DOCSLIB.ORG

Program and Abstracts

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Program and Abstracts 1 48th ANNUAL DROSOPHILA RESEARCH CONFERENCE March 7-11, 2007 Philadelphia Marriott Downtown Philadelphia, Pennsylvania Program and Abstracts • 2007 Meeting Organizers Steve DiNardo, University of Pennsylvania School of Medicine Liz Gavis, Princeton University Tom Jongens, University of Pennsylvania School of Medicine Jessica Treisman, NYU Medical Center • 2007 Drosophila Board of Directors Officers and Regional Representatives President Trudy MacKay North Carolina State University President-Elect Utpal Banerjee University of California at Los Angeles Past-President Mark Krasnow Stanford University Medical School Past-President & Elections Chair Lynn Cooley Yale University School of Medicine Past-President Ruth Lehman New York University School of Medicine Treasurer Michael Bender University of Georgia Canada Howard Lipshitz University of Toronto Great Lakes Amanda Simcox Ohio State University Northwest Barb Taylor Oregon State University College of Science Southeast Rebecca Kellum University of Kentucky California Ken Burtis University of California, Davis Heartland Susan Abmayr Stowers Institute for Medical Research New England Mitzi Kuroda Harvard University Medical School Mid-Atlantic Claude Desplan New York University Midwest Pam Geyer University of Iowa • International Representatives Australia/Oceana Robert Saint Australian National University Asia Yasushi Hiromi National Institute of Genetics Europe David Ish-Horowicz Cancer Research UK • Sponsored by The Genetics Society of America 9650 Rockville Pike, Bethesda, MD 20814-3998 Telephone: (301) 634-7300 fax: (301) 634-7079 e-mail: [email protected] Web site: http://www.genetics-gsa.org/ Conference site: http://www.drosophila-conf.org/ 2 3 TABLE OF CONTENTS Schedule of Events .................................................................................................................................................... 4 Floorplans Philadelphia Marriott Downtown – Levels Four and Five, Meeting Space ......................................... 11 Philadelphia Marriott Downtown – Level Four, Posters and Exhibition Hall ....................................... 12 General Information ................................................................................................................................................. 13 Larry Sandler Memorial Lecture ............................................................................................................................ 15 The DeLill Nasser Award for Professional Development in Genetics................................................................ 15 Conversations in Genetics, Volume III .................................................................................................................... 16 Guidelines for Making Platform Presentations ...................................................................................................... 17 Guidelines for Making Poster Presentations ......................................................................................................... 18 Exhibitors ................................................................................................................................................................. 19 Opening General Session and Plenary Sessions I and II ...................................................................................... 21 Workshops ................................................................................................................................................................. 22 Platform Sessions..................................................................................................................................................... 26 Poster Sessions ........................................................................................................................................................ 37 Abstracts Introduction ............................................................................................................................................. 77 Platform Sessions ................................................................................................................................... 79 Poster Sessions .................................................................................................................................... 142 Speaker and Author Index ...................................................................................................................................... 391 Keyword Index ......................................................................................................................................................... 407 FlyBase Genetic Index to Abstracts ..................................................................................................................... 415 FUTURE CONFERENCE DATES 2008 2011 April 2-6 March 30-April 3 San Diego, California San Diego, California 2009 2012 March 4-8 March 7-11 Chicago, Illinois Chicago, Illinois 2010 2013 April 7-11 April 3-7 Washington, DC Washington, DC 4 SCHEDULE OF EVENTS 48th Annual Drosophila Research Conference WEDNESDAY, MARCH 7 Time Event Location 12:00 noon - 6:00 pm Ecdysone Workshop Grand Ballroom Organizers: Robert Ward, University of Kansas, Salons A-D Lawrence; and Craig Woodward, Mount Holyoke College, Level Five South Hadley, Massachusetts 3:00 pm - 6:00 pm Drosophila Board of Directors Meeting Room 407-409 Level Four 3:30 pm - 9:00 pm Registration & Book Pickup Franklin Hall Foyer Level Four 7:00 pm - 9:00 pm Opening General Session Grand Ballroom Moderator: Steve DiNardo, University of Pennsylvania Salons E-H Medical School, Philadelphia Level Five 7:00 pm Welcome and Opening Remarks Steve DiNardo, University of Pennsylvania Medical School, Philadelphia 7:10 pm Introduction of Historical Address Speaker Mark Fortini, National Cancer Institute, Frederick, Maryland 7:20 pm Historical Address Spyros Artavanis-Tsakonas, Harvard Medical School/MGH Cancer Center, Boston, Massachusetts 8:10 pm Introduction of Larry Sandler Memorial Lecture Helen Salz, Case Western Reserve University, Cleveland, Ohio 9:00 pm - 12:00 am Mixer/Reception Grand Ballroom Salons A-D and Foyer Level Five THURSDAY, MARCH 8 7:15 am - 8:30 am Continental Breakfast Grand Ballroom Foyer Level Five 8:00 am - 5:00 pm Registration & Book Pickup Franklin Hall Foyer Level Four 5 Time Event Location 8:30 am - 12:00 noon Plenary Session I Grand Ballroom Moderator: Liz Gavis, Princeton University, New Jersey Salons E-H Level Five 8:30 am Image Award Presentation Liz Gavis, Princeton University, New Jersey 8:35 am Circadian Clocks and Sleep Ravi Allada, Northwestern University, Evanston, Illinois 9:00 am Membrane and Organelle Traffic in Neurons Thomas Schwarz, Children's Hospital, Boston, Massachusetts 9:30 am Taste Recognition in Drosophila Kristin Scott, University of Iowa, Iowa City 10:00 am Break 10:30 am Regulation of Cell Surface Mechanics Underlying Tissue Morphogenesis Thomas Lecuit, IBDM, Marseille Cedex, France 11:00 am Progressive Heterochromatin: Players and Pathways Lori Wallrath, University of Iowa, Iowa City 11:30 am Mechanisms of P Element Transposition and Alternative Pre-mRNA Splicing Donald Rio, University of California, Berkeley 1:00 pm - 5:00 pm FlyMine Drop-in Demonstrations Room 414-415 Presentation at 2:15 pm Level Four 1:00 pm - 6:00 pm FlyBase Demonstrations Room 401-403 Demo Room Open for Tutorials and Discussions Level Four 1:30 pm-2:00 pm Presentations: Navigating the new FlyBase website. Accessing the dozen fly genomes. Increasing your query power: TermLink & QueryBuilder. 3:00 pm-3:30 pm Presentations: Navigating the new FlyBase website. Accessing the dozen fly genomes. Increasing your query power: TermLink & QueryBuilder. 2:00 pm - 4:00 pm Exhibits & Poster Viewing Franklin Hall 2:00 pm-3:00 pm: Even-Numbered Poster Authors Level Four 3:00 pm-4:00 pm: Odd-Numbered Poster Authors 4:30 pm - 6:30 pm Concurrent Platform Sessions I Immune System and Cell Death Grand Ballroom Salon E Level Five 6 Time Event Location Organogenesis Grand Ballroom Salons H-J Level Five Neurophysiology and Behavior Grand Ballroom Salons G, K-L Level Five 8:00 pm - 11:00 pm Exhibits & Poster Viewing Franklin Hall 8:00 pm-9:00 pm: "A" Poster Authors Level Four 9:00 pm-10:00 pm: "B" Poster Authors 10:00 pm-11:00 pm: "C" Poster Authors FRIDAY, MARCH 9 8:30 am - 5:00 pm Registration & Book Pickup Franklin Hall Foyer Level Four 8:30 am - 10:15 am Concurrent Platform Sessions II Evolution and Quantitative Genetics Grand Ballroom Salon E Level Five Regulation of Gene Expression Grand Ballroom Salons H-J Level Five Cytoskeleton and Cell Biology Grand Ballroom Salons G, K-L Level Five 9:00 am - 5:00 pm FlyMine Drop-in Demonstrations Room 414-415 Presentations at 10:30 am and 2:15 pm Level Four 9:00 am - 6:00 pm FlyBase Demonstrations Room 401-403 Demo Room Open for Tutorials and Discussions Level Four 3:00 pm-3:30 pm Presentations: Navigating the new FlyBase website. Accessing the dozen fly genomes. Increasing your query power: TermLink & QueryBuilder. 10:15 am - 10:45 am Break Grand Ballroom Foyer Level Five 10:45 am - 12:30 pm Concurrent Platform Sessions III Evolution and Quantitative Genetics Grand Ballroom Salon E Level Five 7 Time Event Location Regulation of Gene Expression Grand Ballroom Salons H-J Level Five Cytoskeleton and Cell Biology Grand Ballroom Salons G, K-L Level Five 12:45 pm - 1:45 pm GSA Mentor Roundtable Room 413 Limited attendance. Sign up by 5:00 pm on March
Load more

Recommended publications
  • Columbia College Columbia University in the City of New York
    Columbia College Columbia University in the City of New York BULLETIN | 2011–2012 JULY 15, 2011 Directory of Services University Information (212) 854-1754 Columbia College On-Line http://www.college.columbia.edu/ ADDRESS INQUIRIES AS FOLLOWS: Financial Aid: Office of Financial Aid and Educational Financing Office of the Dean: Mailing address: Columbia College 100 Hamilton Hall 208 Hamilton Hall Mail Code 2802 Mail Code 2805 1130 Amersterdam Avenue 1130 Amersterdam Avenue New York, NY 10027 New York, NY 10027 Office location: 407 Alfred Lerner Hall telephone (212) 854-2441 telephone (212) 854-3711 Academic Success Programs (HEOP/NOP): Health Services: 403 Alfred Lerner Hall Health Services at Columbia Mail Code 2607 401 John Jay Hall 2920 Broadway Mail Code 3601 New York, NY 10027 519 West 114th Street telephone (212) 854-3514 New York, NY 10027 telephone (212) 854-7210 Admissions: http://www.health.columbia.edu/ Office of Undergraduate Admissions 212 Hamilton Hall Housing on Campus: Mail Code 2807 Residence Halls Assignment Office 1130 Amsterdam Avenue 111 Wallach Hall New York, NY 10027 Mail Code 4202 telephone (212) 854-2522 1116 Amsterdam Avenue http://www.studentaffairs.columbia.edu/admissions/ New York, NY 10027 (First-year, transfer, and visitor applications) telephone (212) 854-2775 http://www.columbia.edu/cu/reshalls/ Dining Services: 103 Wein Hall Housing off Campus: Mail Code 3701 Off-Campus Housing Assistance 411 West 116th Street 419 West 119th Street New York, NY 10027 New York, NY 10027 telephone (212) 854-6536 telephone
    [Show full text]
  • Mccmb ’09 Ttcggtgatatccaggcggcgggcaatcatctt
    TCAGAAAATGCGCTCCTGATGCACCCATACCGC TGCTTCCACGCGAGACTTGAGCTTCATTTTCTT CAGCATGTGCTTGACGTGCACTTTTACTGTGCT TTCGGTGATATCCAGGCGGCGGGCAATCATCTT GTTCGGCAAACCCTGGGCAATCAGCTTGAGAAT ATCGCGCTCGCGTGGGGTTAACTGGTTAACATC TCAGAAAATGCGCTCCTGATGCACCCATACCGC TGCTTCCACGCGAGACTTGAGCTTCATTTTCTT CAGCATGTGCTTGACGTGCACTTTTACTGTGCT MCCMB ’09 TTCGGTGATATCCAGGCGGCGGGCAATCATCTT POCEEDINGSR OF THE IN TERNATIONAL MSCOWCNFERENCEOO ONCMPUTATIONALO MLECULARBOLOGYOI July 20-23, 2009 Moscow, Russia Organizers ЕР И И Е Н И Ж Б Н И И О О И И Н Б Ф DepartmentofBioengineeringandBioinformatics О Т Р Е М Т Ь А Л ofM.V.LomonosovMoscowStateUniversity Т У И К К А И Ф 1930 BiologicalDepartment ofM.V.LomonosovMoscowStateUniversity У StateScientificCentreGosNIIGenetika Institutefor Information TrasnsmissionProblems,RAS The ScientificCouncil onBiophysicsRAS, EngelhardtInstituteofMolecular Biology Russian AcademyofSciences Sponsoredby Р И RussianFundofBasicResearch INRIA,France INRIA theFrenchNationalInstituteforResearch inComputerScienceandControl DepartmentofBioengineeringandBioinformatics ofM.V.LomonosovMoscowStateUniversity BiologicalDepartmentofM.V.LomonosovMoscowStateUniversity StateScientificCentreGosNIIGenetika InstituteforInformationTrasnsmissionProblems,Russian A cademyof S ciences TheScientificCouncilonBiophysics , RAS ussian cademyof ciences EngelhardtInstituteofMolecularBiology,Russian AcademyofSciences with financial support of RussianFundofBasicResearch INRIA,France (theFrenchNationalInstituteforResearchinComputerScienceandControl) POCEEDINGSR MCCMB ’09 Moscow, Russia July
    [Show full text]
  • Download Program Guide
    2011 C. elegans Meeting Organizing Committee Co-chairs: Oliver Hobert Columbia University Meera Sundaram University of Pennsylvania Organizing Committee: Raffi Aroian University of California, San Diego Ikue Mori Nagoya University Jean-Louis Bessereau INSERM Benjamin Podbilewicz Technion Israel Institute of Keith Blackwell Harvard Medical School Technology Andrew Chisholm University of California, San Diego Valerie Reinke Yale University Barbara Conradt Dartmouth Medical School Janet Richmond University of Illinois, Chicago Marie Anne Felix CNRS-Institut Jacques Monod Ann Rougvie University of Minnesota David Greenstein University of Minnesota Shai Shaham Rockefeller University Alla Grishok Columbia University Ahna Skop University of Wisconsin, Madison Craig Hunter Harvard University Ralf Sommer Max-Planck Institute for Bill Kelly Emory University Developmental Biology, Tuebingen Ed Kipreos University of Georgia Asako Sugimoto RIKEN, Kobe Todd Lamitina University of Pennsylvania Heidi Tissenbaum University of Massachusetts Chris Li City College of New York Medical School Sponsored by The Genetics Society of America 9650 Rockville Pike, Bethesda, MD 20814-3998 telephone: (301) 634-7300 fax: (301) 634-7079 e-mail: [email protected] Web site: http:/www.genetics-gsa.org Front cover design courtesy of Ahna Skop 1 Table of Contents Schedule of All Events.....................................................................................................................4 Maps University of California, Los Angeles, Campus .....................................................................7
    [Show full text]
  • The Destinies and Destinations Meeting Review of Rnas
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Cell, Vol. 95, 451±460, November 13, 1998, Copyright 1998 by Cell Press The Destinies and Destinations Meeting Review of RNAs Tulle Hazelrigg GMC. Since Prospero is independently localized to the Department of Biological Sciences GMC, is prospero mRNA localization gratuitous? The Columbia University answer is no. While staufen mutants alone are not defec- New York, New York 10027 tive in GMC differentation, staufen is important for GMC fate, since staufen mutations enhance defects in GMC fate caused by hypomorphic prospero alleles. Thus, this The third biennial FASEB Summer Research Confer- binary cell fate decision appears to be controlled redun- ence, ªIntracellular RNA Sorting, Transport, and Local- dantly by localization of both prospero mRNA and Pros- ization,º was held June 6±11 in Snowmass, Colorado. pero to the GMC daughter cell. Topics included the biological functions of localized Early Embryonic Development RNAs, the nature of nuclear±cytoplasmic RNA transport, Several mRNAs are localized to the animal or vegetal the role of signaling pathways in RNA localization, the poles of the Xenopus oocyte, and some are implicated nature of cis-acting localization elements within RNAs, in axial patterning of the embryo (reviewed in Schnapp the proteins that bind these elements, and the cellular et al., 1997). Mary Lou King (University of Miami Medical mechanisms that achieve cytoplasmic transport and an- School) presented definitive evidence for an essential choring of RNAs to specific domains within cells. role of one vegetally localized mRNA, VegT mRNA, in early embryogenesis.
    [Show full text]
  • One Hundred and First
    COneO mm Hundred ENC andEM FirstENT SUNDAY, THE NINTEENTH OF MAY, TWO THOUSAND NINETEEN COMMENCEMENT PROGRAM PROCESSIONAL ... Selected Marches . The Manchester Pipe Band Daniel Pisowloski, Pipe Major Gordon Bell, Drum Sergeant CALL TO ORDER . Marc R. Forster Henry B. Plant Professor of History and College Marshal A CALL TO COMMUNITY . Angela Nzegwu Interim Director of Religious and Spiritual Programs AMERICA THE BEAUTIFUL ��������������������������������������������������������������������������������������������������� George Samuel Grotheer ’19 Constitution Brass Quintet REMARKS BY THE PRESIDENT . Katherine Bergeron President of the College SENIOR CLASS SPEAKER . Issraa Omayma Faiz ’19 PRESENTATION OF OAKES AND LOUISE AMES PRIZE . Jeffrey Cole Dean of the Faculty Professor of Anthropology PRESENTATION OF ANNA LORD STRAUSS MEDAL ���������������������������������������������������������������������� Jefferson A. Singer Dean of the College Faulk Foundation Professor of Psychology CONFERRING OF HONORARY DEGREES . President Bergeron Dean Jeff Cole DeFred G. Folts III ’82 Chair, Connecticut College Board of Trustees COMMENCEMENT ADDRESS . Martin Chalfie and Tulle Inger Hazelrigg CONFERRING OF DEGREES ���������������������������������������������������������������������������������������������������������������� President Bergeron Dean Singer Marina J. Melendez Associate Dean of the College; Dean for Juniors, Seniors and Transfers; Posse Coordinator Marc R. Forster ALUMNI ASSOCIATION WELCOME . Jamie Glanton Costello ’89 President, Board of
    [Show full text]
  • ECWM 2000 Abstracts Platform Sessions WHSCAB Auditorium (10 Min
    East Coast Worm Meeting 2000 ECWM 2000 Abstracts Platform Sessions WHSCAB Auditorium (10 min. presentation + 2 min. Q&A) Friday, June 9 7:00-8:48 p.m., Session 1 Andy Singson, Chair Two new cell-death genes and cell-corpse engulfment genes both contribute to cell killing Peter Reddien, Scott Cameron, Michael Huang, Bob Horvitz The cell-corpse engulfment protein CED-1 is a transmembrane receptor that recognizes cell corpses Zheng Zhou, Bob Horvitz Testing Functions of Phagocytosis Receptor Homologs in Cell Corpse Elimination and Gonadal Outgrowth Sambath Chung, Monica Driscoll Components of the cytoskeleton, involved in the biogenesis of membrane channels, interfere with mec-4(d)-induced cell death. Nektarios Tavernarakis, Shiliang Wang, Monica Driscoll Inhibition of touch cell fate in C. elegans Ji Wu, Anne Duggan, Martin Chalfie pag-3 and unc-3 interact to determine neural fates in the ventral cord, and their mammalian counterparts may similarly interact during blood cell development Scott Cameron, Brinda Prasad, Joan B. McDermott, Eric Aamodt, Randall R. Reed, Bob Horvitz Identification of sns-8, a serine/ threonine kinase, and additional genes required for the development of the AWB olfactory neurons. Anne Lanjuin, Piali Sengupta The bHLH transcription factors LIN-32 and HLH-2 specify multiple aspects of ray development in the C. elegans male tail Douglas S. Portman, Scott W. Emmons seu-1 Encodes Novel Nuclear Proteins Required for the Function of Ectopic UNC-5 D.C. Merz, H Zheng, A. Colavita, J.G. Culotti 8:48-9:10 p.m., BREAK 9:10-10:34 p.m., Session 2 Bill Kelly, Chair Molecular and genetic analyses of unc-69, a gene required for axon guidance Suzanne Tharin, Bruce Wightman, Nancy Tsung, Erika Hartwieg, Gian Garriga, Bob Horvitz, Michael Hengartner The C.
    [Show full text]
  • Green Fluorescent Protein ISSN 0306-0012 Guest Editors: Jeremy Sanders and Sophie Jackson TUTORIAL REVIEW HIGHLIGHT Wolf B
    Chemical Society Reviews www.rsc.org/chemsocrev Volume 38 | Number 10 | October 2009 | Pages 2813–2968 Themed issue: Green fluorescent protein ISSN 0306-0012 Guest editors: Jeremy Sanders and Sophie Jackson TUTORIAL REVIEW HIGHLIGHT Wolf B. Frommer, Michael W. Davidson Marc Zimmer and Robert E. Campbell GFP: from jelly"sh to the Nobel prize Genetically encoded biosensors based and beyond on engineered !uorescent proteins 0306-0012(2009)38:10;1-Q This article was published as part of the 2009 Green Fluorescent Protein issue Reviewing the latest developments in the science of green fluorescent protein Guest Editors Dr Sophie Jackson and Professor Jeremy Sanders All authors contributed to this issue in honour of the 2008 Nobel Prize winners in Chemistry, Professors Osamu Shimomura, Martin Chalfie and Roger Y. Tsien Please take a look at the issue 10 table of contents to access the other reviews HIGHLIGHT www.rsc.org/csr | Chemical Society Reviews GFP: from jellyfish to the Nobel prize and beyondw Marc Zimmer DOI: 10.1039/b904023d On December 10, 2008 Osamu Shimomura, Martin Chalfie and Roger Tsien were awarded the Nobel Prize in Chemistry for ‘‘the discovery and development of the green fluorescent protein, GFP’’. The path taken by this jellyfish protein to become one of the most useful tools in modern science and medicine is described. Osamu Shimomura painstakingly isolated GFP from hundreds of thousands of jellyfish, characterized the chromophore and elucidated the mechanism of Aequorean bioluminescence. Martin Chalfie expressed the protein in E. coli and C. elegans, and Roger Tsien developed a palette of fluorescent proteins that could be used in a myriad of applications.
    [Show full text]
  • 142 POSTERS: Cell Division and Growth Control
    142 POSTERS: Cell Division and Growth Control 157A An expression-based approach to identifying factors that mediate cell competition. Claire de la Cova, Laura A. Johnston. Genetics and Development, Columbia University, New York, NY. Cell competition is a process that results in elimination of “losing” cells and survival of “winning” cells within growing organs of both vertebrates and Drosophila melanogaster. In a mosaic Drosophila wing imaginal disc, cells expressing high levels of dMyc compete against and eliminate their neighbors with lower dMyc levels. “Losing” cells die frequently and their competitive elimination requires the pro-apoptotic gene hid. Because little is known about the signal that kills “losing” cells, and no biological markers specific to cell competition exist, we sought to better characterize “winning” and “losing” cells using a gene expression approach. We have generated and isolated competing cell populations from the wing imaginal disc and used gene expression microarrays identify mRNA expression changes in “winning” dMyc cells and wildtype “losing” cells. A large number of significant expression changes occur in dMyc-expressing cells (750 genes at ≥1.5-fold). On the other hand, when growing near dMyc-expressing cells, wildtype “losing” cells display a comparably small number of significant expression changes (58 genes at ≥1.5-fold). We have selected several candidate genes whose expression levels are altered in “losing” cells and used a simple clonal assay of cell competition in the wing imaginal disc to explore their roles in the competitive process. We find that some of our candidate genes contribute to the cell death or to the growth disadvantage of “losing” cells, while others are required for the survival of dMyc- expressing “winning” cells.
    [Show full text]
  • 2017-2018 General Studies Academic Policies
    The Major ........................................................ 37 TABLE OF Declaring a Major or Concentration ............ 38 Electives ............................................................ 41 CONTENTS Transfer Credit ................................................. 42 Under Construction .................................... 44 General Studies ..................................................................... 4 Majors and Concentrations ..................................... 44 GS Undergraduates ......................................................... 4 Declaring a Major ............................................. 38 Evening Courses ....................................................... 4 African-American Studies .................................. 48 Key to Course Listings ........................................ 4 American Studies .............................................. 49 Newly Approved Courses .............................. 5 Ancient Studies ................................................. 51 Summer Courses ......................................... 11 Anthropology .................................................... 52 History and Philosophy of Science .............. 11 Applied Mathematics ........................................ 55 Colloquia, Interdepartmental Seminars, and Professional School Offerings ...................... 12 Archaeology ...................................................... 60 Degree Fulfillment .................................................. 12 Architecture .....................................................
    [Show full text]
  • Enhancer Interactions in Developmental Gene Regulation
    Enhancer Interactions in Developmental Gene Regulation The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Biette, Kelly Marie. 2019. Enhancer Interactions in Developmental Gene Regulation. Doctoral dissertation, Harvard University, Graduate School of Arts & Sciences. Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:42029813 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use#LAA Enhancer Interactions in Developmental Gene Regulation A dissertation presented by Kelly Marie Biette to The Division of Medical Sciences in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the subject of Biological and Biomedical Sciences Harvard University Cambridge, Massachusetts May 2019! © 2019 Kelly Marie Biette All rights reserved.! Dissertation Advisor: Dr. Angela DePace Kelly Marie Biette Enhancer Interactions in Developmental Gene Regulation Abstract When and where a gene is expressed during development is a critical determinant of cell identity and transcriptional mis-regulation is a common driver of diverse disease states. The spatial and temporal expression of animal genes is controlled by enhancers, sequences of DNA that are bound by transcription factors (TFs) and direct the pattern, timing, and level of gene expression. Many developmental genes are surrounded by multiple enhancers, each of which directs a subset of the overall gene expression pattern, allowing the gene to be turned on at different stages or in different tissues throughout the lifetime of the organism.
    [Show full text]
  • Profile of Martin Chalfie
    PROFILE Profile of Martin Chalfie espite having a bad reputa- tion, cholesterol is an essen- tial component of the plasma membranes of animal cells, Dwhere it is thought to modulate the properties of the lipid bilayer. Choles- terol can also bind directly to proteins in the membrane. In his Inaugural Article published in 2006, Martin Chalfie, the William R. Kenan, Jr., Professor of Bio- logical Sciences at Columbia University (New York), in collaboration with Thomas Benzing (University of Cologne, Germany), identified a new class of cholesterol-binding proteins among the prohibitin (PHB)-domain protein family (1). PHB-domain proteins appear to regulate a variety of membrane func- tions, from cell signaling to mech- anosensation. Studying two members of the family, MEC-2 and Podocin, Chalfie, Benzing, and their colleagues found that cholesterol is crucial for the activity of two different classes of channel proteins to which the PHB-domain proteins bind. They suggest that the binding of choles- terol by the PHB-proteins alters the lo- cal lipid environment of associated membrane proteins and changes their activity. Questioning Research Chalfie, born in 1947 and elected to the National Academy of Sciences in 2004, grew up in Chicago. As a child, all ele- Martin Chalfie ments of science interested him, but he feels his early activities were somewhat Hastings provided Chalfie with one of Chalfie took a series of short-term jobs, mundane. ‘‘Unfortunately, I did not have including a stint selling dresses for his that real indicator of a career in science his fondest memories of Harvard. ‘‘I parents’ dress manufacturing business in that many of my friends have.
    [Show full text]
  • Andrew Martens 04/07/08 the Use of Terminators and Anti
    Andrew Martens 04/07/08 The Use of Terminators and Anti-Terminators to Expand the Two-Spatula Burnt-Pancake Problem in Escherichia coli Honors Thesis Davidson College First Reader: Dr. Campbell Second Reader: Dr. Wessner Abstract The emerging field of synthetic biology provides a new experimental approach to biological research by allowing for the design and implementation of novel genetic systems. In 2006, a joint effort by researchers from Davidson College and Missouri Western State University developed a bacterial system that uses the Salmonella Hin/hix DNA recombination mechanism to solve in vivo the two-spatula, burnt-pancake mathematical problem. The problem asks to calculate the least number of flips required to sort a given stack of pancakes with two spatulas, from largest to smallest and with all golden sides facing up, assuming that each pancake is burnt on one side and golden on the other. The system worked as a proof-of-concept for using bacteria to solve mathematical problems in vivo, but was limited to pancake stacks with 3 pancakes. A new system that uses transcription anti-terminators could potentially extend the size of the problem, allowing for many more pancakes per stack. I developed a way to use the naturally occurring E. coli trp operon anti-terminator, downstream of the pLac promoter, to control GFP gene expression in tryptophan-rich and tryptophan-poor conditions. RNA tertiary structure computer simulations were used to predict if the anti-terminator sequence could be modified to accommodate Hin-mediated flipping. The success of using an anti-terminator could enable synthetic biologists to design hundreds of anti-terminators that would allow bacteria to solve burnt-pancake problems that were exponentially more difficult.
    [Show full text]