Estrone Sulfate
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Testosterone, DHP, Progesterone, Es
UC Davis Clinical Endocrinology Laboratory Volume Required: Testosterone: 2 mL serum AMH equine: 1 ml serum Estrone Sulfate, Progesterone: 1 mL serum each. Inhibin: 1 ml serum, sent overnight on ice. AMH Canine/Feline Spaychek: 200 µL serum, fasted, 30 days post- surgery. Send 0.5 ml for Progesterone/AMH and 2 ml for testosterone/AMH. Cryptorchid Panel: 2 mL serum Pregnancy Panel: 2 mL serum Granulosa Cell Tumor Panel: 3 mL serum Sample Handling and Shipment Requirements: PLEASE SEND SERUM ONLY, no whole or clotted blood. Blood contains active enzymes which may affect the results. The use of serum separator tubes is not recommended; they may degrade the analytes, particularly progesterone and AMH, and may invalidate results. Draw in a tube with no additive (red top). If you do use a serum separator tube, transfer the serum to a new tube as soon as possible. For AMH and inhibin testing: Please separate the serum and ship priority overnight on an ice pack. Store the sample in the freezer if shipping will be delayed, but you may ship it on an ice pack, dry ice is not required. Do not ship the sample via the US Postal Service, as the delivery will be delayed in the campus mailroom for up to a week, causing sample degradation. Do not ship the samples to arrive on a holiday or a weekend, as UPS and Fed Ex will not deliver it to us, and it will sit at the shipping facility, causing sample degradation. Please check our site for university holidays. For steroid hormone (testosterone, DHP, progesterone, estrone) testing: These hormones are more stable; however, they may be degraded by poor handling conditions, and shipment as whole or clotted blood. -
Alteration of the Steroidogenesis in Boys with Autism Spectrum Disorders
Janšáková et al. Translational Psychiatry (2020) 10:340 https://doi.org/10.1038/s41398-020-01017-8 Translational Psychiatry ARTICLE Open Access Alteration of the steroidogenesis in boys with autism spectrum disorders Katarína Janšáková 1, Martin Hill 2,DianaČelárová1,HanaCelušáková1,GabrielaRepiská1,MarieBičíková2, Ludmila Máčová2 and Daniela Ostatníková1 Abstract The etiology of autism spectrum disorders (ASD) remains unknown, but associations between prenatal hormonal changes and ASD risk were found. The consequences of these changes on the steroidogenesis during a postnatal development are not yet well known. The aim of this study was to analyze the steroid metabolic pathway in prepubertal ASD and neurotypical boys. Plasma samples were collected from 62 prepubertal ASD boys and 24 age and sex-matched controls (CTRL). Eighty-two biomarkers of steroidogenesis were detected using gas-chromatography tandem-mass spectrometry. We observed changes across the whole alternative backdoor pathway of androgens synthesis toward lower level in ASD group. Our data indicate suppressed production of pregnenolone sulfate at augmented activities of CYP17A1 and SULT2A1 and reduced HSD3B2 activity in ASD group which is partly consistent with the results reported in older children, in whom the adrenal zona reticularis significantly influences the steroid levels. Furthermore, we detected the suppressed activity of CYP7B1 enzyme readily metabolizing the precursors of sex hormones on one hand but increased anti-glucocorticoid effect of 7α-hydroxy-DHEA via competition with cortisone for HSD11B1 on the other. The multivariate model found significant correlations between behavioral indices and circulating steroids. From dependent variables, the best correlation was found for the social interaction (28.5%). Observed changes give a space for their utilization as biomarkers while reveal the etiopathogenesis of ASD. -
The Relationship Between Serum Sex Steroid Levels and Heart Rate Variability Parameters in Males and the Effect Of
Türk Kardiyol Dern Arş - Arch Turk Soc Cardiol 2010;38(7):459-465 459 The relationship between serum sex steroid levels and heart rate variability parameters in males and the effect of age Erkeklerde serum cinsiyet steroidleri ile kalp hızı değişkenliği verileri arasındaki ilişki ve yaşın etkileri M. Tolga Doğru, M.D., M. Murad Başar, M.D.,# Ercan Yuvanç, M.D.,# Vedat Şimşek, M.D., Ömer Şahin, M.D. Departments of Cardiology and #Urology, Medicine Faculty of Kırıkkale University, Kırıkkale Objectives: We evaluated the relationships between sex Amaç: Bu çalışmada cinsiyet steroidleri ile kalp hızı de- steroid levels and heart rate variability (HRV) parameters. ğişkenliği (KHD) verileri arasındaki ilişkiler araştırıldı. Study design: The study included 114 male subjects (mean Çalışma planı: Çalışmaya, kardiyolojik açıdan değerlen- age 46.6±11.3 years) presenting to our department for cardi- dirme için başvuran 114 erkek hasta (ort. yaş 46.6±11.3) ologic evaluation. Hormonal analysis included serum levels alındı. Hormon analizlerinde serumda luteinize edici hor- of luteinizing hormone, prolactin, total testosterone (TT), free mon, prolaktin, total testosteron (TT), serbest testosteron, testosterone, estradiol (E2), and dehydroepiandrosterone östradiol (E2) ve dehidroepiandrosteron sülfat (DHEA-S) sulfate (DHEA-S). Parameters of HRV were derived from düzeyleri ölçüldü. Yirmi dört saatlik Holter kayıtlarından 24-hour Holter monitoring. The associations between serum KHD parametreleri hesaplandı. Serum cinsiyet steroidleri sex steroid levels and HRV parameters were investigated in ile KHD değerleri arasındaki ilişkiler hastalar üç yaş gru- three age groups (20-39 years; 40-59 years; >60 years). buna (20-39 yaş; 40-59 yaş; >60 yaş) ayrılarak incelendi. -
Conjugated and Unconjugated Plasma Androgens in Normal Children
Pediat. Res. 6: 111-118 (1972) Androstcncdionc sexual development dehydroepiandrosterone testosterone Conjugated and Unconjugated Plasma Androgens in Normal Children DONALD A. BOON,'291 RAYMOND E. KEENAN, W. ROY SLAUNWHITE, JR., AND THOMAS ACETO, JR. Children's Hospital, Medical Foundation of Buffalo, Roswell Park Memorial Institute, and Departments of Biochemistry and Pediatrics, School of Medicine, State University of New York at Buffalo, Buffalo, New York, USA Extract Methods developed in this laboratory permit measurement of the androgens, testos- terone (T), dehydroepiandrosterone (D), and androstenedione (A) on a 10-ml sample of plasma. We have determined concentrations of the unconjugated androgens (T, A, D) as well as of the sulfates of dehydroepiandrosterone (DS) and androsterone (AS) in the plasma of 85 healthy children of both sexes from birth through the age of 20 years. Our results are shown and summarized, along with those of other investigators. Mean plasma concentrations Sex and age ng/100 ml Mg/100 ml T A D AS DS Male Neonates 39 24 30 None detectable None detectable 1-8 years 25 58 64 2 11 9-20 years 231 138 237 28 74 Female Neonates 36 30 83 None detectable None detectable 1-8 years 11 69 54 5 20 9-20 years 28 84 164 22 44 Testosterone was elevated in both sexes in the newborn as compared with the 1-8- year-old group. In contrast, sulfated androgens, with one exception, were undetectable early in life. In males, there was a marked rise in all androgens, especially T, in the 9-18-year-old group. -
TX-004HR Vaginal Estradiol Has Negligible to Very Low Systemic Absorption of Estradiol
Himmelfarb Health Sciences Library, The George Washington University Health Sciences Research Commons Obstetrics and Gynecology Faculty Publications Obstetrics and Gynecology 12-19-2016 TX-004HR vaginal estradiol has negligible to very low systemic absorption of estradiol. David F Archer Ginger D Constantine James A Simon George Washington University Harvey Kushner Philip Mayer See next page for additional authors Follow this and additional works at: http://hsrc.himmelfarb.gwu.edu/smhs_obgyn_facpubs Part of the Female Urogenital Diseases and Pregnancy Complications Commons, Obstetrics and Gynecology Commons, and the Women's Health Commons APA Citation Archer, D., Constantine, G., Simon, J., Kushner, H., Mayer, P., Bernick, B., Graham, S., Mirkin, S., & REJOICE Study Group. (2016). TX-004HR vaginal estradiol has negligible to very low systemic absorption of estradiol.. Menopause (New York, N.Y.), 24 (5). http://dx.doi.org/10.1097/GME.0000000000000790 This Journal Article is brought to you for free and open access by the Obstetrics and Gynecology at Health Sciences Research Commons. It has been accepted for inclusion in Obstetrics and Gynecology Faculty Publications by an authorized administrator of Health Sciences Research Commons. For more information, please contact [email protected]. Authors David F Archer, Ginger D Constantine, James A Simon, Harvey Kushner, Philip Mayer, Brian Bernick, Shelli Graham, Sebastian Mirkin, and REJOICE Study Group. This journal article is available at Health Sciences Research Commons: http://hsrc.himmelfarb.gwu.edu/smhs_obgyn_facpubs/146 CE: M.S.; MENO-D-16-00223; Total nos of Pages: 7; MENO-D-16-00223 Menopause: The Journal of The North American Menopause Society Vol. 24, No. -
A New Aromatase Inhibitor, in Postmenopausal Women
(CANCERRESEARCH52, 5933-5939, November1, 1992J Phase I and Endocrine Study of Exemestane (FCE 24304), a New Aromatase Inhibitor, in Postmenopausal Women T. R. Jeffry Evans,' Enrico Di Salle, Giorgio Ornati, Mercedes Lassus, Margherita Strolin Benedetti, Eio Pianezzola, and R. Charles Coombes Department ofMedical OncoIoij@,St. Geoa@ge'sHospital Medical School, Creamer Terrace, London SWI7 ORE, England fT. R. I. E.J; Departments of Oncology IE. D. S., G. 0., M. Li and Pharmacokinetics and Metabolism [M. S. B., E. P.J, Farmitalia Carlo Erba, Via Carlo Imbonati, Milan, Italy; and Department of Medical Oncology, Charing Cross Hospital, FuThoin Palace Roa@ London W6 8RF, England (R. C. C.] ABSTRACT aminoglutethimide and fadrozole (CGS 16949A) (7). Objective tumor regression occurred in approximately 21% of patients Aromatase inhibitors are a useful therapeutic option in the manage treated with 4}IAI@@,2witha low incidence of adverse effects; ment of endocrine-dependent advanced breast cancer. A single-dose 4.5% of patients were withdrawn from treatment because of administration of exemestane (FCE 24304; 6-methylenandrosta-l,4-dl ene-3,17-dione), a new Irreversible aromatase inhibitor, was investi side effects. However, 4HAD undergoes extensive metabolism gated in 29 healthy postmenopausal female volunteers. The compound, in the liver to form the inactive glucuronide (8) and conse given at p.o. doses ofO.5, 5, 12.5, 25, 50, 200, 400, and 800 mg(n = 3—4), quently it is recommended that it is given i.m. rather than p.o. was found to be a well tolerated, potent, long-lasting, and specific in The use of aminoglutethimide as an aromatase inhibitor is re hibitor of estrogen biosynthesis. -
Memory-Enhancing Effects in Male Mice of Pregnenolone and Steroids Metabolically Derived from It
Proc. Nati. Acad. Sci. USA Vol. 89, pp. 1567-1571, March 1992 Neurobiology Memory-enhancing effects in male mice of pregnenolone and steroids metabolically derived from it (memory enhancement/pregnenolone sulfate/receptors/immediate-early genes/aging) JAMES F. FLOOD*t, JOHN E. MORLEY*, AND EUGENE ROBERTSt§ *Genatnc Research Education and Clinical Center, Veterans Administration Medical Center, St. Louis, MO 63106, and St. Louis University School of Medicine, Division of Geriatric Medicine, St. Louis, MO 63104; and tDepartment of Neurobiochemistry, Beckman Research Institute of the City of Hope, Duarte, CA 91010 Contributed by Eugene Roberts, November 8, 1991 ABSTRACT Immediate post-training intracerebroven- postmitotic differentiated states was favored in both neurons tricular administration to male mice of pregnenolone (P), and glia (7, 8). pregnenolone sulfate (PS), dehydroepiandrosterone (DHEA), The water-soluble DHEAS, administered intracerebro- dehydroepiandrosterone sulfate (DHEAS), androstenedione, ventricularly (i.c.v.) or subcutaneously (s.c.) after training, testosterone, dihydrotestosterone, or aldosterone caused im- showed convincing memory-enhancing (ME) effects in foot- provement ofretention for footshock active avoidance training, shock active avoidance training (FAAT) in undertrained male while estrone, estradiol, progesterone, or 16.8-bromoepi- mice. DHEAS administered i.c.v. facilitated retention for androsterone did not. Dose-response curves were obtained for step-down passive avoidance training. Retention of FAAT P, PS, DHEA, and testosterone. P and PS were the most potent, was improved when DHEAS was given in taste-camouflaged PS showing significant effects at 3.5 fmol per mouse. The active drinking water for 1 week before and 1 week after training, steroids did not show discernible structural features or known while DHEAS in the drinking water for 2 weeks did not membrane or biochemical effects that correlated with their improve acquisition (9). -
Steroid Secretion. Newly Discovered Functions in the Brain
Steroid secretion. Newly Discovered Functions in the Brain. Fundamental and Clinical considerations. Bernardo O. Dubrovsky McGill University Medical School Montreal, Quebec, Canada B.O. Dubrovsky, 3445 Drummond Street, #701, Montreal, Quebec, H3G 1X9,Canada. Tel: (514) 844-5702 Fax: (514) 398-4370 E-mail: [email protected] Abstract While the relationships between endocrines and psychiatry have long been established, the implications of neurosteroid (NS) hormones, identified in the early 1980s for psychopathology, started to be recognized in the nineties. Tetrahydro metabolites of progesterone (ATHP) and deoxycorticosterone (ATHDOC) act as positive allosteric modulators of neurotransmitter receptors such as GABAA. Other NSs, i.e., androsterone, pregnenolone, dehydroepiandrosterone (DHEA), their sulfates and lipid derivatives modulate glycine-activated chloride channels, neural nicotinic acetylcholine receptors constituted in Xenopus laevis oocytes, and voltage-activated calcium channels. Sigma receptors, as pharmacologically defined by their effect on N-methyl-D- aspartate (NMDA) activity, have been studied in rat hippocampal preparations: here DHEAS acts as a sigma receptor agonist, differently from pregnenolone which appears as a sigma inverse agonist and progesterone which behaves as an antagonist. All of them were identified as NSs. Neuroactive steroids rapidly change CNS excitability and produce behavioral effects within seconds to minutes following administration to both laboratory animals and man. These fast actions probably indicate membrane mediated effects. This notwithstanding, Rupprecht et al. showed that after oxidation, ring A reduced pregnanes can regulate gene expression via the progesterone intracellular receptor. The necessary enzymes for the metabolism of primary adrenal and gonadal steroids, a peripheral source for NSs, exist in the brain in a compartmentalized way. -
Memory-Enhancing Effects in Male Mice of Pregnenolone and Steroids
Proc. Nati. Acad. Sci. USA Vol. 89, pp. 1567-1571, March 1992 Neurobiology Memory-enhancing effects in male mice of pregnenolone and steroids metabolically derived from it (memory enhancement/pregnenolone sulfate/receptors/immediate-early genes/aging) JAMES F. FLOOD*t, JOHN E. MORLEY*, AND EUGENE ROBERTSt§ *Genatnc Research Education and Clinical Center, Veterans Administration Medical Center, St. Louis, MO 63106, and St. Louis University School of Medicine, Division of Geriatric Medicine, St. Louis, MO 63104; and tDepartment of Neurobiochemistry, Beckman Research Institute of the City of Hope, Duarte, CA 91010 Contributed by Eugene Roberts, November 8, 1991 ABSTRACT Immediate post-training intracerebroven- postmitotic differentiated states was favored in both neurons tricular administration to male mice of pregnenolone (P), and glia (7, 8). pregnenolone sulfate (PS), dehydroepiandrosterone (DHEA), The water-soluble DHEAS, administered intracerebro- dehydroepiandrosterone sulfate (DHEAS), androstenedione, ventricularly (i.c.v.) or subcutaneously (s.c.) after training, testosterone, dihydrotestosterone, or aldosterone caused im- showed convincing memory-enhancing (ME) effects in foot- provement ofretention for footshock active avoidance training, shock active avoidance training (FAAT) in undertrained male while estrone, estradiol, progesterone, or 16.8-bromoepi- mice. DHEAS administered i.c.v. facilitated retention for androsterone did not. Dose-response curves were obtained for step-down passive avoidance training. Retention of FAAT P, PS, DHEA, and testosterone. P and PS were the most potent, was improved when DHEAS was given in taste-camouflaged PS showing significant effects at 3.5 fmol per mouse. The active drinking water for 1 week before and 1 week after training, steroids did not show discernible structural features or known while DHEAS in the drinking water for 2 weeks did not membrane or biochemical effects that correlated with their improve acquisition (9). -
A Comprehensive Analysis of Steroid Hormones and Progression of Localized High-Risk Prostate Cancer
Published OnlineFirst February 7, 2019; DOI: 10.1158/1055-9965.EPI-18-1002 Research Article Cancer Epidemiology, Biomarkers A Comprehensive Analysis of Steroid Hormones & Prevention and Progression of Localized High-Risk Prostate Cancer Eric Levesque 1, Patrick Caron2, Louis Lacombe1,Veronique Turcotte2, David Simonyan3, Yves Fradet1, Armen Aprikian4, Fred Saad5, Michel Carmel6, Simone Chevalier4, and Chantal Guillemette2 Abstract Background: In men with localized prostate cancer who are terone levels were higher in low-risk disease. Associations were undergoing radical prostatectomy (RP), it is uncertain whether observed between adrenal precursors and risk of cancer pro- their systemic hormonal environment is associated with out- gression. In high-risk patients, a one-unit increment in log- comes. The objective of the study was to examine the associ- transformed androstenediol (A5diol) and dehydroepiandros- ation between the circulating steroid metabolome with prog- terone-sulfate (DHEA-S) levels were linked to DFS with HR of nostic factors and progression. 1.47 (P ¼ 0.0017; q ¼ 0.026) and 1.24 (P ¼ 0.043; q ¼ 0.323), Methods: The prospective PROCURE cohort was recruited respectively. Although the number of metastatic events was from 2007 to 2012, and comprises 1,766 patients with local- limited, trends with metastasis-free survival were observed for ized prostate cancer who provided blood samples prior to RP. A5diol (HR ¼ 1.51; P ¼ 0.057) and DHEA-S levels (HR ¼ 1.43; The levels of 15 steroids were measured in plasma using mass P ¼ 0.054). spectrometry, and their association with prognostic factors and Conclusions: In men with localized prostate cancer, our disease-free survival (DFS) was established with logistic regres- data suggest that the preoperative steroid metabolome is sion and multivariable Cox proportional hazard models. -
Other Data Relevant to an Evaluation of Carcinogenicity and Its Mechanisms
COMBINED ESTROGEN−PROTESTOGEN MENOPAUSAL THERAPY 263 4. Other Data Relevant to an Evaluation of Carcinogenicity and its Mechanisms 4.1 Absorption, distribution, metabolism and excretion The distribution of progestogens is described in the monograph on Combined estro- gen–progestogen contraceptives. That of estrogens is described below. 4.1.1 Humans Little more has been discovered about the absorption and distribution of estrone, estradiol and estriol products and conjugated equine estrogens in humans since the previous evaluation (IARC, 1999). Greater progress has been made in the identification and characterization of the enzymes that are involved in estrogen metabolism and excre- tion. The various metabolites and the responsible enzymes, including genotypic varia- tions, are described below (see Figures 3 and 4). Sulfation and glucuronidation are the main metabolic reactions of estrogens in humans. (a) Metabolites (i) Estrogen sulfates Several members of the sulfotransferase (SULT) gene family can sulfate hydroxy- steroids, including estrogens. The importance of SULTs in estrogen conjugation is demons- trated by the observation that a major component of circulating estrogen is sulfated, i.e. estrone sulfate (reviewed by Pasqualini, 2004). In addition to the parent hormones, estrone and estradiol, SULTs can also conjugate their respective catechols and also methoxyestro- gens (Spink et al., 2000; Adjei & Weinshilboum, 2002). The resulting sulfated metabolites are more hydrophilic and can be excreted. In postmenopausal breast cancers, levels of estrone sulfate can reach 3.3 ± 1.9 pmol/g tissue, which is five to nine times higher than the corresponding plasma concentration (equating gram of tissue with millilitre of plasma) (Pasqualini et al., 1996). In contrast, levels of estrone sulfate in premenopausal breast tumours are two to four times lower than those in plasma. -
Hormonal Health
Hormonal Health Patient: SAMPLE PATIENT Age: Sex: MRN: Reference Range Reference Range Hormone Reference Range Hormone Reference Range Progesterone 0.61 0.30-1.13 ng/mL DHEA-S 91 35-430 mcg/dL Testosterone 0.34 0.10-0.80 ng/mL Free Androgen 1.47 0.22-5.78 Index Reference Range Hormone Reference Range Sex Hormone 80 18-114 nmol/L Binding Globulin Reference Range Hormone Reference Range 2-Hydroxyestrone 299 112-656 pg/mL Reference Range Hormone Reference Range 16α-Hydroxyestrone 323 213-680 pg/mL Estrone 2.18 0.56-2.67 ng/mL Sulfate (E1S) 2:16α-Hydroxy- 0.93 0.40-1.40 Estrone Ratio Estrone (E1) 85 20-95 pg/mL Estradiol (E2) 33 20-160 pg/mL Estriol (E3) 113 <= 80 pg/mL Reference ranges for the estrogen metabolites were determined with serum samples from women with a normal 2:16alpha-Hydroxyestrone Ratio. Other reference ranges were determined with follicular serum samples from premenopausal women who were not using hormone replacement therapy. These ranges serve as clinical guidelines to observe changes due to hormone replacement. However, each individual is unique and treatment should be tailored to the patient's clinical picture. © Genova Diagnostics · CLIA Lic. #34D0655571 · Medicare Lic. #34-8475 GDX-4-192 Patient: SAMPLE PATIENT ID: Page 2 0.61 91 0.34 80 2.18 33 85 113 299 323 © Genova Diagnostics · CLIA Lic. #34D0655571 · Medicare Lic. #34-8475 Patient: SAMPLE PATIENT ID: Page 3 Premenopausal Premenopausal Unsupplemented Analyte follicular luteal Menopausal Patient Results Estrone Sulfate 0.56 - 2.67 0.75 - 4.28 0.23 - 1.40 2.18 (ng/mL) Estrone 20 - 95 28 - 163 12 - 41 85 (pg/mL) Estradiol 20 - 160 27 - 246 20 - 24 33 (pg/mL) Estriol <= 80 <= 80 <= 80 113 (pg/mL) Progesterone 0.30 - 1.13 0.95 - 21.00 0.30 - 0.64 0.61 (ng/mL) Testosterone 0.10 - 0.80 0.10 - 0.80 0.07 - 0.46 0.34 (ng/mL) DHEA-s 35 - 430 35 - 430 30 - 202 91 (mcg/dL) © Genova Diagnostics · CLIA Lic.