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Evaluation of the Antioxidant Activity of Extracts and Flavonoids Obtained from Bunium Alpinum Waldst
DOI: 10.1515/cipms-2017-0001 Curr. Issues Pharm. Med. Sci., Vol. 30, No. 1, Pages 5-8 Current Issues in Pharmacy and Medical Sciences Formerly ANNALES UNIVERSITATIS MARIAE CURIE-SKLODOWSKA, SECTIO DDD, PHARMACIA journal homepage: http://www.curipms.umlub.pl/ Evaluation of the antioxidant activity of extracts and flavonoids obtained from Bunium alpinum Waldst. & Kit. (Apiaceae) and Tamarix gallica L. (Tamaricaceae) Mostefa Lefahal1, Nabila Zaabat1, Lakhdar Djarri1, Merzoug Benahmed2, Kamel Medjroubi1, Hocine Laouer3, Salah Akkal1* 1 Université de Constantine 1, Unité de Recherche Valorisation des Ressources Naturelles Molécules Bioactives et Analyses Physico- Chimiques et Biologiques, Département de Chimie, Facultés des Sciences Exactes, Algérie 2 Université Larbi Tébessi Tébessa Laboratoire des Molécules et Applications, Algérie 3 Université Ferhat Abbas Sétif 1, Laboratoire de Valorisation des Ressources Naturelles Biologiques. Le Département de Biologie et d'écologie Végétales, Algérie ARTICLE INFO ABSTRACT Received 19 October 2016 The aim of the present work was to evaluate the antioxidant activity of extracts and four Accepted 24 January 2017 flavonoids that had been isolated from the aerial parts of Bunium alpinum Waldst. et Kit. Keywords: (Apiaceae) and Tamarix gallica L. (Tamaricaceae). In this work, the four flavonoids were B. alpinum, first extracted via various solvents, then purified through column chromatography (CC) T. gallica, and thin layer chromatography (TLC). The four compounds were subsequently identified flavonoids, 1 13 antioxidant activity, by spectroscopic methods, including: UV, mass spectrum H NMR and C NMR. DPPH assay, The EtOAc extract ofBunium alpinum Waldst. et Kit yielded quercetin-3-O-β-glucoside EC50. (3',4',5,7-Tetrahydroxyflavone-3-β-D-glucopyranoside) (1), while the EtOAc and n-BuOH extracts of Tamarix gallica L. -
Rhamnazin Inhibits Proliferation and Induces Apoptosis of Human Jurkat Leukemia Cells in Vitro
експериментальні роботи UDC 577.152.3 + 576.38 doi: http://dx.doi.org/10.15407/ubj87.06.122 RHAMNAZIN INHIBITS PROLIFERATION AND INDUCES APOPTOSIS OF HUMAN JURKAT LEUKEMIA CELLS IN VITRO А. А. Philchenkov, М. P. Zavelevych R. e. kavetsky institute of experimental Pathology, oncology and Radiobiology, national academy of Sciences of Ukraine, kyiv; e-mail: [email protected] a ntiproliferative and apoptogenic effects of rhamnazin, a dimethoxylated derivative of quercetin, were studied in human acute lymphoblastic leukemia Jurkat cells. The cytotoxicity and apoptogenic activity of rhamnazin in vitro are inferior to that of quercetin. The apoptogenic activity of rhamnazin is realized via mitochondrial pathway and associated with activation of caspase-9 and -3. The additive apoptogenic effect of rhamnazin and suboptimal doses of etoposide, a Dna topoisomerase ii inhibitor, is demonstrated. Therefore, methylation of quercetin modifies its biological effects considerably. k e y w o r d s: acute lymphoblastic leukemia, flavonoids, cell cycle, apoptosis, caspases, flow cytometry. lavonoids constitute the largest class (over mon natural substances. Monomethylated deriva 6,500 compounds) of biologically active plant tives have been shown to inhibit proliferation and F polyphenols [1]. Most of them by far are de induce apoptosis in cancer cells [8], or to sensitize rivatives of 2-phenylbenzopyran (flavan) or 2-phe malignant cells to other cytotoxic agents [9]. Yet nylbenzopyran-4-one (flavone). Flavonoids display there is only a limited amount of data concerning antioxidant properties and may also induce apoptotic efficiency of rhamnazin (3′,7-dimethylquercetin), a cell death, depending on concentration [2]. It has quercetin derivative differing only by two methyl been demonstrated that the proapoptotic and cyto groups (Fig. -
Bioactive Components and Health Effects of Pecan Nuts and Their By- Products: a Review
Journal of International Society for Food Bioactives Nutraceuticals and Functional Foods Review J. Food Bioact. 2018;1:56–92 Bioactive components and health effects of pecan nuts and their by- products: a review Emilio Alvarez-Parrillaa, Rafael Urrea-Lópezb and Laura A. de la Rosaa* aDepartment of Chemical Biological Sciences, Universidad Autónoma de Ciudad Juárez, AnilloEnvolvente del Pronaf y Estocolmo, s/n, Cd, 32310 Juárez, Chihuahua, Mexico bCIATEJ, UnidadNoreste, Autopista Monterrey-Aeropuerto km 10.Parque PIIT. Vía de Innovación 404. Apodaca, N.L. México *Corresponding author: Laura A. de la Rosa, Department of Chemical Biological Sciences, Universidad Autónoma de Ciudad Juárez, AnilloEnvolvente del Pronaf y Estocolmo, s/n, Cd, 32310 Juárez, Chihuahua, Mexico. Tel: (+52) 656-688-1800 ext 1563; E-mail: ldelaros@ uacj.mx DOI: 10.31665/JFB.2018.1127 Received: January 18, 2018; Revised received & accepted: January 21, 2018 Citation: Alvarez-Parrilla, E., Urrea-López, R., and de la Rosa, L.A. (2018). Bioactive components and health effects of pecan nuts and their by-products: a review. J. Food Bioact. 1: 56–92. Abstract Pecan is a North American native tree that produces a stone fruit or kernel, commonly known as pecan nut,which is highly valuable worldwide due to its sensory quality, and health promoting properties derived from the pres- ence of mono- and polyunsaturated fatty acids, tocopherols and monomeric and polymeric polyphenolic com- pounds. The increase in the demand for pecan nut leads to an increase in by-products such as leaves, cake and principally nutshell, which have high contents of bioactive components, making them interesting raw materials to produce nutraceuticals with health benefits. -
Chondroprotective Agents
Europaisches Patentamt J European Patent Office © Publication number: 0 633 022 A2 Office europeen des brevets EUROPEAN PATENT APPLICATION © Application number: 94109872.5 © Int. CI.6: A61K 31/365, A61 K 31/70 @ Date of filing: 27.06.94 © Priority: 09.07.93 JP 194182/93 Saitama 350-02 (JP) Inventor: Niimura, Koichi @ Date of publication of application: Rune Warabi 1-718, 11.01.95 Bulletin 95/02 1-17-30, Chuo Warabi-shi, 0 Designated Contracting States: Saitama 335 (JP) CH DE FR GB IT LI SE Inventor: Umekawa, Kiyonori 5-4-309, Mihama © Applicant: KUREHA CHEMICAL INDUSTRY CO., Urayasu-shi, LTD. Chiba 279 (JP) 9-11, Horidome-cho, 1-chome Nihonbashi Chuo-ku © Representative: Minderop, Ralph H. Dr. rer.nat. Tokyo 103 (JP) et al Cohausz & Florack @ Inventor: Watanabe, Koju Patentanwalte 2-5-7, Tsurumai Bergiusstrasse 2 b Sakado-shi, D-30655 Hannover (DE) © Chondroprotective agents. © A chondroprotective agent comprising a flavonoid compound of the general formula (I): (I) CM < CM CM wherein R1 to R9 are, independently, a hydrogen atom, hydroxyl group, or methoxyl group and X is a single bond or a double bond, or a stereoisomer thereof, or a naturally occurring glycoside thereof is disclosed. The 00 00 above compound strongly inhibits proteoglycan depletion from the chondrocyte matrix and exhibits a function to (Q protect cartilage, and thus, is extremely effective for the treatment of arthropathy. Rank Xerox (UK) Business Services (3. 10/3.09/3.3.4) EP 0 633 022 A2 BACKGROUND OF THE INVENTION 1 . Field of the Invention 5 The present invention relates to an agent for protecting cartilage, i.e., a chondroprotective agent, more particularly, a chondroprotective agent containing a flavonoid compound or a stereoisomer thereof, or a naturally occurring glycoside thereof. -
Occurrence of Pathogenic and Endophytic Fungi and Their Influence on Quality of Medicinal Plants Applied in Management of Neurological Diseases and Mental Disorders
From Botanical to Medical Research Vol. 63 No. 4 2017 Received: 2017-08-06 DOI: 10.1515/hepo-2017-0025 Accepted: 2017-12-16 Review article Occurrence of pathogenic and endophytic fungi and their influence on quality of medicinal plants applied in management of neurological diseases and mental disorders KATARZYNA WIELGUSZ1, LIDIA IRZYKOWSKA2* 1Institute of Natural Fibers and Medicinal Plants Department of Breeding and Agriculture of Fibrous and Energetic Plants Wojska Polskiego 71b 60-630 Poznań, Poland 2Poznan University of Life Sciences Department of Phytopathology, Seed Science and Technology Dąbrowskiego 159 60-594 Poznań, Poland *corresponding author: phone: 48 61 848 79 27, fax: 48 61 848 79 99, e-mail: [email protected] Summary Due to increasing demand of medicinal plants (MPs), quality and safety more attention to the plant health should be paid. Among herb pathogens, especially fungi cause serious diseases in these plants decreasing yield and quality of herbal raw material. Some species, i.e. Fusarium sp., Alternaria sp., Penicillium sp. are known as mycotoxin producers. Paradoxically, self-treatment with herbal raw material can expose the pa- tient to mycotoxin activity. In tissues of some MPs species, asymptomatically endophytic fungi residue. It is known that they are able to influence a biosynthesis of secondary metabolites in their host plant or produce biologically active compounds. Until recently these microorganisms have been neglected as a component of MPs, the reason why there have unexplored bioactivity and biodiversity. The paper presents an overview of herbal plants that are used in the treatment of nervous system diseases. Pathogenic fungi that infect these plants are described. -
Metabolites OH
H OH metabolites OH Article Genomic Survey, Transcriptome, and Metabolome Analysis of Apocynum venetum and Apocynum hendersonii to Reveal Major Flavonoid Biosynthesis Pathways Gang Gao , Ping Chen, Jikang Chen , Kunmei Chen, Xiaofei Wang, Aminu Shehu Abubakar, Ning Liu, Chunming Yu * and Aiguo Zhu * Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China; [email protected] (G.G.); [email protected] (P.C.); [email protected] (J.C.); [email protected] (K.C.); [email protected] (X.W.); [email protected] (A.S.A.); [email protected] (N.L.) * Correspondence: [email protected] (C.Y.); [email protected] (A.Z.); Tel.: +86-0731-8899-8507 (C.Y. & A.Z.) Received: 8 October 2019; Accepted: 2 December 2019; Published: 5 December 2019 Abstract: Apocynum plants, especially A. venetum and A. hendersonii, are rich in flavonoids. In the present study, a whole genome survey of the two species was initially carried out to optimize the flavonoid biosynthesis-correlated gene mining. Then, the metabolome and transcriptome analyses were combined to elucidate the flavonoid biosynthesis pathways. Both species have small genome sizes of 232.80 Mb (A. venetum) and 233.74 Mb (A. hendersonii) and showed similar metabolite profiles with flavonols being the main differentiated flavonoids between the two specie. Positive correlation of gene expression levels (flavonone-3 hydroxylase, anthocyanidin reductase, and flavonoid 3-O-glucosyltransferase) and total flavonoid content were observed. The contents of quercitrin, hyperoside, and total anthocyanin in A. venetum were found to be much higher than in A. hendersonii, and such was thought to be the reason for the morphological difference in color of A. -
Endophytic Fungi Harbored in Camptotheca Acuminata
Endophytic fungi harbored in Camptotheca acuminata, Hypericum perforatum and Juniperus communis plants as promising sources of camptothecin, hypericin and deoxypodophyllotoxin D I S S E R T A T I O N Submitted for the degree of Dr. rer. nat. (rerum naturalium) to the Faculty of Chemistry Technische Universität Dortmund by Souvik Kusari 2010 Endophytic fungi harbored in Camptotheca acuminata, Hypericum perforatum and Juniperus communis plants as promising sources of camptothecin, hypericin and deoxypodophyllotoxin APPROVED DISSERTATION Doctoral Committee Chairman: Prof. Dr. Carsten Strohmann Reviewers: 1. Prof. Dr. Dr.h.c. Michael Spiteller 2. Prof. Dr. Oliver Kayser Date of defense examination: October 04, 2010 Chairman of the examination: Prof. Dr. Christof M. Niemeyer “The grand aim of all science is to cover the greatest number of empirical facts by logical deduction from the smallest number of hypotheses or axioms” Albert Einstein (March 14, 1879 – April 18, 1955) THIS THESIS IS DEDICATED TO MY PARENTS … i Declaration Declaration I hereby declare that this thesis is a presentation of my original research work, and is provided independently without any undue assistance. Wherever contributions of others are involved, every effort is made to indicate this clearly, with due reference to the literature(s), and acknowledgement of collaborative research and discussions. This work was done under the guidance and supervision of Professor Dr. Dr.h.c. Michael Spiteller, at the Institute of Environmental Research (INFU) of the Faculty of Chemistry, Chair of Environmental Chemistry and Analytical Chemistry, TU Dortmund, Germany. Dated: August 10, 2010 SOUVIK KUSARI Place: Dortmund, Germany In my capacity as supervisor of the candidate’s thesis, I certify that the above statements are true to the best of my knowledge. -
Flavonol Glycosides from Clematis Cultivars and Taxa, and Their Contribution to Yellow and White Flower Colors
Bull. Natl. Mus. Nat. Sci., Ser. B, 34(3), pp. 127–134, September 22, 2008 Flavonol Glycosides from Clematis Cultivars and Taxa, and Their Contribution to Yellow and White Flower Colors Masanori Hashimoto1, Tsukasa Iwashina1,2,*, Junichi Kitajima3 and Sadamu Matsumoto2 1 Graduate School of Agriculture, Ibaraki University, Ami 300–0393, Japan 2 Department of Botany, National Museum of Nature and Science, Amakubo 4–1–1, Tsukuba 305–0005, Japan 3 Laboratory of Pharmacognosy, Showa Pharmaceutical University, Higashi-tamagawagakuen 3, Machida, Tokyo 194–8543, Japan * Corresponding author: E-mail: [email protected] Abstract The flower pigments in two yellow Clematis cultivars, “Gekkyuden” and “Manshu-ki”, and a yellow flower type of C. patens collected in Korea, were characterized. They were compared with those of three white Clematis florida varieties, var. florida, var. florepleno and var. sieboidiana. It was shown by UV-visible spectral survey of crude MeOH extract of their sepals that carotenoid pigment is apparently absent from yellow flowers. High performance liquid chromato- graphical and paper chromatographical survey of the flower pigments showed the presence of the flavonol glycosides. They were isolated and characterized by UV spectroscopy, acid hydrolysis, LC-MS, and direct HPLC and TLC comparisons with authentic samples. Quercetin 3-O-galacto- side (6) and 3-O-glucoside (7) were isolated from two yellow cultivars and a yellow flower type of C. patens as major components together with minor quercetin 3-O-rutinoside (8). On the other hand, kaempferol 3-O-rutinoside (9) and 3-O-glucoside (4) were detected in the white C. florida varieties as major compounds. -
Identification and Bioactive Potential of Endophytic Fungi Isolated From
Qadri et al. SpringerPlus 2013, 2:8 http://www.springerplus.com/content/2/1/8 a SpringerOpen Journal RESEARCH Open Access Identification and bioactive potential of endophytic fungi isolated from selected plants of the Western Himalayas Masroor Qadri1, Sarojini Johri1, Bhahwal A Shah2, Anamika Khajuria3, Tabasum Sidiq3, Surrinder K Lattoo4, Malik Z Abdin5 and Syed Riyaz-Ul-Hassan1* Abstract This study was conducted to characterize and explore the endophytic fungi of selected plants from the Western Himalayas for their bioactive potential. A total of 72 strains of endophytic fungi were isolated and characterized morphologically as well as on the basis of ITS1-5.8S-ITS2 ribosomal gene sequence acquisition and analyses. The fungi represented 27 genera of which two belonged to Basidiomycota, each representing a single isolate, while the rest of the isolates comprised of Ascomycetous fungi. Among the isolated strains, ten isolates could not be assigned to a genus as they displayed a maximum sequence similarity of 95% or less with taxonomically characterized organisms. Among the host plants, the conifers, Cedrus deodara, Pinus roxburgii and Abies pindrow harbored the most diverse fungi, belonging to 13 different genera, which represented almost half of the total genera isolated. Several extracts prepared from the fermented broth of these fungi demonstrated strong bioactivity against E. coli and S. aureus with the lowest IC50 of 18 μg/ml obtained with the extract of Trichophaea abundans inhabiting Pinus sp. In comparison, extracts from only three endophytes were significantly inhibitory to Candida albicans, an important fungal pathogen. Further, 24 endophytes inhibited three or more phytopathogens by at least 50% in co-culture, among a panel of seven test organisms. -
Figure S1. Heat Map of R (Pearson's Correlation Coefficient)
Figure S1. Heat map of r (Pearson’s correlation coefficient) value among different samples including replicates. The color represented the r value. Figure S2. Distributions of accumulation profiles of lipids, nucleotides, and vitamins detected by widely-targeted UPLC-MC during four fruit developmental stages. The colors indicate the proportional content of each identified metabolites as determined by the average peak response area with R scale normalization. PS1, 2, 3, and 4 represents fruit samples collected at 27, 84, 125, 165 Days After Anthesis (DAA), respectively. Three independent replicates were performed for each stages. Figure S3. Differential metabolites of PS2 vs PS1 group in flavonoid biosynthesis pathway. Figure S4. Differential metabolites of PS2 vs PS1 group in phenylpropanoid biosynthesis pathway. Figure S5. Differential metabolites of PS3 vs PS2 group in flavonoid biosynthesis pathway. Figure S6. Differential metabolites of PS3 vs PS2 group in phenylpropanoid biosynthesis pathway. Figure S7. Differential metabolites of PS4 vs PS3 group in biosynthesis of phenylpropanoids pathway. Figure S8. Differential metabolites of PS2 vs PS1 group in flavonoid biosynthesis pathway and phenylpropanoid biosynthesis pathway combined with RNA-seq results. Table S1. A total of 462 detected metabolites in this study and their peak response areas along the developmental stages of apple fruit. mix0 mix0 mix0 Index Compounds Class PS1a PS1b PS1c PS2a PS2b PS2c PS3a PS3b PS3c PS4a PS4b PS4c ID 1 2 3 Alcohols and 5.25E 7.57E 5.27E 4.24E 5.20E -
Fungal Endophytes Œ Secret Producers of Bioactive Plant
REVIEWS Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich-Heine-Universität Düsseldorf, Germany Fungal endophytes – secret producers of bioactive plant metabolites A. H. Aly, A. Debbab, P. Proksch Received November 27, 2012, accepted December 18, 2012 Prof. Dr. Peter Proksch, Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstrasse 1, Geb. 26.23, 40225 Düsseldorf, Germany [email protected] Dedicated to Professor Theo Dingermann, Frankfurt, on the occasion of his 65th birthday. Pharmazie 68: 499–505 (2013) doi: 10.1691/ph.2013.6517 The potential of endophytic fungi as promising sources of bioactive natural products continues to attract broad attention. Endophytic fungi are defined as fungi that live asymptomatically within the tissues of higher plants. This overview will highlight the uniqueness of endophytic fungi as alternative sources of pharmaceutically valuable compounds originally isolated from higher plants, e.g. paclitaxel, camptothecin and podophyllotoxin. In addition, it will shed light on the fungal biosynthesis of plant associated metabolites as well as new approaches developed to improve the production of commercially important plant derived compounds with the involvement of endophytic fungi. 1. Introduction as potential new sources for therapeutic agents (Aly et al. 2010, 2011; Debbab et al. 2011, 2012) and set the stage for a more Fungal endophytes were first defined by Anton de Bary in 1886 comprehensive examination of the ability of other plants to as microorganisms that colonize internal tissues of stems and yield endophytes producing pharmacologically important nat- leaves (Wilson 1995). More recent definitions denoted that they ural products hitherto only known from plants. are ubiquitous microorganisms present in virtually all plants In this review pharmaceutically valuable plant secondary on earth from the arctic to the tropics (Strobel and Daisy 2003; metabolites which were found to be produced by fungal endo- Huang et al. -
Role of Plant Derived Alkaloids and Their Mechanism in Neurodegenerative Disorders
Int. J. Biol. Sci. 2018, Vol. 14 341 Ivyspring International Publisher International Journal of Biological Sciences 2018; 14(3): 341-357. doi: 10.7150/ijbs.23247 Review Role of Plant Derived Alkaloids and Their Mechanism in Neurodegenerative Disorders Ghulam Hussain1,3, Azhar Rasul4,5, Haseeb Anwar3, Nimra Aziz3, Aroona Razzaq3, Wei Wei1,2, Muhammad Ali4, Jiang Li2, Xiaomeng Li1 1. The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China 2. Dental Hospital, Jilin University, Changchun 130021, China 3. Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, 38000 Pakistan 4. Department of Zoology, Faculty of Life Sciences, Government College University, Faisalabad, 38000 Pakistan 5. Chemical Biology Research Group, RIKEN Center for Sustainable Resource Science. 2-1 Hirosawa, Wako, Saitama 351-0198 Japan Corresponding authors: Professor Xiaomeng Li, The Key Laboratory of Molecular Epigenetics of Ministry of Education, Institute of Genetics and Cytology, Northeast Normal University, 5268 People's Street, Changchun, Jilin 130024, P.R. China. E-mail: [email protected] Tel: +86 186 86531019; Fax: +86 431 85579335 or Professor Jiang Li, Department of Prosthodontics, Dental Hospital, Jilin University, 1500 Tsinghua Road, Changchun, Jilin 130021, P.R. China. E-mail: [email protected] © Ivyspring International Publisher. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. Received: 2017.10.09; Accepted: 2017.12.18; Published: 2018.03.09 Abstract Neurodegenerative diseases are conventionally demarcated as disorders with selective loss of neurons.