Appendix 5 to Resolution No. 15630 of Senate of Wroclaw Medical University of 30 March 2016

Syllabus Description of the course

Module/Course Molecular Medicine Techniques – Group of detailed clinical applications education results Group Group name code Scientific basis B, C of medicine

Preclinical Sciences

Faculty English Division

Major Medicine

Specialties Not applicable

Level of studies Uniform magister studies X* 1st degree studies 2nd degree studies  3rd degree studies  postgraduate studies  Form of studies X full-time X part-time

Year of studies III-V Semester  Winter X Summer Type of course  obligatory  limited choice X free choice / elective Course  major X basic

Language of instruction  Polish X English  other

* mark  with an X

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t F y a S s l c M h e a C P s r t s P s i a l l a C i c e p S Winter Semester Appendix 5 to Resolution No. 15630 of Senate of Wroclaw Medical University of 30 March 2016

Summer Semester 20

TOTAL per year:

20

Educational objectives (max. 6 items) C1. Expanding student knowledge about the main trends in genetic and cellular therapy development. C2. Understanding of DNA vaccines operating principles.

C3. Developing the ability to plan and construct expression plasmid vectors.

C4. The pharmacogenetics and personal pharmacotherapy principles understanding. Learning practical skills to detect of SNPs.

C5. Improving knowledge about epigenetics and methods for its analysis. Developing practical skills to analyse gene and microRNA expression. C6. Introduction to cell culture methods. Education result matrix for module/course in relation to verification methods of the intended education result and the type of class Methods of Number of Form of Number of Student who completes the verification of major didactic class course module/course knows/is able to intended education education **enter the education result results (forming and result abbreviation summarising) W01 C.W41 Knows main trends in genetic and Oral response LC cellular therapy development. Project of Is aware of pros and cons of gene expression vector W02 therapy application in patients. preparation into gene therapy W03 Knows how DNA vaccines act. application.

W04 Understands a phenomenon of RNA interference and knows how to use it for gene silencing therapy.

W05 C.W40 Understands principles of pharmacogenetics and personal Appendix 5 to Resolution No. 15630 of Senate of Wroclaw Medical University of 30 March 2016

pharmacotherapy.

W06 C.W9 Describes techniques used for SNP investigation. Knows the principles of epigenetics and methods for DNA methylation analysis. W07 Understands the cloning phenomena and describes all necessary steps for successful completion of this process.

U01 B.U11 Uses databases, including Oral response LC websites, and is able to find necessary information using the Project of expression vector available tools preparation into gene therapy Recognizes genomic and U2 application. complementary sequences of DNA. Evaluation of practical U03 Designs primers for PCR. competency U04 Is able to carry out reactions, which are essential for cloning e.g. PCR, RT, restriction, ligation. U05 Understand the real-time PCR method and knows how to use it for investigation of DNA genotyping ,methylation and mircroRNA expression. Makes all calculations and interprets results.

K 01 Cooperates with group. Evaluation of K02 Actively performs experiments. practical K03 Is responsible for the result of the competency. whole group work. ** L - lecture; SE - seminar; AC – auditorium classes; MC – major classes (non-clinical); CC – clinical classes; LC – laboratory classes; SCM – specialist classes (magister studies); CSC – classes in simulated conditions; FLC – foreign language course; PCP practical classes with patient; PE – physical education (obligatory); VP – vocational practice; SS – self-study, EL – E-learning .

Please mark on scale 1-5 how the above effects place your classes in the following categories: communication of knowledge, skills or forming attitudes: Knowledge: 3 Skills: 5 Appendix 5 to Resolution No. 15630 of Senate of Wroclaw Medical University of 30 March 2016

Social competences: 2

Student's amount of work (balance of ECTS points)

Student's workload Student Workload (h) (class participation, activity, preparation, etc.) 1. Contact hours: 20

2. Student's own work (self-study): 6

Total student's workload 26

ECTS points for module/course 1

Comments

Content of classes (please enter topic words of specific classes divided into their didactic form and remember how it is translated to intended educational effects) Lectures 1. 2. 3.

Seminars 1. 2. 3.

Practical classes 1. Gene therapy- introduction. Application of gene therapy in oncology and cardiovascular disease. 2. Construction of expression vectors. Work with gene bank databases. Design of primers and protocols for PCR. 3. Cellular therapy. Main principles of cell culture. Methods of DNA delivery. Transfection of cells in vitro. 4. Methods of transferred gene detection. 5. Epigenetic regulation of gene expression. RNA interference. Application siRNA and miRNA in gene therapy. Work with miR databases. Real-time PCR principles, analysis of results. 6. Application of DNA vaccines. 7. Pharmacogenetics. Influence of gene mutation on drug metabolism. Analyses of SNP.

Other 1. 2. 3. etc. … Basic literature (list according to importance, no more than 3 items) 1. 1. Herzog R.W., Zolotukhin S. A guide to human gene therapy. World Scientific Publishing Co, Singapure 2010.

2. Lattime E.C, Gerson S.L. Gene therapy of cancer. Elsevier Academic Press, Third edition 2014 Appendix 5 to Resolution No. 15630 of Senate of Wroclaw Medical University of 30 March 2016

3. Barnes L.P. New research on pharmacogenetics. Nova Science Publishers, Inc, New York 2007

Additional literature and other materials (no more than 3 items) Current original and review publications – supplied by teacher Didactic resources requirements (e.g. laboratory, multimedia projector, other…)

Laboratory, cell culture room, laminar chamber, incubator-CO2, fluorescence microscope, multimedia projektor, laptops, thermocycler, real-time thermocycler, centrifuge, thermoblok, UV-transiluminator, mini horizontal electrophoresis chamber.

Preliminary conditions (minimum requirements to be met by the student before starting the module/course) None

Conditions to receive credit for the course (specify the form and conditions of receiving credit for classes included in the module/course, admission terms to final theoretical or practical examination, its form and requirements to be med by the student to pass it and criteria for specific grades) A project preparation about the construction of expression vector for gene therapy.

Positive evaluation of student attitude (activity and oral response)

Grade: Criteria (only for courses/modules ending with an examination) Very Good (5.0) Good Plus (4.5) Good (4.0) Satisfactory Plus (3.5) Satisfactory (3.0)

Name and address of module/course teaching unit, contact: telephone and e-mail address

.Department of Forensic Medicine, Molecular Techinques Unit, ul. Curie-Skłodowskiej 52, 50-369 Wrocław, Phone: (071) 784-15-97, e-mail: [email protected]

.Coordinator / Person responsible for module/course, contact: telephone and e-mail address

Dagmara Baczyńska, PhD

Phone: 717841597 Appendix 5 to Resolution No. 15630 of Senate of Wroclaw Medical University of 30 March 2016 e-mail: [email protected]

List of persons conducting specific classes: full name, degree/scientific or professional title, discipline, performed profession, form of classes.

Dagmara Baczyńska/PhD/molecular biology/laboratory classes

Date of Syllabus development Syllabus developed by 30.06.2016 ………………………………………….. Dagmara Baczyńska.....

Signature of Head of teaching unit

……………....……………………………………………………………… Signature of Faculty Dean

……………....………………………………………………………………