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Genome-Wide Association Meta-Analysis Using a Recessive Model Illuminates Genetic Architecture of Type 2 Diabetes Mark J

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Genome-Wide Association Meta-Analysis Using a Recessive Model Illuminates Genetic Architecture of Type 2 Diabetes Mark J medRxiv preprint doi: https://doi.org/10.1101/2021.07.08.21258700; this version posted July 8, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license . Genome-Wide Association Meta-Analysis Using a Recessive Model Illuminates Genetic Architecture of Type 2 Diabetes Mark J. O’Connor 1, 2, 3, 4, 5, Alicia Huerta-Chagoya 6, Paula Cortés-Sánchez 7, Silvía Bonàs-Guarch 7, Joanne B. Cole 4, 5, 8, 9, David Torrents 7, 10, Kumar Veerapen 8, 11, 12, Niels Grarup 13, Mitja Kurki 8, 11, 12, Carsten F. Rundsten 13, Oluf Pedersen 13, Ivan Brandslund 14, 15, Allan Linneberg 16, 17, Torben Hansen 13, Aaron Leong 1, 2, 3, 4, 5, 8, 18,#, Jose C. Florez 1, 2, 3, 4, 5, 8,#, Josep M. Mercader 3, 4, 5, 8,# 1. Department of Medicine, Massachusetts General Hospital, Boston, MA, USA. 2. Endocrine Division, Massachusetts General Hospital, Boston, MA, USA. 3. Diabetes Unit, Massachusetts General Hospital, Boston, MA, USA. 4. Center for Genomic Medicine, Massachusetts General Hospital, Boston, MA, USA. 5. Programs in Metabolism and Medical and Population Genetics, Broad Institute of Harvard and MIT, Cambridge, MA, USA. 6. Consejo Nacional de Ciencia y Tecnología (CONACYT), Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico. 7. Barcelona Supercomputing Center (BSC), Barcelona, Spain. 8. Department of Medicine, Harvard Medical School, Boston, MA, USA. 9. Center for Basic and Translations Obesity Research, Boston Children’s Hospital, Boston, MA, USA. 10. Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain. 11. Stanley Center for Psychiatric Genetics, Broad Institute of Harvard and MIT, Cambridge, MA, USA. 12. Analytic and Translational Genetics Unit, Massachusetts General Hospital, Boston, MA, USA. 13. Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2100, Copenhagen, Denmark. 14. Department of Clinical Biochemistry, Lillebaelt Hospital, Vejle, Denmark. 15. Institute of Regional Health Research, University of Southern Denmark, Odense, Denmark. 16. Center for Clinical Research and Prevention, Bispebjerg and Frederiksberg Hospital, Copenhagen, Denmark. 17. Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. 18. Division of General Internal Medicine, Massachusetts General Hospital, Boston, MA, USA. # These authors jointly directed this work. Correspondence: Josep M. Mercader, Ph.D. The Broad Institute of Harvard and MIT 75 Ames Street, Cambridge, MA 02142. [email protected] Phone: 617-714-7000 NOTE: This preprint reports new research that has not been certified by peer review and should not be used to guide clinical practice. medRxiv preprint doi: https://doi.org/10.1101/2021.07.08.21258700; this version posted July 8, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license . ABSTRACT Objective: Most genome-wide association studies (GWAS) of complex traits are performed using models with additive allelic effects. Hundreds of loci associated with type 2 diabetes have been identified using this approach. Additive models, however, can miss loci with recessive effects, thereby leaving potentially important genes undiscovered. Research Design and Methods: We conducted the largest GWAS meta-analysis using a recessive model for type 2 diabetes. Our discovery sample included 33,139 cases and 279,507 controls from seven European-ancestry cohorts including the UK Biobank. We then used two additional cohorts, FinnGen and a Danish cohort, for replication. For the most significant recessive signal, we conducted a phenome-wide association study across hundreds of traits to make inferences about the pathophysiology underlying the increased risk seen in homozygous carriers. Results: We identified 51 loci associated with type 2 diabetes, including five variants with recessive effects undetected by prior additive analyses. Two of the five had minor allele frequency less than 5% and were each associated with more than doubled risk. We replicated three of the variants, including one of the low-frequency variants, rs115018790, which had an odds ratio in homozygous carriers of 2.56 (95% CI 2.05- 3.19, P=110-16) and a stronger effect in men than in women (interaction P=710-7). Colocalization analysis linked this signal to reduced expression of the nearby PELO gene, and the signal was associated with multiple diabetes-related traits, with medRxiv preprint doi: https://doi.org/10.1101/2021.07.08.21258700; this version posted July 8, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license . homozygous carriers showing a 10% decrease in LDL and a 20% increase in triglycerides. Conclusions: Our results demonstrate that recessive models, when compared to GWAS using the additive approach, can identify novel loci, including large-effect variants with pathophysiological consequences relevant to type 2 diabetes. medRxiv preprint doi: https://doi.org/10.1101/2021.07.08.21258700; this version posted July 8, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license . INTRODUCTION Type 2 diabetes affects nearly 1 in 12 adults globally (1), but its genetic architecture is still not fully understood. Over the last decade, large genome-wide association studies (GWAS) have used additive models to identify hundreds of associated loci (2-4). Additive models are most powerful when the effect of two copies of a risk allele is twice that of one copy. This model is computationally simple and statistically powerful, but it does not always match the pattern of inheritance of Mendelian disorders, including monogenic forms of diabetes, which can be transmitted in a dominant or recessive fashion (5). Variants with recessive effects, particularly low-frequency variants, can go undetected by additive models (6), suggesting that non-additive models have the potential to generate new biological insights. To date, a handful of studies have used recessive models to identify genetic associations with type 2 diabetes, but these have been limited by small sample sizes (6-8). Nevertheless, some promising findings have emerged. In a Greenlandic population, homozygous carriers of two copies of a nonsense mutation in the TBC1D4 gene, which facilitates glucose transfer into skeletal muscle in the setting of insulin stimulation, were found to have a tenfold increase in diabetes risk compared to other individuals in the same population (7). Hyperglycemia due to this variant occurs postprandially, so the diagnosis of type 2 diabetes in homozygous carriers often requires an oral glucose tolerance test, creating an opportunity for precision medicine (9). More recently, members of our group conducted GWAS with non-additive models for several age-related diseases (10) and identified multiple new loci, including one rare variant (rs77704739) associated medRxiv preprint doi: https://doi.org/10.1101/2021.07.08.21258700; this version posted July 8, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license . with type 2 diabetes. This variant was also associated with reduced expression of the PELO gene, whose connection to diabetes is not well understood. We have conducted the largest GWAS meta-analysis using a recessive model reported to date for type 2 diabetes. Over the last few years, GWAS sample sizes have grown exponentially (11), and reference panels for imputation have improved, making it easier to ascertain low-frequency variants accurately (12). To take advantage of these developments, we combined data from seven discovery cohorts and two replication cohorts to conduct the largest recessive-model GWAS yet reported for type 2 diabetes or any other disease. We identified and replicated multiple variants missed by larger additive studies, confirmed and fine-mapped the association near PELO, and conducted a phenome-wide association analysis to identify other impacted traits to better understand the pathophysiology underlying this novel association. RESEARCH DESIGN AND METHODS Study Population and Outcome Definition We used data from multiple European-ancestry cohorts (Supplemental Table 1) including the UK Biobank (13), five cohorts known collectively as 70K for T2D (4), and the Mass General Brigham (MGB) Biobank (14). The UK Biobank is a sample of approximately half a million people recruited in the United Kingdom between the ages of 40 and 69 years. The 70K for T2D cohort consists of five studies with publicly available data, and the MGB Biobank consists of approximately 50,000 people recruited within a hospital system in the medRxiv preprint doi: https://doi.org/10.1101/2021.07.08.21258700; this version posted July 8, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license . United States. We only considered individuals whose family relatedness was lower than that of third-degree relatives. Definitions of type 2 diabetes varied according to cohort. In the UK Biobank, for example, we used a validated algorithm designed specifically to identify cases of diabetes in that cohort (15).
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