<p> Supplementary Material Isolation, Identification, Optimization and metabolite profiling of Streptomyces sparsus VSM-30 Ushakiranmayi Managamuri1, Muvva Vijayalakshmi1*, V.S Rama Krishna Ganduri 2, 4, Satish Babu R3, Bodaiah B4, B.S.Kalyani5, Sudhakar Poda4</p><p>1Department of Botany and Microbiology, Acharya Nagarjuna University, Nagarjuna nagar, Guntur-52510, Andhra Pradesh, India. 2Department of Biotechnology, K L University, Vaddeswaram, Guntur, Andhra Pradesh, India. 3Dept of Biotechnology, National Institute of Technology, Warangal, Telangana, India. 4Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna nagar, Guntur-52510, Andhra Pradesh, India. 5Department of Microbiology, D.N.R.College, Bhimavaram, A.P., India</p><p>Supplementary Table 1 Central composite factor experimental design along with experimental and predicted values</p><p>Run A- B-pH C- D- E-Soya A.niger F.oxysporum F.solani P.citrinum Time Tem Sucrose Peptone Actual RS Actual RSM Actual RS Actual RSM Actual RSM p M M erat ure 1 11.00 9.00 25.0 1.50 0.50 18.00 18.2 18.00 17.92 16.00 15.92 16.00 16.01 18.00 18.40 2 13.00 7.00 035.0 1.50 1.50 19.00 618.7 18.00 17.93 16.00 15.92 16.00 16.01 18.00 17.87 3 11.00 7.00 035.0 1.50 1.50 17.00 417.2 17.00 16.92 15.00 15.16 16.00 15.98 18.00 17.63 4 12.00 7.00 030.0 1.00 1.00 18.00 617.7 17.00 16.93 15.00 15.16 16.00 15.98 17.00 17.10 5 12.00 8.00 030.0 1.00 1.00 19.00 418.7 18.00 18.16 16.00 15.92 16.00 16.01 19.00 18.63 6 11.00 9.00 025.0 1.50 1.50 19.00 419.4 18.00 17.92 16.00 15.92 16.00 16.01 18.00 18.10 7 13.00 8.00 030.0 1.00 1.00 18.00 617.7 17.00 17.16 15.00 15.16 16.00 15.98 18.00 17.87 8 11.00 9.00 035.0 1.50 0.50 18.00 418.4 17.00 16.92 15.00 15.16 16.00 15.98 17.00 17.34 9 11.00 7.00 035.0 1.50 0.50 18.00 617.9 18.00 17.93 16.00 16.13 16.00 16.03 18.00 18.16 10 13.00 7.00 035.0 1.50 0.50 18.00 718.2 18.00 18.18 16.00 16.13 16.00 16.03 18.00 17.63 11 12.00 9.00 030.0 1.00 1.00 17.00 016.9 17.00 16.93 15.00 14.86 15.00 15.01 17.00 17.40 12 12.00 8.00 030.0 1.00 1.50 17.00 717.2 17.00 17.18 15.00 14.86 15.00 15.01 17.00 16.87 13 12.00 8.00 025.0 1.00 1.00 18.00 018.2 18.00 17.92 16.00 16.13 16.00 16.03 18.00 18.40 0 0 14 12.00 8.00 30.0 1.00 1.00 19.00 18.6 18.00 17.93 16.00 16.13 16.00 16.03 18.00 17.87 15 11.00 7.00 025.0 1.50 1.50 17.00 817.2 17.00 16.92 15.00 14.86 15.00 15.01 18.00 17.63 16 11.00 8.00 030.0 1.00 1.00 18.00 017.6 17.00 16.93 15.00 14.86 15.00 15.01 17.00 17.10 17 12.00 8.00 030.0 1.50 1.00 18.00 817.9 17.00 17.22 15.00 15.16 15.00 15.01 18.00 17.87 18 12.00 8.00 030.0 1.00 1.00 18.00 718.2 17.00 16.98 15.00 15.16 15.00 15.01 17.00 17.34 19 13.00 9.00 035.0 0.50 0.50 17.00 016.9 16.00 16.22 15.00 14.89 15.00 14.98 17.00 17.10 20 11.00 7.00 025.0 0.50 0.50 17.00 717.2 16.00 15.98 15.00 14.89 15.00 14.98 17.00 16.57 21 13.00 9.00 035.0 1.50 1.50 18.00 018.2 18.00 17.71 15.00 15.16 15.00 15.01 18.00 18.10 22 13.00 9.00 035.0 1.50 0.50 19.00 018.6 17.00 17.22 15.00 15.16 15.00 15.01 18.00 17.57 23 11.00 7.00 035.0 0.50 0.50 17.00 817.2 17.00 16.71 15.00 14.89 15.00 14.98 17.00 17.34 24 12.00 8.00 030.0 1.00 1.00 18.00 017.6 16.00 16.22 15.00 14.89 15.00 14.98 17.00 16.81 25 13.00 9.00 025.0 1.50 0.50 18.00 817.9 17.00 16.98 15.00 14.86 15.00 15.03 18.00 17.63 26 13.00 7.00 025.0 0.50 1.50 18.00 317.9 17.00 16.98 15.00 14.86 15.00 15.03 17.00 17.10 27 11.00 9.00 025.0 0.50 0.50 17.00 116.9 16.00 15.98 14.00 14.10 14.00 14.01 17.00 16.87 28 13.00 9.00 025.0 1.50 1.50 17.00 316.9 16.00 15.98 14.00 14.10 14.00 14.01 16.00 16.34 29 13.00 7.00 035.0 0.50 1.50 18.00 117.9 17.00 17.22 15.00 14.86 15.00 15.03 18.00 17.87 30 11.00 9.00 035.0 1.50 1.50 18.00 118.1 17.00 16.98 15.00 14.86 15.00 15.03 17.00 17.34 31 13.00 7.00 025.0 0.50 0.50 17.00 416.9 16.00 16.22 14.00 14.10 14.00 14.01 17.00 17.10 32 12.00 8.00 030.0 1.00 1.00 17.00 117.1 16.00 15.98 14.00 14.10 14.00 14.01 17.00 16.57 33 12.00 8.00 030.0 1.00 0.50 21.00 420.6 19.00 18.90 18.00 17.85 19.00 18.92 21.00 20.99 34 13.00 9.00 035.0 0.50 1.50 21.00 420.9 19.00 18.78 18.00 17.85 19.00 18.92 20.00 20.46 35 11.00 7.00 025.0 0.50 1.50 21.00 920.8 19.00 18.84 18.00 17.73 19.00 18.68 20.00 20.10 36 11.00 9.00 035.0 0.50 0.50 20.00 119.8 18.00 17.84 17.00 16.97 18.00 18.15 19.00 19.34 37 12.00 8.00 030.0 1.00 1.00 21.00 120.6 19.00 18.78 18.00 17.85 19.00 18.92 20.00 20.10 38 12.00 8.00 030.0 1.00 1.00 21.00 420.9 19.00 18.90 18.00 17.85 19.00 18.92 20.00 20.34 39 11.00 7.00 035.0 0.50 1.50 21.00 920.5 19.00 18.90 18.00 17.50 18.00 18.15 20.00 20.34 40 11.00 9.00 035.0 0.50 1.50 20.00 220.1 19.00 18.78 17.00 17.20 18.00 17.68 20.00 20.10 41 11.00 9.00 025.0 0.50 1.50 21.00 120.5 19.00 19.25 18.00 17.73 18.00 17.92 20.00 19.99 42 12.00 8.00 035.0 1.00 1.00 20.00 220.1 19.00 18.43 17.00 16.97 17.00 16.92 19.00 19.46 43 13.00 7.00 035.0 0.50 0.50 21.00 121.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 44 13.00 9.00 025.0 0.50 0.50 21.00 921.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 45 13.00 9.00 025.0 0.50 1.50 21.00 921.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 0 9 46 12.00 8.00 30.0 0.50 1.00 21.00 21.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 47 13.00 7.00 025.0 1.50 1.50 21.00 921.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 48 11.00 7.00 025.0 1.50 0.50 21.00 921.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 49 12.00 8.00 030.0 1.00 1.00 21.00 921.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 50 13.00 7.00 025.0 1.50 0.50 21.00 921.1 19.00 19.16 18.00 18.15 19.00 19.08 21.00 20.78 0 9 Supplementary Table 2</p><p>S.N Molecular Molecula R.T Area SI Biological activity O Name of the compound formula r weight</p><p>C5H10N2 98 Antibacterial, Anti- 1H-Imidazole, 4,5-dihydro- inflammatory and 1 2,4-dimethyl- 3.25 3003683 97 antifungal</p><p>2 Neoisolongifolene, 8,9-epoxy- C5H22O 218 10.634 1151023 99 Unknown</p><p>3 1-Undecanol C11H24O 172 12.138 242041 99 Flavor, Perfumery</p><p>2- C17H27F7O2 396 Unknown 4 Heptafluorobutyroxytridecane 12.666 164494 99</p><p>Phenol, 2,4-bis(1,1- C17H30OSi 278 Antioxidant 5 dimethylethyl)- 18.704 1012005 99</p><p>1-Dodecanol, 3,7,11- C15H32O 228 Unknown 6 trimethyl- 18.819 133099 98</p><p>7 2-Hexyl-1-octanol C14H30O 214 19.634 105314 99 No activity reported</p><p>C10H22O3S 222 1198500 Unknown 8 Sulfurous acid, dipentyl ester 21.051 7 99</p><p>9 4-Trifluoroacetoxyhexadecane C18H33F3O2 338 23.685 4807929 99 Antimicrobial</p><p>10 1-Decanol, 2-hexyl- C16H34O 242 24.924 169440 99 Unknown</p><p>Butyl-4-amino-1-benzyl C19H28N2O6 380 Unknown piperidine -4-carboxylate, 11 oxalic acid 25.515 3307728 91</p><p>12 3-Octadecene, (E)- C18H36 252 25.687 444075 99 No activity reported</p><p>N-(4-Ethoxy-2-nitrophenyl) C10H12N2O4 174 Unknown 13 acetamide 25.912 4710746 97</p><p>Heptafluorobutanoic acid, C22H37F7O2 466 Unknown 14 octadecyl ester 26.271 93368 98</p><p>N-[4- Cyclooctylaminobutyl] C14H28N2 224 1652309 Unknown 15 aziridine 27.386 4 99</p><p>16 Benzo[c] Phenanthen-5-amine C18H13N 243 27.913 1797968 99 Unknown</p><p>Phthalic acid, ethyl pentyl C15H20 264 Antibacterial 17 ester 28.388 669591 98</p><p>18 L-Alanyl-L-Tryptophan C14H17N3O3 275 29.691 7728888 99 Antimicrobial</p><p>L-Proline, N- C15H25NO4 283 Unknown 19 allyloxycarbonyl-, hexyl ester 30.076 1715933 99</p><p>C13H25NSi 223 1777396 Unknown 20 1-(Triisopropylsilyl)pyrrole 30.555 1 99</p><p>21 Butyric acid, pentadecyl ester C19H38O2 298 31.883 567648 99 Analgesics 22 Isopropyl stearate C21H42O2 326 35.352 617715 98 Unknown  1- CH3(CH2)15S  Antifungal and Hexadecanesulfonyl O2Cl 324 Antioxidant chloride </p><p>23 37.018 148213 99</p><p>24 Diheptyl phthalate C22H34O4 362 43.282 600003 99 Antimicrobial</p><p>25 Dihydrovallesiachotamine C21H24N2O3 352 47.59 113599 89 Unknown</p><p>C32H66 450 Antitubercular, Antioxidant and 26 Dotriacontane 54.472 521116 98 Antispasmodic</p><p>C34H70 478 Antimicrobial and 27 Tetracosane, 11-decyl- 57.38 5285833 99 Antioxidant </p>