Anti-Ruvc [Ruvc 5G9/3] Standard Size Ab01392-3.0

Anti-RuvC [RuvC 5G9/3] Standard Size, 200 μg, Ab01392-3.0 View online

Anti-RuvC [RuvC 5G9/3] Standard Size Ab01392-3.0

Developed in partnership with Ximbio (www.ximbio.com).

Isotype and Format: Mouse IgG2b, Kappa Clone Number: RuvC 5G9/3 Alternative Name(s) of Target: Crossover junction endodeoxyribonuclease RuvC; Holliday junction nuclease RuvC; Holliday junction resolvase RuvC; EC 3.1.22.4; 5G9 UniProt Accession Number of Target Protein: P0A814 Published Application(s): IP, WB Published Species Reactivity: E. coli Immunogen: This antibody was raised against RuvC protein from E. coli. Specificity: This antibody recognises E. coli RuvC. Application Notes: RuvC 5G9/3 (5G9) antibody was initially used to inhibit RuvC from interacting in a complex with other proteins and therefore to demonstrate the importance of this complex' formation in the resolution of Holliday junctions (Eggleston et al., 1997). IP: This antibody was also used to pull down complexes containing RuvC to analyse the interactions of RuvC with RuvA and RuvB (Davies and West, 1998). Antibody First Published in: Eggleston et al. In vitro reconstitution of the late steps of genetic recombination in E. coli. Cell. 1997 May 16;89(4):607-17. PMID:9160752 Note on publication: This article describes experimets for which RuvC 5G9/3 (5G9) antibody was created. The paper reports application of this anti-RuvC antibody to potentially inhibit creation of the RuvABC complex showing importance of the interactions of RuvC with other proteins in resolution of Holliday complexes.

Product Form

Size: 200 μg Purified antibody. Purification: Protein A affinity purified Supplied In: PBS with 0.02% Proclin 300. Storage Recommendation: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. Concentration: 1 mg/ml.

© 2021 Absolute Antibody https://absoluteantibody.com/product/anti-ruvc-ruvc-5g9-3/Ab01392- Wilton, UK. 3.0_mouse_igg2b/standard Important note – This product is for research use only. It is not intended for use in therapeutic or diagnostic procedures for humans or animals.