DOCSLIB.ORG

Impact of Bioactive Molecules Secreted by Lactobacillus Acidophilus La-5 on Clostridium Difficile Virulence Factors

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Impact of Bioactive Molecules Secreted by Lactobacillus Acidophilus La-5 on Clostridium Difficile Virulence Factors IMPACT OF BIOACTIVE MOLECULES SECRETED BY LACTOBACILLUS ACIDOPHILUS LA-5 ON CLOSTRIDIUM DIFFICILE VIRULENCE FACTORS by Afsaneh Najarian A Thesis Presented to The University of Guelph In partial fulfilment of requirements for the degree of Doctor of Philosophy in Food Science Guelph, Ontario, Canada ©Afsaneh Najarian, November 2017 ABSTRACT IMPACT OF BIOACTIVE MOLECULES SECRETED BY LACTOBACILLUS ACIDOPHILUS LA-5 ON CLOSTRIDIUM DIFFICILE VIRULENCE FACTORS Afsaneh Najarian Advisors: University of Guelph, 2017 Dr. Mansel W. Griffiths Dr. Shayan Sharif Clostridium difficile is a leading pathogen of hospital-associated diseases including infectious diarrhea and pseudomembranous colitis. Increasing frequency and severity of diseases besides inconsistent results of antibiotic therapy, demand alternative therapeutic candidates using a different strategy to control pathogenicity of C. difficile. Unlike antibiotics, antivirulence compounds control bacterial infection without killing them or developing antibiotic-resistant. Reducing essential virulence factors in C. difficile including toxin production, adherence, and biofilm formation could substantially minimize its pathogenicity and lead to a faster recovery from the disease. This study aimed to investigate antivirulence effects of La-5 bioactive molecules produced by Lactobacillus acidophilus La-5 namely proteobiotics on the pathogenicity of C. difficile. Three clinically important strains of C. difficile (ribotypes 027, 078, and 001) were incubated with or without reconstituted La-5 cell-free supernatant (CFS). The C. difficile culture filtrates were collected for the assessment of quorum sensing, and expression of several virulence genes performing reverse transcription q-PCR. C. difficile attachment and cytotoxicity in human epithelial cells were monitored in vitro using human epithelial cells HT-29 and Caco-2 monolayers. Additionally, L. acidophilus La-5 was incubated in a medium containing whey protein, and inhibitory effects of its CFS were tested performing quantitative biofilm assay then visualized in scanning electron microscopy (SEM). Bioactive molecules caused a significant down-regulation in expression of some of the virulence genes mainly two essential toxin genes tcdA and tcdB and adhesion gene Cwp84 in all C. difficile strains. A substantial down-regulation in luxS expression was observed in ribotype 027 and 078, and 001 (P<0.05). La-5 bioactive molecules also reduced cytotoxicity and cytopathic effect of C. difficile culture filtrate on HT-29 and Caco-2 monolayers significantly (P < 0.0.5). Furthermore, pre-incubation of cell monolayers and La-5 CFS reduced attachment of the C. difficile bacterial cells on both cell monolayers. The biofilm formation and population of C. difficile cells encased in biofilms were significantly decreased within the treated cultures (P < 0.0001). SEM analysis also confirmed quantitative results showing a sufficient reduction in biofilm formation. Our study suggests that La-5 bioactive molecules provide a supportive adjunct therapy for C. difficile infection, prevent relapses, and improve disease outcome. ACKNOWLEDGMENTS First and foremost, I would like to thank Professor Mansel Griffiths sincerely. Thank you for providing me the opportunity to conduct this research under your supervision. Your expert, knowledge, and endless support encouraged me throughout my Ph.D. program. You let me challenge myself and taught me to think critically inside and outside the box. My great appreciation goes to Dr. Shayan Sharif. Without your encouragement, support and guidance this thesis would not have been completed. I am extremely grateful to you for standing by me through the ups and downs I faced during my study. I am thankful to you for being supportive and for having faith in me. Exceptional thanks to Dr. Milena Corredig. Thank you for your help and support and for being one of the great members of my advisory committee. Your expert advice and your friendship throughout my research, and trusting in my ability gave me the strength to move forward with my program. I would like to express my sincere thanks to Dr. Mitra Amiri for being my great mentor. Your advice, vast knowledge, and friendship was great inspiration for me. Especial thanks to Dr. Alexandra (Sandy) Smith for microscopy training and assisting me with the sample preparation for scanning electron microscopy. A great thank you to Dr. Scott Weese for his guidance and expert advice, and to Joyce Rousseau in Ontario Veterinary College for providing me the C. difficile strains. I would like to thank Dr. Rocio Morales for her supervision and guidance. Also, I acknowledge all the past and present students and researchers of Canadian Research Institute for Food Safety (CRIFS) for sharing ideas, excellent cooperation and making our laboratory a lively and friendly workplace. I appreciate staff and students at “Dairy Lab” in Food Science Department for welcoming attitude and for supporting me during my work there and for their excellent cooperation. I am deeply thankful to the faculty and staff in the Food Science Department for their excellent support and to the University of Guelph and financial sources that supported this project to be completed. I would like to express my most profound gratitude to my husband Mohsen and my two sons Mohammad and Matin for their unconditional love, understanding, help, and sacrifices they made for me throughout my academic journey. My sincere thanks go to my late father, and my two late brothers for inspiring me to start this path, and my huge thanks go to my beloved mother and my dear sister and brothers for sharing my problems and supporting me during our losses. Last but not least a big thanks to all of my friends for cheering me up and supporting me by having me in their thoughts and prayers. iv TABLE OF CONTENTS ABSTRACT ................................................................................................................................................ II ACKNOWLEDGMENTS ........................................................................................................................ IV TABLE OF CONTENTS .......................................................................................................................... V LIST OF ABREVIATIONS ...................................................................................................................... X LIST OF FIGURES .............................................................................................................................. XIII LIST OF TABLES ................................................................................................................................. XIV CHAPTER 1 ................................................................................................................................................ 1 GENERAL INTRODUCTION AND THESIS OBJECTIVES ............................................................... 1 CHAPTER 2 ................................................................................................................................................ 6 LITERATURE REVIEW .......................................................................................................................... 6 2. CLOSTRIDIA ......................................................................................................................................... 6 2.1 CLOSTRIDIUM DIFFICILE............................................................................................................ 7 2.1.1 Clostridium difficile Microbiology ................................................................................................. 7 2.1.2 C. difficile Infection (CDI) and C. difficile associated diseases (CDAD) ................................... 8 2.1.3 Epidemiology and Variation in Virulence Factors of C. difficile Strains: Hypervirulent Strains .................................................................................................................................................... 10 2.1.4 Disease Transmission ................................................................................................................... 12 2.1.5 History of Clostridium difficile infectious disease ...................................................................... 13 2.2 CLOSTRIDIUM DIFFICILE VIRULENCE FACTORS ............................................................. 14 2.2.1 Non-toxigenic Virulence Factors: Colonization and Adherence.............................................. 14 2.2.2 Toxigenic Virulence Factors and Mechanism of Action ........................................................... 16 2.2.3 Binary Toxins ............................................................................................................................... 22 2.3 C. DIFFICILE OTHER VIRULENCE FACTORS ...................................................................... 23 2.3.1 C. difficile Sporulation and Germination ................................................................................... 23 v 2.3.2 C. difficile Quorum Sensing......................................................................................................... 25 2.3.3 C. difficile Motility ....................................................................................................................... 28 2.3.4 C. difficile Biofilm Formation ..................................................................................................... 29 2.4 CDI
Load more

Recommended publications
  • Integrating Conjugative Elements of the SXT/R391 Family That Encode Novel Diguanylate Cyclases Participate to C-Di-GMP Signalling in Vibrio Cholerae
    RÉGULATION DU C-DI-GMP ET RÔLE DE CE MESSAGER SECONDAIRE DANS LA FORMATION DE PILI DE TYPE IV CHEZ CLOSTRIDIUM DIFFICILE par Eric Bordeleau thèse présentée au Département de biologie en vue de l’obtention du grade de docteur ès science (Ph.D.) FACULTÉ DES SCIENCES UNIVERSITÉ DE SHERBROOKE Sherbrooke, Québec, Canada, juillet 2014 Le 15 juillet 2014 le jury a accepté la thèse de Monsieur Eric Bordeleau dans sa version finale. Membres du jury Professeur Vincent Burrus Directeur de recherche Département de biologie, Université de Sherbrooke Professeur Josée Harel Évaluatrice externe Département de biomédecine vétérinaire, Université de Montréal Professeur Daniel Lafontaine Évaluateur interne Département de biologie, Université de Sherbrooke Professeur Louis-Charles Fortier Évaluateur interne Département de microbiologie, Université de Sherbrooke Professeur François Malouin Président-rapporteur Département de biologie, Université de Sherbrooke SOMMAIRE Malgré la découverte du c-di-GMP en 1987, ce n’est que durant la dernière décennie que l’importance de ce messager secondaire dans la régulation des phénotypes bactériens a été exposée. Synthétisé par des diguanylate cyclases (DGC) et dégradé par des phosphodiestérases spécifiques (PDE), le c-di-GMP est prédit pour être un messager secondaire très répandu chez les bactéries et pratiquement exclusif à celles-ci. Le c-di-GMP est particulièrement reconnu pour son rôle dans la transition des bactéries motiles et planctoniques vers la formation de biofilm chez les bactéries à Gram négatif telles qu’Escherichia coli, Pseudomonas aeruginosa et Vibrio cholerae. De plus, le c-di-GMP est impliqué dans la régulation de l’expression de certains facteurs de virulence chez certaines bactéries.
    [Show full text]
  • Theologians As Persons in Dante's Commedia Abigail Rowson
    Theologians as Persons in Dante’s Commedia Abigail Rowson Submitted in accordance with the requirements for the degree of Doctor of Philosophy University of Leeds School of Languages, Cultures and Societies January 2018 The candidate confirms that the work submitted is her own and that appropriate credit has been given where reference has been made to the work of others. This copy has been supplied on the understanding that it is copyright material and that no quotation from the thesis may be published without proper acknowledgement. The right of Abigail Rowson to be identified as Author of this work has been asserted by Abigail Rowson in accordance with the Copyright, Designs and Patents Act 1988. 2 Acknowledgements Firstly, I am indebted to my supervisors, Claire Honess and Matthew Treherne, whose encouragement and support at the outset of this project gave me the confidence to even attempt it. I hope this work repays at least some of the significant amount of trust they placed in me; they should know that I shall be forever grateful to them. Secondly, this thesis would not have taken the shape it has without some wonderful intellectual interlocutors, including the other members of the Leeds/Warwick AHRC project. I feel fortunate to have been part of this wider intellectual community and have benefited enormously by being one of a team. I began to develop the structure and argument of the thesis at the University of Notre Dame’s Summer Seminar on Dante’s Theology, held at Tantur Ecumenical Institute, Jerusalem, 2013. The serendipitous timing of this event brought me into contact with an inspirational group of Dante scholars and theologians, whose generosity and intellectual humility was the hallmark of the fortnight.
    [Show full text]
  • DOTTORATO DI RICERCA Fonti Scritte Della Civiltà Mediterranea I
    Università degli Studi di Cagliari DOTTORATO DI RICERCA Fonti Scritte della Civiltà Mediterranea Ciclo XXVII I CANONICI OSPEDALIERI DI S. ANTONIO DI VIENNE E LA SARDEGNA Settore scientifico disciplinare di afferenza M‐STO/09 – Paleografia Presentata da: Mariangela Rapetti Coordinatore Dottorato Prof.ssa Giovanna Granata Tutor Prof.ssa Bianca Fadda Esame finale anno accademico 2013 – 2014 INDICE GENERALE Introduzione p. 3 Cap. I L’ordine di S. Antonio di Vienne tra fonti scritte e leggenda 1. Il problema delle fonti p. 8 2. Le origini: da fraternità laica a ordine canonicale p. 13 3. Un ordine fortemente gerarchizzato p. 19 4. Il culto del santo p. 30 Cap. II L’ordine di S. Antonio di Vienne in Sardegna: Status quaestionis 1. Storici antoniani p. 36 2. Gli studi in Sardegna p. 41 Cap. III Fonti per la storia antoniana di Sardegna 1. Gli antichi archivi antoniani p. 58 2. Archivi di Stato italiani p. 61 3. L’Archivio Segreto Vaticano e gli archivi ecclesiastici p. 63 4. Archivo de la Corona de Aragón p. 64 5. Tavola dei documenti consultati nel corso della ricerca p. 65 Cap. IV Gli insediamenti antoniani in Sardegna 1. La domus sive preceptoria Sardiniae p. 80 2. Oristano p. 87 3. Sassari p. 93 4. Cagliari p. 102 5. Altre località p. 108 Cap. V Le attività dei canonici antoniani: l’economia 1. La raccolta delle questue e l’amministrazione delle proprietà p. 112 2. L’allevamento dei maiali p. 117 1 Cap. VI Le attività dei canonici antoniani: l’assistenza 1. L’assistenza nelle strutture antoniane dalle origini alla riforma del 1478 p.
    [Show full text]
  • Macrinus and Diadumenianus
    Macrinus and Diadumenianus BY HENRY JEWELL BASSETT A DISSERTATION SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY IN THE UNIVERSITY OF MICHIGAN Macrinus and Diadumenianus BY HENRY JEWELL BASSETT A DISSERTATION SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY IN THE UNIVERSITY OF MICHIGAN QU|« OloIUgtst* ^rcM Geokge Banta Publishing Company Menasha, Wisconsin 1920 Digitized by the Internet Archive in 2014 https://archive.org/details/macrinusdiadumenOObass CHAPTER I The Inscriptions and Coins The sources of our information for the lives of Macrinus and Diadumenianus may be divided into three classes, viz.: literary, inscriptional and numismatic. The literary sources are Cassius Dio, Herodian, the Scriptores Historiae Augustae, and brief references in Aurelius Victor, Eutropius and Eusebius.^ These authorities will be treated in detail in a later chapter. The inscriptional evidence is quite extensive considering the shortness of Macrinus' reign, the fact that it was spent almost entirely in the Eastern provinces and that after his downfall his inscriptions were systematically erased. In Rome itself has been found the partially erased record in the Fasti sodalium Augustalium Claudialium recording the admission of Macrinus to that body (C.I.L. VI, 1984 (2), lines 13-17). The Fasti sacerdotum in aede Jovis Propugnatoris consistentium, while the stone has been broken and the letters erased, show by the context that they also contained the record of the election of Macrinus (C.I.L. VI, 2009). Lead pipes inscribed when Macrinus was pre- torian prefect, and others after his accession that bear his own titles with those of Diadumenianus (C.I.L.
    [Show full text]
  • Trio to Present Concert Mr
    Volume 11, Number 5 Published by Students of New College, Sarasota, Florida October 22, l96S College To Host U N Debate Trio To Present Concert Mr. Jacques Abram, Miss Patricia Stenberg, and Mr. Paul C. Wolfe will present a free concert as part of the Humanities program in the Music The Manatee County Chapter of Room at 8:00pm on October 25. the Unitec.l Nations Association is They will play piano, oboe and violin, respectively. sponsoring a debate to be held in On the program for the concen- are: Mozart's Sonata 4 in E Minor, the Music Room at 3:00pm tomor­ "'OW, October 23. "Brahms' Sonata in G Major, Opus 78, Quantz's Trio Sonata in G Ma­ The topic for the debate is RE­ Language Workshop JOr, and Mozart's Sonata 15 in B SOLVED: The United Nations Major. should be maintained. Here Jacques Abram, well-known con­ Speaker for the affirmative is Mr. Will Convene cert piani t and visiting professor Thomas P. Hardeman of Bradenton, ew College will host a workshop of music at New College, is a na­ former Vice President of the United for the Modern Language Section tive of Texas. At the age of ten Nations Association for Florid<l. of the Florida Educational Associa­ he held a scholarship to the Curtis tion, October 28, 29, and 30. Institute and at fourteen he rec­ eived a fellowship at the Julliard. Dr. George Mayer, visiting pro­ Teachers and graduate students fessor of history at New College, is attend the workshop to learn more School of Music in New York.
    [Show full text]
  • Resurveying the Religious Topography of Tiber Island
    RESURVEYING THE RELIGIOUS TOPOGRAPHY OF THE TIBER ISLAND By WILLIAM NOLAN BRUCE A THESIS PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF ARTS UNIVERSITY OF FLORIDA 2004 This thesis is dedicated to Mom, Dad, Clay, William G. & Anna Bevis, and ID & Annie- Mell Bruce. TABLE OF CONTENTS page LIST OF FIGURES .............................................................................................................v ABSTRACT....................................................................................................................... vi INTRODUCTION ...............................................................................................................1 The Island and Its Importance ......................................................................................1 Previous Work ..............................................................................................................2 CHAPTER 1 GENERAL BACKGROUND......................................................................................6 Legend of the Island's Origin........................................................................................6 Legend of Aesculapius' Arrival from Epidauros ..........................................................9 General information on Aesculapius and his Cult......................................................12 Epidauros as a Model for the Tiber Island and Other Asclepiea................................13 Temple.................................................................................................................13
    [Show full text]
  • Il Caso Di Sant'antonio Abate
    IMMAGINI E TESTI DA ALCUNI CICLI AD AFFRESCO DI PRIMO QUATTROCENTO NELL’ALTO CASERTANO: IL CASO DI SANT’ANTONIO ABATE ITAL I A CARADONNA * La presenza sul territorio dell’Alto Casertano di alcuni cicli ad affresco riferibili alla prima metà del Quattrocento raffiguranti le Storie di sant’Antonio abate offre lo spunto per una riflessione sulle fonti agiografiche da cui sono tratte le occorrenze iconografiche e per un’analisi dell’evoluzione dell’ico- nografia del santo. L’apporto degli antoniani è stato di fondamentale importanza per la costruzione dell’immagine di Antonio abate, e non è un caso se la loro ascesa procede di pari passo con la diffusione del culto del patrono. Un culto che vanta estensione europea e mediterranea e che ha nella repentina diffusione della devozione verso Antonio da Padova il suo momento di maggior declino. In the Alto Casertano area, we can find some fifteenth-century fresco cycles depicting Le Storie di Sant'Antonio Abate. These frescoes give us the opportunity to reflect on the iconographic occurrences of Sant’Antonio and their hagiographic sources. Moreover, the fresco cycles permit to analyse the iconographic evolution of the Saint. The contribution of Antonines had been important in the creation of Antonio Abate’s image and it is not a coincidence if their rise progressed at the same pace as the spread of its cult. This cult expanded across Europe and the Mediterranean area, however, the increas- ing devotion to Sant’Antonio da Padova caused its decline. In Campania, nella provincia di Caserta, tre cicli ad affresco narrano le vicende di un santo il cui culto è ampiamente diffuso sul territorio in esame: sant’Antonio abate1.
    [Show full text]
  • Anllech an Aolt an Boer an C an Cash an Caskey An
    BUSCAPRONTA www.buscapronta.com ARQUIVO 08 DE PESQUISAS GENEALÓGICAS 61 PÁGINAS – MÉDIA DE 19.600 SOBRENOMES/OCORRÊNCIA Para pesquisar, utilize a ferramenta EDITAR/LOCALIZAR do WORD. A cada vez que você clicar ENTER e aparecer o sobrenome pesquisado GRIFADO (FUNDO PRETO) corresponderá um endereço Internet correspondente que foi pesquisado por nossa equipe. Ao solicitar seus endereços de acesso Internet, informe o SOBRENOME PESQUISADO, o número do ARQUIVO BUSCAPRONTA DIV ou BUSCAPRONTA GEN correspondente e o número de vezes em que encontrou o SOBRENOME PESQUISADO. Número eventualmente existente à direita do sobrenome (e na mesma linha) indica número de pessoas com aquele sobrenome cujas informações genealógicas são apresentadas. O valor de cada endereço Internet solicitado está em nosso site www.buscapronta.com . Para dados especificamente de registros gerais pesquise nos arquivos BUSCAPRONTA DIV. ATENÇÃO: Quando pesquisar em nossos arquivos, ao digitar o sobrenome procurado, faça- o, sempre que julgar necessário, COM E SEM os acentos agudo, grave, circunflexo, crase, til e trema. Sobrenomes com (ç) cedilha, digite também somente com (c) ou com dois esses (ss). Sobrenomes com dois esses (ss), digite com somente um esse (s) e com (ç). (ZZ) digite, também (Z) e vice-versa. (LL) digite, também (L) e vice-versa. Van Wolfgang – pesquise Wolfgang (faça o mesmo com outros complementos: Van der, De la etc) Sobrenomes compostos ( Mendes Caldeira) pesquise separadamente: MENDES e depois CALDEIRA. Tendo dificuldade com caracter Ø HAMMERSHØY – pesquise HAMMERSH HØJBJERG – pesquise JBJERG BUSCAPRONTA não reproduz dados genealógicos das pessoas, sendo necessário acessar os documentos Internet correspondentes para obter tais dados e informações. DESEJAMOS PLENO SUCESSO EM SUA PESQUISA.
    [Show full text]
  • Anna Esposito / Andreas Rehberg (Hrsg.): Gli Ordini Ospedalieri
    Anna Esposito / Andreas Rehberg (Hrsg.): Gli ordini ospedalieri tra centro e periferia Schriftenreihe Ricerche dell'Istituto Storico Germanico di Roma Band 3 (2007) Herausgegeben vom Deutschen Historischen Institut Rom Copyright Das Digitalisat wird Ihnen von perspectivia.net, der Online-Publikationsplattform der Max Weber Stiftung – Deutsche Geisteswissenschaftliche Institute im Ausland, zur Verfügung gestellt. Bitte beachten Sie, dass das Digitalisat der Creative- Commons-Lizenz Namensnennung-Keine kommerzielle Nutzung-Keine Bearbeitung (CC BY-NC-ND 4.0) unterliegt. Erlaubt ist aber das Lesen, das Ausdrucken des Textes, das Herunterladen, das Speichern der Daten auf einem eigenen Datenträger soweit die vorgenannten Handlungen ausschließlich zu privaten und nicht-kommerziellen Zwecken erfolgen. Den Text der Lizenz erreichen Sie hier: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode Ricerche , dell Istituto Storico Germanico di Roma , 1. Barbara Bombi Gli ordini ospedalieri tra centro e periferia Ricerche dell Istituto Storico Il registro di Andrea Sapiti, Gli ordini ospedalieri tra centro e periferia procuratore alla curia avignonese A cura di Anna Esposito e Andreas Rehberg Germanico di Roma 2007, 412 pp. Rispetto ad altre forme di vita religiosa (ordini monastici, ordini men- L’Istituto Storico Germanico di Roma, 2. Nicola D’Elia dicanti etc.) gli ordini ospedalieri – tra cui spiccano gli Anto-niani, le fondato nel 1888, ha finalità di ricerca sulla Delio Cantimori e la cultura comunità di S. Giacomo di Altopascio vicino Lucca, di S. Maria di storia italiana e tedesca dall’alto medioevo politica tedesca (1927-1940) Roncisvalle in Navarra e di S. Spirito in Sassia a Roma – non hanno fino al passato più recente.
    [Show full text]
  • Coinage in Late Hellenistic and Roman Syria: the Orontes Valley (1 St Century Bc - 3Rd Century Ad)
    University of Warwick institutional repository: http://go.warwick.ac.uk/wrap A Thesis Submitted for the Degree of PhD at the University of Warwick http://go.warwick.ac.uk/wrap/57324 This thesis is made available online and is protected by original copyright. Please scroll down to view the document itself. Please refer to the repository record for this item for information to help you to cite it. Our policy information is available from the repository home page. COINAGE IN LATE HELLENISTIC AND ROMAN SYRIA: THE ORONTES VALLEY (1 ST CENTURY BC - 3RD CENTURY AD) by Jack Antoine Nurpetlian A thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Classics and Ancient History University of Warwick, Department of Classics and Ancient History February 2013 CONTENTS CONTENTS ........................................................................................................................................II ABBREVIATIONS ........................................................................................................................... XIII CHAPTER I: INTRODUCTION ............................................................................................................. 1 A. NUMISMATIC BACKGROUND ................................................................................................................... 3 B. GEOGRAPHICAL BACKGROUND ................................................................................................................. 5 C. HISTORICAL BACKGROUND .....................................................................................................................
    [Show full text]
  • Raffaela Villamena: I Cerretani Come Intermediari Degli Antoniani (A
    Raffaela Villamena: I Cerretani come intermediari degli Antoniani (a proposito di due documenti del 1315 e del 1492) Schriftenreihe Ricerche dell'Istituto Storico Germanico di Roma Band 3 (2007) Herausgegeben vom Deutschen Historischen Institut Rom Copyright Das Digitalisat wird Ihnen von perspectivia.net, der Online-Publikationsplattform der Max Weber Stiftung – Deutsche Geisteswissenschaftliche Institute im Ausland, zur Verfügung gestellt. Bitte beachten Sie, dass das Digitalisat der Creative- Commons-Lizenz Namensnennung-Keine kommerzielle Nutzung-Keine Bearbeitung (CC BY-NC-ND 4.0) unterliegt. Erlaubt ist aber das Lesen, das Ausdrucken des Textes, das Herunterladen, das Speichern der Daten auf einem eigenen Datenträger soweit die vorgenannten Handlungen ausschließlich zu privaten und nicht-kommerziellen Zwecken erfolgen. Den Text der Lizenz erreichen Sie hier: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode Raffaela Villamena I Cerretani come intermediari degli Antoniani (a proposito di due documenti del 1315 e del 1492) Per l’ordine dei canonici regolari di Sant’Agostino di Sant’Antonio di Vienne l’analisi dei rapporti tra autorità centrale – l’abbaye di Sant’Antonio presso Vienne – e periferia – le precettorie sparse in tutta la Cristianità 1 –non può prescindere dall’esame del ruolo che i collettori di questue, per la quasi totalità provenienti da Cerreto di Spoleto, ricoprivano all’interno dell’econo- mia antoniana. Il mio intervento non si soffermerà tanto sulla natura ambigua di questi personaggi e non indugerà sul
    [Show full text]
  • Ordine Degli Avvocati Di Campobasso Eugp
    ORDINE DEGLI AVVOCATI DI CAMPOBASSO EUGP - Elenco Unico Gratuito Patrocinio (NP) Elenco degli Avvocati disponibili al Patrocinio a spese dello Stato Aggiornato al 28 giugno 2019 28/06/2019 2 / 60 ORDINE DEGLI AVVOCATI DI CAMPOBASSO ELENCO ISCRITTI Cognome e nome Arch./Pref. Titolare dello studio oppure Dominus per i praticanti Data adesione Cellulare Indirizzi studio / residenza / eventuale altro recapito / e-mail / PEC Telefoni Fax Avv. ABBAZIA Manuela 1 / 771 3393245432 EUGP - Elenco Unico del Gratuito Patrocinio - Ades.: 02/02/2011 Ind. 1: CAMPOBASSO - 86100 - Via Roma 48 087497413 087466825 [email protected] [email protected] Avv. ALBANESE Luciana 1 / 999 3492949357 EUGP - Elenco Unico del Gratuito Patrocinio - Ades.: 02/02/2015 Ind. 1: CAMPOBASSO - 86100 - Via Alfredo Trombetta 36 087463629 087463629 [email protected] [email protected] Avv. ALBINIANO Francesca 1 / 1117 3286721148 EUGP - Elenco Unico del Gratuito Patrocinio - Ades.: 05/02/2019 Ind. 1: CAMPOBASSO - 86100 - C.da San Giovanni In Golfo 278 08741896065 0874699205 [email protected] [email protected] Avv. ALBINO Eduardo 1 / 1 3332748352 EUGP - Elenco Unico del Gratuito Patrocinio - Ades.: 12/01/2007 Ind. 1: CAMPOBASSO - 86100 - Viale Principe di Piemonte, 22 087490185 [email protected] * Avv. ALBINO Fabio 1 / 2 3391885089 EUGP - Elenco Unico del Gratuito Patrocinio - Ades.: 12/01/2007 Ind. 1: CAMPOBASSO - 86100 - Viale P. di Piemonte 22 087490468 087490468 [email protected] [email protected] Avv. AMOROSO Angela 1 / 702 3284596719 EUGP - Elenco Unico del Gratuito Patrocinio - Ades.: 11/12/2008 Ind. 1: LIMOSANO - 86022 - Via Garibaldi 65 0874701470 0874701470 [email protected] [email protected] * Avv.
    [Show full text]