Supplementary Material Isolation, Identification, Optimization and metabolite profiling of Streptomyces sparsus VSM-30 Ushakiranmayi Managamuri1, Muvva Vijayalakshmi1*, V.S Rama Krishna Ganduri 2, 4, Satish Babu R3, Bodaiah B4, B.S.Kalyani5, Sudhakar Poda4

1Department of Botany and Microbiology, Acharya Nagarjuna University, Nagarjuna nagar, Guntur-52510, Andhra Pradesh, India. 2Department of Biotechnology, K L University, Vaddeswaram, Guntur, Andhra Pradesh, India. 3Dept of Biotechnology, National Institute of Technology, Warangal, Telangana, India. 4Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna nagar, Guntur-52510, Andhra Pradesh, India. 5Department of Microbiology, D.N.R.College, Bhimavaram, A.P., India

Supplementary Table 1 Central composite factor experimental design along with experimental and predicted values

Run A- B-pH C- D- E-Soya A.niger F.oxysporum F.solani P.citrinum Time Tem Sucrose Peptone Actual RS Actual RSM Actual RS Actual RSM Actual RSM p M M erat ure 1 11.00 9.00 25.0 1.50 0.50 18.00 18.2 18.00 17.92 16.00 15.92 16.00 16.01 18.00 18.40 2 13.00 7.00 035.0 1.50 1.50 19.00 618.7 18.00 17.93 16.00 15.92 16.00 16.01 18.00 17.87 3 11.00 7.00 035.0 1.50 1.50 17.00 417.2 17.00 16.92 15.00 15.16 16.00 15.98 18.00 17.63 4 12.00 7.00 030.0 1.00 1.00 18.00 617.7 17.00 16.93 15.00 15.16 16.00 15.98 17.00 17.10 5 12.00 8.00 030.0 1.00 1.00 19.00 418.7 18.00 18.16 16.00 15.92 16.00 16.01 19.00 18.63 6 11.00 9.00 025.0 1.50 1.50 19.00 419.4 18.00 17.92 16.00 15.92 16.00 16.01 18.00 18.10 7 13.00 8.00 030.0 1.00 1.00 18.00 617.7 17.00 17.16 15.00 15.16 16.00 15.98 18.00 17.87 8 11.00 9.00 035.0 1.50 0.50 18.00 418.4 17.00 16.92 15.00 15.16 16.00 15.98 17.00 17.34 9 11.00 7.00 035.0 1.50 0.50 18.00 617.9 18.00 17.93 16.00 16.13 16.00 16.03 18.00 18.16 10 13.00 7.00 035.0 1.50 0.50 18.00 718.2 18.00 18.18 16.00 16.13 16.00 16.03 18.00 17.63 11 12.00 9.00 030.0 1.00 1.00 17.00 016.9 17.00 16.93 15.00 14.86 15.00 15.01 17.00 17.40 12 12.00 8.00 030.0 1.00 1.50 17.00 717.2 17.00 17.18 15.00 14.86 15.00 15.01 17.00 16.87 13 12.00 8.00 025.0 1.00 1.00 18.00 018.2 18.00 17.92 16.00 16.13 16.00 16.03 18.00 18.40 0 0 14 12.00 8.00 30.0 1.00 1.00 19.00 18.6 18.00 17.93 16.00 16.13 16.00 16.03 18.00 17.87 15 11.00 7.00 025.0 1.50 1.50 17.00 817.2 17.00 16.92 15.00 14.86 15.00 15.01 18.00 17.63 16 11.00 8.00 030.0 1.00 1.00 18.00 017.6 17.00 16.93 15.00 14.86 15.00 15.01 17.00 17.10 17 12.00 8.00 030.0 1.50 1.00 18.00 817.9 17.00 17.22 15.00 15.16 15.00 15.01 18.00 17.87 18 12.00 8.00 030.0 1.00 1.00 18.00 718.2 17.00 16.98 15.00 15.16 15.00 15.01 17.00 17.34 19 13.00 9.00 035.0 0.50 0.50 17.00 016.9 16.00 16.22 15.00 14.89 15.00 14.98 17.00 17.10 20 11.00 7.00 025.0 0.50 0.50 17.00 717.2 16.00 15.98 15.00 14.89 15.00 14.98 17.00 16.57 21 13.00 9.00 035.0 1.50 1.50 18.00 018.2 18.00 17.71 15.00 15.16 15.00 15.01 18.00 18.10 22 13.00 9.00 035.0 1.50 0.50 19.00 018.6 17.00 17.22 15.00 15.16 15.00 15.01 18.00 17.57 23 11.00 7.00 035.0 0.50 0.50 17.00 817.2 17.00 16.71 15.00 14.89 15.00 14.98 17.00 17.34 24 12.00 8.00 030.0 1.00 1.00 18.00 017.6 16.00 16.22 15.00 14.89 15.00 14.98 17.00 16.81 25 13.00 9.00 025.0 1.50 0.50 18.00 817.9 17.00 16.98 15.00 14.86 15.00 15.03 18.00 17.63 26 13.00 7.00 025.0 0.50 1.50 18.00 317.9 17.00 16.98 15.00 14.86 15.00 15.03 17.00 17.10 27 11.00 9.00 025.0 0.50 0.50 17.00 116.9 16.00 15.98 14.00 14.10 14.00 14.01 17.00 16.87 28 13.00 9.00 025.0 1.50 1.50 17.00 316.9 16.00 15.98 14.00 14.10 14.00 14.01 16.00 16.34 29 13.00 7.00 035.0 0.50 1.50 18.00 117.9 17.00 17.22 15.00 14.86 15.00 15.03 18.00 17.87 30 11.00 9.00 035.0 1.50 1.50 18.00 118.1 17.00 16.98 15.00 14.86 15.00 15.03 17.00 17.34 31 13.00 7.00 025.0 0.50 0.50 17.00 416.9 16.00 16.22 14.00 14.10 14.00 14.01 17.00 17.10 32 12.00 8.00 030.0 1.00 1.00 17.00 117.1 16.00 15.98 14.00 14.10 14.00 14.01 17.00 16.57 33 12.00 8.00 030.0 1.00 0.50 21.00 420.6 19.00 18.90 18.00 17.85 19.00 18.92 21.00 20.99 34 13.00 9.00 035.0 0.50 1.50 21.00 420.9 19.00 18.78 18.00 17.85 19.00 18.92 20.00 20.46 35 11.00 7.00 025.0 0.50 1.50 21.00 920.8 19.00 18.84 18.00 17.73 19.00 18.68 20.00 20.10 36 11.00 9.00 035.0 0.50 0.50 20.00 119.8 18.00 17.84 17.00 16.97 18.00 18.15 19.00 19.34 37 12.00 8.00 030.0 1.00 1.00 21.00 120.6 19.00 18.78 18.00 17.85 19.00 18.92 20.00 20.10 38 12.00 8.00 030.0 1.00 1.00 21.00 420.9 19.00 18.90 18.00 17.85 19.00 18.92 20.00 20.34 39 11.00 7.00 035.0 0.50 1.50 21.00 920.5 19.00 18.90 18.00 17.50 18.00 18.15 20.00 20.34 40 11.00 9.00 035.0 0.50 1.50 20.00 220.1 19.00 18.78 17.00 17.20 18.00 17.68 20.00 20.10 41 11.00 9.00 025.0 0.50 1.50 21.00 120.5 19.00 19.25 18.00 17.73 18.00 17.92 20.00 19.99 42 12.00 8.00 035.0 1.00 1.00 20.00 220.1 19.00 18.43 17.00 16.97 17.00 16.92 19.00 19.46 43 13.00 7.00 035.0 0.50 0.50 21.00 121.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 44 13.00 9.00 025.0 0.50 0.50 21.00 921.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 45 13.00 9.00 025.0 0.50 1.50 21.00 921.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 0 9 46 12.00 8.00 30.0 0.50 1.00 21.00 21.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 47 13.00 7.00 025.0 1.50 1.50 21.00 921.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 48 11.00 7.00 025.0 1.50 0.50 21.00 921.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 49 12.00 8.00 030.0 1.00 1.00 21.00 921.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 50 13.00 7.00 025.0 1.50 0.50 21.00 921.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 0 9 Supplementary Table 2

S.N Molecular Molecula R.T Area SI Biological activity O Name of the compound formula r weight

C5H10N2 98 Antibacterial, Anti- 1H-Imidazole, 4,5-dihydro- inflammatory and 1 2,4-dimethyl- 3.25 3003683 97 antifungal

2 Neoisolongifolene, 8,9-epoxy- C5H22O 218 10.634 1151023 99 Unknown

3 1-Undecanol C11H24O 172 12.138 242041 99 Flavor, Perfumery

2- C17H27F7O2 396 Unknown 4 Heptafluorobutyroxytridecane 12.666 164494 99

Phenol, 2,4-bis(1,1- C17H30OSi 278 Antioxidant 5 dimethylethyl)- 18.704 1012005 99

1-Dodecanol, 3,7,11- C15H32O 228 Unknown 6 trimethyl- 18.819 133099 98

7 2-Hexyl-1-octanol C14H30O 214 19.634 105314 99 No activity reported

C10H22O3S 222 1198500 Unknown 8 Sulfurous acid, dipentyl ester 21.051 7 99

9 4-Trifluoroacetoxyhexadecane C18H33F3O2 338 23.685 4807929 99 Antimicrobial

10 1-Decanol, 2-hexyl- C16H34O 242 24.924 169440 99 Unknown

Butyl-4-amino-1-benzyl C19H28N2O6 380 Unknown piperidine -4-carboxylate, 11 oxalic acid 25.515 3307728 91

12 3-Octadecene, (E)- C18H36 252 25.687 444075 99 No activity reported

N-(4-Ethoxy-2-nitrophenyl) C10H12N2O4 174 Unknown 13 acetamide 25.912 4710746 97

Heptafluorobutanoic acid, C22H37F7O2 466 Unknown 14 octadecyl ester 26.271 93368 98

N-[4- Cyclooctylaminobutyl] C14H28N2 224 1652309 Unknown 15 aziridine 27.386 4 99

16 Benzo[c] Phenanthen-5-amine C18H13N 243 27.913 1797968 99 Unknown

Phthalic acid, ethyl pentyl C15H20 264 Antibacterial 17 ester 28.388 669591 98

18 L-Alanyl-L-Tryptophan C14H17N3O3 275 29.691 7728888 99 Antimicrobial

L-Proline, N- C15H25NO4 283 Unknown 19 allyloxycarbonyl-, hexyl ester 30.076 1715933 99

C13H25NSi 223 1777396 Unknown 20 1-(Triisopropylsilyl)pyrrole 30.555 1 99

21 Butyric acid, pentadecyl ester C19H38O2 298 31.883 567648 99 Analgesics 22 Isopropyl stearate C21H42O2 326 35.352 617715 98 Unknown  1- CH3(CH2)15S  Antifungal and Hexadecanesulfonyl O2Cl 324 Antioxidant chloride

23 37.018 148213 99

24 Diheptyl phthalate C22H34O4 362 43.282 600003 99 Antimicrobial

25 Dihydrovallesiachotamine C21H24N2O3 352 47.59 113599 89 Unknown

C32H66 450 Antitubercular, Antioxidant and 26 Dotriacontane 54.472 521116 98 Antispasmodic

C34H70 478 Antimicrobial and 27 Tetracosane, 11-decyl- 57.38 5285833 99 Antioxidant