Catalog # Aliquot Size

W297-911-05 3 x 5 nmol W297-911-20 3 x 20 nmol W297-911-50 3 x 50 nmol

WWP2 siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # W297-911

Lot # Z2109-60

Specificity Formulation WWP2 siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human WWP2 expression. shipped at room temperature.

Product Description Reconstitution Protocol WWP2 siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human WWP2 mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_007014. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot AIP2; WWp2-like 1x stock solutions for storage.

Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or WWP1 Protein W296-30G below -20oC. After resuspension, siRNA stock solutions ≥2 WWP2 Protein W297-30G µM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background WWP2 or WW domain containing E3 ubiquitin protein WWP2 siRNA Set I 2 is a member of the Nedd4 family of E3 , siRNA duplexes targeted against three exon regions which play an important role in protein ubiquitination. WWP2 contains four WW domains and may play a role in Catalog # W297-911 multiple processes including chondrogenesis and the Lot # Z2109-60 regulation of oncogenic signaling pathways via Packaging Specifications 2.5 nmol/tube for 3 x 5nmol interactions with Smad proteins and the tumor suppressor Format Lyophilized powder PTEN. WWP2 is an E3 that targets Stability 1yr at -70oC from date of shipment transcription factor Oct-4 for ubiquitination (1). WWP2 Storage & Shipping The lyophilized powder is stable for at which is also called AIP2 can bind to atrophin-1 (2). least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at References or below -20oC. After resuspension, 1. Xu, H. M. et.al: Wwp2, an E3 ubiquitin ligase that siRNA stock solutions ≥2 µ M can undergo up to 50 freeze-thaw cycles targets transcription factor Oct-4 for ubiquitination. J. without significant degradation. For Biol. Chem. 279: 23495-23503, 2004. long-term storage, it is recommended 2. Wood, J. D.et.al: Atrophin-1, the DRPLA gene that the siRNA is stored at -70oC. For most favorable performance, avoid product, interacts with two families of WW domain- repeated Handling and multiple containing proteins. Molec. Cell. Neurosci. 11: 149- freeze/thaw cycles. 160, 1998.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.