2004–2005 I Am Delighted to Present This My First Annual Report As Director of the Mcdonald Institute
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The Polymerase Chain Reaction (PCR): the Second Generation of DNA Analysis Methods Takes the Stand, 9 Santa Clara High Tech
Santa Clara High Technology Law Journal Volume 9 | Issue 1 Article 8 January 1993 The olP ymerase Chain Reaction (PCR): The Second Generation of DNA Analysis Methods Takes the Stand Kamrin T. MacKnight Follow this and additional works at: http://digitalcommons.law.scu.edu/chtlj Part of the Law Commons Recommended Citation Kamrin T. MacKnight, The Polymerase Chain Reaction (PCR): The Second Generation of DNA Analysis Methods Takes the Stand, 9 Santa Clara High Tech. L.J. 287 (1993). Available at: http://digitalcommons.law.scu.edu/chtlj/vol9/iss1/8 This Comment is brought to you for free and open access by the Journals at Santa Clara Law Digital Commons. It has been accepted for inclusion in Santa Clara High Technology Law Journal by an authorized administrator of Santa Clara Law Digital Commons. For more information, please contact [email protected]. THE POLYMERASE CHAIN REACTION (PCR): THE SECOND GENERATION OF DNA ANALYSIS METHODS TAKES THE STAND Kamrin T. MacKnightt TABLE OF CONTENTS INTRODUCTION ........................................... 288 BASIC GENETICS AND DNA REPLICATION ................. 289 FORENSIC DNA ANALYSIS ................................ 292 Direct Sequencing ....................................... 293 Restriction FragmentLength Polymorphism (RFLP) ...... 294 Introduction .......................................... 294 Technology ........................................... 296 Polymerase Chain Reaction (PCR) ....................... 300 H istory ............................................... 300 Technology .......................................... -
Mittelalterliche Migrationen Als Gegenstand Der "Genetic History"
Jörg Feuchter Mittelalterliche Migrationen als Gegenstand der ‚Genetic History‘ Zusammenfassung Genetic History untersucht historische Fragen mit der Quelle DNA. Migrationen sind ihr Hauptgegenstand, woraus sich eine große Bedeutung für die Mediävistik ergibt. Doch bis vor kurzem waren Historiker nicht beteiligt. Der Beitrag gibt eine Definition der neuen Dis- ziplin (), untersucht die Rolle des Konzeptes Migration in der Populationsgenetik () und beschreibt den Stand der Migrationsforschung in der Mediävistik (). Anschließend gibt er einen kurzen Überblick über die von der Genetic History untersuchten Migrationsräu- me (), betrachtet exemplarisch zunächst eine Studie zur angelsächsischen Migration nach Britannien () und dann ein aktuelles Projekt, in dem erstmals ein Mediävist die Leitung innehat (). Der Beitrag endet mit einigen allgemeinen Beobachtungen und Postulaten (). Keywords: Genetik; DNA; Mittelalter; Angelsachsen; Langobarden; Völkerwanderung. Genetic History is the study of historical questions with DNA as a source. Migrations are its main subject. It is thus very relevant to Medieval Studies. Yet until recently historians have not been involved. The contribution provides a definition of the new discipline (), explores the role of migration as a concept in Population Genetics () and describes the state of migration studies in Medieval History (). It then sets out for an overview of medieval migratory areas studied by Genetic History () and takes an exemplary look first () at a study on Anglo-Saxon migration to Britain and later () at a current project where for the first time a medieval historian has taken the lead. The contribution ends () with some general observations and stipulations. Keywords: Genetics; DNA; Middle Ages; Anglo-Saxons; Lombards; Migration Period. Danksagung: Der Verfasser dankt Prof. Dr. Veronika Lipphardt (University College Frei- burg) und der von ihr von bis geleiteten Nachwuchsgruppe Twentieth Century Felix Wiedemann, Kerstin P. -
Final Programme
Ancient DNA: applications in human evolutionary history Organised by Professor Erika Hagelberg, Professor Michael Hofreiter and Professor Christine Keyser Wednesday 20 - Thursday 21 November 2013 DAY 1 DAY 2 SESSION 1 SESSION 2 SESSION 3 SESSION 4 Human migration Chair Erika Hagelberg Historical transformations Technical issues Human evolution debates 09.00 Welcome by Royal Society Chair Anders Götherström Chair Michael Hofreiter Chair Paul Mellars & lead organiser Agnar Helgason Paula Campos Eva-Maria Geigl Carles Lalueza-Fox Assessing the authenticity of Pleistocene humans in the Palaeogenomic analysis of Paleogenomics and the 09.05 13.30 clonal sequence reads from 09.00 13.30 Americans/East Asia and their ancient humans: Mesolithic-Neolithic transition ancient samples: a case study environment methodological issues in Europe from Iceland 09.30 Discussion 14.00 Discussion 09.30 Discussion 14.00 Discussion Pontus Skoglund David Reich Anne Stone Genomic analysis of prehistoric Erika Hagelberg The landscape of Neandertal 09.45 Impacts of colonization in the 14.15 populations in Europe: 09.45 Shall we still worry about 14.15 ancestry in present-day humans Americas authentication and population contamination? history 10.15 Discussion 14.45 Discussion 10.15 Discussion 14.45 Discussion 10.30 Coffee 15.00 Tea 10.30 Coffee 15.00 Tea Marie-France Deguilloux Concepción de la Rua Odille Loreille Where are the Caribs? Ancient The Neolithic transition in the Elena Gigli Next generation sequencing 11.00 DNA from ceramic period 15.30 Cantabrian fringe -
Pleistocene Island Occupation in the Mediterranean: Insights from a Tied-Biome Approach to Glacial Refugia
Pleistocene Island Occupation in the Mediterranean: Insights from a Tied-Biome Approach to Glacial Refugia Phoca-Cosmetatou, N., & Rabett, R. J. (2014). Pleistocene Island Occupation in the Mediterranean: Insights from a Tied-Biome Approach to Glacial Refugia. In K. Boyle, R. J. Rabett, & C. O. Hunt (Eds.), Living in the Landscape: Essays in Honour of Graeme Barker (pp. 83-108). (McDonald Institute Monograph Series). McDonald Institute for Archaeological Research. http://www.mcdonald.cam.ac.uk/publications/publication- images/table%20of%20contents/LivingintheLandscape.pdf/view Published in: Living in the Landscape: Essays in Honour of Graeme Barker Document Version: Publisher's PDF, also known as Version of record Queen's University Belfast - Research Portal: Link to publication record in Queen's University Belfast Research Portal Publisher rights © 2014 McDonald Institute for Archaeological Research General rights Copyright for the publications made accessible via the Queen's University Belfast Research Portal is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Take down policy The Research Portal is Queen's institutional repository that provides access to Queen's research output. Every effort has been made to ensure that content in the Research Portal does not infringe any person's rights, or applicable UK laws. If you discover content in the Research Portal that you believe breaches copyright or violates any law, please contact [email protected]. Download date:25. Sep. 2021 McDONALD INSTITUTE MONOGRAPHS Living in the Landscape Essays in Honour of Graeme Barker Edited by Katherine Boyle, Ryan J. -
Profile of Alec J. Jeffreys
Profile of Alec J. Jeffreys s one of the great contributors to modern genetics, Sir Alec Jeffreys was born with curiosity in his genes as the son and Agrandson of prolific inventors. Jeffreys displayed an insatiable quest for knowl- edge, and his father fostered his son’s budding scientific interests with gifts of a microscope and chemistry set, the latter of which produced one of Jeffreys’ most memorable scientific ventures. ‘‘Those were back in the happy days of chemis- try,’’ Jeffreys points out, ‘‘where you could go down to your local pharmacist and get virtually everything you wanted.’’ The end result of that chemistry experiment was the detonation of his aunt’s apple tree and a set of scars that Jeffreys still bears to- day. ‘‘You learn science very fast that way,’’ he says, ‘‘but it was quite fun.’’ Jeffreys’ scientific curiosity only in- creased after the apple tree incident, and years later it would lead to one of the Alec J. Jeffreys most widely used applications in genetics: DNA fingerprinting. Among other uses, the DNA fingerprinting technique has freys go there, so Jeffreys entered the turn led to another groundbreaking find- helped solve numerous criminal investi- university in 1968. He was at first intent ing about the composition of eukaryotic gations, settle countless paternity dis- on pursuing a degree in biochemistry DNA: introns (3). ‘‘I was 27 at the time, putes, and spark a resurgence of interest but soon decided to alter his course. so still a real rookie, with the ability to in the forensic sciences. That achieve- ‘‘This was no criticism of the way it was detect single-copy DNA by Southern blot ment alone is worthy of merit, contrib- taught,’’ he says of biochemistry at Ox- hybridization and a nice paper on introns uting to Jeffreys’ receiving three high ford, ‘‘but rather a reflection of the fac- under my belt, which is, yeah, not a bad distinctions in 2005: the Albert Lasker ulty’s research, which leaned heavily to start,’’ he says. -
Jones 06.Pdf
Far from the Hearth (Above) Martin Jones at West Stow, 1972 (with thanks to Ian Alister, Lucy Walker, Leonie Walker, and West Stow Environmental Archaeology Group); (Below) Martin Jones in a millet field, Inner Mongolia, 2010. (Photograph: X. Liu.) McDONALD INSTITUTE CONVERSATIONS Far from the Hearth Essays in Honour of Martin K. Jones Edited by Emma Lightfoot, Xinyi Liu & Dorian Q Fuller Published by: McDonald Institute for Archaeological Research University of Cambridge Downing Street Cambridge CB2 3ER UK (0)(1223) 339327 [email protected] www.mcdonald.cam.ac.uk McDonald Institute for Archaeological Research, 2018 © 2018 McDonald Institute for Archaeological Research Far from the Hearth is made available under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 (International) Licence: https://creativecommons.org/licenses/by-nc-nd/4.0/ ISBN: 978-1-902937-87-8 Cover image: Foxtail millet field near Xinglonggou, Chifeng, China, photographed by Xinyi Liu, September 2014. Edited for the Institute by James Barrett (Series Editor) and Anne Chippindale. Contents Contributors vii Figures viii Tables xvi Acknowledgements xx Foreword xxi James H. Barrett Part I Introduction 1 Introduction: Far from the Hearth 3 Xinyi Liu, Emma Lightfoot & Dorian Q Fuller Part II A Botanical Battleground 7 Chapter 1 The Making of the Botanical Battle Ground: Domestication and the Origins of the Worlds’ Weed Floras 9 Dorian Q Fuller & Chris J. Stevens Chapter 2 The Fighting Flora: An Examination of the Origins and Changing Composition of the Weed Flora of the British Isles 23 Chris J. Stevens & Dorian Q Fuller Chapter 3 A System for Determining Plant Macro Archaeological Remains 37 Victor Paz Chapter 4 Phytoliths and the Human Past: Archaeology, Ethnoarchaeology and Paleoenvironmental Studies 51 Carla Lancelotti & Marco Madella Chapter 5 Genetics and the Origins of European Agriculture 65 Terry Brown Chapter 6 Martin Jones’ Role in the Development of Biomolecular Archaeology 71 Terry Brown, Richard P. -
Living in the Landscape Essays in Honour of Graeme Barker
McDONALD INSTITUTE MONOGRAPHS Living in the Landscape Essays in Honour of Graeme Barker Edited by Katherine Boyle, Ryan J. Rabett & Chris O. Hunt Published by: McDonald Institute for Archaeological Research University of Cambridge Downing Street Cambridge, UK CB2 3ER (0)(1223) 333538 (0)(1223) 339336 (Production Office) (0)(1223) 333536 (FAX) [email protected] www.mcdonald.cam.ac.uk Distributed by Oxbow Books United Kingdom: Oxbow Books, 10 Hythe Bridge Street, Oxford, OX1 2EW, UK. Tel: (0)(1865) 241249; Fax: (0)(1865) 794449; www.oxbowbooks.com USA: Casemate Academic, P.O. Box 511, Oakville, CT 06779, USA. Tel: 860-945-9329; Fax: 860-945-9468 ISBN: 978-1-902937-73-1 ISSN: 1363-1349 (McDonald Institute) © 2014 McDonald Institute for Archaeological Research All rights reserved. No parts of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without the prior permission of the McDonald Institute for Archaeological Research. Cover design by Dora Kemp. Cover image: An archway within Gasr ash-Shahdiyn, a Late Roman fortress located on the main road between Al-Baida and Al-Maj, 4.5 km southwest of the Wadi Kuf bridge, Libya. (Photograph: Ryan Rabett, 2008; details: Paul Bennett). Edited for the Institute by James Barrett (Series Editor) and Anne Chippindale. Printed and bound by Short Run Press, Bittern Rd, Sowton Industrial Estate, Exeter, EX2 7LW, UK. Contents Contributors vii Figures x Tables xii Acknowledgements xv Foreword xvi James Barrett Part I Introduction 1 Chapter 1 Introduction: Frameworks and Landscapes 3 Colin Renfrew Part II Before the Holocene 9 Chapter 2 Hallam Movius, Helmut de Terra and the Line that Never Was: Burma, 1938 11 Robin Dennell Chapter 3 First Modern Human Occupation of Europe: The Middle Danube Region as a Case Study 35 Philip R. -
The DNA Fingerprinting Story Mark a Jobling
Jobling Investigative Genetics 2013, 4:20 http://www.investigativegenetics.com/content/4/1/20 OPINION Open Access Curiosity in the genes: the DNA fingerprinting story Mark A Jobling It is unusual for a scientific field to be associated with a The first DNA fingerprinting application was in par- single individual, but in the case of the subject of the the- entage testing [6]; normally it is the father who is in matic series now being launched in Investigative Genetics, doubt, but this unusual and challenging case was a ma- this is surely so; Alec Jeffreys (Figure 1) is DNA finger- ternity test, with paternal DNA unavailable. British nurse printing. Having invented the method, he coined the per- Christiana Sarbah’s 13-year-old son Andrew was denied fect name for it - how different things might have been if re-entry to the United Kingdom after a visit to Ghana, it had been called the tandem-repeat-based identification the immigration authorities suspecting that he was not technique (or something similarly dull). He realized its her child. Given three undisputed children for comparison, potential and immediately applied, developed and refined it was possible to reconstruct the absent father’sDNA it. He then followed his nose to unravel the mystery of fingerprint, and to strongly support the claimed maternity the madly mutable minisatellites that make up DNA over alternative relationships such as aunt-nephew - some- fingerprints, and eventually to understand the engines of thing that was not achieved with traditional protein poly- genome variability that reside in recombination hotspots. morphisms such as blood groups. -
Past Human Migrations in East Asia: Matching Archaeology, Linguistics
16 A genetic perspective on the origins and dispersal of the Austronesians Mitochondrial DNA variation from Madagascar to Easter Island Erika Hagelberg, Murray Cox, Wulf Schiefenhövel, and Ian Frame Introduction This chapter describes the study of one genetic system, mitochondrial DNA (mtDNA), within the Austronesian-speaking world, and discusses whether the patterns of genetic variation at this genetic locus are consistent with the principal models of the settlement and linguistic diversity in this vast geographical region. Mitochondria are the seat of cellular metabolism and have their own complement of DNA. MtDNA is a tiny proportion of the genetic makeup of a human being, and has both advantages and disadvantages in population studies. It is inherited maternally, in contrast with the nuclear chromosomal DNA inherited from both parents. (Note: the mitochondria of the spermatozoon are eliminated from the egg soon after fertilization, so offspring do not inherit paternal mtDNA, although there are exceptions to this rule, as shown by Schwartz and Vissing 2002.) The maternal mode of inheritance permits the study of female lineages through time. Despite the small size of the mitochondrial genome, each mitochondrion has many thousands of copies of mtDNA, and there are many mitochondria in each cell. The abundance of mtDNA in cells has been exploited in anthropological research on ancient, rare or degraded biological samples, including archaeological bones, old hair samples, or dried blood (Hagelberg 1994). MtDNA accumulates mutations at a relatively fast rate, and so is useful for the investigation of recent evolutionary events. These features have made mtDNA an excellent marker for geneticists interested in human evolutionary history (see Wallace 1995 for review). -
A Lecture by Professor Erika Hagelberg
H-Announce Is there such a thing as a Jewish genome? A lecture by Professor Erika Hagelberg Announcement published by Leah Sidebotham on Friday, April 1, 2016 Type: Lecture Date: April 12, 2016 Location: United Kingdom Subject Fields: Research and Methodology, Jewish History / Studies, Holocaust, Genocide, and Memory Studies, History of Science, Medicine, and Technology, Contemporary History New developments in DNA technology are having a huge impact on industry, medical science and forensic identification. They have also opened new opportunities for research in archaeology and human evolution, as we have seen in the recent identification of the skeleton of Richard III, and the advances in Neanderthal genetics. The technology is also aiding a growing industry in genetic genealogies, where human identity is defined in terms of patterns of DNA variants, obtained easily and cheaply from commercial companies, and giving rise to controversial notions, like that of a Jewish genome. In this talk Professor Hagelberg will describe the opportunities offered by human evolutionary genetics, as well as some of the limitations and more troubling aspects of research on genetic genealogies. Erika Hagelberg’s father came to the UK on a Kindertransport in 1939, aged 13. She studied biochemistry in London and gained a PhD at Cambridge. Her main field of research is the analysis of DNA from archaeological bones, and she has worked on several high-profile cases, including the identification of the skeletal remains of Joseph Mengele and of the Romanov family. She is professor of evolutionary biology at the University of Oslo, and Cheney Fellow in the Arts at the University of Leeds. -
Encounters, Excavations and Argosies: Essays for Richard Hodges Argosies Essays for Richard Hodges
Encounters, Blurb Moreland Mitchell Leal (eds) Excavations and Encounters, Excavations and Argosies: Essays for Richard Hodges Richard for Essays and Argosies: Excavations Encounters, Argosies Essays for Richard Hodges edited by John Moreland, John Mitchell and Bea Leal Archaeopress Archaeology www.archaeopress.com Archaeopress Moreland cover.indd 1 14/08/2017 15:04:56 Encounters, Excavations and Argosies Essays for Richard Hodges edited by John Mitchell, John Moreland and Bea Leal Archaeopress Archaeology Archaeopress Publishing Ltd Gordon House 276 Banbury Road Oxford OX2 7ED www.archaeopress.com ISBN 978 1 78491 681 7 ISBN 978 1 78491 682 4 (e-Pdf) © Archaeopress and the authors 2017 Cover illustration: Two eagles, dado in the crypt of Abbot Epyphanius, San Vincenzo al Volturno, c. 820 (Photo: Sarah Cocke) All rights reserved. No part of this book may be reproduced, in any form or by any means, electronic, mechanical, photocopying or otherwise, without the prior written permission of the copyright owners. Printed in England by Oxuniprint, Oxford This book is available direct from Archaeopress or from our website www.archaeopress.com Bone plaque with hunting dog leaping over eye. Butrint, Triconch Palace, c. AD 400. (© Butrint Foundation) Contents Contributors �������������������������������������������������������������������������������������������������������������������������������������������������������������������iii Introduction �������������������������������������������������������������������������������������������������������������������������������������������������������������������� -
DNA and the Dead Sea Scrolls
Putting the Pieces Together: DNA and the Dead Sea Scrolls Scott R. Woodward A number of questions concerning the origin and production of the Dead Sea Scrolls may be addressed using DNA analysis. These documents were for the most part written on what is thought to be goat- or sheepskin parchment. Based on radiocarbon and other analyses, these manuscripts date between the mid–second century BCE and the rst century CE.1 Under most conditions it would be remarkable that organic material, like parchment, would survive intact after such a long period of time. However, some of the material is remarkably well preserved because of the unique climate and storage conditions at Qumran. Because these parchments were produced from animal skins, they may contain remnant DNA molecules. Within the last decade, new techniques in molecular biology have been developed that have made it possible to recover DNA from ancient sources. The molecular analysis of ancient DNA (aDNA) from the Judean desert parchment fragments would enable us to establish a genetic signature unique for each manuscript. The precision of the DNA analysis will allow us to identify the species, population, and individual animal from which the parchment was produced. Background The ability to recover biomolecules, most importantly DNA, from ancient remains has opened new research that has many implications.2 Access to aDNA provides the opportunity to study the genetic material of past organisms and identify individual and population histories. Unfortunately, the DNA recovered from archaeological specimens is of such a degraded nature that the usual techniques associated with DNA ngerprinting cannot be used.