DOCSLIB.ORG

University of Guelph Thesis Template

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
University of Guelph Thesis Template Plasma Pattern Recognition Receptors of Walleye (Sander vitreus M.) with an Emphasis on Mannose-binding Lectin-Like Protein and Viral Hemorrhagic Septicemia Virus by Mary Alexandra Reid A Thesis presented to The University of Guelph In partial fulfilment of requirements for the degree of Doctor of Philosophy in Pathobiology Guelph, Ontario, Canada © Mary Alexandra Reid, May, 2012 ABSTRACT PLASMA PATTERN RECOGNITION RECEPTORS OF WALLEYE (SANDER VITREUS M.) WITH AN EMPHASIS ON MANNOSE-BINDING LECTIN-LIKE PROTEIN AND VIRAL HEMORRHAGIC SEPTICEMIA VIRUS Alexandra Reid Advisor: University of Guelph, 2012 Professor John Lumsden Walleye (Sander vitreus M.) are valuable in commercial and recreational fisheries and are affected by bacterial, fungal and viral disease. Pattern recognition receptors (PRRs) are germline-encoded and constitutively expressed and bind non-self or altered- self for immune recognition. Walleye were hypothesised to have circulating PRRs that were capable of binding diverse pathogens. These PRRs were hypothesised to increase with infection, be distributed in immunologically relevant tissues and to be strain and age specific. PRR binding was measured by affinity chromatography, plasma binding assays, SDS-PAGE, Western blots, ELISA, PCR, and immunohistochemistry. ELISA and affinity chromatography assays were developed in rainbow trout (Oncorhynchus mykiss) with known PRRs. Trout ladderlectin was confirmed as a PRR binding viral hemorrhagic septicemia virus (VHSV). These techniques were adapted to walleye using Flavobacterium columnare, chitin, VHSV and Sepharose resin. A 22 kDa protein bound to F. columnare, a 17 kDa protein bound to chitin and a 34 kDa protein bound to VHSV were identified as similar to bass apolipoprotein, carp C3 and rainbow trout intelectin, respectively. PCR and 3'-RACE-PCR were used to generate nucleotide sequence to confirm identity of walleye apolipoprotein and mannose-binding lectin (MBL)-like protein from the intelectin-like sequence. Two rabbit polyclonal antibodies were raised to 34 and 67 kDa MBL amino acid sequences and used to verify MBL-like protein as a PRR for VHSV. Healthy walleye MBL-like protein plasma concentration was 7.5 ng/ml. Significant differences were found between geographically distant strains of walleye. An ELISA demonstrated that MBL-like protein had significant differences in binding affinity between multiple strains of VHSV and different viruses found in Ontario. MBL-like protein plasma levels increased with initial infection of naïve fish with waterborne and IP VHSV (107 pfu) but did not change with IP reinfection. Previous infection with VHSV significantly decreased walleye mortality. IHC of walleye shows MBL-like protein is distributed in epithelial surfaces, primarily skin, oropharynx, gill, gastrointestinal system, renal nephrons, connective tissue of gonads and plasma. There was no qualitative difference in MBL-like protein tissue distribution in healthy and VHSV-infected walleye. This is the first evidence for fish lectins binding viruses. ACKNOWLEDGEMENTS Mistahi ninanāskomāwak ēkwa nisākihāwak nināpēm, niwāhkōmākanak, nitōtēmak ēkwa niwītapimākanak. Kahkiyaw ōki nikī-pē-wīcihikwak mēkwāc kā- kiskinohamākawiyān. Namōya nika-kī-kaskihtān kīspin ēkāya kiyawāw ē-ohci- wīcihiyēk. Pocin, Maugre ēkwa Macaroni, tāpitawi. Thank you to my supervisor, Dr. John Lumsden and our technician, Mr. Paul Huber, as well as the rest of the FPL (past and present) for their assistance and support. I would like to thank the OVC community for frequent questions and help. I am grateful for the funding I have received from the Ontario Graduate Scholarship program and the OVC Fellowship program for providing financial assistance to allow me to complete my studies, as well as NSERC Strategic and Discovery grants and the Great Lakes Fisheries Commission for funding our VHSV research. iv DECLARATION OF WORK PERFORMED All work reported in this thesis was performed by myself under supervision of Dr. John Lumsden and my advisory committee; Dr. Brian Dixon, Dr. Claire Jardine, and Dr. Sarah Wootton, with following exceptions: Karrie Young assisted with the ladderlectin and intelectin ELISAs and in developing the rainbow trout – VHSV-Toyopearl assay in Chapter 2. Adrian Di Natale assisted with running many, many PCRs in Chapter 3. Dr. Jiaxi Wang at Queen's University conducted all mass spectrometry in Chapter 3. Mr. Nguyen Vo cultured the VHSV drum strain in the walleye WECF11f cell line for Chapter 4. Mr. Stephen Lord and Ms. Melinda Raymond from the Fish Health Laboratory, University of Guelph performed all virus isolations from experimental fish before and after infection in Chapter 4. Katrina Blackburn and the rest of the FPL assisted with evening checking of trial fish in Chapter 4. Dr. Stephen Reid, Mr. Tyler Flockhart and Mr. Leonard Shirose assisted in choosing appropriate statistical methods for Chapter 4. v TABLE OF CONTENTS Acknowledgements...............................................................................................................i Declaration of Work Performed ..........................................................................................ii Table of Contents................................................................................................................iii List of Tables.....................................................................................................................xix List of Figures ...................................................................................................................xx List of Abbreviations......................................................................................................xxiii Chapter One General Introduction............................................................................................................1 Literature Review.................................................................................................................2 1.1 Known Diseases of Walleye in Ontario, Canada...........................................................2 1.1.1 Columnaris in Walleye................................................................................2 1.1.2 Fungal and Metazoan Pathogens of Walleye..............................................3 1.1.3 Tumour-Forming Viral Pathogens of Walleye............................................4 1.1.4 Viral Hemorrhagic Septicemia Virus in Walleye........................................5 1.2 Pattern Recognition Receptors of Fish Involved in Innate Immune Recognition........7 vi 1.2.1 Epithelial Mucus-Associated Pattern Recognition Receptors....................8 1.2.2 Complement...............................................................................................9 1.2.3 Soluble Defense Lectins...........................................................................14 1.2.4 Natural Antibodies....................................................................................22 1.2.5 Toll-like Receptors...................................................................................23 1.2.6 Nucleotide-Binding Oligomerization Domain (NOD)-Like Receptors...26 1.2.7 RNA Helicases .........................................................................................27 1.2.8 Cellular Components................................................................................27 1.3 Interferons: Upregulators of Pattern Recognition Receptors in the Induced Antiviral Response............................................................................................................................30 1.3.1 Mx GTPases..................................................................................................32 1.3.2 ISG15............................................................................................................33 1.3.3 Protein Kinase R...........................................................................................33 1.3.4 Virus-Induced Gene 1...................................................................................34 1.3.5 Tumour Necrosis Factor α ...........................................................................35 1.4. Study Rationale..........................................................................................................36 Chapter Two Rainbow Trout Ladderlectin, but not Intelectin, Binds Viral Hemorrhagic Septicemia Virus IVb............................................................................................................................39 vii 2.1 Abstract........................................................................................................................39 2.2 Introduction.................................................................................................................40 2.3 Materials and Methods................................................................................................43 2.3.1 Plasma Binding Assays.............................................................................43 2.3.2 Indirect Enzyme-Linked Immunoassay (ELISA) for Lectin Binding to VHSV ..............................................................................................................44 2.3.3 VHSV-Conjugated Toyopearl Column Assay..........................................45 2.3.4 One-Dimensional Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (1-D SDS PAGE)....................................................................46 2.3.5 Slot Immunoblot.......................................................................................47
Load more

Recommended publications
  • Chautauqua County Envirothon Wildlife Review
    Chautauqua County Envirothon Wildlife Review • William Printup, Civil Engineering • Wendy Andersen, Permitting Allegheny National Forest Slide 1 Wildlife Learning Objectives For successful completion of the wildlife section, contestants should be able to: 1. Assess suitability of habitat for given wildlife species 2. Identify signs of wildlife 3. Cite examples of food chains based on specific site conditions 4. Analyze/Interpret site factors that limit or enhance population growth, both in the field and with aerial photos 5. Interpret significance of habitat alteration due to human impacts on site 6. Evaluate factors that might upset ecological balance of a specific site 7. Identify wildlife by their tracks, skulls, pelts, etc. 8. Interpret how presence of wildlife serves as an indicator of environmental quality 9. Identify common wildlife food Slide 2 WILDLIFE OUTLINE I. Identification of NYS Species (http://www.dec.ny.gov/23.html) • A. Identify NYS wildlife species by specimens, skins/pelts, pictures, skulls, silhouettes, decoys, wings, feathers, scats, tracks, animal sounds, or other common signs • B. Identify general food habits, habitats, and habits from teeth and/or skull morphology • C. Specific habitats of the above • II. Wildlife Ecology • A. Basic ecological concepts and terminology • B. Wildlife population dynamics • 1) Carrying capacity • 2) Limiting factors • C. Adaptations of wildlife • 1) Anatomical, physiological and/or behavioral • D. Biodiversity • 1) Genetic, species, ecosystem or community Slide 3 Outline Continued.. • III. Wildlife Conservation and Management • A. Common management practices and methods • 1) Conservation • 2) Protection • 3) Enhancement • B. Hunting regulations • C. Land conflicts with wildlife habitat needs • D. Factors influencing management decisions • 1) Ecological • 2) Financial •3) Social • E.
    [Show full text]
  • Evolutionary Genomics of a Plastic Life History Trait: Galaxias Maculatus Amphidromous and Resident Populations
    EVOLUTIONARY GENOMICS OF A PLASTIC LIFE HISTORY TRAIT: GALAXIAS MACULATUS AMPHIDROMOUS AND RESIDENT POPULATIONS by María Lisette Delgado Aquije Submitted in partial fulfilment of the requirements for the degree of Doctor of Philosophy at Dalhousie University Halifax, Nova Scotia August 2021 Dalhousie University is located in Mi'kma'ki, the ancestral and unceded territory of the Mi'kmaq. We are all Treaty people. © Copyright by María Lisette Delgado Aquije, 2021 I dedicate this work to my parents, María and José, my brothers JR and Eduardo for their unconditional love and support and for always encouraging me to pursue my dreams, and to my grandparents Victoria, Estela, Jesús, and Pepe whose example of perseverance and hard work allowed me to reach this point. ii TABLE OF CONTENTS LIST OF TABLES ............................................................................................................ vii LIST OF FIGURES ........................................................................................................... ix ABSTRACT ...................................................................................................................... xii LIST OF ABBREVIATION USED ................................................................................ xiii ACKNOWLEDGMENTS ................................................................................................ xv CHAPTER 1. INTRODUCTION ....................................................................................... 1 1.1 Galaxias maculatus ..................................................................................................
    [Show full text]
  • Fishes of Terengganu East Coast of Malay Peninsula, Malaysia Ii Iii
    i Fishes of Terengganu East coast of Malay Peninsula, Malaysia ii iii Edited by Mizuki Matsunuma, Hiroyuki Motomura, Keiichi Matsuura, Noor Azhar M. Shazili and Mohd Azmi Ambak Photographed by Masatoshi Meguro and Mizuki Matsunuma iv Copy Right © 2011 by the National Museum of Nature and Science, Universiti Malaysia Terengganu and Kagoshima University Museum All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any means without prior written permission from the publisher. Copyrights of the specimen photographs are held by the Kagoshima Uni- versity Museum. For bibliographic purposes this book should be cited as follows: Matsunuma, M., H. Motomura, K. Matsuura, N. A. M. Shazili and M. A. Ambak (eds.). 2011 (Nov.). Fishes of Terengganu – east coast of Malay Peninsula, Malaysia. National Museum of Nature and Science, Universiti Malaysia Terengganu and Kagoshima University Museum, ix + 251 pages. ISBN 978-4-87803-036-9 Corresponding editor: Hiroyuki Motomura (e-mail: [email protected]) v Preface Tropical seas in Southeast Asian countries are well known for their rich fish diversity found in various environments such as beautiful coral reefs, mud flats, sandy beaches, mangroves, and estuaries around river mouths. The South China Sea is a major water body containing a large and diverse fish fauna. However, many areas of the South China Sea, particularly in Malaysia and Vietnam, have been poorly studied in terms of fish taxonomy and diversity. Local fish scientists and students have frequently faced difficulty when try- ing to identify fishes in their home countries. During the International Training Program of the Japan Society for Promotion of Science (ITP of JSPS), two graduate students of Kagoshima University, Mr.
    [Show full text]
  • A New Species of Larimichthys from Terengganu, East Coast of Peninsular Malaysia (Perciformes: Sciaenidae)
    Zootaxa 3956 (2): 271–280 ISSN 1175-5326 (print edition) www.mapress.com/zootaxa/ Article ZOOTAXA Copyright © 2015 Magnolia Press ISSN 1175-5334 (online edition) http://dx.doi.org/10.11646/zootaxa.3956.2.7 http://zoobank.org/urn:lsid:zoobank.org:pub:28EA3933-85D8-401B-831E-F5165B4172E4 A new species of Larimichthys from Terengganu, east coast of Peninsular Malaysia (Perciformes: Sciaenidae) YING GIAT SEAH1,2,4,6, NORHAFIZ HANAFI1, ABD GHAFFAR MAZLAN3,4 & NING LABBISH CHAO5 1School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia. E-mail: [email protected] 2Fish Division, South China Sea Repository and Reference Center, Institute of Oceanography and Environment, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia 3Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia 4Marine Ecosystem Research Center, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia. 5National Museum of Marine Biology and Aquarium, 2 Houwan Road, Checheng, Pingtung, 944, Taiwan, Republic of China 6Corresponding author Abstract A new species of Larimichthys from Terengganu, east coast of Peninsular Malaysia is described from specimens collected from the fish landing port at Pulau Kambing, Kuala Terengganu. Larimichthys terengganui can be readily distinguished from other species of the genus by having an equally short pair of ventral limbs at the end of the gas bladder appendages, which do not extend lateral-ventrally to the lower half of the body wall, and fewer dorsal soft rays (29–32 vs. 31–36) and vertebrae (24 vs. 25–28). Larimichthys terengganui can be distinguished from L.
    [Show full text]
  • Department of the Interior
    Vol. 77 Monday, No. 63 April 2, 2012 Part II Department of the Interior Fish and Wildlife Service 50 CFR Part 17 Endangered and Threatened Wildlife and Plants; 12-Month Finding on a Petition to List the San Francisco Bay-Delta Population of the Longfin Smelt as Endangered or Threatened; Proposed Rule VerDate Mar<15>2010 19:41 Mar 30, 2012 Jkt 226001 PO 00000 Frm 00001 Fmt 4717 Sfmt 4717 E:\FR\FM\02APP2.SGM 02APP2 mstockstill on DSK4VPTVN1PROD with PROPOSALS2 19756 Federal Register / Vol. 77, No. 63 / Monday, April 2, 2012 / Proposed Rules DEPARTMENT OF THE INTERIOR FOR FURTHER INFORMATION CONTACT: Furthermore, we found the Sacramento- Mike Chotkowski, Field Supervisor, San San Joaquin River estuary population of Fish and Wildlife Service Francisco Bay-Delta Fish and Wildlife longfin smelt was not a distinct Office (see ADDRESSES); by telephone at population segment (DPS) because we 50 CFR Part 17 916–930–5603; or by facsimile at 916– determined that the population was not 930–5654 mailto:. If you use a biologically significant to the species as [Docket No. FWS–R8–ES–2008–0045: telecommunications device for the deaf a whole, and did not appear to be 4500030113] (TDD), please call the Federal sufficiently reproductively isolated. Endangered and Threatened Wildlife Information Relay Service (FIRS) at On August 8, 2007, we received a and Plants; 12-Month Finding on a 800–877–8339. petition from the Bay Institute, the Petition to List the San Francisco Bay- SUPPLEMENTARY INFORMATION: Center for Biological Diversity, and the Delta Population of the Longfin Smelt Background Natural Resources Defense Council to as Endangered or Threatened list the San Francisco Bay-Delta Section 4(b)(3)(B) of the Endangered (hereafter referred to as the Bay-Delta) AGENCY: Fish and Wildlife Service, Species Act of 1973, as amended (Act) population of the longfin smelt as a DPS Interior.
    [Show full text]
  • Download (3MB)
    MAGYAR NYELVŐR 142. ÉVF. * 2018. ÁPRILIS–JÚNIUS * 2. SZÁM Dayka Gábornak Aorthographia címet kapott, kéziratban fennmaradt magyar nyelvű, nyelvészeti jellegű írásáról 1. Bevezetés. A tervem az, hogy a nyelvtudomány-történet keretében bemutas- sam a mindössze huszonnyolc évet élt, sajátos hangú 18. századi költőnek, Dayka Gábornak a kéziratban fennmaradt nyelvtani, egyáltalán nyelvészeti irányultsá- gú munkáit. A sorrendet illetően a Dayka-kutatáshoz alapot nyújtó Dayka Gábor Összes Művei című kötetet követem (az adatait l. DGÖM). Ez a kötet egyébként tartalmazza Dayka Gábornak – költői és prózai alkotásain kívül – a következő nyelvtani, nyelvészeti jellegű írásait: „Grammatikák és grammatikai jegyzetek: 1. Magyar nyelvű grammatikai tárgyú írások: a) Aorthographia és b) Grammati- kai feljegyzések. – 2. Proludium in Institutiones Linguae Hungaricae. – 3. Ternio Grammaticae Hungaricae 1794” (DGÖM 7). Meg kell jegyeznem, hogy bár e munkáknak se a pontos keletkezési idejét, se egymás utáni következésük sor- rendjét nem lehetett bizonyítottan megállapítani, a DGÖM-ben alkalmazott és idézett sorrend látszik logikusnak és követendőnek. A kötet tartalmazza az ere- deti szövegeken kívül – ha idegen nyelvűek – azok fordítását is, továbbá minden szöveg után a széles körű rálátással megírt jegyzeteket, amelyek megemlékeztek a kéziratról, az illető szöveg megjelenéséről, valamint közölnek szövegkritikát, és „Magyarázatok” címen tárgyalják az illető mű keletkezés- és fogadástörténetét, valamint közölnek számos fogalmat, jelenséget megvilágító megjegyzést. Egyéb- ként a filológusi feldolgozást a lehetséges teljességre, a rendszerességre, a minden lényeges kérdésnek az utánajárására, a logikus felépítésre, a pontosságra, valamint a világos megfogalmazásra való törekvés jellemzi (l. részletesen: DGÖM 64–9). Elöljáróban még szólok több éve tartó Dayka-kutatásaim első eredményé- ről, a Mi vitte Dayka Gábort, a költőt a nyelvtanírás, egyáltalán a nyelvtudomány felé? című tanulmányról (Szathmári 2016).
    [Show full text]
  • Direct Identification of Fish Species by Surface Molecular Transferring
    Electronic Supplementary Material (ESI) for Analyst. This journal is © The Royal Society of Chemistry 2020 Supplementary materials for Direct Identification of Fish Species by Surface Molecular Transferring Mingke Shao, Hongyan Bi* College of Food Science and Engineering, Shanghai Ocean University, Hucheng Ring Road 999, Pudong New District, 201306 Shanghai, China * To whom correspondence should be addressed. E-mail address: [email protected] E-mail address for the other authors: [email protected] S1 S1. Photos and information on the analyzed fish samples Fig. S1. Photos of fishes analyzed in the present study: (A) Oreochromis mossambicus (B) Epinephelus rivulatus (C) Mugil cephalus; (D) Zeus faber (E) Trachinotus ovatus (F) Brama japonica (G) Larimichthys crocea (H) Larimichthys polyactis (I) Pampus argenteus. Scale bar in each photo represents 1 cm. Table S1. List of the scientific classification of fishes analyzed in the study. The classification of fishes refers to https://www.fishbase.de/. Binomial Abbreviatio English Chinese name n common Scientific classification name (Scientific name name) Actinopterygii (class) > Perciformes (order) > Japanese Brama Brama BJ Bramidae (family) > Wufang japonica japonica Brama (genus) > B. brama (species) Actinopterygii (class) > Silver Scombriformes(order) > Baichang pomfret; Pampus PA ( Fish White argenteus Stromateidae family) > pomfret Pampus (genus) > P. argenteus (species) Haifang Zeus faber Actinopterygii (class) > (commonly Linnaeu; Zeus faber ZF Zeiformes (order) > called: John Dory; Zeidae (family) > S2 Yueliang target perch Zeus (genus) > Fish) Z. faber (species) Actinopteri (class) > OM Cichliformes (order) > Mozambique Oreochromis Cichlidae (family) > Luofei Fish tilapia mossambicus Oreochromis (genus) > O.mossambicus (species) Actinopterygii (class) > MC Mugiliformes (order) Xiaozhai Flathead Mugil Mugilidae (family) > Fish grey mullet cephalus Mugil (genus) > M.
    [Show full text]
  • Greater Annual Macrophyte Abundance in Warmer Locations in Consequence of Nutrients in Lakes V205a Page 2 the Longer Plant Growing-Season
    Nutrients in Lakes V205a Page 1 Reference Type: Journal Article Record Number: 482 Author: Moss, B.; Stephen, D.; Balayla, D. M.; Becares, E.; Collings, S. E.; Fernandez-Alaez, C.; Fernandez-Alaez, M.; Ferriol, C.; Garcia, P.; Goma, J.; Gyllstroem, M.; Hansson, L. A.; Hietala, J.; Kairesalo, T.; Miracle, M. R.; Romo, S.; Rueda, J.; Russell, V.; Stahl-Delbanco, A.; Svensson, M.; Vakkilainen, K.; Valentin, M.; Van De Bund, W. J.; Van Donk, E.; Vicente, E.; Villena, M. J. Year: 2004 Title: Continental-scale patterns of nutrient and fish effects on shallow lakes: Synthesis of a pan-european mesocosm experiment Journal: Freshwater Biology Volume: 49 Issue: 12 Pages: 1633-1649 Accession Number: AN 2005:18924 Abstract: 1. Results are analyzed from 11 expts. in which effects of fish addn. and nutrient loading on shallow lakes were studied in mesocosms. The expts., five in 1998, six in 1999, were carried out in six lakes, distributed from Finland to southern Spain, according to a std. protocol. 2. Effects of the treatments on 29 std. chem., phytoplankton and zooplankton variables are examd. to assess the relative importance of bottom-up (nutrient enrichment) and top-down (fish predation) effects. For each year, the expts. in different locations are treated as replicates in a meta-anal. Results of individual expts. are then compared in terms of the patterns of significant influences of nutrient addn. and fish predation with these overall results (the baseline), and between years in the same location. 3. The overall meta-anal. gave consistent results across the 2 years, with nutrient loading influencing all of the chem.
    [Show full text]
  • The Genome of Mekong Tiger Perch (Datnioides Undecimradiatus) Provides 3 Insights Into the Phylogenic Position of Lobotiformes and Biological Conservation
    bioRxiv preprint doi: https://doi.org/10.1101/787077; this version posted September 30, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Article 2 The genome of Mekong tiger perch (Datnioides undecimradiatus) provides 3 insights into the phylogenic position of Lobotiformes and biological conservation 4 5 Shuai Sun1,2,3†, Yue Wang1,2,3,†, Xiao Du1,2,3,†, Lei Li1,2,3,4,†, Xiaoning Hong1,2,3,4, Xiaoyun Huang1,2,3, He Zhang1,2,3, 6 Mengqi Zhang1,2,3, Guangyi Fan1,2,3, Xin Liu1,2,3,*, Shanshan Liu1,2,3* 7 8 1 BGI-Qingdao, BGI-Shenzhen, Qingdao, 266555, China 9 2 BGI-Shenzhen, Shenzhen, 518083, China 10 3 China National GeneBank, BGI-Shenzhen, Shenzhen, 518120, China 11 4 School of Future Technology, University of Chinese Academy of Sciences, Beijing 101408, China 12 13 14 † These authors contributed equally to this work. 15 * Correspondence authors: [email protected] (S. L.), [email protected] (X. L.) 16 17 Abstract 18 Mekong tiger perch (Datnioides undecimradiatus) is one ornamental fish and a 19 vulnerable species, which belongs to order Lobotiformes. Here, we report a ~595 Mb 20 D. undecimradiatus genome, which is the first whole genome sequence in the order 21 Lobotiformes. Based on this genome, the phylogenetic tree analysis suggested that 22 Lobotiformes and Sciaenidae are closer than Tetraodontiformes, resolving a long-time 23 dispute.
    [Show full text]
  • A Cyprinid Fish
    DFO - Library / MPO - Bibliotheque 01005886 c.i FISHERIES RESEARCH BOARD OF CANADA Biological Station, Nanaimo, B.C. Circular No. 65 RUSSIAN-ENGLISH GLOSSARY OF NAMES OF AQUATIC ORGANISMS AND OTHER BIOLOGICAL AND RELATED TERMS Compiled by W. E. Ricker Fisheries Research Board of Canada Nanaimo, B.C. August, 1962 FISHERIES RESEARCH BOARD OF CANADA Biological Station, Nanaimo, B0C. Circular No. 65 9^ RUSSIAN-ENGLISH GLOSSARY OF NAMES OF AQUATIC ORGANISMS AND OTHER BIOLOGICAL AND RELATED TERMS ^5, Compiled by W. E. Ricker Fisheries Research Board of Canada Nanaimo, B.C. August, 1962 FOREWORD This short Russian-English glossary is meant to be of assistance in translating scientific articles in the fields of aquatic biology and the study of fishes and fisheries. j^ Definitions have been obtained from a variety of sources. For the names of fishes, the text volume of "Commercial Fishes of the USSR" provided English equivalents of many Russian names. Others were found in Berg's "Freshwater Fishes", and in works by Nikolsky (1954), Galkin (1958), Borisov and Ovsiannikov (1958), Martinsen (1959), and others. The kinds of fishes most emphasized are the larger species, especially those which are of importance as food fishes in the USSR, hence likely to be encountered in routine translating. However, names of a number of important commercial species in other parts of the world have been taken from Martinsen's list. For species for which no recognized English name was discovered, I have usually given either a transliteration or a translation of the Russian name; these are put in quotation marks to distinguish them from recognized English names.
    [Show full text]
  • Alternative Methods for Assessing Habitat Quality in Freshwater Systems
    Alternative methods for assessing habitat quality in freshwater systems Item Type Thesis or dissertation Authors Mauvisseau, Quentin Citation Mauvisseau, Q 2020, 'Alternative methods for assessing habitat quality in freshwater systems', PhD thesis, University of Derby, Derby Publisher University of Derby Download date 28/09/2021 22:40:52 Link to Item http://hdl.handle.net/10545/625067 University of Derby Alternative methods for assessing habitat quality in freshwater systems Quentin Mauvisseau Doctor of Philosophy 2020 THIS PAGE HAS BEEN INTENTIONALLY LEFT BLANK 2 CONTENTS Figures............................................................................................................................................. 7 Tables .............................................................................................................................................. 9 Appendices .................................................................................................................................... 11 Abbreviations ................................................................................................................................ 12 Preface........................................................................................................................................... 14 Abstract ......................................................................................................................................... 17 Acknowledgment .........................................................................................................................
    [Show full text]
  • The Genome of Mekong Tiger Perch
    www.nature.com/scientificreports OPEN The genome of Mekong tiger perch (Datnioides undecimradiatus) provides insights into the phylogenetic position of Lobotiformes and biological conservation Shuai Sun1,2,3,7, Yue Wang1,2,3,7, Wenhong Zeng4,7, Xiao Du1,2,3,7, Lei Li1,2,3,5,7, Xiaoning Hong1,2,3,6, Xiaoyun Huang1,2,3, He Zhang1,2,3, Mengqi Zhang1,2,3, Guangyi Fan1,2,3, Xin Liu1,2,3 ✉ & Shanshan Liu1,2,3 ✉ Mekong tiger perch (Datnioides undecimradiatus) is an ornamental and vulnerable freshwater fsh native to the Mekong basin in Indochina, belonging to the order Lobotiformes. Here, we generated 121X stLFR co-barcode clean reads and 18X Oxford Nanopore MinION reads and obtained a 595 Mb Mekong tiger perch genome, which is the frst whole genome sequence in the order Lobotiformes. Based on this genome, the phylogenetic tree analysis suggested that Lobotiformes is more closely related to Sciaenidae than to Tetraodontiformes, resolving a long-time dispute. We depicted the genes involved in pigment development in Mekong tiger perch and results confrmed that the four rate-limiting genes of pigment synthesis had been retained after fsh-specifc genome duplication. We also estimated the demographic history of Mekong tiger perch, which showed that the efective population size sufered a continuous reduction possibly related to the contraction of immune-related genes. Our study provided a reference genome resource for the Lobotiformes, as well as insights into the phylogenetic position of Lobotiformes and biological conservation. Mekong tiger perch (Datnioides undecimradiatus) is one tropical freshwater fish, belonging to the order Lobotiformes under series Eupercaria1.
    [Show full text]