Chlorella Vulgaris Biofilter Chlorella Vulgaris F/2 Scenedesmus Sp
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A CONTINUOUS RECIRCULATION PHOTOBIOREACTOR FOR INSERT BIODESALINATION OF SALINE WATER USING HALOPHYTIC ALGAE LOGO HERE Ashraf Aly Hassan, Ph.D., P.E.a&b and Endalkachew Sahle-Demessie, Ph.D. c a Department of Civil Engineering, University of Nebraska-Lincoln b Department of Civil and Environmental Engineering, United Arab Emirates University c US Environmental Protection Agency, Office of Research & Development Biodesalination - A Concept Batch Reactor Performance Sea Salt (Instant Ocean) NaCl Euglena deses 20 g/L Oscillatoria tenuis 25 g/L Removal of different bottles by Euglena deses @ 20 g/L Removal of different bottles by Oscillatoria tenuis @ 25 g/L Scenedesmus Sp. Biofilter Scenedesmus Sp. F/2 2 g/L 1.00 1.00 30 30 Chlorella Vulgaris Biofilter Chlorella Vulgaris F/2 Scenedesmus Sp. F/2 0.95 0.98 Chlorella Vulgaris Carolina Chlorella Vulgaris Biofilter Chlorella Vulgaris F/2 0.90 Chlorella Vulgaris Carolina 0.96 20 20 0 0 0.85 C/C C/C 0.94 0.80 % Removal % Removal, % Removal, Separation Salinity 0.92 10 10 0.75 Saline Reduced water water 0.70 0.90 0 20 40 60 80 0 20 40 60 80 Algal biomass Sequential time, days Sequential time, days 0 0 CO2 10 20 30 40 50 10 20 30 40 50 Protein, Consecutive time, days Consecutive time, days Carbohydrate, - Halophyte algae Energy 4 g/L Sample Cell Conc., Cl conc., Decrease Photobioreactor 30 30 Scenedesmus Sp. Biofilter - Scenedesmus Sp. F/2 in Cl , % Chlorella Vulgaris Biofilter Cell/ml mV Chlorella Vulgaris F/2 4 ❑The concept of bio-desalination is novel, Chlorella Vulgaris Carolina Full bloom ~10 12.4 21 and research is still in its infancy 20 20 Centrifuge ~102 7.2 58 % Removal % Removal, % Removal, ❑Macrophyte algae sequester salts in and 10 10 Scenedesmus Sp. Biofilter Scenedesmus Sp. F/2 Centrifuged Negligible 3.2 74 remove them from solution Chlorella Vulgaris Biofilter Chlorella Vulgaris F/2 and filtered Chlorella Vulgaris Carolina 0 0 ❑Salt uptake occurs due to accumulation 10 20 30 40 50 10 20 30 40 50 Consecutive time, days Consecutive time, days against an increased osmotic gradient 1000 Type of Algae Phylum Halophyte Desalination Scenedsmus sp. Scenedesmus Sp. Chlorophyta Marine/ Fresh 30% 100 Chlorella vulgaris Chlorella vulgaris Chlorophyta Fresh 25% 10 Oscillatoria tenuis Cyanobacteria Marine/ Fresh 20% Euglena deses Euglenozoa Marine 15% 1 Total ion mass, mg Cladophora delicatula Chlorophyta Marine None 0.1 Dunaliella salina Chlorophyta Marine None Compsopogon 0.01 Rhodophyta Marine/ Fresh None Al Ba Ca Co Cu Fe K Mg Mn Mo Na Ni P Pb S Sb Se Sr Zn coeruleus Ion Chlorella autotrophica Chlorophyta Marine Minimal Spirulina maxima Cyanobacteria Fresh Minimal Spirulina platensis Cyanobacteria Marine/ Fresh Minimal Upscaling 1.00 0 0.95 5 Species tested 0.90 10 Light 0 Filter 0.85 15 C/C Algae PBR recovery Scenedesmus sp. Chlorella vulgaris 0.80 Scenedesmus sp., F/2 media, 4 g/L 20 % Removal, Scenedesmus sp., Biofilter media, 4 g/L •Fresh and marine species •Used frequently as a model Air+CO2 0.75 Scenedesmus sp., Biofilter media, 20 g/L 25 •Resulted in 25% salt reduction algae Chlorella vulgaris, F/2 media, 4 g/L Pump Chlorella vulgaris, Biofilter media, 4 g/L of seawater (ElSayed 2010) •Despite the fact that Chlorella 0.70 30 •Available in most waterways vulgaris is often found in 0 20 40 60 80 and oceans freshwater, it is shown that it Consecutive time, days •High in oil content could resist salinity up to 20g/LA •Original publication 1829 NaCl B •Original publication: 1890 •Chlorella contains 10% minerals (w/w, dry basis) among other valuable compounds Acknowledgement & Disclaimer Oscillatoria tenuis Euglena deses var. ❑ Scan the QR Code for more details in a vermiformis published article • Cyanobacteria filamentous •Original Publication: Euglena ❑ Please. DO NOT TAKE PICTURES of this blue-green algae deses Ehrenberg 1833 poster. It includes proprietary data. •Motile by shooting •This is a marine species. LDE Light ❑ The findings and conclusions of this poster polysaccharides •Isolation: E.G. Pringsheim lamp Filter •Original publication: Agardh, (1951) have not been formally disseminated by U.S. C.A. (1813). Algarum decas •Behaves like duckweeds Photo-bio EPA and should not be understood to represent secunda. pp. 17-28. Lundae (Flooting) Algae reactor recovery any agency determination or policy. The views [Lund]: Litteris Berlingianis. Air+CO2 expressed in this presentation are those of the authors and do not necessarily represent the AirCO2 Mix er views or policies of the U.S. EPA ❑ Funding through the EPA "Pathfinder Innovation Projects" challenge in pursuit of Pump high-risk, high-reward research ideas Mass flow Controllers www.meteconferences.org.