Bull Mar Sci. 96(3):469–486. 2020 research paper https://doi.org/10.5343/bms.2018.0093 Feeding habitats of juvenile reef fishes in a tropical mangrove–seagrass continuum along a Malaysian shallow-water coastal lagoon 1 Institute of Oceanography Dung Quang Le 1 * and Environment, Universiti 1 Malaysia Terengganu, 21030 Siau Yin Fui 2 Kuala Nerus, Terengganu, Kentaro Tanaka Malaysia Suhaimi Suratman 1 2 Atmosphere and Ocean Yuji Sano 2 Research Institute, The 2 University of Tokyo, 5-1-5, Kotaro Shirai Kashiwanoha, Kashiwa-shi, Chiba 277-8564 Japan * Corresponding author email: ABSTRACT.—We conducted stable isotope (δ13C and <
[email protected]>, <le. δ15N) and gut content analyses to understand habitat use
[email protected]> of juvenile reef fishes (Lethrinus lentjan, Lutjanus russellii, and Epinephelus coioides), particularly the influence of spatial habitat structure along seagrass–mangrove continua. Sampling was conducted in Setiu Lagoon, located in the southern waters of the South China Sea (Malaysia). Gut content analysis indicated that the focal fishes preyed mainly on benthic invertebrates and other smaller fishes; these were used as potential prey items for isotopic tracers to track fish habitat use. Stable isotope analyses highlighted that the juvenile reef fishes were highly associated with seagrass beds as feeding grounds; however, site-specific analyses suggest differences in fish feeding habitats. The juvenile fishes, particularly large juveniles, preyed in both mangrove and seagrass areas from the central lagoon, whereas all juveniles showed preferential foraging within seagrass meadows in the nearby lagoon mouth. Furthermore, the mean stable isotope values of muscle tissue differed among fish size classes, indicating their ontogenetic habitat/diet shifts, especially for L.