Clinical Chemistry and Laboratory Medicine Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

CCLM is the official journal of the Association of Clinical Biochemists in Ireland (ACBI), the Austrian Society of Laboratory Medicine and Clinical Chemistry (ÖGLMKC), the German Society for Clinical Chemistry and Laboratory Medicine (DGKL), the Hungarian Society of Laboratory Medicine (MLDT), the Royal Belgian Society of Laboratory Medicine (RBSLM), the Italian Society of Clinical Biochemistry and Clinical Molecular Biology (SIBioC), the Portuguese Society of Clinical Chemistry, Genetics and Laboratory Medicine (SPML), the Slovenian Association for Clinical Chemistry and Laboratory Medicine (SZKKLM) and the Spanish Society of Laboratory Medicine (SEQC). CCLM is affiliated with the Australasian Association of Clinical Biochemists (AACB) and Société Francaise de Biologie Clinique (SFBC).

Editor-in-Chief Editorial Board Markus Herrmann Tze Ping Lo Mario Plebani Ivan Brandslund Graz, Italy Singapore Padova, Italy Odense, Denmark Martin Hersberger David Meyre Janne Cadamuro Zurich, Switzerland Hamilton, Canada Yesim Ozarda Associate Editors Salzburg, Austria Johannes J.M.L. Hoffmann Giuseppe Lippi Etienne Cavalier Nuenen, Netherlands Bursa, Turkey Tomris Özben Reviews Editor Liege, Belgium Zhi-De Hu Sail Chun Hohhot, P.R. China Antalya, Turkey Verona, Italy Berend Isermann Vladimir Palicka Philippe Gillery Seoul, Korea Pradeep Kumar Dabla Magdeburg, Germany Hradec Králové, Reims, France Czech Republic New Delhi, India Joannes F.M. Jacobs, Ronda Greaves Mauro Panteghini Vincent De Guire Nijmegen, Netherlands Bundoora, Australia Milan, Italy Montreal, Canada Petr Jarolim Karl J. Lackner Boston, MA, USA Geraldo Picheth Vincent Delatour Graham Jones Curitiba, Brazil Mainz, Germany Paris, France Darlinghurst, NSW, Australia Ana-Maria Simundic Bohuslav Melichar Eleftherios P. Diamandis Olomouc, Czech Republic János Kappelmayer Zagreb, Croatia Toronto, Canada Grazyna Sypniewska Deborah A. Payne Debrecen, Hungary Rajiv T. Erasmus Peter Kavsak Bydgoszcz, Poland Denver, CO, USA Stellenbosch, South Africa Michael Vogeser Peter Schlattmann Toronto, Canada Emmanuel Favaloro Christos Kroupis Munich, Germany Jena, Germany Westmead, Australia Athens, Greece Maria Alice V. Willrich Juan M. Guerrero Leslie Charles Lai Rochester, MN, USA Seville, Spain Kuala Lumpur, Malaysia João Tiago Guimarães Michael Laposata Managing Editor Porto, Portugal Galveston, TX, USA Heike Jahnke Liam Heaney Mark W. Linder Berlin, Germany Loughborough, UK Louisville, KY, USA Abstracted/Indexed in Analytical Abstracts; Baidu Scholar; Biobase; CABI (over 50 subsections); Case; Chemical Abstracts Service (CAS) - CAplus; Chemical Abstracts Service (CAS) - SciFinder; CNKI Scholar (China National Knowledge Infrastructure); CNPIEC; Dimensions; EBSCO (relevant databases); EBSCO Discovery Service; Embase; Gale/Cengage; Genamics JournalSeek; Google Scholar; ICAP Alcohol Information Databases; Japan Science and Technology Agency (JST); J-Gate; Journal Citation Reports/Science Edition; JournalGuide; JournalTOCs; KESLI-NDSL (Korean National Discovery for Science Leaders); Medline; Meta; Microsoft Academic; Naviga (Softweco); Norwegian Register for Scientific Journals, Series and Publishers; Pathway Studio; Primo Central (ExLibris); ProQuest (relevant databases); Publons; PubMed; PubsHub; ReadCube; Reaxys; SCImago (SJR); SCOPUS; Sherpa/RoMEO; Summon (Serials Solutions/ProQuest); TDNet; TEMA Technik und Management; Text Mining; Ulrich's Periodicals Directory/ulrichsweb; WanFang Data; Web of Science - Biological Abstracts; Web of Science - BIOSIS Previews; Web of Science - Current Contents/Life Sciences; Web of Science - Prous Science Integrity; Web of Science - Science Citation Index; Web of Science - Science Citation Index Expanded; WorldCat (OCLC).

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ISSN 1434-6621 ∙ e-ISSN1437-4331 ∙ CODEN CCLMFW

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Cover illustration Research lab © Eisenhans. Permission to use the image on the cover granted by Fotolia LLC. Clin Chem Lab Med 2020 | Volume 58 | Issue 6

Contents

Special Issue: Advancements in Mass Spectrometry as a Tool for Clinical Analysis. Part 2 Issue Editor: Liam Heaney

Editorial Klára Ščupáková, Benjamin Balluff, Caitlin Tressler, Tobi Adelaja, Ron M.A. Heeren, Kristine Glunde and Liam M. Heaney Gökhan Ertaylan Advancements in mass spectrometry as a tool for clinical Cellular resolution in clinical MALDI mass spectrometry analysis: part II 855 imaging: the latest advancements and current challenges 914 Quantitative protein assessment Sophie E. Lellman and Rainer Cramer Bacterial identification by lipid profiling using liquid Dobrin Nedelkov and Yueming Hu atmospheric pressure matrix-assisted laser Complexity, cost, and content – three important factors for desorption/ionization mass spectrometry 930 translation of clinical protein mass spectrometry tests, and the case for apolipoprotein C-III proteoform testing 858

Kendall W. Cradic, Paula M. Ladwig, Ann L. Rivard, Clinical application of ’omics technologies Waddah Katrangi, Karl Florian Wintgens and Maria A.V. Willrich Biswapriya Misra Vedolizumab quantitation using high-resolution Individualized metabolomics: opportunities and accurate mass-mass spectrometry middle-up protein challenges 939 subunit: method validation 864 Diana Canetti, Nigel B. Rendell, Janet A. Gilbertson, Wencan Jiang, Gongwei Sun, Xinyu Wen, Shasha Men, Nicola Botcher, Paola Nocerino, Angel Blanco, Wenbin Cui, Miao Jing, Xingwang Jia, Zhian Hu, Lucia Di Vagno, Dorota Rowczenio, Guglielmo Verona, Danna Pu, Sichun Zhang, Xiaozhou Yuan, Xiaoting Liu, P. Patrizia Mangione, Vittorio Bellotti, Philip N. Hawkins, Xinrong Zhang and Chengbin Wang Julian D. Gillmore and Graham W. Taylor Development and evaluation of an element-tagged Diagnostic amyloid proteomics: experience of the immunoassay coupled with inductively coupled plasma UK National Amyloidosis Centre 948 mass spectrometry detection: can we apply the new assay in the clinical laboratory? 873 Giuseppe Lippi and Liam M. Heaney The “olfactory fingerprint”: can diagnostics be improved by combining canine and digital noses? 958 MALDI-MS for the clinic Daniela Basso, Andrea Padoan, Renata D’Incà, Muhammad Zubair Israr, Dennis Bernieh, Giorgio Arrigoni, Maria Luisa Scapellato, Nicole Contran, Andrea Salzano, Shabana Cassambai, Yoshiyuki Yazaki Cinzia Franchin, Greta Lorenzon, Claudia Mescoli, Stefania and Toru Suzuki Moz, Dania Bozzato, Massimo Rugge and Mario Plebani Matrix-assisted laser desorption ionisation (MALDI) Peptidomic and proteomic analysis of stool for diagnosing mass spectrometry (MS): basics and clinical IBD and deciphering disease pathogenesis 968 applications 883 James A. Ross, Johannes P.C. Vissers, Jyoti Nanda, Sylvia P. Nauta, Martijn Poeze, Ron M.A. Heeren and Grant D. Stewart, Holger Husi, Fouad K. Habib, Tiffany Porta Siegel Dean E. Hammond and Lee A. Gethings Clinical use of mass spectrometry (imaging) for hard The influence of hypoxia on the prostate cancer tissue analysis in abnormal fracture healing 897 proteome 980 Clin Chem Lab Med 2020 | Volume 58 | Issue 6

Laboratory automation and kit-based Sally C. Benton, Godwin K. Tetteh, Sarah-Jayne Needham, approaches Jakob Mücke, Leanne Sheppard, Steven Alderson, Corinne Ruppen, Maurus Curti, Maurice Redondo and Anna M. Milan Gian Luca Salvagno, Elisa Danese and Giuseppe Lippi Evaluation of the 25-hydroxy vitamin D assay on Mass spectrometry and total laboratory automation: a fully automated liquid chromatography mass opportunities and drawbacks 994 spectrometry system, the Thermo Scientific Cascadion SM Clinical Analyzer with the Cascadion Caroline Le Goff, Jordi Farre-Segura, Violeta Stojkovic, 25-hydroxy vitamin D assay in a routine clinical Patrice Dufour, Stéphanie Peeters, Justine Courtois, laboratory 1010 Adrien Nizet, Nathalie De Vos and Etienne Cavalier The pathway through LC-MS method development: in-house or ready-to-use kit-based methods? 1002