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(11) EP 2 307 389 B1

(12) EUROPEAN PATENT SPECIFICATION

(45) Date of publication and mention (51) Int Cl.: of the grant of the patent: C07D 281/16 (2006.01) A61K 31/554 (2006.01) 23.01.2013 Bulletin 2013/04 A61P 25/18 (2006.01) A61P 25/24 (2006.01) A61P 25/22 (2006.01) (21) Application number: 09766953.5 (86) International application number: (22) Date of filing: 18.06.2009 PCT/SE2009/050763

(87) International publication number: WO 2009/154563 (23.12.2009 Gazette 2009/52)

(54) DIBENZOTHIAZEPINE DERIVATIVE AND USE THEREOF DIBENZOTHIAZEPINDERIVAT UND ANWENDUNG DAVON DÉRIVÉ DE DIBENZOTHIAZÉPINE ET SON UTILISATION

(84) Designated Contracting States: • PIERSON JR., M. Edward AT BE BG CH CY CZ DE DK EE ES FI FR GB GR Delaware 19850-5437 (US) HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL • SHENVI, Ashokkumar Bhikkappa PT RO SE SI SK TR Delaware 19850-5437 (US) Designated Extension States: • WESOLOWSKI, Steven AL BA RS Waltham, MA 02451 (US) • WIDZOWSKI, Dan (30) Priority: 20.06.2008 US 74417 P Waltham, MA 02451 (US) • WOOD, Michael (43) Date of publication of application: DE 19850-5437 (US) 13.04.2011 Bulletin 2011/15 (74) Representative: Steinrud, Henrik et al (73) Proprietor: AstraZeneca AB AstraZeneca AB 151 85 Södertälje (SE) Global Intellectual Property S-151 85 Södertälje (SE) (72) Inventors: • BROWN, Dean (56) References cited: Waltham, MA 02451 (US) EP-A1- 0 447 857 EP-A1- 0 447 857 • DAMEWOOD, James R. WO-A1-96/18621 WO-A1-2006/073360 Delaware 19850-5437 (US) WO-A1-2007/053618 WO-A2-2005/063254 • EDWARDS, Phil WO-A2-2005/063254 WO-A2-2006/107948 Delaware 19850-5437 (US) CH-A- 422 793 FR-A- 1 508 536 • HULSIZER, James GB-A- 980 853 RU-A- 2006 126 720 Wilmington, Delaware 19850-5437 (US) SU-A- 457 220 US-A- 3 539 573 • MUIR, James Campbell US-A- 3 962 248 US-A1- 2006 063 754 Macclesfield Cheshire SK10 2NA (GB) US-A1- 2006 063 754 US-A1- 2006 217 366

Note: Within nine months of the publication of the mention of the grant of the European patent in the European Patent Bulletin, any person may give notice to the European Patent Office of opposition to that patent, in accordance with the Implementing Regulations. Notice of opposition shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention). EP 2 307 389 B1

Printed by Jouve, 75001 PARIS (FR) EP 2 307 389 B1

Description

Field of the Invention

5 [0001] The present invention relates to a compound suitable for use in treating bipolar disorders, mood disorders, anxiety disorders, and schizophrenia and other psychotic disorders, pharmaceutically acceptable salts thereof, phar- maceutical compositions comprising the said compound and processes for preparing said compound.

Background of the Invention 10 [0002] A number of drugs have been approved to treat bipolar disorder and schizophrenia (e.g., and atypical ), and treatment of mania has been achieved with several atypical antipsychotics (e.g., risperidone, and ). Other compounds have been used for the clinical treatment of major depressive disorder (e.g., reboxetine and desimpramine) as well as bipolar depression (quetiapine). However, improvement in therapy is 15 still desired in terms of achieving better remission rates, more effective treatment of depression and an improved side- effect profile (e.g., reduced sedation and reduced weight gain). [0003] For nearly 50 years, since the early 1960s, scientific and clinical investigators have sought to understand the pharmacology of neuroleptic compounds. Numerous US and Foreign patents and scientific publications have described hundreds of different tricyclic compounds with varying and anti-depressive properties. In the 20 1960s, CH422793 a Swiss patent filed in 1961, and NL293210 a Dutch patent application filed in 1963, described tricyclic compounds. Almost forty years later, a scientific paper published in 2001 (Behavioral Approach to Nondyskinetic An- tagonists: Identification of Seroquel, J. Med. Chem. 2001, 44, 372-380) described other different tricyclic compounds. Still more recently in early 2009, US Patents 7,491,715 B1 and 7,517,871 B1 were granted describing new analogues of . 25 [0004] Despite having a wide spectrum of pharmacological activity, current bipolar and schizophrenia drugs exhibit variable efficacy and side-effect profiles. Although some current drugs have acute efficacy, remission rates are still low. Safety and tolerability are still issues since approximately 75% of patients experience side effects, and treatment com- pliance is a major issue. Additionally, the mechanism of action of atypical antipsychotics is not well understood, for example, the label of Seroquel states: 30 "the mechanism of action of Seroquel, as with other drugs having efficacy in the treatment of schizophrenia and acute manic episodes associated with bipolar disorder, is unknown. However, it has been proposed that this drug’s efficacy in schizophrenia is mediated through a combination of dopamine type 2 (D2) and serotonin type 2 (5HT2) antagonism. Antagonism at receptors other than dopamine and 5HT2 with similar receptor affinities may explain 35 some of the other effects of Seroquel. Seroquel’s antagonism of histamine H1 receptors may explain the somnolence observed with this drug. Seroquel’s antagonism of adrenergic al receptors may explain the orthostatic hypotension observed with this drug."

Similarly, the label for olanzapine states: 40 "The mechanism of action of olanzapine, as with other drugs having efficacy in schizophrenia, is unknown. However, it has been proposed that this drug’s efficacy in schizophrenia is mediated through a combination of dopamine and serotonin type 2 (5HT2) antagonism. The mechanism of action of olanzapine in the treatment of acute manic episodes associated with Bipolar I Disorder is unknown. 45 Antagonism at receptors other than dopamine and 5HT2 with similar receptor affinities may explain some of the other therapeutic and side effects of olanzapine. Olanzapine’s antagonism of muscarinic M1-5 receptors may explain its anticholinergic effects. Olanzapine’s antagonism of histamine H1 receptors may explain the somnolence observed with this drug. Olanzapine’s antagonism of adrenergic α1 receptors may explain the orthostatic hypotension observed with this drug." 50 [0005] Thus, notwithstanding that numerous tricyclic compounds having antipsychotic and anti-depressant activity have been described and used in therapy, improved ways to treat schizophrenia and bipolar disease are still sought. Particularly, effective treatment of the depressive phase of bipolar disorder is desired and still sought, as is treatment of the manic phase, mood stabilization and maintenance for sufferers of bipolar disorder. 55 [0006] EP447857 describes novel polycyclic compounds for the treatment of an allergic disease or bronchial asthma comprising the step of administering the same. US2006/0063754 describes compounds useful in methods of modulating sleep. FR1508536 describes11-tertiary-aminodibenz[b,f][1,4]oxazepines and thiazepines with central nervous system activity. US3539573describes 11-basic substituted dibenzodiazepines and dibenzothiazepines having activity as neu-

2 EP 2 307 389 B1

roplegics, neuroleptics, neuroleptic , antiemetics, analgesics, sedatives, parasympathicolytics and an- tihistaminics.

Description of the invention: 5 [0007] We contemplate a new target product efficacy profile that is consistent with the putative mechanisms of action of quetiapine in bipolar disorder ( i.e. potent NET inhibition and moderate D2 antagonism). Clinical studies with quetiapine suggest that NET and D2 occupancy in the range of 30 to 60% may be sufficient to drive a therapeutic effect in bipolar disorder. Further, improved safety may be achieved by having less interaction with other targets ( e.g., H1, M1) at clinical 10 doses achieving 50% NET occupancy. [0008] A therapeutic agent with such a profile is expected to provide advantages for the treatment of the depressive phase of bipolar disorder, to have potential for mood stabilization and maintenance of sufferers of bipolar disorder with potential for use in the amelioration of mania associated with bipolar conditions. [0009] Thus, in one aspect, it is desirable to obtain at least one of the following attributes: moderate D2 antagonism; 15 potent NET inhibition; or an H1 in vitro binding Ki value that is close to the value of the K i for NET. In a particular aspect it is desirable to obtain at least one of the following in vitro attributes: D2 GTP γS IC50 less than about 600 nM; D2 binding Ki less than about 200 nM; NET inhibition Ki less than about 50 nM; or H1 that has a Ki in the range of about at least half the value of the Ki for NET to greater than the K i for NET. [0010] In another aspect, it is desirable to identify a compound having potent inhibition of the norepinephrine transporter 20 (NET), moderate D2 receptor antagonism and reduced affinity at secondary targets (e.g., H1, M1) relative to NET. [0011] In a more particular aspect, it is desirable to identify a compound with potent NET inhibition (uptake Ki < 50 nM) moderate D2 antagonist potency (GTPγS IC50 <500 nM) and H1 Ki equivalent to or less than NET Ki. [0012] We have identified and herein describe a compound, fluoro-2- 11-piperazin-1-yl-dibenzo[b,f][1,4]thiazepine, that has not been previously described, having the desirable properties described above. 25 [0013] Thus, a pharmacologically active compound 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine, pharma- ceutically acceptable salts thereof, compositions containing the compound or a pharmaceutically acceptable salt thereof and its use in treating bipolar disorder and schizophrenia with the compound or a pharmaceutically acceptable salt thereof are described herein. [0014] Thus, provided herein is a compound of Formula I: 30

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or a pharmaceutically acceptable salt thereof, wherein Z is H. [0015] Also provided herein is at least one composition comprising a compound of Formula I, or a pharmaceutically 45 acceptable salt thereof. In some embodiments, the compositions comprise a pharmaceutically acceptable carrier, diluent or excipient. [0016] Further described herein are treatments for psychiatric disorders comprising administering to a mammal a therapeutically effective amount of a compound of Formula I, or a pharmaceutically acceptable salt thereof. Particularly, described hereinare treatmentsfor psychiatric disorderscomprising administering toa mammal a therapeuticallyeffective 50 amount of a compound of Formula II

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10

or a pharmaceutically acceptable salt thereof. [0017] Yet further described herein are treatments for bipolar disorder, mood disorders, schizophrenia and other psychotic disorders, or anxiety disorders, comprising administering to a subject a therapeutically effective amount of a 15 compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof. Particularly, described herein are treatments for bipolar disorder, a mood disorder, schizophrenia and other psychotic disorders, or anxiety disorder, comprising administering to a subject a therapeutically effective amount of a compound of Formula II, or a pharmaceu- tically acceptable salt thereof [0018] Still yet further provided herein is a compound of Formula I or Formula II, or a pharmaceutically acceptable salt 20 thereof, for use in treating schizophrenia and other psychotic disorders, an anxiety disorder, and/or a mood disorder. [0019] Additionally provided herein is a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, for use in the manufacture of a medicament for the treatment of schizophrenia and other psychotic disorders, bipolar disorder, an anxiety disorder, and/or a mood disorder. [0020] Still yet additionally provided herein are processes for preparing the compound of Formula I or Formula II, and 25 pharmaceutically acceptable salts thereof; intermediates useful for the preparation of the compound and processes for preparing and using such intermediates.

Detailed Description of Embodiments

30 [0021] Provided herein is the compound of Formula I:

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and pharmaceutically acceptable salts thereof, wherein Z is H. [0022] Specifically provided herein is a compound of Formula I wherein Z is H of Formula II: 45

50

55

referred to herein as 2- fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine, and pharmaceutically acceptable salts there- of.

4 EP 2 307 389 B1

[0023] The compound disclosed herein can be prepared by processes described herein by those skilled in the art of organic synthesis. The compound can be synthesized using the methods described herein, using synthetic methods known in the art of synthetic organic chemistry, or variations thereon as appreciated by those skilled in the art. The starting materials and precursors used in the processes described herein are either commercially available, or readily 5 prepared by established organic synthesis methods, or as described herein. It will be understood by those skilled in the art of organic synthesis that the functionality present on various portions of the molecule must be compatible with the reagents and reactions proposed. Such restrictions to the substituents that are compatible with the reaction conditions will be readily apparent to those skilled in the art and alternate methods should then be used. [0024] The definitions set forth in this application are intended to clarify terms used throughout this application. The 10 term "herein" means the entire application. [0025] As used herein, "pharmaceutically acceptable" refers to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio. 15 [0026] As used herein, "pharmaceutically acceptable salts" refer to derivatives of the disclosed compounds wherein the parent compound is associated with an acidic or basic counter ion. For example, pharmaceutically acceptable salts include those derived from mineral acids such as, for example: hydrochloric acid, nitric acid, phosphoric acid, sulfuric acid, hydroiodic acid, nitrous acid, and phosphorous acid. Pharmaceutically acceptable salts may also be developed with organic acids including aliphatic mono dicarboxylates and aromatic acids. Other pharmaceutically acceptable salts 20 include hydrochloride, sulfate, pyrosulfate, bisulfate, bisulfite, nitrate, phosphate, acetic, glycolic, lactic, pyruvic, malon ic, succinic, glutaric, fumaric, malic, tartaric, citric, ascorbic, maleic, hydroxymaleic, benzoic, hydroxybenzoic, phenylacetic, cinnamic, salicylic, 2-phenoxybenzoic, p-toluenesulfonic acid and other sulfonic acids such as methanesulfonic acid and 2-hydroxyethanesulfonic acid. [0027] The compound may exist in a number of tautomeric forms and references to compounds include all such forms. 25 For the avoidance of doubt, where the compound can exist in one of several tautomeric forms and only one is specifically described or shown, all others are nevertheless embraced by the scope of this invention. [0028] Compounds of the invention may include hydrates and solvates. It will also be understood that certain com- pounds of the present invention may exist in solvated, for example hydrated, as well as unsolvated forms. It will further be understood that the present invention encompasses all such solvated forms of the compounds of the invention. 30 [0029] Compositions are intended to include the formulation of the active component or a pharmaceutically acceptable salt with a pharmaceutically acceptable carrier, and optionally other ingredients. For preparing pharmaceutical compo- sitions, inert, pharmaceutically acceptable carriers can be either solid or liquid. For example, compositions can be formulated by means known in the art into the form of, for example, tablets, capsules, aqueous or oily solutions, sus- pensions, emulsions, creams, ointments, gels, nasal sprays, suppositories, finely divided powders or aerosols or neb- 35 ulisers for inhalation, and for parenteral use (including intravenous, intramuscular or infusion) sterile aqueous or oily solutions or suspensions or sterile emulsions. [0030] Liquid form compositions include solutions, suspensions, and emulsions. Sterile water or water- propylene glycol solutions of the active compounds may be mentioned as an example of liquid preparations suitable for parenteral administration. Liquid compositions can also be formulated in solution in aqueous polyethylene glycol solution. Aqueous 40 solutions for oral administration can be prepared by dissolving the active component in water and adding suitable colorants, flavoring agents, stabilizers, and thickening agents as desired. Aqueous suspensions for oral use can be made by dispersing the finely divided active component in water together with a viscous material such as natural synthetic gums, resins, methylcellulose, sodium carboxymethyl cellulose, and other suspending agents known to the pharmaceu- tical formulation art. 45 [0031] Solid form compositions include powders, tablets, dispersible granules, capsules, cachets, and suppositories. A solid carrier can be one or more substances that may also act as diluents, flavoring agents, solubilizers, lubricants, suspending agents, binders, or tablet disintegrating agents; it can also be an encapsulating material. [0032] In powders, the carrier is a finely divided solid, which is in a mixture with the finely divided active component. In tablets, the active component is mixed with the carrier having the necessary binding properties in suitable proportions 50 and compacted in the shape and size desired. [0033] For preparing suppository compositions, a low- melting wax such as a mixture of fatty acid glycerides and cocoa butter is first melted and the active ingredient is dispersed therein by, for example, stirring. The molten homogeneous mixture is then poured into convenient sized molds and allowed to cool and solidify. [0034] Suitable carriers are magnesium carbonate, magnesium stearate, talc, lactose, sugar, pectin, dextrin, starch, 55 tragacanth, methyl cellulose, sodium carboxymethyl cellulose, a low-melting wax, cocoa butter, and the like. [0035] The term composition is also intended to include the formulation of the active component with encapsulating material as a carrier providing a capsule in which the active component (with or without other carriers) is surrounded by a carrier which is, thus, in association with it. Similarly, cachets are included.

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[0036] The pharmaceutical compositions can be in unit dosage form. In such form, the composition is divided into unit doses containing appropriate quantities of the active component. The unit dosage form can be a packaged preparation, the package containing discrete quantities of the preparations, for example, packeted tablets, capsules, and powders in vials or ampoules. The unit dosage form can also be a capsule, cachet, or tablet itself, or it can be the appropriate 5 number of any of these packaged forms. Methods of preparing dosage forms are disclosed in, for example, Remington’s Pharmaceutical Sciences, Mack Publishing Company, Easton, Pennsylvania, 15th Edition, 1975. [0037] Compositions may be formulated for any suitable route and means of administration. Pharmaceutically accept- able carriers or diluents include those used in formulations suitable for oral, rectal, nasal, topical (including buccal and sublingual), vaginal or parenteral (including subcutaneous, intramuscular, intravenous, intradermal, intrathecal, epidural, 10 intraperitoneally, intrathoracically, intracerebroventricularly, and by injection into the joints) administration. The formu- lations may conveniently be presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. [0038] A particular amount of a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, can be administered in an amount that ranges from about 0.25 mg/kg to about 10 mg/kg. More particularly, it is contem- 15 plated that a patient may be treated with about 0.25 mg/kg to about 5 mg/kg of 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f] [1,4]thiazepine or a pharmaceutically acceptable salt thereof. Most particularly, it is contemplated that a patient suffering with bipolar disorder may be treated with about 0.25 mg/kg to about 0.5 mg/kg of 2- fluoro-11-(piperazin-1-yl)dibenzo[b, f][1,4]thiazepine or a pharmaceutically acceptable salt thereof. Still more particularly it is contemplated that a patient suffering with a depressive phase of bipolar disorder may be treated with a lower amount in the range of about 0.25 20 mg/kg to about 0.5 mg/kg of 2- fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine or a pharmaceutically acceptable salt thereof whereas a patient suffering with a manic phase of bipolar disorder may be treated with a higher amount in the range of about 0.25 mg/kg to about 0.5 mg/kg of 2- fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine or a pharmaceu- tically acceptable salt thereof. [0039] The amount of active ingredient that is combined with one or more excipients to produce a single dosage form 25 will necessarily vary depending upon the host treated and the particular route of administration. The size of the thera- peutically effective dose for therapeutic or prophylactic purposes of the active compound (s) will naturally vary from the guidance provided herein according to the nature and severity of the symptoms or conditions, the age and sex of the animal or patient and the route of administration, according to well known principles of medicine. A clinician may readily determine the effective amount by using numerous methods already known in the art and all such effective amounts 30 are contemplated as being within the scope of the present invention. [0040] Further described herein are treatments, comprising the administration of an effective amount of a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, to a mammal, afflicted with at least one symptom or condition associated with a Psychiatric Disorder. In some embodiments, the Pyschiatric Disorder includes: 1) Anxiety Disorders including Panic Disorder Without Agoraphobia, Panic Disorder With Agoraphobia, Agoraphobia Without History 35 of Panic Disorder, Specific Phobia, Social Phobia, Obsessive-Compulsive Disorder, Post-traumatic Stress Disorder, Acute Stress Disorder, Generalized Anxiety Disorder and Generalized Anxiety Disorder Due to a General Medical Condition; 2) Mood Disorders including a) Depressive Disorders including Major Depressive Disorder and Dysthymic Disorder, and b) Bipolar Depression and/or Bipolar mania including Bipolar I Disorder including those with manic, de- pressive or mixed episodes, and Bipolar II Disorder, c) Cyclothymic Disorder, and d) Mood Disorder Due to a General 40 Medical Condition; and 3) Schizophrenia and other Psychotic Disorders including Psychotic Disorder, Schizophreniform Disorder, Schizoaffective Disorder, Delusional Disorder, Brief Psychotic Disorder, Shared Psychotic Disorder, and Psy- chotic Disorder Due to a General Medical Condition, Dementia and other Cognitive Disorders. Examples of definitions of the above conditions and disorders can be found, for example, in the American Psychiatric Association: Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, Text Revision, Washington, DC, American Psychiatric As- 45 sociation, 2000, herein referred to as "DSM-IV". [0041] Particularly, described herein are treatments for, Bipolar I Disorder including those with depressive, manic, or mixed episodes, and Bipolar II Disorder, Cyclothymic Disorder, and Mood Disorder Due to a General Medical Condition; and Schizophrenia comprising administering a therapeutically effective amount of a compound of Formula I, or a phar- maceutically acceptable salt thereof to a patient in need thereof. Most particularly, is provided treatments for Bipolar 50 Disorders including those with depressive, manic, or mixed episodes, comprising administering a therapeutically effective amount of a compound of Formula I wherein Z is H, or a pharmaceutically acceptable salt thereof to a patient in need thereof. [0042] In some embodiments, the symptoms and conditions include anxiety, agitation, hostility, panic, an eating dis- order, an affective symptom, a mood symptom, a negative and positive psychotic symptom commonly associated with 55 psychosis and neurodegenerative disorders. [0043] The term "treating" within the context of the present invention encompasses the administration of a therapeu- tically effective amount of a compound of the present invention to mitigate or inhibit either a pre-existing disease state, acute or chronic, or a recurring symptom or condition. Also encompassed are prophylactic therapies for prevention of

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recurring conditions and continued therapy for chronic disorders. [0044] The term "mammal" refers to any warm- blooded animal, such as a human. In some embodiments, the mammal is in need of treatment because it is suffering from or prone to developing one or more of the symptoms, diseases, or disorders mentioned herein. 5 [0045] The term "administering" includes administering the pharmaceutically active ingredient or a pro-drug thereof, which may convert upon administration to the pharmaceutically active ingredient. [0046] One expected benefit of administering 2- fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine or a pharmaceu- tically acceptable salt thereof is the lesser incidence of at least one potential side effect such as, for example, somnolence, sedation, a cardiovascular side effect, or a side effect associated with D2 antagonists (e.g., movement disorders). 10 [0047] Thus, it is contemplated that treatment with 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine or a phar- maceutically acceptablesalt thereof, willprovide beneficialimprovement by providing acompound havingpotent inhibition of the norepinephrine transporter (NET), moderate D2 receptor antagonism and reduced affinity at secondary targets (e.g., H1 or M1) relative to NET for treating Bipolar and related Mood conditions classified in DSM-IV codes 296 and the subdivisions thereof. Correspondingly, it is contemplated that 2- fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine 15 or a pharmaceutically acceptable salt thereof, will be useful for treating Bipolar and related Mood conditions however such conditions are classified in the future in DSM- V. [0048] It is also contemplated that treatment with 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine or a pharma- ceutically acceptable salt thereof, will be useful for treating Anxiety conditions classified in DSM-IV codes 300 and the subdivisions thereof. Correspondingly, it is contemplated that treatment with 2- fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4] 20 thiazepine or a pharmaceutically acceptable salt thereof, will be useful for treating Anxiety conditions however such conditions are classified in the future in DSM-V. [0049] It is further contemplated that treatment with 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine or a phar- maceutically acceptable salt thereof, will be useful for treating Schizophrenic conditions classified in DSM- IV codes 295 and the subdivisions thereof. Correspondingly, it is contemplated thattreatment with 2- fluoro-11-(piperazin-1-yl)dibenzo 25 [b,f][1,4]thiazepine or a pharmaceutically acceptable salt thereof, will be useful for treating Schizophrenic conditions however such conditions are classified in the future in DSM- V.

Intermediate Compounds:

30 [0050] Intermediate compounds of Formula (A), useful in the synthesis of compounds of Formula I or Formula II, are described in the examples and schemes herein.

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5 6 5 wherein R is NH2 or NO2 and R is H or C1-4alkyl. In a more particular embodiment of a compound of Formula A, R 6 is NH2 and R is H, methyl or ethyl. [0051] Still other intermediates of Formula (B), useful in the synthesis of compounds of Formula I or Formula II, are 45 described in the examples and schemes herein.

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wherein X is Cl or phenoxy.

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[0052] In order that the invention disclosed herein may be more efficiently understood, examples are provided below.

EXAMPLES

5 Example 1A: 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine

[0053] Scheme A shows one method of preparing 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine (VI).

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[0054] Thus, in a five-step process: 5-fluoro-2-mercapto-benzoic acid ethyl ester may be reacted with fluoro-1- 2- nitrobenzene to form 5- fluoro-2-(2-nitro-phenylsulfanyl)-benzoic acid ethyl ester; the ethyl ester may be converted to 5- fluoro-2-(2-amino-phenylsulfanyl)-benzoic acid ethyl ester; the aminophenyl compound may be cyclized to form 2- fluoro- 30 10H-dibenzo[b,f][1,4]thiazepin-11-one, that may be converted to 11- chloro-2-fluoro-dibenzo[b,f][1,4]thiazepine, and that may be then reacted with piperazine to form the title compound.

Step 1:

35 [0055] To a solution of ethyl 5- fluoro-2-mercaptobenzoate (I) (25.0 g, 124.9 mmol) and 1-fluoro-2-nitrobenzene (13.2

mL, 124.9 mmol) in acetone (700 mL) was added 2KCO3 (34.5 g, 249.7 mmol) at ambient temperature. The yellow suspension was heated to reflux (60 °C) for 5 hours. The reaction mixture was quenched with 1N HCl (500 mL), diluted with EtOAc (1000 mL) and filtered through a bed of diatomaceous earth. The aqueous layer was removed, the EtOAc layer was washed with 1N HCl (250 mL x 2) and brine (200 mL x 1), dried and the solvent removed under reduced 40 pressure. The material was carried forward with no further purification to yield ethyl 5- fluoro-2-(2-nitrophenylthio)benzoate 1 (II) (39.3 g, 95 %) as a dark yellow solid. m/z (ES+) M + 1 = 322.1; HPLC t R = 0.88 min. H NMR (300 MHz, DMSO- d6): δ 1.09 (t, 3H, J = 7.2 Hz), 4.16 (q, 2H, J = 7.2 Hz), 7.07 (d, 1H, J = 7.2 Hz), 7.40-7.75 (m, 5H), 8.20 (d, 1H, J = 7.2 Hz).

Step 2: 45 [0056] To a solution of ethyl 5-fluoro-2-(2-nitrophenylthio)benzoate (II) (39.0 g, 121.4 mmol) in MeOH (530 mL) was added tin(II) chloride dihydrate (301 g, 1335.1 mmol) at ambient temperature. The milky yellow suspension was heated

to reflux (65 °C) for 5 hours and then cooled. To the cooled mixture was added EtOAc (1000 mL) and solid Na2CO3 (141.5 g, 1335 mmol). While stirring vigorously, water was slowly added until foaming and tin salt formation ceased. 50 Diatomaceous earth (500 g), was added and the reaction mixture stirred for 30 minutes and filtered. The aqueous layer was removed and the organic layer was washed with brine (500 mL X 1), dried, and the solvent removed under reduced pressure. The material was carried forward with no further purification to yield the aniline ester (III) (17.7 g, 60.6%) as 1 a pale yellow thick syrup. m/z (ES+) M + 1 = 292.1; HPLC t R = 0.88 min. H NMR (300 MHz, DMSO-d6): δ 1.34 (t, 3H, J = 7.2 Hz), 4.35 (q, 2H, J = 7.2 Hz), 5.36 (s, 2H), 6.57-6.70 (m, 2H), 6.85 (d, 1H, J = 9.3 Hz), 7.18-7.35 (m, 3H), 7.70 55 (dd, 1H, 9.3 Hz).

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Step 3:

[0057] To a solution of aniline ester (III) (17.6 g, 60.5 mmol) in toluene (300 mL) was added p-toluenesulfonic acid (11.6 g, 60.5 mmol) at ambient temperature under nitrogen. After heating the mixture at 110 °C for 16 hours, the reaction 5 mixture was cooled to room temperature. The resulting cyclic lactam white precipitate was collected (3.9 g) and the filtrate concentrated under reduced pressure. The reaction mixture was triturated with MeOH (100 mL), the resulting cyclic lactam collected (5.8 g) and the filtrate again concentrated under reduced pressure. The material was purified by

column chromatography over silica gel using 0-10 % of MeOH in CH 2Cl2 as an eluent to give cyclic lactam (1.8 g) as a white powder. The combined product batches gave the final cyclic lactam (IV) (11.2 g, 75%) as an off-white powder. 10 1 m/z (ES+) M + 1 = 246.1; HPLC t R = 0.71 min. H NMR (300 MHz, DMSO-d6): δ 7.15 (t, 1H, J = 9.3 Hz), 7.24 (d, 1H, J = 9.3 Hz), 7.36 (m, 2 H), 7.45 (dd, 1H, J = 9.3 Hz), 7.55 (m, 2H), 10.78 (s, 1H).

Step 4:

15 [0058] A suspension of cyclic lactam (IV) (3.0 g, 12.2 mmol) and N, N-dimethylaniline (0.03 mL, 0.24 mmol) in POCl 3 (6.8 mL, 73.4 mmol) was heated at 125 °C for 2 hours. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in CH2Cl2 (100 mL), washed with cold water (50 mL x 2) and brine (50 mL x 1), dried and the solvent removed under reduced pressure. The material was carried forward with no further purification to yield imino

chloride (V) (3.1 g, 95%) as an amber syrup. m/z (ES+) M + 1 = 264.1; HPLC t R = 0.95 min. 20 Step 5:

[0059] To a solution of imino-chloride (V) (3.0 g, 11.5 mmol) in xylene (115 mL) was added piperazine (7.9 g, 92.2 mmol) at ambient temperature under nitrogen. After heating the mixture at 138 °C for 2 hours, the reaction was cooled 25 to room temperature. The reaction was quenched with 2N HCl to pH 2.0, the acidic aqueous layer separated and washed with CH2Cl2 (2 X 100 mL). To the remaining acidic aqueous layer was added solid K 2CO3 until pH 10 and EtOAc (200 mL) was added. The resulting emulsion was filtered through diatomaceous earth and the organic layer was separated. The organic layer was washed with brine (1 X 50 mL), dried over MgSO4, filtered and concentrated under reduced pressure. The material was purified by column chromatography over silica gel using 0-5 % of 7N NH 3/MeOH in CH2Cl2 30 as an eluent to give the title compound (2.0 g, 55%) as a pale yellow powder. m/z (ES+) M + 1 = 314.2; HPLC t R = 0.50 1 min. H NMR (300 MHz, DMSO-d6): δ 2.68 (m, 2H), 2.72 (m, 2H), 3.40 (m, 4 H), 6.86 (t, 1H, J = 8.1 Hz), 6.98 (d, 1H, J = 8.1 Hz), 7.15-7.38 (br m, 4H), 7.58 (dd, 1H, J = 8.1 Hz), 8.92 (br s, 1H).

Example 1B: 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine 35 [0060] Scheme B shows another contemplated method of preparing 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thi- azepine.

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[0061] Thus, in a three-step process: 2,2’-disulfanediyldianiline may be reacted with 2-bromo-5-fluoro-benzoic acid,

9 EP 2 307 389 B1

as shown, to form 2-(2- amino-phenylsulfanyl)-5-fluorobenzoic acid; the benzoic acid may be cyclized, as shown, to form 2-fluoro-10H-dibenzo[b,f][1,4]thiazepin-11-one, that may be converted to 11- chloro-2-fluoro-dibenzo[b,f][1,4]thiazepine, and that may be reacted with piperazine to form the title compound.

5 Synthesis of 2-fluorodibenzo[b,f][1,4]thiazepin-11(10H)-one:

[0062]

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[0063] A suspension of nickel bromide (5 mmol, 1.09g), bipyridyl (5 mmol, 0.78g) and zinc dust (200 mmol, 13.08g) 20 in 200 mL dry acetonitrile was magnetically stirred, treated with 2,2’- disulfanediyldianiline (52 mmol, 12.92 g) and heated in an oil bath set at 75 °C for 30 min after reaching maximum internal temperature. At the end of this period the reaction mixture was treated with 2-bromo-5-fluorobenzoic acid (100 mmol, 21.90g) in portions and stirred at 75 °C for 1h and the oil bath was removed. The reaction mixture was cooled to the room temperature, transferred to a 1-neck flask and concentrated under reduced pressure. The resulting dark solid was suspended in 200 mL of methanol, cooled in ice 25 and was treated with 100 mL of trifluoro acetic acid by adding through a dropping funnel. The resulting dark solution was stirred for 30 min until all the gas evolution stopped and filtered through a 3 cm pad of diatomous earth and the filtration pad was washed using a total of 400 mL methanol. The resulting gray suspension was refluxed for 1.5 h, cooled to the room temperature and stirred for 16 h and filtered to get a solid. The filtrate was evaporated and the resulting foam was treated with 300 mL 15% ammonium chloride solution and 500 mL ethyl acetate. The suspension was treated 30 with solid sodium bicarbonate until pH 6- 7 (16.8 g, 20 mmol). The resulting white suspension was stirred for 1 h and filtered. The solid residue was washed with 500 mL ethyl acetate. The organic layer from the filtrate was separated from the aqueous layer, dried over sodium sulfate and evaporated to yield an oil which was suspended in 200 mL ether, stirred for 1h, filtered and the solid was washed with 100 mL ether. Combined solids were stirred with 250 mL hot methanol for 10 min and filtered to get the desired 2-(2- aminophenylthio)-5-fluorobenzoic acid as a solid. (17.42g, 66%) 35 MS (M+1) 264; 1H NMR (300 MHz, DMSO- d6) δ ppm 3.06 - 3.45 (m, 1 H) 4.84 - 5.70 (m, 2 H) 6.53 - 6.70 (m, 2 H) 6.82 (d, J=7.6 Hz, 1 H) 7.09 - 7.25 (m, 2 H) 7.26 - 7.36 (m, 1H) 7.66 (dd, J=9.5, 3.0 Hz, 1H). [0064] Asuspension of2-(2- aminophenylthio)-5-fluorobenzoicacid (64.57 mmol, 17.51g)in 500 mL xylenes was treated with p-toluenesulfonic acid monohydrate (64.57 mmol, 12.2g) and heated. Additional 200 mL xylene was added and heating was continued with azeotropic removal of water for 16 h. The resulting pink reaction mixture was cooled to the 40 room temperature and added to 300 mL water. After stirring for 15 min the solid removed by filtration, washed with 3X100 mL water and dried in air for 4 h, and then under high vacuum for 20 h to obtain 2-fluorodibenzo[b,f][1,4]thiazepin-11 1 (10H)-one as a off white solid (11.6 g, 73%); MS (M+1) 246; H NMR (300 MHz, DMSO-d6) δ ppm 7.16 (t, 1 H) 7.24 (d, J=7.2 Hz, 1 H) 7.29 - 7.42 (m, 2 H) 7.47 (dd, J=9.3, 2.9 Hz, 1 H) 7.52 - 7.71 (m, 2 H) 10.47 - 11.20 (m, 1 H), MS 081119 M+1 246(0.72). 45 [0065] 2-Fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine may be prepared from fluorodibenzo 2- [b,f][1,4]thi- azepin-11(10H)-one as described in Steps 4 and 5 of the process of Example la.

Example 1C: 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine

50 [0066] Scheme C shows another contemplated method of preparing 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thi- azepine.

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[0067] Thus, 1-chloro-2-nitrobenzene may be reacted with 4-fluorobezenethiol in the presence of a base to form 1- 25 nitro-2-phenylsulfanyl-(4-fluorobenzene). The nitrofluorobenzene may be reduced to 1- amino-2-phenylsulfanyl-(4-fluor- obenzene) which can converted (for example, as shown) to [2-(4-fluoro-phenylsulfanyl)-phenyl]-carbamic acid phenyl ester. Such an ester may be cyclized as shown to form fluoro-2- 10H-dibenzo[b,f][1,4]thiazepin-11-one, that may be converted to 11-chloro-2-fluoro-dibenzo[b,f][1,4]thiazepine, which can then be reacted with piperazine to form the title compound. 30 Example 1D: 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine

[0068] Scheme D shows yet another contemplated method of preparing 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4] thiazepine. 35

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[0069] Thus, 2-aminobenzenethiol may be reacted with 2,5- difluoro-benzoic acid methyl ester to form a 2-[(E)- 2-amino- 1-eth-(E)-ylidene-but-2-enylsulfanyl]-5-fluoro-benzoic acid methyl ester intermediate that can be converted by treatment with alkali to 2-[(E)- 2-amino-1-eth-(E)-ylidene-but-2-enylsulfanyl]-5-fluoro-benzoic acid. The benzoic acid can be cyclized 30 to form 2-fluoro-10H-dibenzo[b,f][1,4]thiazepin-11-one, that may be converted to 11-chloro-2-fluoro-dibenzo[b,f][1,4] thiazepine, that may be further reacted with piperazine to form the title compound.

Example 2: D2 Assays

35 [0070] In vitro experimental assays can generally be carried out as described herein. Briefly, CHO-K1 cells stably transfected with the dopamine D2s receptor can be used in the experiments and maintained in Ham’s F12 culture medium supplemented with 2 mM L-glutamine, 10% FBS, and 500 mg/ml Hygromycin.

D2 Receptor Binding Assay: 40 [0071] The ability of test compounds to displace 3H-raclopride at the D2s receptor can be determined on membranes from D2s-transfected CHO cells (B max 13 pmol/mg protein). An assay can use a standard 96- well glass-fiber filter plate to retain radioligand bound by the receptor. Retained3H can be determined in a TopCount scintillation plate counter following the addition of a liquid scintillant to each well. Compounds can be evaluated for their potency using competition 45 curve analysis, resulting in calculated Ki values. When this method was carried out generally as described herein with 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine,one average result obtained showedD2 bindingK iat about11 nM.

D2 Receptor in vitro Functional Assays:

50 [0072] GTPgS assay can be performed substantially as described by Lazareno, Methods in Molecular Biology, 1999, 106, 231-245. Antagonist activity of compounds can be determined by the ability of test compounds to block dopamine- stimulated [ 35S]-GTPγS binding to cell membranes from D2s stably- transfected CHO cells. When this method was carried out generally as described therein, with 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine, one average result ob-

tained showed a GTPγS IC50 at about 404 nM. 55 Example 3: D-Amphetamine-induced Hyperlocomotor Activity (LMA)

[0073] Studies in vivo can be used to determine the antipsychotic effect and are generally carried out as follows.

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Briefly, D-Amphetamine-induced Hyperlocomotor Activity (LMA) in a Habituated Rat Model can be assessed in male Long Evans rats using a paradigm that includes a habituation phase followed by administration of 1 mg/kg D-amphet- amine. Animals can be allowed to acclimatize to the testing room for 1 hour before being weighed and placed into activity chambers. Thirty minutes after LMA measurement is begun, animals can be briefly removed, dosed via the sub- cutaneous 5 route (s.c.) with vehicle or test drug at different doses and returned to the chambers. After a further 30 minutes, animals can again be removed and dosed with vehicle or D-amphetamine at 1 mg/kg (s.c.). After returning the animals to the

activity chambers, LMA can be assessed for a further 60 minutes. Haloperidol (0.1 mg/kg dissolved in 2HO) can be administered 15 minutes prior to D-amphetamine via the s.c. route. Statistical analysis can be made of total distance traveled after D- amphetamine administration using ANOVA and Tukey’s post hoc analysis where appropriate. All values 10 can be expressed as Mean and SD. When this method was carried out generally as described therein, 2- fluoro-11-(pip- erazin-1-yl)dibenzo[b,f][1,4]thiazepine showed activity at 30 mg/kg and at 60 mg/kg (sc).

Example 4: Conditioned Avoidance Responding (CAR) Assay

15 [0074] Male Long-Evans rats can be trained to traverse to the opposite side of a standard shuttle cage following presentation of an auditory and visual stimulus to avoid delivery of electric shock to the floor of the cage. Daily sessions can consist of up to 80 trials. If a shock is delivered, animals have the opportunity to escape the shock by traversing to the opposite side of the cage. Drug can be administered (via s.c. or p.o. route) 60 minutes prior to testing and the percentage of trials in which shock is avoided and escaped can be recorded. When this method was carried out generally 20 as described therein, 2- fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine showed activity at 30 mg/kg and at 60 mg/kg (sc).

Example 5: Norepinephrine Uptake

25 [0075] An assay for measuring norepinephrine uptake can be carried out as described below. Briefly, test compounds can be evaluated in an 11-point IC50 curve for their ability to inhibit uptake of a proprietary fluorescent substrate (dye) from Molecular Devices that mimics biogenic amine neurotransmitters. A stable population of HEK293F cells transfected with the human norepinephrine transporter (cultured in Freestyle 293 expression medium with 75 mg/ml hygromycin B) can be cryopreserved, then plated and used on the day of the assay. Cells can be at 60K/ well; dye can be 7% (final) of 30 the vendor-recommended reconstitution volume (100%). Compounds can be diluted 1:20 in buffer and incubated with the cells for 30 minutes prior to addition of the dye. In this fluorescence intensity assay, plates can be read after a 20 minute dye incubation to determine percent effect with respect to total signal (0.5% DMSO, final) and background signal (10 mM , final). The IC50, half of the control response, can be converted to Ki using the using the standard Cheng-Prusoff equation. When this method was carried out generally as described herein with 2-fluoro-11-(piperazin- 35 1-yl)dibenzo[b,f][1,4]thiazepine, one average result obtained showed NET inhibition K i at about 10 nM.

Example 6: H1 Receptor Binding

[0076] The H1 receptor binding method can be carried out in accordance with De Backer et al., Biochem. Biophys. 40 Res. Commun., 1993, 197(3), 1601. When this method was carried out generally as described therein with 2-fluoro- 11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine, one average result obtained showed H1 binding K i at about 7.1 nM. [0077] Pharmacological data concerning the compound of Example 1 and other piperazin-1-yl-dibenzo[b,f][1,4]thi- azepines (reference examples) is shown in the table below.

45 Compound hNET uptake D2 Ant GTPγS D2 Ant GTPγS D2 binding w/ H1Hu Bind Mean Name HEK FLInt CR IC50 Top Effect NaCl Ki (nM) Ki (M) Mean Ki (M) (Example 1) 2- 9.60E-09 4.00E-07 97 11 7.12E-09

50 Fluoro-11- piperazin-1-yl- dibenzo[b,f] [1,4]thiazepine 11-Piperazin- 2.40E-08 8.00E-07 89 37 3.33E-09 55 1-yl-dibenzo[b, f][1,4] thiazepine

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(continued)

Compound hNET uptake D2 Ant GTPγS D2 Ant GTPγS D2 binding w/ H1Hu Bind Mean Name HEK FLInt CR IC50 Top Effect NaCl Ki (nM) Ki (M) 5 Mean Ki (M) 2-Chloro-11- 4.10E-08 3.50E-08 100 0.7 3.87E-09 piperazin-1-yl- dibenzo[b,f] [1,4]thiazepine 10 2-Chloro- 5.10E-08 8.40E-09 100 0.49 1.52E-09 11-(4-methyl- piperazin-1- yl)-dibenzo[b,f] 15 [1,4]thiazepine 2-Fluoro- 5.90E-08 1.70E-07 100 1.1 1.82E-09 11-(4-methyl- piperazin-1- yl)-dibenzo[b,f] 20 [1,4]thiazepine

Claims

25 1. A compound of Formula I:

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wherein Z is H, that is 2-fluoro-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepine, or a pharmaceutically acceptable salt thereof.

45 2. A pharmaceutical composition comprising a compound, or a pharmaceutically acceptable salt thereof, according to claim 1, and at least one pharmaceutically acceptable carrier.

3. Use of a compound according to claim 1, or a pharmaceutically acceptable salt thereof, for the manufacture of a medicament, for treating a psychiatric disorder. 50 4. The use according to claim 3 wherein the psychiatric disorder is bipolar disorder, an anxiety disorder, a mood disorder or schizophrenia or other psychotic disorder.

5. The use according to claim 4 wherein the psychiatric disorder is bipolar disorder. 55 6. The use according to claim 4 wherein the psychiatric disorder is schizophrenia.

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Patentansprüche

1. Verbindung der Formel I:

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wobei Z für H steht, das heißt 2-Fluor-11-(piperazin-1-yl)dibenzo[b,f][1,4]thiazepin, oder ein pharmazeutisch an- nehmbares Salz davon.

25 2. Pharmazeutische Zusammensetzung, umfassend eine Verbindung oder ein pharmazeutisch annehmbares Salz davon nach Anspruch 1 und mindestens einen pharmazeutisch annehmbaren Träger.

3. Verwendung einer Verbindung nach Anspruch 1 oder eines pharmazeutisch annehmbaren Salzes davon zur Her- stellung eines Medikaments zur Behandlung einer psychiatrischen Erkrankung. 30 4. Verwendung nach Anspruch 3, wobei es sich bei der psychiatrischen Erkrankung um eine bipolare Störung, eine Angststörung, eine Stimmungsstörung oder Schizophrenie oder eine andere psychotische Störung handelt.

5. Verwendung nach Anspruch 4, wobei es sich bei der psychiatrischen Störung um eine bipolare Störung handelt. 35 6. Verwendung nach Anspruch 4, wobei es sich bei der psychiatrischen Störung um Schizophrenie handelt.

Revendications 40 1. Composé de Formule I :

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55 dans laquelle Z est H, c’est-à-dire la 2-fluoro-11-(pipérazin-1-yl)dibenzo[b,f][1,4]thiazépine, ou un sel pharmaceu- tiquement acceptable de celui-ci.

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2. Composition pharmaceutique comprenant un composé, ou un sel pharmaceutiquement acceptable de celui- ci, selon la revendication 1, et au moins un support pharmaceutiquement acceptable.

3. Utilisation d’un composé selon la revendication 1, ou d’un sel pharmaceutiquement acceptable de celui- ci, pour la 5 fabrication d’un médicament destiné au traitement d’un trouble psychiatrique.

4. Utilisation selon la revendication 3, caractérisée en ce que le trouble psychiatrique est le trouble bipolaire, un trouble de l’anxiété, un trouble de l’humeur ou la schizophrénie ou autre trouble psychotique.

10 5. Utilisation selon la revendication 4, caractérisée en ce que le trouble psychiatrique est le trouble bipolaire.

6. Utilisation selon la revendication 4, caractérisée en ce que le trouble psychiatrique est la schizophrénie.

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REFERENCES CITED IN THE DESCRIPTION

This list of references cited by the applicant is for the reader’s convenience only. It does not form part of the European patent document. Even though great care has been taken in compiling the references, errors or omissions cannot be excluded and the EPO disclaims all liability in this regard.

Patent documents cited in the description

• CH 422793 [0003] • EP 447857 A [0006] • NL 293210 [0003] • US 20060063754 A [0006] • US 7491715 B1 [0003] • FR 1508536 [0006] • US 7517871 B1 [0003] • US 3539573 A [0006]

Non-patent literature cited in the description

• Behavioral Approach to Nondyskinetic Antagonists: • American Psychiatric Association: Diagnostic and Identification of Seroquel. J. Med. Chem., 2001, vol. Statistical Manual of Mental Disorders. American 44, 372-380 [0003] Psychiatric Association, 2000 [0040] • Remington’s Pharmaceutical Sciences. Mack Pub- • LAZARENO. Methods in Molecular Biology, 1999, lishing Company, 1975 [0036] vol. 106, 231-245 [0072] • DE BACKER et al. Biochem. Biophys. Res. Com- mun., 1993, vol. 197 (3), 1601 [0076]

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