Pmcerulean3-Mitochondria-7 Sequence Number: Mito-146

pmCerulean3-Mitochondria-7 Sequence Number: Mito-146

Mitochondrial targeting sequence from subunit VIII of human cytochrome C oxidase

ATGGTGATG - mCerulean3 Sequence (717 Nucleotides - 239 Amino Acids) AGCTGGGGC - mCerulean3 Chromophore (SWG); A206K = AAG TCCGTCCTG - Mitochondrial Targeting Sequence (87 Nucleotides - 29 Amino Acids) LINKER - 7 Amino Acids (21 Nucleotides) between mCerulean3 and MTS ATG - Methionine Start Codon TAG TAA TGA - Stop Codons Antibiotic = Kan/Neo

ACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTC TATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCCGCTAGCATGTCCGTCCTGACGCCGCTGCTGCTGC GGGGCTTGACAGGCTCGGCCCGGCGGCTCCCAGTGCCGCGCGCCAAGATCCATTCGTTGGGGGATCCACCG GTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGG CGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCC TGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGAGCTGGGGC GTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGG CTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCG AGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGG CACAAGCTGGAGTACAACGCCATCCACGGCAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAA GGCCAACTTCGGCCTCAACTGCAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACA CCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCAAGCTGAGCAAA GACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCAT GGACGAGCTGTACAAGTAAAGCGGCCGCGACTCTAGATCATATCAGCCATACCACATTTGTAGAGGTTTAC TTGCTTTAAAAACCTCCCACACTCCCCTGACCTGAA mCerulean3 = mCerulean + T65S/S147H/D148G/K166G/I167L/R168N/H149C

mCerulean3-Mito-7 in HeLa Cells