Catalog # Aliquot Size W297-911-05 3 x 5 nmol W297-911-20 3 x 20 nmol W297-911-50 3 x 50 nmol WWP2 siRNA Set I siRNA duplexes targeted against three exon regions Catalog # W297-911 Lot # Z2109-60 Specificity Formulation WWP2 siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human WWP2 expression. shipped at room temperature. Product Description Reconstitution Protocol WWP2 siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human WWP2 mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_007014. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot AIP2; WWp2-like 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or WWP1 Protein W296-30G below -20oC. After resuspension, siRNA stock solutions ≥2 WWP2 Protein W297-30G µM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Scientific Background WWP2 or WW domain containing E3 ubiquitin protein WWP2 siRNA Set I ligase 2 is a member of the Nedd4 family of E3 ligases, siRNA duplexes targeted against three exon regions which play an important role in protein ubiquitination. WWP2 contains four WW domains and may play a role in Catalog # W297-911 multiple processes including chondrogenesis and the Lot # Z2109-60 regulation of oncogenic signaling pathways via Packaging Specifications 2.5 nmol/tube for 3 x 5nmol interactions with Smad proteins and the tumor suppressor Format Lyophilized powder PTEN. WWP2 is an E3 ubiquitin ligase that targets Stability 1yr at -70oC from date of shipment transcription factor Oct-4 for ubiquitination (1). WWP2 Storage & Shipping The lyophilized powder is stable for at which is also called AIP2 can bind to atrophin-1 (2). least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at References or below -20oC. After resuspension, 1. Xu, H. M. et.al: Wwp2, an E3 ubiquitin ligase that siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles targets transcription factor Oct-4 for ubiquitination. J. without significant degradation. For Biol. Chem. 279: 23495-23503, 2004. long-term storage, it is recommended 2. Wood, J. D.et.al: Atrophin-1, the DRPLA gene that the siRNA is stored at -70oC. For most favorable performance, avoid product, interacts with two families of WW domain- repeated Handling and multiple containing proteins. Molec. Cell. Neurosci. 11: 149- freeze/thaw cycles. 160, 1998. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .