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Table of Content Genetic diversity of rice yellow mottle virus from Niger Office and Selingue Development Rural Office in Mali Samaké Salimatou, Traoré Diakaridia, Goita Oumarou, Sarra Soungalo, Dao Sognan, Doumbia Bakaye and Amadou Hamadoun Babana Vol. 8(1), pp. 1-7, December, 2018 DOI: 10.5897/JGMV2018.0072 Article Number: 503733A59743 ISSN 2141-6648 Copyright © 2018 Author(s) retain the copyright of this article Journal of General and Molecular Virology http://www.academicjournals.org/JGMV Full Length Research Paper Genetic diversity of rice yellow mottle virus from Niger Office and Selingue Development Rural Office in Mali Samaké Salimatou1, Traoré Diakaridia1,3, Goita Oumarou2, Sarra Soungalo2, Dao Sognan1, Doumbia Bakaye1 and Amadou Hamadoun Babana1* 1Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Sciences and Techniques, University of Sciences, Techniques and Technologies, Bamako, BP E3206, Mali. 2Institute of Rural Economy, Bamako, BP E 438, Mali. 3National Center for Artificial Insemination, Bamako, BP E 5451, Mali. Received 10 October, 2018; Accepted 12 December, 2018 Virus is the most constraint of rice production in West Africa. Genetic diversity of rice yellow mottle virus (RYMV) was assessed in two rice growing areas of Mali. Forty-three (43) infected rice leaf samples were collected from five locations within the Niger Office and Selingue Development Rural Office rice’s growing areas. The infected samples were confirmed by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) using primers pair Prymv1 and Prymv2. A total of twenty-six RYMV isolates were obtained and classified into two genotypes A and B with Prymv1 and one genotype with Prymv2. Sequencing of the cDNA fragments with Prymv2 shared isolates into three distinct groups I, II and III. Most of the isolates were under Group I whereas isolates from the Selingue Development Rural Office area and Moussa-Were (Niger Office) area constituted respectively Groups II and III. This study provided baseline data for monitoring the genetic diversity of rice yellow mottle virus in the two rice-growing areas of Mali which could contribute to strategies development for the disease control. Key words: Genetic diversity, reverse transcriptase polymerase chain reaction (RT-PCR), rice yellow mottle virus (RYMV), sequencing, Mali. INTRODUCTION Yellow mottle disease of rice is caused by a virus that Research Sub-Station and Niger Office rice growing area belongs to the group sobemovirus (Hull and Fargette, in the Niono sector in Mali (Anonymous, 1992). The 2005) and is endemic to only the African continent (IRD, incidence of the disease varied from 64 to 100% in Niger 2014). This disease is more severe in irrigated rice- Office zone (WARDA, 2000) and 25 to 80% in Selingue growing areas located in sub-Sahara Africa and the most Development Rural Office depending on the date of damaging disease in rice cultivation (APCAM, 2004; IRD, infection and the susceptibility of rice cultivars (APCAM, 2014; Sere et al., 2013). It was first recorded on IET 2911 2004). Most rice cultivars are susceptible to this virus and BG 90-2 varieties in 1991 at Kogoni Agricultural (APCAM, 2004). Some varieties such as Gigante (Oryza *Corresponding author. E-mail: [email protected]. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License 2 J. Gen. Mol. Virol. sativa) and Tog series (Oryza glaberrima) showed natural Chain Reaction (RT-PCR) with two specific primer pairs PRymv1 resistance (Thottappilly and