LEGENDScreen™ Human and Mouse PE Kits

Introducing LEGENDScreen™ Human and Mouse PE Kits LEGENDScreen™ is the most cost-effective way to test this many directly-conjugated antibodies in a single assay. These kits can be used to screen cell lines and primary cells (PBMCs, bone marrow-derived cells, and cells isolated from tissues such as spleen and lymph node). The workflow is simple and easy. Just reconstitute the antibodies, add your cells, collect the samples, and then analyze. Cell staining is done directly in the plates, so no additional transfer of reconstituted antibody is required. The LEGENDScreen™ Human and Mouse Cell PE Kits are convenient, easy-to-use, and powerful tools for immunology, BioLegend’s LEGENDScreen™ products are lyophilized, fluorophore-conjugated antibodies stem cell, cancer research, and several other fields of research. provided in 96-well plates for the purpose of screening cell surface molecules on your cells of interest. Customize LEGENDScreen™ Individual fluorophore-conjugated antibodies or antibody We understand your needs for customization of research tools cocktails have been used as powerful tools for immunology, to fit your specific requirements. Our Custom Solutions Team cancer, and stem cell research. However, there is an increasing can customize LEGENDScreen™ products to include only the need for tools that can quickly screen cells for a large number of specificities that you want and provide them in bulk sizing. cell surface markers. The BioLegend LEGENDScreen™ Human and Mouse PE Kits are made to meet this need. Product Size Cat. No. LEGENDScreen™ Human PE Kit 1 kit (1 test per antibody) 700007 Why choose LEGENDScreen™? LEGENDScreen™ Mouse PE Kit 1 kit (1 test per antibody) 700005 • Large selection of specificities per kit. The Human PE Kit Learn more about LEGENDScreen™ at: biolegend.com/legendscreen has 361 cell surface markers plus 10 isotype controls. The Mouse PE Kit has 255 cell surface marker antibodies plus • Plate Maps 11 isotype controls. • Antibody Specificities and Clones • Fast and easy-to-follow protocol. • Protocols • Directly conjugated antibodies at pre-titrated optimal • FAQs concentrations provide reliable results. • Performance Data • Complete kits with staining buffer, fixation buffer, and plate sealers.

biolegend.com 07-0054-04 LEGENDScreen™ Human and Mouse PE Kits

Specificity Hu Mo Specificity Hu Mo Specificity Hu Mo Specificity Hu Mo Specificity Hu Mo Specificity Hu Mo Specificity Hu Mo Specificity Hu Mo CD172a/b Delta Opioid 33D1 • CD45RO • CD104 • • • CD268 • • • Ly-6G/Ly-6C • TMEM8A • 4-1BB Ligand • • CD45R/B220 • CD105 • • (SIRPα/β) CD269 • Receptor Ly-6K • TNAP • CD172g DLL1 • • APCDD1 • CD45.1 • CD106 • • • CD271 • Ly-49A • TRA-1-60-R • (SIRPγ) DLL4 • • B7-H4 • • CD45.2 • CD107a • • CD272 • • Ly-49C/F/I/H • TRA-1-81 CD178 • • Dopamine • C3aR • CD46 • CD107b • CD273 • Ly49D • CD179a • • Receptor D1 • TRA-2-49 • Cadherin 11 • CD47 • • CD109 • CD274 • • Ly49H • CD179b • (DRD1) TRA-2-54 • CCRL2 • CD48 • • CD111 • CD275 • • Ly-51 CD180 DR3 • • • Trem-like 4 CCX-CKR • • • CD49a • • CD112 • CD276 • • E-Cadherin • Ly108 • (CCRL1) • CD181 • TSLPR • CD49b • • CD114 • CD277 • EGFR • Lymphotoxin CD1a • CD182 • • β Receptor • VEGFR-3 • CD49c • CD115 • • CD278 • • EphA2 • CD1b • CD183 • • Mac-2 • VISTA • CD49d • • CD116 • CD279 • • erbB3 • CD1c • CD184 • • Mac-3 • XCR1 • • CD49e • • CD117 • • CD282 • ESAM • CD1d • • CD185 • • MAdCAM-1 • β2- CD49f • CD119 • CD284 • F4/80 • • CD2 • • CD186 • • MAIR-IV microglobulin CD50 • CD120a • CD290 • FcεRIα • • • Isotype Controls CD3 • • CD51 CD191 • CD294 MAIR-V • • CD120b • • • FPR3 • CD3ε CD192 • Armenian • CD52 • CD121a • CD298 • MD-1 • • FR4 • Hamster IgG • • CD4 • • CD54 CD193 • • CD300c MERTK • • • • CD122 • • • Galectin-9 • Isotype Ctrl CD5 • • CD194 • • MICA/MICB CD55 • • CD123 • • CD300c/d • Ganglioside • Mouse IgG1, κ CD6 • • CD195 • • • CD56 (NCAM) • CD124 • • • • CD300LG • GD2 MRGX2 • Isotype Ctrl CD7 • CD57 CD196 • • GARP MSC • Mouse IgG2a, • CD126 • • CD301 • • • • • • CD8a • • κ Isotype Ctrl CD58 • CD127 • • CD197 • • CD301b • GITR Ligand • MSC,NPC • CD8b • Mouse IgG2b, CD59 • CD129 • CD198 • • CD303 • GL7 • MUC-13 • • • CD9 κ Isotype Ctrl • • CD59a • CD130 CD199 • • GPR19 NKG2D • • • CD304 • • • Mouse IgG3, κ CD10 • CD61 CD200 NKp80 • • CD131 • • • CD307e • GPR56 • • Isotype Ctrl • CD11a • • NK-1.1 CD62E • CD132 • • CD200R • • CD309 • • GPR83 • • Mouse IgM, κ CD11b • • Notch 1 • • CD62L • • CD133 • CD200R3 • CD314 • H-2 • • • Isotype Ctrl CD11c • • CD62P • CD134 • • CD201 • CD317 • • Notch 2 • • Rat IgG1, κ CD13 • HLA-A,B,C • • • CD63 • • CD135 • • CD202b • • CD318 • Notch 3 • • Isotype Ctrl CD14 • • HLA-DR • Rat IgG2a, κ CD64 • • CD137 • • CD203c • CD319 • • Notch 4 • • • • CD15 • HLA-E • Isotype Ctrl CD66a • CD138 • • CD205 • • CD323 • NPC • CD16 HVEM Rat IgG2b, κ • CD66a/c/e • CD206 • • PDC-TREM • CD140a • • • CD324 • Isotype Ctrl • • CD16/32 • CD66b I-A/I-E • PD-1H • • CD140b • • CD207 • • CD325 • Rat IgG2c, κ CD18 • • PIR-A/B • CD69 • • CD141 • CD209 • CD326 • • IFNAR-1 • • Isotype Ctrl CD19 • • Plexin B2 CD70 • • CD142 • CD210 • • CD328 • IFN-γ R β • Rat IgM, κ CD20 • • • • Podoplanin • • CD71 • • CD143 • CD213α1 • CD334 • chain • • Isotype Ctrl CD21 • Ig light PSMA Syrian CD73 • • CD144 • • CD213α2 • CD335 • • • • • CD21, CD35 • chain κ RAE-1γ Hamster IgG • CD74 • CD146 CD336 • • • CD215 • • Isotype Ctrl CD22 • • CD79b • • Ig light ROR1 • CD147 • CD217 • CD337 • chain λ • CD23 • • CD80 Sialyl Lewis X • • CD148 • CD218a CD338 • • CD24 • • • IgD • • (dimeric) CD81 • • CD150 • • CD220 • CD339 • IgG Fc Siglec H CD25 • • CD82 • • • CD151 CD340 • CD221 • • IgM Siglec-8 CD26 • CD83 • • • • • CD152 • • CD223 • • CD344 • Siglec-9 CD27 • • CD84 • • IL-21R • • CD153 • CD226 • • CD351 • Siglec-10 CD28 • • CD85 • IL-23R • • CD154 CD227 CD352 • CD29 • • • SSEA-1 • • • CD85d • IL-28RA • CD155 CD229 CD354 • SSEA-3 CD30 • • CD85h • • • • • • IL-33Rα CD156c • CD355 • SSEA-4 CD31 • • CD85k • • CD230 (Prion) • • • • CD157 Integrin α9β1 • SSEA-5 CD32 • CD86 • • • CD231 • CD357 • • • Integrin β5 CD33 CD158 • • SUSD2 • • CD87 • CD235ab • CD360 • CD34 CD158b Integrin β7 • • TACSTD2 • • • CD88 • • • CD243 • CD365 • • TCR Vγ1.1 + CD35 • CD89 • CD158e1 Jagged 2 • • • CD244 (2B4) • CD366 • • Vγ1.2 • CD36 • • CD90 • CD159a JAML • • CD245 • CD368 TCR α/β • CD36L1 • CD90.1 • • CD160 • • CD252 • • KLRG1 • • CD37 • CD90.2 CD369 • • TCR β chain • • Ksp37 CD38 CD161 • CD253 • • • • CD92 • CD370 • • TCR γ/δ • • CD162 • LAP • • CD39 • • CD93 • • CD254 • • TER-119 • CD371 • • LOX-1 • CD40 • • CD94 • • CD163 • CD255 • • TIGIT • CLEC4A • LPAM-1 • CD41 • • CD95 • CD164 • CD258 • TIGIT (VSTM3) • CX3CR1 • • LT β R CD42b • CD96 • • CD165 • CD261 • • Tim-2 • CD43 CXCL16 • LY6G6D • • • CD97 • CD166 • CD262 • • Tim-4 • • CD44 CXCR7 • Ly-6A/E • • • CD98 • CD169 • • CD263 • TLR4 (CD284)/ DcTRAIL-R1 • Ly-6C • MD2 Complex • CD45 • • CD99 • CD170 • CD265 • DcTRAIL-R2 • Ly-6D TLT-2 CD45RA • CD100 • • CD172a CD266 • • • • • CD45RB • • CD102 • (SIRPα) CD267 • • Ly-6G • TM4SF20 • This list may be subject to change. For updated specificity list, visit:biolegend.com/legendscreen