9--dc19 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, re-use of illustrations, recitation, broadcasting, reproduction on microfilms or in other ways, and storage in data banks. Duplication of this publication or parts thereof is only permitted under the provisions of the German Copyright Law of September 9, 1965, in its version of lune 24, 1985, and a copyright fee must always be paid. Vio lations fall under the prosecution act of the German Copyright Law. © Springer-Verlag Berlin Heidelberg 1988 Originally published by Springer-Verlag Berlin Heidelberg New York in 1988 Softcover reprint of the hardcover 18t edition 1988 The use of registered names, trademarks, etc. in this publication does not imply, even in th absence of a specific statement, that such names are exempt from the relevant prutective laws and regulations and therefore free for general use. Data conversion and bookbinding: Appl, Wemding. -
PREVALENCE of WOLBACHIA PIPIENTIS and VARROA DESTRUCTOR MITES in AFRICANIZED BEES in the DEMING, NEW MEXICO AREA Niccole D
International Journal of Science, Environment ISSN 2278-3687 (O) and Technology, Vol. 9, No 2, 2020, 274 – 284 2277-663X (P) PREVALENCE OF WOLBACHIA PIPIENTIS AND VARROA DESTRUCTOR MITES IN AFRICANIZED BEES IN THE DEMING, NEW MEXICO AREA Niccole D. Rech1*, Alea Darrow2, Eliza Lopez3, Daniel Mendoza4, Viviana Nicoll5 and Lauren Paulk6 1Western New Mexico University, Deming, New Mexico 2,3,4,5,6Early College High School Deming, New Mexico E-mail: [email protected] (*Corresponding Author) Abstract: Africanized Honeybees (AHB) were colonized in New Mexico by 1992. Since then, they have virtually replaced Western honeybees in Luna County, which is adjacent to Mexico’s Northern border. AHB are hybrids between Apis mellifera scutellata, an African honeybee, and A. m. ligustica, A.m.iberiensis, which are both Western honeybees. Wolbachia pipientis is a Rickettsial endosymbiont bacterium that infects arthropods and nematodes. W. pipientis infestation can manipulate the reproduction of arthropods causing cytoplasmic incompatibility, feminization, parthenogenesis, sterilization, and male killing which decreases the number of progeny and skews the male/ female ratios in arthropod populations. Varroa destructor mites also impact honeybee populations by spreading viral infections such as the deformed wing virus (DWV). Honeybees, Africanized or not, are the main pollinators of many crops grown in Southern New Mexico and are suppliers of honey, royal jelly, wax, and bee venom. A decline in honey bee populations impacts the entire agricultural industry of New Mexico. In this study 88 AHB were tested for W. pipientis and 92 AHB were inspected for V. destructor mites. Twenty-six percent of the bees were infected with W. -
Backyard Beekeeping Providing Pollinator Habitat One Yard at a Time
Backyard Beekeeping Providing pollinator habitat one yard at a time By Nichelle Harriott A Beyond Pesticides Factsheet n light of growing concern over the recent loss and overcome, many find bees a welcome addition to their backyards. disappearance of bees and bee colonies across the country, If you are interested in encouraging bees to visit your yard, the Imany backyard enthusiasts are rediscovering a relatively following tips will ensure that you and your bees live happily simple and fun way to assist these essential pollinators. Attracting together. and keeping bees in your backyard can be easy, especially if you already enjoy gardening. By providing bee habitat in your yard, Create a Bee Garden you can increase the quality and quantity of your garden fruits and vegetables. n Bee Colorful! Bees are attracted to most flowering plants, and are especially The United States is home to a variety of bees species. Bumblebees, fond of blue and yellow flowers. Other colors such as purple, carpenter bees, sweat bees, leafcutter bees, digger bees are just white and pink also serve to attract bees. Make sure there are some of thousands of bees in the U.S. Most of them are solitary, plants that will flower during different parts of the season to keep friendly bees that nest in holes in the ground or burrows in twigs your garden flourishing throughout the summer and well into and dead tree limbs. fall. This serves to provide a steady supply of nectar and pollen for bees. A diversity of flowers planted is close proximity to each While many may prefer butterflies and birds to pay a visit to their other strongly attract bees. -
Thermoregulation in Colonies of Africanized and Hybrids with Caucasian, Italian and Carniolan Apis Mellifera Honey Bees
Thermoregulation in Colonies of Africanized and Hybrids With Caucasian, Italian and Carniolan Apis mellifera Honey bees Vagner de Alencar Arnaut deToledo1* and Regina Helena Nogueira-Couto2 1Department of Animal Science at the Universidade Estadual de Maringá, Colombo, Av. 5790, 87020-900, Maringá (PR) Brazil. 2Faculdade de Ciências Agrárias e Veterinárias (FCAVJ), Jaboticabal - UNESP - Rod. Carlos Tonanni, km5, 14870-000, Jaboticabal (SP) Brazil. ABSTRACT This experiment was carried out to study the internal temperature regulation of a colony of Africanized honey bees (AFR), compared with hybrid Caucasian (CAU), Italian (ITA), and Carniolan (CAR) bees, during the period of one year and different size hives located in a sub-tropical region. The instant internal temperature, 33.7 ± 1.5°C for the AFR, 33.5 ± 1.4°C for the CAU, 33.7 ± 1.5°C for the ITA and 33.8 ± 1.4°C for the CAR, did not show any significant difference (P>0.05). The maximum temperature (36.1 ± 2.3°C) was statistically different (P<0.05) from the minimum (27.6 ± 5.3°C). There was no difference (P>0.05) in the mean internal temperature, between the nucleus (31.7 ± 6.3°C) and the brood nest (32.1 ± 5.3°C) measured between two and four o'clock in the afternoon. Key words: Temperature, honey bee, Apis, thermoregulation, hive, hybrids INTRODUCTION Büdel (1955) found, in areas with egg-larvae, higher temperatures than in areas with pupae, Thermoregulation in Apis mellifera colonies occurs which in turn, had higher temperatures than those due to the clustering of large quantities of bees, observed in empty cells. -
Morphological Study of Honeybees (Apis Mellifera) from Libya*
Apidologie 40 (2009) 97–105 Available online at: c INRA/DIB-AGIB/ EDP Sciences, 2009 www.apidologie.org DOI: 10.1051/apido/2008068 Original article Morphological study of Honeybees (Apis mellifera) from Libya* Taher Shaibi1,StefanFuchs2, Robin F.A. Moritz1 1 Institut für Biologie, Martin-Luther-Universität Halle-Wittenberg Hoher Weg 4, 06099 Halle/Saale, Germany 2 Institut für Bienenkunde Fachbereich Biologie der J. W. Goethe-Universität Frankfurt am Main Karl-von-Frisch-Weg 2, 61440 Oberursel, Germany Received 21 November 2007 – Revised 6 September 2008 – Accepted 13 October 2008 Abstract – We show, with classical morphometrical analyses, that Libyan honeybees sampled at coastal and desert locations are distinctly different from both the adjacent A. m. intermissa bee populations of Tunisia and Algeria and those of A. m. lamarckii of Egypt. The morphotype was most closely related to A. m. sahariensis and, based on wing venation angles, showed affinities to A. m. jemenitica, indicating that the sampled populations might be derived from a formerly extended Saharan honeybee population during the Holocene pluvial. Scattered morphometric similarities to the European bee A. m. ligustica suggest that importation of honeybees from Italy may have had only minor impact on endemic Libyan honeybee popu- lations. Conservation measures might be particularly appropriate for remote oasis populations, which might be true relic population from the Holocene. Apis mellifera / morphometry / Libya / sahariensis / conservation 1. INTRODUCTION 2001; Whitfield et al., 2006). Nevertheless, the subspecies of northwestern Africa and some Apis mellifera L. is widespread in Africa, populations of A. m. iberica were assigned to Europe and parts of Asia with a wide di- Branch A instead of M, whereas A. -
Beekeeper Volume I, II & III Ebook Beekeeping Naturally
The Practical Beekeeper Volume I, II & III EBook Beekeeping Naturally Copyright © 2004-2011 by Michael Bush All rights reserved. No part of this book may be reproduced or transmitted in any form or by any means without written permission of the author. Cover Photo © 2011 Alex Wild www.alexanderwild.com ISBN: 978-161476-084-9 X-Star Publishing Company Founded 1961 Dedication This book is dedicated to Ed and Dee Lusby who were the real pioneers of modern natural beekeeping methods that could succeed with the Varroa mites and all the other new issues. Thank you for sharing it with the rest of us. About the Book This book is about how to keep bees in a natural and practical system where they do not require treatments for pests and diseases and only minimal interventions. It is also about simple practical beekeeping. It is about reducing your work. It is not a main-stream beekeeping book. Many of the concepts are contrary to “conventional” beekeeping. The techniques presented here are streamlined through decades of experimentation, adjustments and simplification. The content was written and then refined from responding to questions on bee forums over the years so it is tailored to the questions that beekeepers, new and experienced, have. It is divided into three volumes and this edition contains all three: Beginning, Intermediate and Advanced. Acknowledgments I’m sure I will forget to list many who have helped me along this path. For one thing many were often only known by the names they used on the many bee forums where they shared their experiences. -
A Comparison of Russian and Italian Honey Bees
A Comparison of Russian and Italian Honey Bees American beekeepers have been using Italian honey bees (Apis mellifera ligustica) since they were first imported to the New World in 1859. The standard German honey bee (A. m. mellifera), which had been in America for more than 200 years, was by that time ill-tempered, disease-ridden, and less suited for honey bee management. Conversely, the Italian bees were and are excellent honey producers, show a gentle temperament that makes them the most popular race of honey bee in North America, have a moderately low tendency to swarm, and have a bright yellow color that makes queens easy to find. ut Italian honey bees are suscep- chemicals can contaminate the honey Btible to two deadly parasitic mites, and beeswax in a hive. The mites also the tracheal mite (Acarapis woodi) and are becoming increasingly resistant to the varroa mite (Varroa destructor), the pesticides, making the chemicals which were introduced into the U.S. in less reliable and, eventually, ineffec- 1984 and 1987, respectively. Colonies tive. The high colony mortality that ac- contract these mites through equipment companies these two mites is a serious sharing and overcrowding, and, once concern of the bee industry today, and infested, entire colonies can succumb various types of bees are continually within one or two years. Beekeepers being examined with an eye toward have relied largely on pesticides to finding a hardy, productive stock that control the mites, but many of these can resist them. Russian Bee Project Management of Russian bees Efforts to find a honey bee that is genetically resistant to Russian bees are quite different from standard Italian bees the varroa and tracheal mites led researchers at the USDA in several ways (Table 1): Honey Bee Research Laboratory in Baton Rouge, Louisi- • Russian bees do not build their colony populations ana, to Russia. -
Defensive Behavior of Africanized Honeybees (Hymenoptera: Apidae) in Dourados-Mato Grosso Do Sul, Brazil
Revista Colombiana de Entomología 40 (2): 235-240 (Julio - Diciembre 2014) Defensive behavior of africanized honeybees (Hymenoptera: Apidae) in Dourados-Mato Grosso do Sul, Brazil Comportamiento defensivo de las abejas africanizadas (Hymenoptera: Apidae) en Dourados-MS, Brasil MÁRCIA REGINA FAITA1, RITA MARIA MATTOSO COLMAN CARVALHO2, VALTER VIEIRA ALVES-JUNIOR1,2 and JOSÉ CHAUD-NETTO3 Abstract: African bees were introduced in Brazil in 1956, in an attempt to improve honey production. The accidental hybridization between African and European breeds originated africanized bees, which are very well adapted to the local climate. That bee poly-hybrid has an initial production of honey 70 % more than Europeans. However, African and africanized bees were much more defensive than European subspecies, which required the development of appropriate management techniques. Beekeepers in southern Mato Grosso do Sul learned to work with africanized bees. The aim of this study was to evaluate the defensive behavior of Africanized bees in Dourados MS, using a ball of black leather and artificial enemy. There were is recorded, the time to deliver the first bite, the time it takes to enrage (attack the enemy with great intensity), the distance from the pursuit of the enemy after the first attack and the number of bites left in the beanbag. The results indicate a significant concentration of colonies of bees with different intensity and defensive behavior, but similar to that presented by africanized bees in the 60/70, particularly in the region of Ribeirão Preto (SP). It appears, in terms of their defensive behavior, bees in the southern region of the state, did not suffer influence by bees of European origin. -
GENETIC VARIATION in HONEY BEES from an AREA of RACIAL HYBRIDIZATION in WESTERN CZECHOSLOVAKIA Walter S
GENETIC VARIATION IN HONEY BEES FROM AN AREA OF RACIAL HYBRIDIZATION IN WESTERN CZECHOSLOVAKIA Walter S. Sheppard, Bruce A. Mcpheron To cite this version: Walter S. Sheppard, Bruce A. Mcpheron. GENETIC VARIATION IN HONEY BEES FROM AN AREA OF RACIAL HYBRIDIZATION IN WESTERN CZECHOSLOVAKIA. Apidologie, Springer Verlag, 1986, 17 (1), pp.21-32. hal-00890673 HAL Id: hal-00890673 https://hal.archives-ouvertes.fr/hal-00890673 Submitted on 1 Jan 1986 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. GENETIC VARIATION IN HONEY BEES FROM AN AREA OF RACIAL HYBRIDIZATION IN WESTERN CZECHOSLOVAKIA Walter S. SHEPPARD Bruce A. McPHERON Department of Entomology, University of Illinois 320 Morrill Hall, SOS S. Goodwin Ave. Urbana, Illinois 61801 SUMMARY Twelve colonies of honey bees from an area of hybridization between Apis mellifera mellifera and A.m. carnica in Czechoslovakia were electrophoretically examined at eighteen enzyme loci. The following five enzymes were found to exhibit polymorphism : malate dehydrogenase, esterase, malic enzyme, phosphoglucomutase and aconitase. The polymorphism for aconitase is reported here for the first time in the honey bee. Based on electrophoretic and morphometric data, many of the bees examined cannot be considered pure mellifera or carnica.