ISMM NEWSLETTER, Volume 1, Issue 14, Date Released:2020-03-03
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Medicinal Potential of Mycelium and Fruiting Bodies of an Arboreal
www.nature.com/scientificreports OPEN Medicinal potential of mycelium and fruiting bodies of an arboreal mushroom Fomitopsis ofcinalis in therapy of lifestyle diseases Agata Fijałkowska1, Bożena Muszyńska1*, Katarzyna Sułkowska‑Ziaja1, Katarzyna Kała1, Anna Pawlik2, Dawid Stefaniuk2, Anna Matuszewska2, Kamil Piska3, Elżbieta Pękala3, Piotr Kaczmarczyk4, Jacek Piętka5 & Magdalena Jaszek2 Fomitopsis ofcinalis is a medicinal mushroom used in traditional European eighteenth and nineteenth century folk medicine. Fruiting bodies of F. ofcinalis were collected from the natural environment of Świętokrzyskie Province with the consent of the General Director for Environmental Protection in Warsaw. Mycelial cultures were obtained from fragments of F. ofcinalis fruiting bodies. The taxonomic position of the mushroom mycelium was confrmed using the PCR method. The presence of organic compounds was determined by HPLC–DAD analysis. Bioelements were determined by AF‑AAS. The biochemical composition of the tested mushroom material was confrmed with the FTIR method. Antioxidant properties were determined using the DPPH method, and the antiproliferative activity was assessed with the use of the MTT test. The presence of indole compounds (l‑tryptophan, 6‑methyl‑d,l‑tryptophan, melatonin, 5‑hydroxy‑l‑tryptophan), phenolic compounds (p‑hydroxybenzoic acid, gallic acid, catechin, phenylalanine), and sterols (ergosterol, ergosterol peroxide) as well as trace elements was confrmed in the mycelium and fruiting bodies of F. ofcinalis. Importantly, a high level of 5‑hydroxy‑l‑tryptophan in in vitro mycelium cultures (517.99 mg/100 g d.w) was recorded for the frst time. The tested mushroom extracts also showed antioxidant and antiproliferative efects on the A549 lung cancer cell line, the DU145 prostate cancer cell line, and the A375 melanoma cell line. -
Piptoporus Betulinus, Fomitopsis Betulina)
Birch Fungi – Razor Strop, Birch Bracket (Piptoporus betulinus, Fomitopsis betulina) Features - This distinctive fungus only grows on birches, looks like nothing else that grows on birches, and is very common. It is not an aggressive tree killer, but is instead primarily in the business of decomposing dead trees. Birch polypore is present throughout the range of the birches, which grow around the globe in the northern hemisphere. The white-to-brownish fruiting bodies are annual, emerging from the bark of birches in spring and summer, but they deteriorate slowly and are still visible through the winter, though by then they have blackened and are not so attractive. Global Uses – Otzi the Iceman, who lived 5300 years ago, carried two fragments of a fruiting body of Fomitopsis betulina (formerly Piptoporus betulinus). Some scientists believe that Ӧtzi might have used the fungus for medical purposes and, although the idea arouses some controversy, the long tradition of the use of F. betulina in folk medicine is a fact. Infusion from F. betulina fruiting bodies was popular, especially in Russia, Baltic countries, Hungary, Romania for its nutritional and calming properties. Fungal tea was used against various cancer types, as an immunoenhancing, anti-parasitic agent, and a remedy for gastrointestinal disorders. Antiseptic and anti-bleeding dressings made from fresh F. betulina fruiting body were applied to wounds and the powder obtained from dried ones was used as a painkiller. Was used as a razor strop to sharpen fine edged blades. Medicinal Potential – Chemical Constituents: A and C, 1,3-beta-D-glucopyranan, B ergosta-7,22-dien-3- ol, fungisterol, ergosterol, agaric acid, dehydrotumulosic acid, ungalinic acid, betulinic acid, and tumulosic acid. -
Polypore Diversity in North America with an Annotated Checklist
Mycol Progress (2016) 15:771–790 DOI 10.1007/s11557-016-1207-7 ORIGINAL ARTICLE Polypore diversity in North America with an annotated checklist Li-Wei Zhou1 & Karen K. Nakasone2 & Harold H. Burdsall Jr.2 & James Ginns3 & Josef Vlasák4 & Otto Miettinen5 & Viacheslav Spirin5 & Tuomo Niemelä 5 & Hai-Sheng Yuan1 & Shuang-Hui He6 & Bao-Kai Cui6 & Jia-Hui Xing6 & Yu-Cheng Dai6 Received: 20 May 2016 /Accepted: 9 June 2016 /Published online: 30 June 2016 # German Mycological Society and Springer-Verlag Berlin Heidelberg 2016 Abstract Profound changes to the taxonomy and classifica- 11 orders, while six other species from three genera have tion of polypores have occurred since the advent of molecular uncertain taxonomic position at the order level. Three orders, phylogenetics in the 1990s. The last major monograph of viz. Polyporales, Hymenochaetales and Russulales, accom- North American polypores was published by Gilbertson and modate most of polypore species (93.7 %) and genera Ryvarden in 1986–1987. In the intervening 30 years, new (88.8 %). We hope that this updated checklist will inspire species, new combinations, and new records of polypores future studies in the polypore mycota of North America and were reported from North America. As a result, an updated contribute to the diversity and systematics of polypores checklist of North American polypores is needed to reflect the worldwide. polypore diversity in there. We recognize 492 species of polypores from 146 genera in North America. Of these, 232 Keywords Basidiomycota . Phylogeny . Taxonomy . species are unchanged from Gilbertson and Ryvarden’smono- Wood-decaying fungus graph, and 175 species required name or authority changes. -
New Data on Aphyllophoroid Fungi (Basidiomycota) in Forest-Steppe Introduction Communities of the Lipetsk Region, European Russia
Acta Mycologica DOI: 10.5586/am.1112 ORIGINAL RESEARCH PAPER Publication history Received: 2018-06-05 Accepted: 2018-09-30 New data on aphyllophoroid fungi Published: 2018-12-28 (Basidiomycota) in forest-steppe Handling editor Anna Kujawa, Institute for Agricultural and Forest communities of the Lipetsk region, Environment, Polish Academy of Sciences, Poland European Russia Authors’ contributions SV designed the research, 1 2 1 collected and identifed the Sergey Volobuev *, Alexandra Arzhenenko , Sergey Bolshakov , material; AA collected and Nataliya Shakhova3, Lyudmila Sarycheva4 identifed the material; SB 1 Laboratory of Systematics and Geography of Fungi, Komarov Botanical Institute, Russian identifed the material; NS and Academy of Sciences, Professor Popov Str. 2, St. Petersburg 197376, Russia LS collected the material; all 2 Botany Chair, Biological Department, Saint Petersburg State University, Universitetskaya authors contributed to the Embankment 7–9 Str., Petersburg 199034, Russia manuscript preparation 3 Laboratory of Biochemistry of Fungi, Komarov Botanical Institute, Russian Academy of Sciences, Professor Popov Str. 2, St. Petersburg 197376, Russia Funding 4 Laboratory of Mycology, Galichya Gora Nature Reserve, Voronezh State University, Donskoye The work was supported by the village, Zadonsky District, Lipetsk region 399240, Russia program of basic research of the Russian Academy of Sciences, * Corresponding author. Email: [email protected] project “Biological diversity and dynamics of the fora and vegetation of Russia”, using equipment of the Core Facility Abstract Center of the Komarov Botanical Te data on 150 species of aphyllophoroid fungi from the Lipetsk region, Central Institute “Cell and Molecular Russian Upland, European Russia, are presented. Te annotated species list based Technologies in Plant Science”. -
ISMM NEWSLETTER, Volume 1, Issue 8, Date Released:2017-12-18
Volume 1, Issue 8 Date-released: December 18, 2017 News reports - The 9th International Medicinal Mushrooms Conference (IMMC9) - The 11th Chinese Mushroom Festival held in Zhangzhou Up-coming events - First Circular of the First Chinese (Gutian) Rare Mushroom Conference - Welcome to International Mycological Congress (IMC) 11 Research progress - New Researches - Recommendation of Book--Edible and Medicinal Mushrooms Technology and Applications, Edited by Diego Cunha Zied and Arturo Pardo-Gimenez Points and Reviews - Medicinal Mushrooms (Part III), by Jure Pohleven, Tamara Korošec, Andrej Gregori - Medicinal Mushrooms in Human Clinical Studies. Part I. Anticancer, Oncoimmunological, and Immunomodulatory Activities: A Review (Part I), by Solomon P. Wasser Call for Papers Contact information Issue Editor- Mr. Ziqiang Liu [email protected] Department of Edible Mushrooms, CFNA, 4/F, Talent International Building No. 80 Guangqumennei Street, Dongcheng District, Beijing 10062, China News Reports The 9th International Medicinal Mushrooms Conference (IMMC9), September 24-28, 2017, Palermo, Italy Maria Letizia Gargano1& Giuseppe Venturella2 1Department of Earth and Maine Science, University of Palermo, Bld. 16, I-90128 Palermo (Italy); 2Department of Agricultural, Food and Forest Sciences, University of Palermo, Bld. 5, I-90128 Palermo (Italy) In September 2017 over 200 delegates from 49 different countries (Fig. 1) gathered in Splendid Hotel La Torre, Mondello (Palermo, Italy), for the 9th International Medicinal Mushrooms Conference. IMMC9 in Palermo was the first to be held in Italy. The theme to the Conference was “Advances in Medicinal Mushroom Science: Building Bridges between Western and Eastern Medicine”. IMMC9 participants had the opportunity to discuss and share scientific innovations in the medicinal mushroom sector and to become aware of current research results. -
Ethnomycological Investigation in Serbia: Astonishing Realm of Mycomedicines and Mycofood
Journal of Fungi Article Ethnomycological Investigation in Serbia: Astonishing Realm of Mycomedicines and Mycofood Jelena Živkovi´c 1 , Marija Ivanov 2 , Dejan Stojkovi´c 2,* and Jasmina Glamoˇclija 2 1 Institute for Medicinal Plants Research “Dr Josif Pancic”, Tadeuša Koš´cuška1, 11000 Belgrade, Serbia; [email protected] 2 Department of Plant Physiology, Institute for Biological Research “Siniša Stankovi´c”—NationalInstitute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11000 Belgrade, Serbia; [email protected] (M.I.); [email protected] (J.G.) * Correspondence: [email protected]; Tel.: +381-112078419 Abstract: This study aims to fill the gaps in ethnomycological knowledge in Serbia by identifying various fungal species that have been used due to their medicinal or nutritional properties. Eth- nomycological information was gathered using semi-structured interviews with participants from different mycological associations in Serbia. A total of 62 participants were involved in this study. Eighty-five species belonging to 28 families were identified. All of the reported fungal species were pointed out as edible, and only 15 of them were declared as medicinal. The family Boletaceae was represented by the highest number of species, followed by Russulaceae, Agaricaceae and Polypo- raceae. We also performed detailed analysis of the literature in order to provide scientific evidence for the recorded medicinal use of fungi in Serbia. The male participants reported a higher level of ethnomycological knowledge compared to women, whereas the highest number of used fungi species was mentioned by participants within the age group of 61–80 years. In addition to preserving Citation: Živkovi´c,J.; Ivanov, M.; ethnomycological knowledge in Serbia, this study can present a good starting point for further Stojkovi´c,D.; Glamoˇclija,J. -
Chemical Composition and Biological Activity of Extracts from Fruiting Bodies and Mycelial Cultures of Fomitopsis Betulina
Molecular Biology Reports https://doi.org/10.1007/s11033-018-4420-4 ORIGINAL ARTICLE Chemical composition and biological activity of extracts from fruiting bodies and mycelial cultures of Fomitopsis betulina Katarzyna Sułkowska‑Ziaja1 · Agnieszka Szewczyk1 · Agnieszka Galanty2 · Joanna Gdula‑Argasińska3 · Bożena Muszyńska1 Received: 25 May 2018 / Accepted: 4 October 2018 © The Author(s) 2018 Abstract Fomitopsis betulina (Bull.) B.K. Cui, M.L. Han & Y.C. Dai has been used for medicinal purposes for over 5000 years. Numerous studies have confirmed the biological activity of compounds found in this species. The purpose of this study was a comparative analysis of selected groups of metabolites in the extracts from fruiting bodies and mycelial cultures. Phenolic acids (syringic, gallic, p-hydroxybenzoic, 3,4-dihydrophenylacetic), indole compounds (L-tryptophan, 5-hydroxy-L-tryp- tophan, 5-methyltryptamine), sterols (ergosterol, ergosterol peroxide, hexestrol, cholecalciferol), and triterpenes (betulinic acid, betulin) were determined quantitatively by high performance liquid chromatography with UV–Vis/DAD detection, while fatty acids were assessed with the gas chromatography method. Cytotoxic activity against selected human cancer cell lines was determined using the lactate dehydrogenase test. Anti-inflammatory activity was evaluated on lipopolysaccharide activated A549 cells. Those extracts with anti-inflammatory activity were evaluated for their inhibition of pro-inflammatory enzymes. The mycelium extract exhibited significant cytotoxic activity against prostate cancer cells, while the fruiting body extract indicated a moderate effect on the viability of melanoma and prostate cancer. Incubation of lung epithelial cells with biomass extract significantly decreased cyclooxygenase-2 levels compared to LPS activated A549 cells. This paper is the first report of a comparative quantitative analysis of the metabolites in mycelial cultures and fruiting bodies. -
José E. Sánchez, Gerardo Mata and Daniel J. Royse Editors
Updates on Tropical Mushrooms. Basic and Applied Research José E. Sánchez, Gerardo Mata and Daniel J. Royse Editors EE 635.8 U6 Updates on Tropical Mushrooms. Basic and applied research / José E. Sánchez, Gerardo Mata and Daniel J. Royse, editors.- San Cristóbal de Las Casas, Chiapas, México: El Colegio de la Frontera Sur, 2018. 227 p. : photographs, illustrations, maps, portraits; 22.7X17 cm. ISBN: 978-607-8429-60-8 It includes bibliography 1. Tropical mushrooms, 2. Agaricus, 3. Edible mushrooms, 4. Medicinal mushrooms, 5. Mushroom cultivation, 6. Biotechnology, 7 Mexico, 8. China, 9. Guatemala, 10 Cuba, I. Sánchez, José E. (editor), II. Mata Gerardo (editor). III. Royse, Daniel J. (editor). 1st. Edition, 2018 The content of this book was subjected to a process of blind peer reviewing according to rules established by the Editorial Committee of El Colegio de la Frontera Sur. DR © El Colegio de la Frontera Sur www.ecosur.mx El Colegio de la Frontera Sur Carretera Panamericana y Periférico Sur s/n Barrio de María Auxiliadora CP 29290 San Cristóbal de Las Casas, Chiapas, México Photographies of the front page: clockwise, Agaricus subrufescens (D.C. Zied), Agaricus martinicensis (C. Angelini),. Lentinula boryana (G. Mata), Lepista nuda (M.C. Bran), Agaricus trisulphuratus (P. Callac), Sparassis latifolia (Lu Ma), L. boryana (G. Mata), S. latifolia (Lu Ma). At the center, A. subrufescens (G. Mata) No part of this book may be reproduced or transmitted in any form by any means without written permission from the editors. Printed and made in Mexico Acknowledgments Printing of this book was supported financially by Fondos Mixtos Conacyt through the Project FOMIX-13149 “Design, construction, equipment and startup of a state center for innovation and technology transfer for the development of coffee growing in Chiapas, Mexico” and through the Project MT-11063 of Ecosur “Social and environmental innovation in coffee growing areas for reducing vulnerability”. -
South Yorkshire Branch Newsletter Winter 2018-19 Editor Pauline Rutherford M.B.N.A
South Yorkshire Branch Newsletter Winter 2018-19 Editor Pauline Rutherford M.B.N.A. Issue number 25 Jack Frost at work! Photo by P. Rutherford View from a Comfy Chair By The Chairman First of all, thank you to all who sent messages of support and congratulations at the news of my new position of Hon. Chairman of the BNA. A position that makes me feel both proud and humble in equal measures. Following Roger, and before him Professor Cloudsley-Thompson, who between them have held the post since the late 1970’s, are huge shoes to try to fill. And, even though this will mean me looking more outwards to the rest of the British Isles, my heart will always be in South Yorkshire. As for this edition of our Newsletter, I think that it could have been subtitled “Seeing the wood for the trees”; as you know, Pauline and I have been spending the winter chicken sitting in Suffolk very close to the Wildlife Trust reserve of Knettleshall, and I find Catherine’s article where she tells us that “wood pasture could be the origin of several habitat types such as heath, moorland, bog and fen” very accurate when I look at the recovery of the heath at Knettleshall. One of my favourite trees here is in a field just off the reserve and is a true veteran English oak that could be a thousand years old! And with seven articles written by seven members, covering so much of the natural world I am proud to be surrounded by such knowledgeable people, well done to you all. -
Carbon and Oxygen Gas Exchange in Woody Debris: the Process and Climate-Related Drivers
Article Carbon and Oxygen Gas Exchange in Woody Debris: The Process and Climate-Related Drivers Victor A. Mukhin 1,2,* , Daria K. Diyarova 1 , Mikhail L. Gitarskiy 3,4 and Dmitry G. Zamolodchikov 5 1 Institute of Plant and Animal Ecology, Ural Division of the Russian Academy of Sciences, 620144 Yekaterinburg, Russia; [email protected] 2 Institute of Natural Sciences and Mathematics, Ural Federal University, 620026 Yekaterinburg, Russia 3 Ecology and Environmental Management Program, Ugresha Branch, Dubna State University, 140090 Dzerzhinsky, Russia; [email protected] 4 Russian Energy Agency, Ministry of Energy of the Russian Federation, 129085 Moscow, Russia 5 Center for Forest Ecology and Productivity, Russian Academy of Sciences, 117234 Moscow, Russia; [email protected] * Correspondence: [email protected]; Tel.: +7-(343)-210-38-53 Abstract: The carbon-to-oxygen relationship and gas exchange balance, organic carbon to CO2 conversion intensity and efficiency, and their relevance to climate parameters and wood decay fungi were investigated for birch woody debris (WD) in the Mid-Urals mixed pine and birch forests. It was shown that, within the range of temperatures from 10 to 40 ◦C and relative moisture (RM) of wood of 40% and 70%, aerobic gas exchange was observed in the WD, encompassing the physiologically entwined processes of CO2 emission and O2 uptake. Their volumetric ratio (0.9) confirmed that (1) the WD represents a globally significant CO2 source and appropriate O2 consumer and (2) the oxidative conversion of organic carbon is highly efficient in the WD, with an average ratio of CO 2 released to O2 consumed equal to 90%. -
Traditional Medicinal Plants in South Tyrol (Northern Italy, Southern Alps): Biodiversity and Use Joshua Petelka1, Barbara Plagg2,3, Ina Säumel4* and Stefan Zerbe1
Petelka et al. Journal of Ethnobiology and Ethnomedicine (2020) 16:74 https://doi.org/10.1186/s13002-020-00419-8 REVIEW Open Access Traditional medicinal plants in South Tyrol (northern Italy, southern Alps): biodiversity and use Joshua Petelka1, Barbara Plagg2,3, Ina Säumel4* and Stefan Zerbe1 Abstract Background: Worldwide mountain regions are recognized as hotspots of ethnopharmacologically relevant species diversity. In South Tyrol (Southern Alps, Italy), and due to the region’s high plant diversity and isolated population, a unique traditional botanical knowledge of medicinal plants has flourished, which traces its history back to prehistoric times. However, changes in rural life and culture may threaten this unique biodiversity and cultural heritage. Our study aims to collect and analyze information on native plants used in traditional folk medicine, focusing on the preservation of botanical and cultural diversity. Methods: Data were collected through a review of published material that documents traditionally used medicinal plants of South Tyrol in order to capture the total diversity of plants and their usage. We evaluated different parameters, comprising the ethnobotanicity index (EI), ethnophytonomic index (EPI), relative frequency of citation (RFC), red list status, and regional legislation with regard to the plant species. Results: A total of 276 species, including 3 mushrooms and 3 lichens, were identified. These belonged to 72 families, most frequently to the Asteraceae, Rosaceae, and Lamiaceae. The most frequently cited species were Hypericum perforatum L., Urtica dioica L., and Plantago lanceolata L. According to 12 ICPC-2 disease categories, the most frequently treated human health symptoms were from the digestive and respiratory systems as well as the skin. -
Investigating Wood Decaying Fungi Diversity in Central Siberia, Russia Using ITS Sequence Analysis and Interaction with Host Trees
sustainability Article Investigating Wood Decaying Fungi Diversity in Central Siberia, Russia Using ITS Sequence Analysis and Interaction with Host Trees Ji-Hyun Park 1, Igor N. Pavlov 2,3 , Min-Ji Kim 4, Myung Soo Park 1, Seung-Yoon Oh 5 , Ki Hyeong Park 1, Jonathan J. Fong 6 and Young Woon Lim 1,* 1 School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, Korea; [email protected] (J.-H.P.); [email protected] (M.S.P.); [email protected] (K.H.P.) 2 Laboratory of Reforestation, Mycology and Plant Pathology, V. N. Sukachev Institute of Forest, Siberian Branch of Russian Academy of Sciences, 660036 Krasnoyarsk, Russia; [email protected] 3 Department of Chemical Technology of Wood and Biotechnology, Reshetnev Siberian State University of Science and Technology, 660049 Krasnoyarsk, Russia 4 Wood Utilization Division, Forest Products Department, National Institute of Forest Science, Seoul 02455, Korea; [email protected] 5 Department of Biology and Chemistry, Changwon National University, Changwon 51140, Korea; [email protected] 6 Science Unit, Lingnan University, Tuen Mun, Hong Kong; [email protected] * Correspondence: [email protected]; Tel.: +82-2880-6708 Received: 27 February 2020; Accepted: 18 March 2020; Published: 24 March 2020 Abstract: Wood-decay fungi (WDF) play a significant role in recycling nutrients, using enzymatic and mechanical processes to degrade wood. Designated as a biodiversity hot spot, Central Siberia is a geographically important region for understanding the spatial distribution and the evolutionary processes shaping biodiversity. There have been several studies of WDF diversity in Central Siberia, but identification of species was based on morphological characteristics, lacking detailed descriptions and molecular data.