Pharmaceutical Evaluation of Western Australian Sandalwood Seed Oil
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School of Pharmacy Pharmaceutical Evaluation of Western Australian Sandalwood Seed Oil Dhanushka Sugeeshwara Hettiarachchi This thesis is presented for the Degree of Doctor of Philosophy of Curtin University August 2014 Declaration To the best of my knowledge and belief this thesis contains no material previously published by any other person except where due acknowledgement has been made. This thesis contains no material which has been accepted for the award of any other degree or diploma in any university. Dhanushka Sugeeshwara Hettiarachchi Signature: ………………………………………….. Date : ..…………………………………………. i Women behind my success: My late grandmothers, my mother and my wife. Man who inspired me: My father ii Best virtues of a gentleman appear while facing hardships, such as a mature log of Sandalwood spreads the aroma upon chopping. - 15th century Sinhala poetry “Subhashitha” by Alagiyawanna Mukaweti iii Acknowledgement I would first give my salutation to Western Australian Sandalwood tree for paving my path for education and rewarding me financially, while scenting my day with much loved aroma. My supervisor Prof. Sunderland, I am in debt for your guidance and encouragement throughout the time of this study. Your knowledge and wisdom is always delivered with usual patience, I am indeed privileged and proud to be your student. I also kindly acknowledge the generous funding you provided towards the collaborative research projects. Dr. Yandi Liu, the person who introduced me to sandalwood industry and also co-supervised this study, I have no words to thank you but owe you a lot for what I am today. Thank you for mentoring me for all these years, and the familial treat your family has provided. It is an honour to be known as a student of Prof. John Fox, thank you very much for accepting to be my associate supervisor. Your advice and guidance have made me a complete sandalwood man. Mr. Michael Boddy, thank you for sharing your knowledge and guiding through all my laboratory work, you also taught me how to conduct laboratory practices, which has enormously helped my professional life. Dr. Rima Caccetta, thank you for your support as my supervisor during the last stage of my study. Your academic and administrative input was invaluable in completing this thesis. I highly appreciate the support from Prof. Jeff Hughes both as my thesis committee chairman and the Head of the School. I acknowledge the Australian Government for providing me the Australian Postgraduate Award and Curtin University for providing me the Curtin Research Scholarship during this study. This thesis and my studies into sandalwood would have never been realised without support of the Wescorp Group. Mr. Tim Coakley, your help in drafting this project and your technical and financial support was generous throughout the study. I thankful for Ms. Vanessa Pereira for proof reading my entire thesis. I thank everyone of the Wescorp family; Mr. Nathan O’Neill, Mr. Peter MacKinnon, Mr. Jay Coakley, Ms. Marie Farrow , Ms. Mirjana Stojic, Ms. Melinda Campbell, Ms. Fleur Coakley, Ms. Annette Siemes, Ms. Winnie Harper, Mr. Norman Butler, Mr. Daniel Donovan, late Mr. Neville Pratt and all the staff at the factory, all your hard work and iv dedication is presented here. Thank you for all the generosity and trust you showed, I enjoy and pride myself at every moment working with you at Wescorp. All the staff members of the School of Pharmacy need a special acknowledgement. I would acknowledge, Dr. Paul Murray, Ms. Kathy Whiteman, Dr. Richard Parsons, Dr. Madhu Page-Sharp, Ms. Joyce Thomas, Mr. Jorge Martinez, Mr. Giuseppe Luna and Assoc. Prof. Lynne Emmerton for your support during this study. I gratefully remember the support received from late Mr. Peter Mioduszewski of Department of Environment and Agriculture, Curtin University. I would like to thank my friend Chirag for sharing your knowledge and all that coffee and food we had over political debates. I acknowledge the support received from Zen Ang during the plantation studies. I would also appreciate Sangeetha, Remya, Vikas, Elsamuel, Dimitri, Svetlana, Dolzenko family and all the friends who were there in time of need. Prof. Duo Li and Department of Food Science, Zhejiang University for hosting me for my exchange study and also the Western Australian government and Zhejiang Provincial government for providing me the scholarship to conduct my exchange study in the beautiful city of Hangzhou, China. I am grateful for my collaborator and good friend Fang Cai and all the friends at Zhejiang University for looking after me and the support given during my stay, a big Xie Xie for all of you. I am thankful for the kind cooperation given by Mr. Gordon Taylor in acquiring seeds for the study. I would like to thank Dr. Hans Wolmuth and Mr. Ashely Dowell from Southern Cross Plant Science for biological analysis conducted on biological studies; also the staff of CiTox laboratory, France for conducting the OECD toxicity analysis. I am grateful to Mr. Richard Daniels and Nutrazeal Ltd., New Zealand for all the support received during pilot studies. I would also express my gratitude to Dr. Upul Subasinghe of Sri Jayewardenepura University, Sri Lanka; who is a good friend and a colleague in sandalwood and agarwood research over the years. I would acknowledge the support and funding from Sadaharitha Plantations Ltd. for collaborative research projects in Sri Lanka. I would like to thank my good friend Mr. Grant Pronk for helping me in plantation studies and all the lessons given on agro-forestry. Also the input from Mr. Jonathan v Brand and Mr. Benjamin Sawyer of Forest Products Commission is highly appreciated. I appreciate the support received from Prof. Vijay Jayasena and Dr. Mala Senarathne of School of Public Health, Curtin University. My friends Chathura, Ashan, Rohitha, Oshadi, Sirikumara family, Rathnasekara family, Liyanage family, Ediriweera family and Fernando family; your presence made easy for me to call Perth home. I like to thank my brother Missaka and sister- in-law Induni. I also thank my father-in-law, mother-in-law and sister-in-law for their relentless support. My parents and both the late grandmothers have sacrificed a lot for the betterment of my life and education. I hope this work would stand a tribute to what you have given to me. This study is a result of dedication, care and understanding of my loving wife Sonia, you have being the driving force for all my life’s missions and thank you for been the greatest pillar of support in achieving this goal. vi Table of Contents Declaration ................................................................................................................... i Acknowledgement ..................................................................................................... iv Table of Contents ..................................................................................................... vii List of Figures ........................................................................................................... xii List of Tables ........................................................................................................... xiv Abstract .................................................................................................................... xvi List of Publications and Presentations .................................................................. xix Abbreviations ........................................................................................................... xx Chapter 1 Introduction ........................................................................................... 1 1.1 Western Australian Sandalwood ...................................................................... 1 1.1.1 Botanical Background ................................................................................. 1 1.1.2 Sandalwood Essential Oil ............................................................................ 5 1.1.3 Sandalwood Industry in Western Australia ................................................. 6 1.1.4 Sandalwood Plantations ............................................................................... 7 1.2 Plant Lipids ...................................................................................................... 9 1.2.1 Lipid Components ....................................................................................... 9 1.2.2 Lipid Analysis ............................................................................................ 10 1.2.3 Plant Lipids in Pharmaceutical and Cosmetic Preparations ...................... 12 1.3 Sandalwood Seed Oil ..................................................................................... 13 1.3.1 Chemical Composition .............................................................................. 13 1.3.2 Ximenynic Acid ......................................................................................... 15 1.3.3 Pharmacological Studies ............................................................................ 18 1.3.4 Ethnobotanical Evidence ........................................................................... 20 1.3.5 Future Applications of Sandalwood Seed Oil ............................................ 21 1.4 Current Study ................................................................................................. 22 Chapter 2 General Methodology ......................................................................... 23 2.1 Materials ........................................................................................................