Antiproliferative Effect of Angelica Archangelica Fruits Steinthor Sigurdssona,*, Helga M

Antiproliferative Effect of Angelica archangelica Fruits Steinthor Sigurdssona,*, Helga M. Ögmundsdottirb, and Sigmundur Gudbjarnasona a Science Institute, University of Iceland, Vatnsmyrarvegur 16, IS-101 Reykjavik, Iceland. Fax: +354 525 4886. E-mail: [email protected] b Molecular and Cell Biology Research Laboratory, Icelandic Cancer Society, Skogarhlid 8, IS-101 Reykjavik, Iceland * Author for correspondence and reprint requests Z. Naturforsch. 59c, 523Ð527 (2004); received December 21, 2003/February 9, 2004 The aim of this work was to study the antiproliferative effect of a tincture from fruits of Angelica archangelica and the active components using the human pancreas cancer cell line PANC-1 as a model. Significant dose-dependent antiproliferative activity was observed in µ the tincture with an EC50 value of 28.6 g/ml. Strong antiproliferative activity resulted from the two most abundant furanocoumarins in the tincture, imperatorin and xanthotoxin. The contribution of terpenes to this activity was insignificant. Imperatorin and xanthotoxin µ µ proved to be highly antiproliferative, with EC50 values of 2.7 g/ml and 3.7 g/ml, respectively, equivalent to 10 and 17 µm. The results indicate that furanocoumarins account for most of the antiproliferative activity of the tincture. Key words: Angelica archangelica, Xanthotoxin, Imperatorin Introduction their inhibiting effect on cytochrome P450, result- Angelica archangelica has been long and widely ing in drug-interactions (Guo et al., 2000; Koenigs used in folk medicine, and it is one of the most and Trager, 1998; Zhang et al., 2001). Imperatorin respected medicinal herbs in Nordic countries, has also been found to decrease chemically in- where it was cultivated during the Middle Ages, duced DNA adduct formation and may thus pos- and exported to other parts of Europe. The most sess chemopreventive activity by impairing the characteristic secondary metabolites of its fruits conversion of polycyclic hydrocarbons to carcino- are essential oils and furanocoumarins (Newall genic products (Kleiner et al., 2001). et al., 1996). Xanthotoxin and imperatorin have been shown Xanthotoxin (8-methoxypsoralen) and impera- to inhibit proliferation in HeLa cells by measuring torin (8-isopentenyloxypsoralen), shown in Fig. 1, their effect on colony formation, xanthotoxin be- are linear furanocoumarins occurring in a number ing more active than imperatorin (Gawron and of plants. Furanocoumarins have been extensively Glowniak, 1987). In another study, xanthotoxin studied and photosensitization very well docu- has been found to be cytotoxic to three cancer cell mented (Murray et al., 1982). Phototoxicity of xan- lines, whereas no activity could be demonstrated thotoxin is much higher than that of imperatorin for imperatorin (Saqib et al., 1990). In the same (Colombain et al., 2001). Light-independent activ- study lethality to brine shrimp larvae was demon- ity has also been the subject of numerous studies. strated for xanthotoxin whereas none was found Most recently, furanocoumarins were studied for in the case of imperatorin. In more recent studies antiproliferative activity of xanthotoxin against MK-1, HeLa, and B16F10 cell lines has been re- ported (Fujioka et al., 1999) and imperatorin was shown to inhibit proliferation of several cancer cell O O O O O O lines [human lung cancer (A549), melanin pigment O O a OMe b producing mouse melanoma (B16 melanoma 4A5), human T-cell leukemia (CCRF-HSB-2) and human gastric cancer, lymph node metastasized (TGBC11TKB)] whereas normal human cell lines Fig. 1. Structures of imperatorin (a) and xanthotoxin (b). [human umbilical vein endothelial cells (HUVE) 0939Ð5075/2004/0700Ð0523 $ 06.00 ” 2004 Verlag der Zeitschrift für Naturforschung, Tübingen · http://www.znaturforsch.com · D 524 S. Sigurdsson et al. · Antiproliferative Effect of Angelica archangelica Fruits and normal human foreskin keratinocytes (HFK)] from 40Ð60% MeOH in water, detection at 305 were less affected (Kawaii et al., 2001). Further- and 309 nm at room temperature, with a flow rate more, imperatorin has been shown to induce of 1.0 ml minÐ1. The tincture was diluted tenfold apoptosis of Hl-60 cells at micromolar concentra- before measurement, and its signal at 305 and tions (Pae et al., 2002). 309 nm was monitored and compared to standard The aim of this study was to evaluate the anti- curves with known amounts of xanthotoxin and proliferative activity of a tincture from A. archan- imperatorin. In the case of the other furanocou- gelica fruits, and to identify its active components. marins, the same relationship of signal to concentration was assumed as for xanthotoxin and impe- Material and Methods ratorin, correcting with the respective extinction coefficients at their maxima at about 300 nm (Lee Cancer cells and Soine, 1969). The pancreas-cancer cell line PANC-1 (Ameri- can Type Culture Collection, Rockville, MD) was Removal of essential oils or volatile compounds cultured in RPMI-1640 medium with 10% foetal from the tincture calf serum, 50 int. units mlÐ1 penicillin, 50 µgmlÐ1 streptomycin, 0.01 m N-(2-hydroxyethyl)piper- The essential oils present in the tincture were azine-NЈ-(2-ethanesulfonic acid)-buffer (HEPES) also examined separately. The tincture was slowly and 0.2 ml-glutamine (all from Gibco, Paisley, distilled, until 55% of the volume of the original UK). The cultures were incubated at 37 ∞Cin95% tincture had been evaporated, and the boiling temperature had reached 93 ∞C, removing the volatile humidity and 5% CO2. The cultures were pas- saged once a week by trypsinization using a 1:30 oil components and ethanol. The destillate was dilution of standard Gibco trypsin-EDTA solu- then diluted again with water to ensure that the tion. destillate contained the volatile oil compounds in the same concentration as originally present in Tincture the tincture. 60 g of dried fruits from A. archangelica, grow- Assessment of DNA synthesis ing in the southwest of Iceland, were ground. The resulting powder was then incubated in the dark The cells were trypsinized, counted and placed at room temperature in 45% ethanol, 10 ml for in 96-well plates at 104 cells per well. The test com- each gram of fruits. After six weeks the tincture pounds were dissolved, serially diluted before they was filtered and stored at room temperature. The were added at the start of the culture. The tincture weight of the dried residue of the tincture was and the volatile compounds from the tincture were 18.8 mg/ml. diluted in 45% ethanol (v/v), the isolated furanocoumarins in 60% (v/v) ethanol. The ethanol con- Furanocoumarin compounds centration in each culture, including the controls, was 3% (v/v). After 24 h of culture [3H]-thymidine Imperatorin was isolated by soxhlet-extraction was added at 37,000 Bq per well, and 4 h later the with n-hexane and isolated on a silica gel column, cells were washed and trypsinized, and harvested eluted with increasing amounts of diethyl ether in in a Skatron Cell Harvester (Skatron Instruments, hexane. It was recrystallized twice from a mixture Inc., Sterling, VA, USA) on to a glass fibre filter of n-hexane and diethyl ether and its purity was (Titertek, Huntsville, AL, USA). These were dried more than 95%. Xanthotoxin was purchased from and the radioactivity was counted in a liquid scin- Aldrich, MI, USA. tillation counter. Assessment of furanocoumarin in the tincture Results with HPLC Concentrations of imperatorin and xanthotoxin HP-1100 with a quaternary pump was used for in the tincture the mobile phase. The column was from Peco- sphere (Perkin-Elmer Labs, Norwalk, CT, USA) The most abundant furanocoumarins in the tinc- with a C18 stationary phase with a particle size of ture were imperatorin and xanthotoxin, 0.90 mg/ 3 µm, 33 ¥ 4.6 mm I.D. Gradient elution: 0Ð8 min ml and 0.32 mg/ml, respectively. Other detectable S. Sigurdsson et al. · Antiproliferative Effect of Angelica archangelica Fruits 525 furanocoumarins were identified as isoimperatorin 100 (0.29 mg/ml), isopimpinellin (0.17 mg/ml), oxy- 90 peucedanin (0.14 mg/ml) and bergapten (0.13 mg/ ml). The dry weight of the tincture was 18.8 mg/ 80 ml, so imperatorin accounted for almost 5% of its 70 dry weight and the total furanocoumarin-content was estimated as 1.96 mg/ml, or 10Ð11% of the 60 dry material of the tincture. 50 Inhibition of cell proliferation 40 Inhibition (%) The dose-dependent inhibition of cancer cell 30 proliferation by the tincture was measured in three separate experiments, each done in triplicate. The 20 tincture was diluted serially in 45% ethanol, four- 10 fold each time. The results are shown in Fig. 2. The Tincture Purified imperatorin Purified xanthotoxin highest concentration tested was 307 µg/ml, with 0 almost 100% inhibition. The inhibition decreased 0,1 1 10 100 continually with dilution, and at 16-fold dilution [µg/ml] (19 µg/ml) the inhibition was about 40%. The EC value was estimated as 28.6 µg/ml. Fig. 3. The dose-dependent inhibition of PANC-1 cell 50 proliferation by total furanocoumarins in the tincture, The fraction containing the essential oil or vola- assuming that these compounds are responsible for its tile compounds, diluted in the same way as the tinc- activity, compared with that of imperatorin and xantho- ture, had no measurable effect on cell proliferation. toxin. The furanocoumarins, imperatorin and xanthotoxin, were serially diluted, twofold in each step. The concentration-range was 1.6Ð26 and 2.3Ð 37 µg/ml for imperatorin and xanthotoxin, respectively. Both compounds inhibited cell-proliferation in a dose-dependent manner, as shown in Fig. 2. Imperatorin showed more activity, its EC50 value being estimated as 2.7 µg/ml and that of xanthotoxin as 3.7 µg/ml, or 10 and 17 µm, respectively. Fig. 3 compares the antiproliferative activity of the tincture, as a function of its furanocoumarin- content, with that of imperatorin and xanthotoxin.

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