Supplementary Table 1. Classification of Blood Pressure(BP)

Supplementary table 1. Classification of blood pressure(BP)

JNC7 category SBP mmHg DBP mmHg Normal <120 and <80 Prehyertension 120-139 or 80-89 Hypertension

Stage 1 140-159 or 90-99

Stage 2 ≥160 or ≥100 Source: The Seventh Report of the Joint National Committee on Prevention, Detection,

Evaluation, and Treatment of High Blood Pressure. JAMA 2003,289:2560-2571. Supplementary table 2. Genotype distributions of KLKB1 tag SNPs in hypertension patients and controlsa (1317:639)

Tag SNPs Allel Group No. of genotypes (frequency) MA Adjusted OR (95%CI; P-Value)b

es F (1/2) 1/1 1/2 2/2 2 vs 1 1/2+2/2 vs 1/1 rs2278542 C/A Contro 596(0.92 42(0.08 0(0.00) 0.03 1.47(1.00- 1.47(0.99-

l ) ) 3(0.00) 0.05 2.17;0.048) 2.18,0.055)

Case 1189(0.9 115(0.0

1) 9) rs2304595 G/A Contro 260(0.42 301(0.4 78(0.12) 0.36 0.82(0.70- 0.78(0.63-

l ) 6) 133(0.1 0.33 0.95;0.010) 0.96;0.021)

Case 599(0.45 584(0.4 1)

) 4) rs4253325 G/A Contro 328(0.50 257(0.4 53(0.09) 0.28 1.18(1.01- 1.30(1.06-

l ) 1) 116(0.0 0.31 1.39,0.039) 1.61;0.012)

Case 605(0.46 589(0.4 9)

) 5) rs925453 C/T Contro 473(0.73 159(0.2 6(0.01) 0.13 1.12(0.91- 1.05(0.83-

l ) 6) 30(0.02) 0.14 1.39,0.277) 1.33;0.694) Case 961(0.73 320(0.2

) 4) aHypertension was defined as average systolic BP of ≥150 mmHg, an average diastolic BP ≥95 mmHg, or current treatment for hypertension with antihypertensive medication. Control subjects had systolic BP <120 mmHg and diastolic BP <80 mmHg.

The major allele was always referred to as allele 1 and the minor allele as allele 2.

b OR estimated by logistic regression analysis, adjusted for Gender, age, smoking, drinking, BMI, TC, TG, Cr, HDL_C and Glu.

Supplementary table 3. Association between KLK1 gene haplotypes and essential hypertension (1317:639) Frequencies variables Haplotype All Cases Controls OR (95% CI) a P-Value

Hap1 AGAC 0.040 0.044 0.032 1.64(1.54-1.75) 0.000

Hap2b CAGC 0.332 0.320 0.358 reference ——

Hap3 CGAC 0.263 0.268 0.252 1.25(1.04-1.49) 0.016 Hap4 CGGC 0.221 0.220 0.224 1.13(0.93-1.37) 0.207

Hap5 CGGT 0.139 0.142 0.133 1.25(0.99-1.56) 0.057

Hypertension was defined as average systolic BP of ≥150 mmHg, an average diastolic BP ≥95 mmHg, or current treatment for hypertension with antihypertensive medication. Control subjects had systolic BP <120 mmHg and diastolic BP <80 mmHg. aGender, age, smoking, drinking, BMI, TC, TG, Cr, HDL_C and Glu were adjusted.

b Hap2 (CAGC) was chosen to be the baseline haplotype.

2 Supplementary Table 4 R L of 27 remaining SNPs “explained” the 4 tag SNPs

2 Locus region MAF Locus R L rs4253238 5’flanking 0.37 0.91 rs1912826 intron 2 0.37 0.91 rs1511801 intron 2 0.37 0.91 rs1511802 intron 2 0.30 0.86 rs2304596 intron 3 0.21 0.87 rs4253248 intron 3 0.37 0.91 rs4253251 intron 3 0.11 0.68 rs4253252 intron 3 0.37 0.91 rs3733402 exon 5 0.37 0.91 rs4253259 intron 6 0.07 0.22 rs4253260 intron 6 0.21 0.87 rs4253292 intron 6 0.17 0.49 rs6848171 intron 6 0.32 0.95 rs4253299 intron 6 0.11 0.68 rs4253302 intron 11 0.21 0.87 rs4253303 intron 11 0.30 0.86 rs4253304 intron 11 0.30 0.86 rs4253308 intron 11 0.32 0.95 rs4253311 intron 11 0.36 0.95 rs4253315 intron 11 0.31 0.95 rs2292423 intron 11 0.32 0.95 rs4241821 intron 12 0.11 0.68 rs3775302 intron 13 0.34 0.91 rs4861709 intron 13 0.16 1.00 rs4253326 intron 14 0.11 0.68 rs4253327 intron 14 0.43 0.87 rs3087505 3'UTR 0.09 0.53 SNPs are presented in chromosomal order.

2 R L was not calculated for SNPs with MAF<0.05

2 SNPs with R L <0.85 are indicated in bold.