Integrin Beta 7 Antibody (F44498)

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Integrin beta 7 Antibody (F44498) Catalog No. Formulation Size F44498-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml F44498-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml Bulk quote request Availability 1-3 business days Species Reactivity Human Format Antigen affinity purified Clonality Polyclonal (rabbit origin) Isotype Rabbit Ig Purity Antigen affinity UniProt P26010 Applications Western blot : 1:1000 Limitations This Integrin beta 7 antibody is available for research use only. Integrin beta 7 antibody western blot analysis in WiDr lysate. Description Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin. Application Notes Titration of the Integrin beta 7 antibody may be required due to differences in protocols and secondary/substrate sensitivity. Immunogen A portion of amino acids 737-765 from the human protein was used as the immunogen for this Integrin beta 7 antibody. Storage Aliquot the Integrin beta 7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. Ordering: Phone:858.663.9055 | Fax:1.267.821.0800 | Email:[email protected] Copyright © NSJ Bioreagents. All rights reserved.

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Supplementary Table 1: Adhesion Genes Data Set
Supplementary Table 1: Adhesion genes data set PROBE Entrez Gene ID Celera Gene ID Gene_Symbol Gene_Name 160832 1 hCG201364.3 A1BG alpha-1-B glycoprotein 223658 1 hCG201364.3 A1BG alpha-1-B glycoprotein 212988 102 hCG40040.3 ADAM10 ADAM metallopeptidase domain 10 133411 4185 hCG28232.2 ADAM11 ADAM metallopeptidase domain 11 110695 8038 hCG40937.4 ADAM12 ADAM metallopeptidase domain 12 (meltrin alpha) 195222 8038 hCG40937.4 ADAM12 ADAM metallopeptidase domain 12 (meltrin alpha) 165344 8751 hCG20021.3 ADAM15 ADAM metallopeptidase domain 15 (metargidin) 189065 6868 null ADAM17 ADAM metallopeptidase domain 17 (tumor necrosis factor, alpha, converting enzyme) 108119 8728 hCG15398.4 ADAM19 ADAM metallopeptidase domain 19 (meltrin beta) 117763 8748 hCG20675.3 ADAM20 ADAM metallopeptidase domain 20 126448 8747 hCG1785634.2 ADAM21 ADAM metallopeptidase domain 21 208981 8747 hCG1785634.2|hCG2042897 ADAM21 ADAM metallopeptidase domain 21 180903 53616 hCG17212.4 ADAM22 ADAM metallopeptidase domain 22 177272 8745 hCG1811623.1 ADAM23 ADAM metallopeptidase domain 23 102384 10863 hCG1818505.1 ADAM28 ADAM metallopeptidase domain 28 119968 11086 hCG1786734.2 ADAM29 ADAM metallopeptidase domain 29 205542 11085 hCG1997196.1 ADAM30 ADAM metallopeptidase domain 30 148417 80332 hCG39255.4 ADAM33 ADAM metallopeptidase domain 33 140492 8756 hCG1789002.2 ADAM7 ADAM metallopeptidase domain 7 122603 101 hCG1816947.1 ADAM8 ADAM metallopeptidase domain 8 183965 8754 hCG1996391 ADAM9 ADAM metallopeptidase domain 9 (meltrin gamma) 129974 27299 hCG15447.3 ADAMDEC1 ADAM-like,
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Integrin Beta 7 Polyclonal Antibody Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP54281
10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Integrin beta 7 Polyclonal Antibody Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP54281 Specification Integrin beta 7 Polyclonal Antibody - Product Information Application IHC-P Primary Accession P26011 Reactivity Rat Host Rabbit Clonality Polyclonal Calculated MW 87411 Integrin beta 7 Polyclonal Antibody - Additional Information Gene ID 16421 Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and Other Names paraffin-embedded; Integrin beta-7, Integrin beta-P, M290 IEL Antigen retrieval: citrate buffer ( 0.01M, pH antigen, Itgb7 6.0 ), Boiling bathing for 15min; Block Format endogenous peroxidase by 3% Hydrogen 0.01M TBS(pH7.4) with 1% BSA, 0.09% peroxide for 30min; Blocking buffer (normal (W/V) sodium azide and 50% Glyce goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Integrin beta 7 Polyclonal Storage Antibody, Unconjugated(bs-1051R) 1:200, Store at -20 ℃ for one year. Avoid repeated overnight at 4°C, followed by conjugation to freeze/thaw cycles. When reconstituted in the secondary antibody(SP-0023) and sterile pH 7.4 0.01M PBS or diluent of DAB(C-0010) staining antibody the antibody is stable for at least two weeks at 2-4 ℃. Integrin beta 7 Polyclonal Antibody - Protein Information Name Itgb7 Function Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is involved in adhesive interactions of leukocytes. It is a receptor for fibronectin and recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1 and VCAM1.
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Cd49d Antibody [R1-2] Cat
CD49d Antibody [R1-2] Cat. No.: 76-809 CD49d Antibody [R1-2] Specifications HOST SPECIES: Rat SPECIES REACTIVITY: Mouse TESTED APPLICATIONS: Flow, Func The R1-2 monoclonal antibody specifically reacts with mouse CD49d (integrin alpha 4), SPECIFICITY: which forms the VLA-4 complex with CD29 (integrin beta 1). Properties The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin PURIFICATION: level is determined by LAL test to be less than 0.01 EU/µg of the protein. CLONALITY: Monoclonal ISOTYPE: Rat IgG2b, kappa CONJUGATE: Unconjugated PHYSICAL STATE: liquid BUFFER: Phosphate-buffered aqueous solution, ph7.2. CONCENTRATION: batch dependent STORAGE CONDITIONS: The product should be stored undiluted at 4˚C . Do not freeze. September 27, 2021 1 https://www.prosci-inc.com/cd49d-antibody-r1-2-76-809.html Additional Info OFFICIAL SYMBOL: Itga4 ALTERNATE NAMES: CD49D, Itga4B, Itga4 GENE ID: 16401 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References The R1-2 monoclonal antibody specifically reacts with mouse CD49d (integrin alpha 4), which forms the VLA-4 complex with CD29 (integrin beta 1). VLA-4 is expressed on BACKGROUND: thymocytes, monocytes, and a subset of peripheral lymphocytes. It is the receptor for fibronectin and CD106 (VCAM-1). CD49d also forms a receptor for mucosal vascular addressin (MAdCAM-1) with integrin beta 7. 1) Deckert, M., Kubar, J., Zoccola, D., Bernard‐Pomier, G., Angelisova, P., Horejsi, V., REFERENCES: Bernard, A. (1992). CD59 molecule: a second ligand for CD2 in T cell adhesion.European journal of immunology,22(11), 2943-2947. 2) Korty, P.
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Supp Table 6.Pdf
Supplementary Table 6. Processes associated to the 2037 SCL candidate target genes ID Symbol Entrez Gene Name Process NM_178114 AMIGO2 adhesion molecule with Ig-like domain 2 adhesion NM_033474 ARVCF armadillo repeat gene deletes in velocardiofacial syndrome adhesion NM_027060 BTBD9 BTB (POZ) domain containing 9 adhesion NM_001039149 CD226 CD226 molecule adhesion NM_010581 CD47 CD47 molecule adhesion NM_023370 CDH23 cadherin-like 23 adhesion NM_207298 CERCAM cerebral endothelial cell adhesion molecule adhesion NM_021719 CLDN15 claudin 15 adhesion NM_009902 CLDN3 claudin 3 adhesion NM_008779 CNTN3 contactin 3 (plasmacytoma associated) adhesion NM_015734 COL5A1 collagen, type V, alpha 1 adhesion NM_007803 CTTN cortactin adhesion NM_009142 CX3CL1 chemokine (C-X3-C motif) ligand 1 adhesion NM_031174 DSCAM Down syndrome cell adhesion molecule adhesion NM_145158 EMILIN2 elastin microfibril interfacer 2 adhesion NM_001081286 FAT1 FAT tumor suppressor homolog 1 (Drosophila) adhesion NM_001080814 FAT3 FAT tumor suppressor homolog 3 (Drosophila) adhesion NM_153795 FERMT3 fermitin family homolog 3 (Drosophila) adhesion NM_010494 ICAM2 intercellular adhesion molecule 2 adhesion NM_023892 ICAM4 (includes EG:3386) intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)adhesion NM_001001979 MEGF10 multiple EGF-like-domains 10 adhesion NM_172522 MEGF11 multiple EGF-like-domains 11 adhesion NM_010739 MUC13 mucin 13, cell surface associated adhesion NM_013610 NINJ1 ninjurin 1 adhesion NM_016718 NINJ2 ninjurin 2 adhesion NM_172932 NLGN3 neuroligin
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Cell Adhesion Molecules in Normal Skin and Melanoma
biomolecules Review Cell Adhesion Molecules in Normal Skin and Melanoma Cian D’Arcy and Christina Kiel * Systems Biology Ireland & UCD Charles Institute of Dermatology, School of Medicine, University College Dublin, D04 V1W8 Dublin, Ireland; [email protected] * Correspondence: [email protected]; Tel.: +353-1-716-6344 Abstract: Cell adhesion molecules (CAMs) of the cadherin, integrin, immunoglobulin, and selectin protein families are indispensable for the formation and maintenance of multicellular tissues, espe- cially epithelia. In the epidermis, they are involved in cell–cell contacts and in cellular interactions with the extracellular matrix (ECM), thereby contributing to the structural integrity and barrier for- mation of the skin. Bulk and single cell RNA sequencing data show that >170 CAMs are expressed in the healthy human skin, with high expression levels in melanocytes, keratinocytes, endothelial, and smooth muscle cells. Alterations in expression levels of CAMs are involved in melanoma propagation, interaction with the microenvironment, and metastasis. Recent mechanistic analyses together with protein and gene expression data provide a better picture of the role of CAMs in the context of skin physiology and melanoma. Here, we review progress in the field and discuss molecular mechanisms in light of gene expression profiles, including recent single cell RNA expression information. We highlight key adhesion molecules in melanoma, which can guide the identification of pathways and Citation: D’Arcy, C.; Kiel, C. Cell strategies for novel anti-melanoma therapies. Adhesion Molecules in Normal Skin and Melanoma. Biomolecules 2021, 11, Keywords: cadherins; GTEx consortium; Human Protein Atlas; integrins; melanocytes; single cell 1213. https://doi.org/10.3390/ RNA sequencing; selectins; tumour microenvironment biom11081213 Academic Editor: Sang-Han Lee 1.
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Anti-Human/Mouse Integrin beta 7 PE Catalog Number: 12-5867 Also Known As:Integrin b7 ITGB7 RUO: For Research Use Only. Not for use in diagnostic procedures. Staining of normal human peripheral blood cells with Rat IgG2a K Isotype Control PE (cat. 12-4321) (open histogram) or Anti- Human/Mouse Integrin beta 7 PE (filled histogram). Cells in the lymphocyte gate were used for analysis. Product Information Contents: Anti-Human/Mouse Integrin beta 7 PE Formulation: aqueous buffer, 0.09% sodium azide, may Catalog Number: 12-5867 contain carrier protein/stabilizer Clone: FIB504 Temperature Limitation: Store at 2-8°C. Do not freeze. Concentration: 5 uL (0.125 ug)/test Light sensitive material. Host/Isotype: Rat IgG2a, kappa Batch Code: Refer to Vial Use By: Refer to Vial Caution, contains Azide Description The FIB504 monoclonal antibody reacts with human and mouse integrin beta 7. Integrin beta 7 is a 130 kD glycoprotein which associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Main ligands for integrin alpha 4 beta 7 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the main ligand of integrin alpha E beta 7 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response. The FIB504 antibody has been reported to have blocking activity.
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STRUCTURAL INVESTIGATION of the COMPLEX of FILAMIN a REPEAT 21 with INTEGRIN Αiib & Β3 CYTOPLASMIC TAILS
STRUCTURAL INVESTIGATION OF THE COMPLEX OF FILAMIN A REPEAT 21 WITH INTEGRIN αIIb & β3 CYTOPLASMIC TAILS —A POTENTIAL “TRANSMISSION” TO REGULATE CELL MIGRATION JIANMIN LIU Bachelor of Science in Chemical Engineering Changchu University of Technology July, 1999 Master of Science in Chemistry Changchu University of Technology July, 2002 submitted in partial fulfillment of requirements for the degree DOCTOR OF PHILOSOPHY IN CLINICAL-BIOANALYTICAL CHEMISTRY at the CLEVELAND STATE UNIVERSITY August, 2009 This dissertation has been approved for the Department of CHEMISTRY and the College of Graduate Studies by Dissertation Chairman, Edward Plow Ph.D. Department & Date Jun Qin Ph.D. Department & Date Yan Xu Ph.D. Department & Date John Masnovi Ph.D. Department & Date Aimin Zhou Ph.D. Department & Date STRUCTURAL INVESTIGATION OF THE COMPLEX OF FILAMIN A REPEAT 21 WITH INTEGRIN αIIb & β3 CYTOPLASMIC TAILS —A POTENTIAL “TRANSMISSION” TO REGULATE CELL MIGRATION JIANMIN LIU ABSTRACT Cell functions in multi-cellular organisms are strongly depend on the dynamic cooperation between cell adhesion and cytoskeleton reorganization. Integrins, the major cell adhesion receptors, bind to extracellular matrix (ECM) and soluble ligands on the cell surface and link to the actin cytoskeleton inside the cell membrane. In this manner, integrins integrate cell adhesion and cytoskeleton reorganization by acting as a mechanical force transducer and a biochemical signaling hub (Zamir and Geiger 2001). Consequently, integrins are vital for development, immune responses, leukocyte traffic and hemostasis, and a variety of other cellular and physiological processes. Integrins are also are the focal point of many human diseases, including genetic, autoimmune, cardiovascular and others. In terms of the cell-ECM adhesion, integrins can exist in two major states, active, where it binds to appropriate extracellular ligands, and inactive, where it disassociates from extracellular ligands.
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Journal name: OncoTargets and Therapy Article Designation: Original Research Year: 2016 Volume: 9 OncoTargets and Therapy Dovepress Running head verso: Zheng et al Running head recto: ITGA5 and ITGB1 are prognostic in NSCLC open access to scientific and medical research DOI: http://dx.doi.org/10.2147/OTT.S91796 Open Access Full Text Article ORIGINAL RESEARCH Integrin and gene network analysis reveals that ITGA5 and ITGB1 are prognostic in non-small-cell lung cancer Weiqi Zheng Background: Integrin expression has been identified as a prognostic factor in non-small-cell Caihui Jiang lung cancer (NSCLC). This study was aimed at determining the predictive ability of integrins Ruifeng Li and associated genes identified within the molecular network. Patients and methods: A total of 959 patients with NSCLC from The Cancer Genome Atlas Department of Radiation Oncology, Guangqian Hospital, Quanzhou, Fujian, cohorts were enrolled in this study. The expression profile of integrins and related genes were People’s Republic of China obtained from The Cancer Genome Atlas RNAseq database. Clinicopathological characteristics, including age, sex, smoking history, stage, histological subtype, neoadjuvant therapy, radiation therapy, and overall survival (OS), were collected. Cox proportional hazards regression models as well as Kaplan–Meier curves were used to assess the relative factors. Results: In the univariate Cox regression model, ITGA1, ITGA5, ITGA6, ITGB1, ITGB4, and ITGA11 were predictive of NSCLC prognosis. After adjusting for clinical factors, ITGA5 (odds ratio =1.17, 95% confidence interval: 1.05–1.31) andITGB1 (odds ratio =1.31, 95% confidence interval: 1.10–1.55) remained statistically significant. In the gene cluster network analysis, PLAUR, ILK, SPP1, PXN, and CD9, all associated with ITGA5 and ITGB1, were identified as independent predictive factors of OS in NSCLC.
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The Concise Guide to Pharmacology 2019/20: Catalytic Receptors
Alexander, S. P. H., Fabbro, D., Kelly, E., Mathie, A., Peters, J. A., Veale, E. L., Armstrong, J. F., Faccenda, E., Harding, S. D., Pawson, A. J., Sharman, J. L., Southan, C., Davies, J. A., & CGTP Collaborators (2019). The Concise Guide to Pharmacology 2019/20: Catalytic receptors. British Journal of Pharmacology, 176(S1), S247- S296. https://doi.org/10.1111/bph.14751 Publisher's PDF, also known as Version of record License (if available): CC BY Link to published version (if available): 10.1111/bph.14751 Link to publication record in Explore Bristol Research PDF-document This is the final published version of the article (version of record). It first appeared online via Wiley at https://bpspubs.onlinelibrary.wiley.com/doi/full/10.1111/bph.14751. Please refer to any applicable terms of use of the publisher. University of Bristol - Explore Bristol Research General rights This document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/red/research-policy/pure/user-guides/ebr-terms/ S.P.H. Alexander et al. The Concise Guide to PHARMACOLOGY 2019/20: Catalytic receptors. British Journal of Pharmacology (2019) 176, S247–S296 THE CONCISE GUIDE TO PHARMACOLOGY 2019/20: Catalytic receptors Stephen PH Alexander1 , Doriano Fabbro2 , Eamonn Kelly3, Alistair Mathie4 ,JohnAPeters5 , Emma L Veale4 , Jane F Armstrong6 , Elena Faccenda6 ,SimonDHarding6 ,AdamJPawson6 , Joanna L Sharman6 , Christopher Southan6 , Jamie A Davies6
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Integrin Beta 7 (ITGB7) (NM 000889) Human Tagged ORF Clone Product Data
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Role of Integrin A4я7/A4яp in Lymphocyte Adherence To
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