4. Ingredient Sources, Composition and Reported Usage
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29 4. Ingredient sources, composition and reported usage It must be stated at the outset that the current review of feed ingredient sources and reported usage within compound aquafeeds is based upon an analysis of published information and papers in the public domain. For the most part these are feeding studies conducted by university/government researchers usually under controlled laboratory conditions, typically with juvenile animals over a fixed 8- to 16-week time period. Apart from the difficulty of extrapolating the findings of these laboratory- based research studies to outdoor commercial farming conditions, the nutrient content and nutritional value of individual feed ingredient sources varies considerably between countries and ingredient processing facilities depending on local farming conditions and processing methods employed. Moreover, the ultimate performance of a feed ingredient within a formulated aquafeed will depend on the dietary formulation employed, including the nutrient profile of the diet fed and the level of the feed ingredient used and ambient rearing conditions, including natural food availability in the case of pond-reared animals (Tacon, 1995, 1996). Despite the above limitations, some generalizations can be made regarding the nutrient composition and reported usage of individual feedstuffs within compound aquafeeds. The current review covers information gained from feeding studies conducted after 1994; studies conducted prior to that date having been reviewed previously (see Tacon, 1993a, 1993b, 2004). 4.1 ANIMAL PROTEIN SOURCES 4.1.1 Fishery products Official definitions (AAFCO, 2008b) Condensed fish protein digest (IFN 5-17-779 Fish protein hydrolysed condensed) is the condensed enzymatic digest of clean undecomposed whole fish or fish cuttings using the enzyme hydrolysis process. The product must be free of bones, scales and undigested solids with or without the extraction of part of the oil. It must contain not less than 30 percent protein. Condensed fish solubles is obtained by evaporating excess moisture from the stickwater, aqueous liquids, resulting from the wet rendering of fish into fishmeal, with or without removal of part of the oil. Minimum percent of solids, minimum percent of crude protein and minimum percent of crude fat must be guaranteed. Crab meal (IFN 5-01-663 Crab process residue meal) is the undecomposed ground dried waste of the crab and contains the shell, viscera and part or all of the flesh. It must contain not less than 25 percent crude protein. If it contains more than 3 percent salt (NaCl), the amount of salt must constitute a part of the product name, although in no case must the salt content of this product exceed 7 percent. Dried fish protein digest (IFN 5-18-778 Fish protein hydrolysed dehydrated) is the dried enzymatic digest of clean undecomposed whole fish or fish cuttings using the enzyme hydrolysis process. The product must be free of bones, scales and undigested solids with or without the extraction of part of the oil. It must contain not less than 80 percent protein and not more than 10 percent moisture. If the degree of fineness is stated, it must conform thereto. 30 Feed ingredients and fertilizers for farmed aquatic animals – Sources and composition Dried fish solubles (IFN 5-01-971 Fish solubles dehydrated) is obtained by dehydrating the stickwater. It must contain not less than 60 percent crude protein. Dried shellfish digest is the dried enzymatic digest of clean, undecomposed shellfish (crustaceans and/or molluscs), using the enzyme hydrolysis process. The product may contain shells, viscera and part or all of the flesh, and must be free of undigested solids with or without the extraction of part of the oil. It must contain not less than 50 percent crude protein with not more than 10 percent moisture. If the degree of fineness is stated, it must conform thereto. If the product bears a name descriptive of its kind, composition or origin, it must correspond thereto. Fish by-products (IFN 5-14-509 Fish process residue fresh) must consist of non- rendered, clean undecomposed portions of fish (such as, but not limited to, heads, fins, tails, ends, skin, bone and viscera) which result from the fish processing industry. If it bears a name descriptive of its kind, it must correspond thereto. Any single constituent used as such may be labeled according to the common or usual name of the particular portion used (such as fish heads, fish tails, etc.). Fish digest residue (IFN 5-27-467 Fish protein residue hydrolyzed dehydrated) is the clean, dried, undecomposed residue (bones-scales-undigested solids) of the enzymatic digest resulting from the enzyme hydrolysis process of producing fish protein digest. It must be designated according to its protein, calcium and phosphorus content. Fish liver and glandular meal (IFN 5-01-973 Fish viscera meal) is obtained by drying the complete viscera of the fish. At least 50 percent of the dry weight of the product must be derived from fish liver and must contain at least 18 milligrams of riboflavin per pound (Adopted 1944, Amended 1945; AAFCO, 2008b). Fishmeal (IFN 5-01-977 Fishmeal mechanical extracted) is the clean, dried, ground tissue of undecomposed whole fish or fish cuttings, either or both, with or without the extraction of part of the oil. If it contains more than 3 percent salt (NaCl), the amount of salt must constitute a part of the product name, although in no case must the salt content of this product exceed 7 percent. The label shall include guarantees for minimum crude protein, minimum crude fat, maximum crude fibre, minimum phosphorus (P) and minimum and maximum calcium (Ca). If it bears a name descriptive of its kind, it must correspond thereto. Fish protein concentrate – feed grade (IFN 5-09-334 Fish protein concentrate solvent extracted) is prepared from clean, undecomposed whole fish or fish cuttings using the solvent extraction process developed for the production of edible whole fish protein concentrate. It must contain not Iess than 70 percent protein and not more than 10 percent moisture. If the degree of fineness is stated, it must conform thereto. Solvent residues are not to exceed those established in Food Additive Regulations. Fish residue meal (IFN 5-01-966 Fish glue residue meal) is the clean, dried, undecomposed residue from the manufacture of glue from non-oily fish. If it contains more than 3 percent salt (NaCl), the amount of salt must constitute a part of the product name, although in no case must the salt content of this product exceed 7 percent. Fish stock/broth is obtained by cooking fish and/or other marine animal products, including bones, shells, parts and/or muscle, but not including fish solubles. The crude protein content of the stock/broth base material must be no less than 90 percent on a dry matter basis. In order for the stock/broth to be labeled as such, the moisture-to- Ingredient sources, composition and reported usage 31 crude protein ration must not exceed 135:1 (135 parts water to 1 part crude protein). If the product bears a name descriptive of its kind, composition or origin, it must correspond thereto; and may be called either stock or broth. Shrimp meal (IFN 5-04-226 Shrimp process residue meal) is the undecomposed, ground dried waste of shrimp and contains parts and/or whole shrimp. If it contains more than 3 percent salt (NaCl), the amount of salt must constitute a part of the product name, although in no case must the salt content of this product exceed 7 percent. Reported proximate and essential amino acid composition The average reported proximate and essential amino acid composition of the major fishery products most commonly used in compound aquafeeds is shown in Table 15 and 16, respectively. In general, fishery products are good sources of essential dietary nutrients for most farmed finfish and crustaceans, with the nutrient profile of whole processed meals approximating very closely to the known dietary nutrient requirements, in particular for carnivorous finfish and crustacean species. This is particularly true for the essential amino acids (Table 16) and other essential nutrients, TABLE 15 Reported proximate composition of selected fish products – values expressed as % as-fed basis; Water-H2O; Crude Protein-CP; Lipid or Ether Extract-EE; Crude Fibre-CF; Ash; Calcium-Ca; Phosphorus-P 1 Fish product H2O CP EE CF Ash Ca P Ref. Fishmeals – mechanically extracted, fish silages and hydrolysates Anchovy (5-01-985) min 7.1 65.3 4.1 0.8 14.8 3.75 1.42 1,2,3, max 8.3 68.3 10.0 1.3 17.3 4.03 2.49 11,22 mean 7.9 66.7 6.8 1.0 15.9 3.89 1.95 Menhaden (5-02-009) min 3.8 61.1 9.3 0.9 19.0 5.11 2.89 1,3,4, max 8.0 67.7 10.7 1.0 21.5 6.89 3.65 22 mean 6.5 63.4 9.9 0.9 19.9 5.73 3.15 Herring (5-02-009) min 7.9 72.0 8.4 0.7 10.1 2.04 1.42 1,3, max 8.0 72.7 8.5 0.8 10.5 2.20 1.68 22 mean 7.9 72.3 8.4 0.7 101.3 2.12 1.55 Tuna (5-02-023) min 7.0 59.0 6.9 0.8 17.0 7.86 4.21 1,2, max 9.4 65.4 8.0 0.8 21.9 7.86 4.21 15,22 mean 8.2 62.2 7.4 0.8 19.4 7.86 4.21 Sardine min 7.0 59.0 6.7 0.3 14.2 4.44 2.72 3,11 max 8.5 65.0 9.1 1.0 15.3 - - mean 7.7 62.0 7.9 0.6 14.7 4.44 2.72 Horse/Jack mackerel min 4.6 66.6 9.0 - 13.7 - - 5,9 max 7.7 70.0 13.1 - 13.9 - - 20 mean 6.1 68.1 11.0 - 13.8 - - White (5-02-025) min 6.5 62.2 4.2 0.2 18.0 6.84 3.80 1,2,3 max 10.0 69.0 7.6 0.9 23.7 8.0 4.80 5,6, mean 8.3 64.5 5.1 0.6 21.3 7.4 4.12 15 Alaskan pollock (from processing waste) min 3.4 65.2 5.0 - 10.1 2.67 1.70 7,8 max 8.0 74.3 11.3 - 23.5 8.51 4.39 mean 5.9 69.0 7.6 - 17.3 5.87 3.23 Cod (from