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Journal of General and Molecular Virology OPEN ACCESS Journal of General and Molecular Virology December 2018 ISSN 2141-6648 DOI: 10.5897/JGMV www.academicjournals.org About JGMV Journal of General and Molecular Virology (JGMV) is a peer reviewed journal. The journal is published per article and covers all areas of the subject such as: Isolation of chikungunya virus from non-human primates, Functional analysis of Lassa virus glycoprotein from a newly identified Lassa virus strain for possible use as vaccine using computational methods, as well as Molecular approaches towards analyzing the viruses infecting maize. JGMV welcomes the submission of manuscripts. Manuscripts should be submitted online via the Academic Journals Manuscript Management System. Indexing The Journal of General and Molecular Virology is indexed in Chemical Abstracts (CAS Source Index) and Dimensions Database Open Access Policy Open Access is a publication model that enables the dissemination of research articles to the global community without restriction through the internet. All articles published under open access can be accessed by anyone with internet connection. The Journal of General and Molecular Virology is an Open Access journal. Abstracts and full texts of all articles published in this journal are freely accessible to everyone immediately after publication without any form of restriction. Article License All articles published by Journal of General and Molecular Virology are licensed under the Creative Commons Attribution 4.0 International License. This permits anyone to copy, redistribute, remix, transmit and adapt the work provided the original work and source is appropriately cited. Citation should include the article DOI. The article license is displayed on the abstract page the following statement: This article is published under the terms of the Creative Commons Attribution License 4.0 Please refer to https://creativecommons.org/licenses/by/4.0/legalcode for details about Creative Commons Attribution License 4.0 Article Copyright When an article is published by in the Journal of General and Molecular Virology, the author(s) of the article retain the copyright of article. Author(s) may republish the article as part of a book or other materials. When reusing a published article, author(s) should; Cite the original source of the publication when reusing the article. i.e. cite that the article was originally published in the Journal of General and Molecular Virology. Include the article DOI Accept that the article remains published by the Journal of General and Molecular Virology (except in occasion of a retraction of the article) The article is licensed under the Creative Commons Attribution 4.0 International License. A copyright statement is stated in the abstract page of each article. The following statement is an example of a copyright statement on an abstract page. Copyright ©2016 Author(s) retains the copyright of this article. Self-Archiving Policy The Journal of General and Molecular Virology is a RoMEO green journal. This permits authors to archive any version of their article they find most suitable, including the published version on their institutional repository and any other suitable website. Please see http://www.sherpa.ac.uk/romeo/search.php?issn=1684-5315 Digital Archiving Policy The Journal of General and Molecular Virology is committed to the long-term preservation of its content. All articles published by the journal are preserved by Portico. In addition, the journal encourages authors to archive the published version of their articles on their institutional repositories and as well as other appropriate websites. https://www.portico.org/publishers/ajournals/ Metadata Harvesting The Journal of General and Molecular Virology encourages metadata harvesting of all its content. The journal fully supports and implement the OAI version 2.0, which comes in a standard XML format. See Harvesting Parameter Memberships and Standards Academic Journals strongly supports the Open Access initiative. Abstracts and full texts of all articles published by Academic Journals are freely accessible to everyone immediately after publication. All articles published by Academic Journals are licensed under the Creative Commons Attribution 4.0 International License (CC BY 4.0). This permits anyone to copy, redistribute, remix, transmit and adapt the work provided the original work and source is appropriately cited. Crossref is an association of scholarly publishers that developed Digital Object Identification (DOI) system for the unique identification published materials. Academic Journals is a member of Crossref and uses the DOI system. All articles published by Academic Journals are issued DOI. Similarity Check powered by iThenticate is an initiative started by CrossRef to help its members actively engage in efforts to prevent scholarly and professional plagiarism. Academic Journals is a member of Similarity Check. CrossRef Cited-by Linking (formerly Forward Linking) is a service that allows you to discover how your publications are being cited and to incorporate that information into your online publication platform. Academic Journals is a member of CrossRef Cited-by. Academic Journals is a member of the International Digital Publishing Forum (IDPF). The IDPF is the global trade and standards organization dedicated to the development and promotion of electronic publishing and content consumption. Contact Editorial Office: [email protected] Help Desk: [email protected] Website: http://www.academicjournals.org/journal/JGMV Submit manuscript online http://ms.academicjournals.org Academic Journals 73023 Victoria Island, Lagos, Nigeria ICEA Building, 17th Floor, Kenyatta Avenue, Nairobi, Kenya Editors Dr. Hossam M. Ashour Department of Microbiology and Immunology Faculty of Pharmacy Cairo University Egypt. Dr. Radhika Gopal Cell and Molecular Biology The Scripps Research Institute San Diego, CA USA. Table of Content Genetic diversity of rice yellow mottle virus from Niger Office and Selingue Development Rural Office in Mali Samaké Salimatou, Traoré Diakaridia, Goita Oumarou, Sarra Soungalo, Dao Sognan, Doumbia Bakaye and Amadou Hamadoun Babana Vol. 8(1), pp. 1-7, December, 2018 DOI: 10.5897/JGMV2018.0072 Article Number: 503733A59743 ISSN 2141-6648 Copyright © 2018 Author(s) retain the copyright of this article Journal of General and Molecular Virology http://www.academicjournals.org/JGMV Full Length Research Paper Genetic diversity of rice yellow mottle virus from Niger Office and Selingue Development Rural Office in Mali Samaké Salimatou1, Traoré Diakaridia1,3, Goita Oumarou2, Sarra Soungalo2, Dao Sognan1, Doumbia Bakaye1 and Amadou Hamadoun Babana1* 1Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Sciences and Techniques, University of Sciences, Techniques and Technologies, Bamako, BP E3206, Mali. 2Institute of Rural Economy, Bamako, BP E 438, Mali. 3National Center for Artificial Insemination, Bamako, BP E 5451, Mali. Received 10 October, 2018; Accepted 12 December, 2018 Virus is the most constraint of rice production in West Africa. Genetic diversity of rice yellow mottle virus (RYMV) was assessed in two rice growing areas of Mali. Forty-three (43) infected rice leaf samples were collected from five locations within the Niger Office and Selingue Development Rural Office rice’s growing areas. The infected samples were confirmed by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) using primers pair Prymv1 and Prymv2. A total of twenty-six RYMV isolates were obtained and classified into two genotypes A and B with Prymv1 and one genotype with Prymv2. Sequencing of the cDNA fragments with Prymv2 shared isolates into three distinct groups I, II and III. Most of the isolates were under Group I whereas isolates from the Selingue Development Rural Office area and Moussa-Were (Niger Office) area constituted respectively Groups II and III. This study provided baseline data for monitoring the genetic diversity of rice yellow mottle virus in the two rice-growing areas of Mali which could contribute to strategies development for the disease control. Key words: Genetic diversity, reverse transcriptase polymerase chain reaction (RT-PCR), rice yellow mottle virus (RYMV), sequencing, Mali. INTRODUCTION Yellow mottle disease of rice is caused by a virus that Research Sub-Station and Niger Office rice growing area belongs to the group sobemovirus (Hull and Fargette, in the Niono sector in Mali (Anonymous, 1992). The 2005) and is endemic to only the African continent (IRD, incidence of the disease varied from 64 to 100% in Niger 2014). This disease is more severe in irrigated rice- Office zone (WARDA, 2000) and 25 to 80% in Selingue growing areas located in sub-Sahara Africa and the most Development Rural Office depending on the date of damaging disease in rice cultivation (APCAM, 2004; IRD, infection and the susceptibility of rice cultivars (APCAM, 2014; Sere et al., 2013). It was first recorded on IET 2911 2004). Most rice cultivars are susceptible to this virus and BG 90-2 varieties in 1991 at Kogoni Agricultural (APCAM, 2004). Some varieties such as Gigante (Oryza *Corresponding author. E-mail: [email protected]. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License 2 J. Gen. Mol. Virol. sativa) and Tog series (Oryza glaberrima) showed natural Chain Reaction (RT-PCR) with two specific primer pairs PRymv1 resistance (Thottappilly and
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