DOCSLIB.ORG

Publications for Patrick Tam 2020 2019 2018

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Publications for Patrick Tam 2020 2019 2018 Publications for Patrick Tam 2021 Kim, H., Tam, P., Yang, P. (2021). Defining cell identity 2019 beyond the premise of differential gene expression. Cell Regeneration, 10(1), 20-3 pages. <a Osteil, P., Studdert, J., Goh, H., Wilkie, E., Fan, X., Khoo, P., href="http://dx.doi.org/10.1186/s13619-021-00083-7">[More Peng, G., Salehin, N., Knowles, H., Han, J., Fossat, N., Tam, P., Information]</a> et al (2019). Dynamics of Wnt activity on the acquisition of ectoderm potency in epiblast stem cells. Development, 146(7), Rossant, J., Tam, P. (2021). Opportunities and challenges with 1-14. <a href="http://dx.doi.org/10.1242/dev.172858">[More stem cell-based embryo models. Stem Cell Reports, 16(5), 1031- Information]</a> 1038. <a href="http://dx.doi.org/10.1016/j.stemcr.2021.02.002">[More Gao, X., Nowak-Imialek, M., Chen, X., Chen, D., Herrmann, Information]</a> D., Ruan, D., Chen, A., Eckersley-Maslin, M., Ahmad, S., Lee, Y., Tam, P., et al (2019). Establishment of porcine and human Fan, X., Masamsetti, V., Sun, J., Engholm-Keller, K., Osteil, P., expanded potential stem cells. Nature Cell Biology, 21(6), 687- Studdert, J., Graham, M., Fossat, N., Tam, P. (2021). TWIST1 699. <a href="http://dx.doi.org/10.1038/s41556-019-0333- and chromatin regulatory proteins interact to guide neural crest 2">[More Information]</a> cell differentiation. eLife, 10, e62873. <a href="http://dx.doi.org/10.7554/eLife.62873">[More Sibbritt, T., Osteil, P., Fan, X., Sun, J., Salehin, N., Knowles, Information]</a> H., Shen, J., Tam, P. (2019). Gene Editing of Mouse Embryonic and Epiblast Stem Cells. In Ivan Bertoncello (Eds.), Mouse 2020 Cell Culture: Methods and Protocols, (pp. 77-95). New York: Springer. <a href="http://dx.doi.org/10.1007/978-1-4939-9086- Peng, G., Suo, S., Cui, G., Yu, F., Wang, R., Chen, J., Chen, S., 3_6">[More Information]</a> Liu, Z., Chen, G., Qian, Y., Tam, P., et al (2020). Author Correction: Molecular architecture of lineage allocation and McMahon, R., Sibbritt, T., Salehin, N., Osteil, P., Tam, P. tissue organization in early mouse embryo. Nature, 577(7791), (2019). Mechanistic insights from the LHX1-driven molecular E6. <a href="http://dx.doi.org/10.1038/s41586-019-1887- network in building the embryonic head. Development, Growth 7">[More Information]</a> and Differentiation, 61(5), 327-336. <a href="http://dx.doi.org/10.1111/dgd.12609">[More Tam, P., Ho, J. (2020). Cellular diversity and lineage trajectory: Information]</a> insights from mouse single cell transcriptomes. Development, 147(2), dev179788. <a Tam, P. (2019). Modeling the early development of a primate href="http://dx.doi.org/10.1242/dev.179788">[More embryo. Science, 366(6467), 798-799. <a Information]</a> href="http://dx.doi.org/10.1126/science.aaz6976">[More Information]</a> Shim, W., Sinniah, E., Xu, J., Vitrinel, B., Alexanian, M., Andreoletti, G., Shen, S., Sun, Y., Balderson, B., Boix, C., Peng, G., Suo, S., Cui, G., Yu, F., Wang, R., Chen, J., Chen, S., Tam, P., et al (2020). Conserved Epigenetic Regulatory Logic Liu, Z., Chen, G., Qian, Y., Tam, P., et al (2019). Molecular Infers Genes Governing Cell Identity. Cell Systems, 11(6), 625- architecture of lineage allocation and tissue organization in 639.e13. <a early mouse embryo. Nature, 572, 528-532. <a href="http://dx.doi.org/10.1016/j.cels.2020.11.001">[More href="http://dx.doi.org/10.1038/s41586-019-1469-8">[More Information]</a> Information]</a> Hamada, H., Tam, P. (2020). Diversity of left-right symmetry Cheng, S., Pei, Y., He, L., Peng, G., Reinius, B., Tam, P., Jing, breaking strategy in animals. F1000Research, 9, 123. <a N., Deng, Q. (2019). Single-Cell RNA-Seq Reveals Cellular href="http://dx.doi.org/10.12688/f1000research.21670.1">[Mor Heterogeneity of Pluripotency Transition and X Chromosome e Information]</a> Dynamics during Early Mouse Development. Cell Reports, 26(10), 2593-2607.e3. <a Salehin, N., Tam, P., Osteil, P. (2020). Prenet: Predictive href="http://dx.doi.org/10.1016/j.celrep.2019.02.031">[More network from ATAC-SEQ data. Journal of Bioinformatics and Information]</a> Computational Biology, 18(1), 2040003. <a href="http://dx.doi.org/10.1142/S021972002040003X">[More Humphreys, D., Fossat, N., Demuth, M., Tam, P., Ho, J. (2019). Information]</a> Ularcirc: visualization and enhanced analysis of circular RNAs via back and canonical forward splicing. Nucleic Acids Kim, H., Osteil, P., Humphrey, S., Cinghu, S., Oldfield, A., Research, 47(20), 1-17. <a Patrick, E., Wilkie, E., Peng, G., Suo, S., Jothi, R., Tam, P., href="http://dx.doi.org/10.1093/nar/gkz718">[More Yang, P. (2020). Transcriptional network dynamics during the Information]</a> progression of pluripotency revealed by integrative statistical learning. Nucleic Acids Research, 48(4), 1828-1842. <a 2018 href="http://dx.doi.org/10.1093/nar/gkz1179">[More Information]</a> Sibbritt, T., Ip, C., Khoo, P., Wilkie, E., Jones, V., Sun, J., Shen, J., Peng, G., Han, J., Jing, N., Osteil, P., Tam, P., Fossat, Fan, X., Waardenberg, A., Demuth, M., Osteil, P., Sun, J., N., et al (2018). A gene regulatory network anchored by LIM Loebel, D., Graham, M., Tam, P., Fossat, N. (2020). Twist1 homeobox 1 for embryonic head development. Genesis, 56(9), homodimers and heterodimers orchestrate lineage-specific 1-13. <a href="http://dx.doi.org/10.1002/dvg.23246">[More differentiation. Molecular and Cellular Biology, 40(11). <a Information]</a> href="http://dx.doi.org/10.1128/MCB.00663-19">[More Information]</a> Rossant, J., Tam, P. (2018). Exploring early human embryo development. Science, 360(6393), 1075-1076. <a differentiation. Cell Stem Cell, 20(1), 18-28. <a href="http://dx.doi.org/10.1126/science.aas9302">[More href="http://dx.doi.org/10.1016/j.stem.2016.12.004">[More Information]</a> Information]</a> McDonald, M., Morse, A., Schindeler, A., Mikulec, K., Zamir, L., Singh, R., Nathan, E., Patrick, R., Yifa, O., Yahalom- Peacock, L., Cheng, T., Bobyn, J., Lee, L., Baldock, P., Ronen, Y., Arraf, A., Schultheiss, T., Suo, S., Han, J., Tam, P., Croucher, P., Tam, P., Little, D. (2018). Homozygous Dkk1 et al (2017). Nkx2.5 marks angioblasts that contribute to Knockout Mice Exhibit High Bone Mass Phenotype Due to hemogenic endothelium of the endocardium and dorsal aorta. Increased Bone Formation. Calcified Tissue International, eLife, 6, 1-31. <a 102(1), 105-116. <a href="http://dx.doi.org/10.1007/s00223- href="http://dx.doi.org/10.7554/eLife.20994">[More 017-0338-4">[More Information]</a> Information]</a> Cui, G., Suo, S., Wang, R., Qian, Y., Han, J., Peng, G., Tam, P., Savatier, P., Osteil, P., Tam, P. (2017). Pluripotency of embryo- Jing, N. (2018). Mouse gastrulation: Attributes of transcription derived stem cells from rodents, lagomorphs, and primates: factor regulatory network for epiblast patterning. Development, Slippery slope, terrace and cliff. Stem Cell Research, 19, 104- Growth and Differentiation, 60(8), 463-472. <a 112. <a href="http://dx.doi.org/10.1111/dgd.12568">[More href="http://dx.doi.org/10.1016/j.scr.2017.01.008">[More Information]</a> Information]</a> Yu, Z., Tang, P., Wang, J., Bao, S., Shieh, J., Leung, A., Zhang, Chiang, I., Fritzsche, M., Thievend, C., Neal, A., Holmes, K., Z., Gao, F., Wong, S., Hui, A., Tam, P. (2018). Mutations in Lagendijk, A., Overman, J., D'Angelo, D., Omini, A., Tam, P. Hnrnpa1 cause congenital heart defects. JCI Insight, 3(2), 1-21. (2017). SoxF factors induce Notch1 expression via direct <a href="http://dx.doi.org/10.1172/jci.insight.98555">[More transcriptional regulation during early arterial development. Information]</a> Development, 144(20), 3847-3848. <a href="http://dx.doi.org/10.1242/dev.159715">[More Friedman, C., Nguyen, Q., Lukowski, S., Helfer, A., Chiu, H., Information]</a> Miklas, J., Levy, S., Suo, S., Han, J., Osteil, P., Tam, P., et al (2018). Single-Cell Transcriptomic Analysis of Cardiac Chen, J., Suo, S., Tam, P., Han, J., Peng, G., Jing, N. (2017). Differentiation from Human PSCs Reveals HOPX-Dependent Spatial transcriptomic analysis of cryosectioned tissue samples Cardiomyocyte Maturation. Cell Stem Cell, 23(4), 586-598. <a with Geo-seq. Nature Protocols, 12(3), 566-580. <a href="http://dx.doi.org/10.1016/j.stem.2018.09.009">[More href="http://dx.doi.org/10.1038/nprot.2017.003">[More Information]</a> Information]</a> Liu, C., Wang, R., He, Z., Osteil, P., Wilkie, E., Yang, X., 2016 Chen, J., Cui, G., Guo, W., Chen, Y., Tam, P., et al (2018). Suppressing Nodal Signaling Activity Predisposes Ectodermal Zheng, G., Zhang, J., Zhao, H., Wang, H., Pang, M., Qiao, X., Differentiation of Epiblast Stem Cells. Stem Cell Reports, Lee, S., Hsu, T., Tan, T., Lyons, J., Zhao, Y., Loebel, D., Wang, 11(1), 43-57. <a Y., Wang, Y., Wang, Y., Cao, Q., Wang, C., Lee, V., Tam, P., href="http://dx.doi.org/10.1016/j.stemcr.2018.05.019">[More Harris, D., et al (2016). a3 Integrin of Cell-Cell Contact Information]</a> Mediates Kidney Fibrosis by Integrin-Linked Kinase in Proximal Tubular E-Cadherin Deficient Mice. American 2017 Journal of Pathology, 186(7), 1847-1860. <a href="http://dx.doi.org/10.1016/j.ajpath.2016.03.015">[More Yang, J., Ryan, D., Wang, W., Tsang, J., Lan, G., Masaki, H., Information]</a> Gao, X., Antunes, L., Yu, Y., Tam, P., et al (2017). Establishment of mouse expanded potential stem cells. Nature, Kondo, M., Gray, L., Pelka, G., Leang, S., Christodoulou, J., 550(7676), 393-397. <a Tam, P., Hannan, A. (2016). Affective dysfunction in a mouse href="http://dx.doi.org/10.1038/nature24052">[More model of Rett syndrome: Therapeutic effects of environmental Information]</a> stimulation and physical activity.
Load more

Recommended publications
  • AIRE Is a Critical Spindle-Associated Protein in Embryonic Stem Cells Bin Gu1, Jean-Philippe Lambert2, Katie Cockburn1, Anne-Claude Gingras2,3, Janet Rossant1,3*
    RESEARCH ARTICLE AIRE is a critical spindle-associated protein in embryonic stem cells Bin Gu1, Jean-Philippe Lambert2, Katie Cockburn1, Anne-Claude Gingras2,3, Janet Rossant1,3* 1Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, Canada; 2Lunenfeld-Tanenbaum Research Institute at Mount Sinai Hospital, Toronto, Canada; 3Department of Molecular Genetics, University of Toronto, Toronto, Canada Abstract Embryonic stem (ES) cells go though embryo-like cell cycles regulated by specialized molecular mechanisms. However, it is not known whether there are ES cell-specific mechanisms regulating mitotic fidelity. Here we showed that Autoimmune Regulator (Aire), a transcription coordinator involved in immune tolerance processes, is a critical spindle-associated protein in mouse ES(mES) cells. BioID analysis showed that AIRE associates with spindle-associated proteins in mES cells. Loss of function analysis revealed that Aire was important for centrosome number regulation and spindle pole integrity specifically in mES cells. We also identified the c-terminal LESLL motif as a critical motif for AIRE’s mitotic function. Combined maternal and zygotic knockout further revealed Aire’s critical functions for spindle assembly in preimplantation embryos. These results uncovered a previously unappreciated function for Aire and provide new insights into the biology of stem cell proliferation and potential new angles to understand fertility defects in humans carrying Aire mutations. DOI: 10.7554/eLife.28131.001 *For correspondence: janet. [email protected] Introduction Competing interests: The Self-renewal capability, defined as the ability of cells to proliferate while sustaining differentiation authors declare that no potential, is one of the defining features of stem cells (Martello and Smith, 2014).
    [Show full text]
  • Beacon in Developmental Biology
    NEWS Beacon in developmental biology n Cite as: CMAJ 2017 October 16;189:E1298-9. doi: 10.1503/cmaj.109-5460 Posted on cmajnews.com on September 27, 2017. he identification of Gurdon, whom some people the genes responsi- may know recently received ble for controlling the Nobel Prize for his work Tcellular fate and embryonic cloning frogs; undertaking development in mice has research that demonstrates made Dr. Janet Rossant a that all of development is beacon in the field of written in our genes, and all developmental biology. of our cells contain DNA that Her work pioneered tech- has that information, yet niques that allowed genes somehow during develop- to be altered within ment, different genes are embryos. It also acceler- turned on and differentiation ated avenues in the fields occurs. That’s what really of stem cell biology and turned me on to the underly- regenerative medicine. ing problem of ‘how do we She was awarded the get from a fertilized egg to an 2015 Canada Gairdner organism?’ John is the per- Wightman Award, pre- son who really turned me on sented annually to a Cana- to that question and he has dian who has demon- been pursuing it ever since strated outstanding and so have I.” leadership in medicine and medical science. Rossant For the diverse audience was recognized “for her who read CMAJ, can you outstanding contributions explain what stem cells to developmental biology are? and for her exceptional “Stem cells are cells that international leadership in have the ability to self- stem cell biology and pol- renew, make endless copies icy-making, and in advanc- of themselves and yet also ing research programs for retain the potential to differ- children’s illnesses.” Courtesy of Dr.
    [Show full text]
  • Annual Report Fy 2018 Human Frontier Science Program Organization
    APRIL 2017 APRIL 2018 — MARCH 2019 ANNUAL REPORT FY 2018 HUMAN FRONTIER SCIENCE PROGRAM ORGANIZATION The Human Frontier Science Program Organization (HFSPO) is unique, supporting international collaboration to undertake innovative, risky, basic research at the frontier of the life sciences. Special emphasis is given to the support and training of independent young investigators, beginning at the postdoctoral level. The Program is implemented by an international organisation, supported financially by Australia, Canada, France, Germany, India, Italy, Japan, the Republic of Korea, New Zealand, Norway, Singapore, Switzerland, the United Kingdom of Great Britain and Nothern Ireland, the United States of America, and the European Commission. Since 1990, over 7000 researchers from more than 70 countries have been supported. Of these, 28 HFSP awardees have gone on to receive the Nobel Prize. 2 The following documents are available on the HFSP website www.hfsp.org: Joint Communiqués (Tokyo 1992, Washington 1997, Berlin 2002, Bern 2004, Ottawa 2007, Canberra 2010, Brussels 2013, London 2016): https://www.hfsp.org/about/governance/membership Statutes of the International Human Frontier Science Program Organization: https://www.hfsp.org/about/governance/hfspo-statutes Guidelines for the participation of new members in HFSPO: https://www.hfsp.org/about/governance/membership General reviews of the HFSP (1996, 2001, 2006-2007, 2010, 2018): https://www.hfsp.org/about/strategy/reviews Updated and previous lists of awards, including titles and abstracts:
    [Show full text]
  • Balancing Potency and Differentiation in Mouse Embryo-Derived Stem Cells and in Vivo
    Balancing potency and differentiation in mouse embryo-derived stem cells and in vivo By Anne Christine Helness A thesis submitted to Imperial College London for the degree of Doctor of Philosophy Department of Surgery and Oncology Imperial College London September 2011 1 Abstract An inherent challenge for developing organisms is to maintain a critical balance between cell potency and differentiation. This is most obvious in embryonic stem (ES) cells where genetic, epigenetic and cell signalling pathways support ES cell ability to self-renew and to generate all embryonic lineages. Recently, a series of reports revealed how Polycomb-mediated repression might buffer the precocious expression of somatic lineage regulators in ES cells. Notably, these genes carry bivalent chromatin enriched in both repressive (H3K27me3) and permissive (H3K4me2) histone marks. They are targeted by the Polycomb repressive complexes PRC1 (Ring1B) and PRC2, which mediates H3K27me3 and assemble poised RNA polymerase II (RNAP II), conferring silencing of loci primed for future activation (or repression) upon ES cell differentiation. During early development the transition from morula to blastocyst is the starting point for lineage segregation into the inner cell mass (ICM) and trophectoderm (TE). ES cells are derived from the ICM and are pluripotent. By contrast, TE-derived trophoblast stem (TS) cells are multipotent and contribute solely to placenta formation in vivo. To address whether ES cells epigenetic features are unique attributes of pluripotent cells in the early embryo, we compared the epigenetic status of key developmental genes in blastocyst-derived stem cells and in vivo. We provide direct evidence that bivalent histone markings operate in vivo from the eight-cell up to the blastocyst stage.
    [Show full text]
  • Targeting SARS-Cov-2 Entry Title
    Genomics-guided identication of potential modulators of SARS-CoV-2 entry proteases, TMPRSS2 and Cathepsins B/L Kartikay Prasad Amity Institute of Neuropsychology & Neurosciences, Amity University, Noida Vijay Kumar ( [email protected] ) Amity Institute of Neuropsychology & Neurosciences, Amity University, Noida https://orcid.org/0000- 0002-3621-5025 Research Article Keywords: COVID-19, TMPRSS2, Cathepsins, gene set enrichment, estradiol, retinoic acid Posted Date: January 1st, 2021 DOI: https://doi.org/10.21203/rs.3.rs-138273/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Running Title: Targeting SARS-CoV-2 entry Title: Genomics-guided identification of potential modulators of SARS-CoV-2 entry proteases, TMPRSS2 and Cathepsins B/L Kartikay Prasad1, and Vijay Kumar1* 1Amity Institute of Neuropsychology & Neurosciences, Amity University, Noida, UP- 201303, India *Address for Correspondence Vijay Kumar, Ph. D. Assistant Professor Amity Institute of Neuropsychology & Neurosciences, Amity University, Noida, UP- 201313, India E-mail: [email protected] ORCID: 0000-0002-3621-5025 1 Abstract The entry of SARS-CoV-2 into host cells requires the activation of its spike protein by host cell proteases. The serine protease, transmembrane serine protease 2 (TMPRSS2) and cysteine proteases, cathepsins B, L (CTSB/L) activate spike protein and enabling SARS-CoV-2 entry to the host cell through two completely different and independent pathways. Given that the uncertainty of how SARS-CoV-2 infects and kills, the need for a deep understanding of SARS- CoV-2 biology is imperative. Herein, we performed genomic-guided meta-analysis to identify upstream regulatory elements altering the expression of TMPRSS2 and CTSB/L genes.
    [Show full text]
  • EMBO Encounters Issue43.Pdf
    WINTER 2019/2020 ISSUE 43 Nine group leaders selected Meet the first EMBO Global Investigators PAGE 6 Accelerating scientific publishing EMBO publishing costs Review Commons Making our journals’ platform announced finances public PAGE 3 PAGES 10 – 11 Welcome, Young Investigators! Contract replaces stipend Marking ten years 27 group leaders join the programme EMBO Postdoctoral Fellowships EMBO Molecular Medicine receive an update celebrates anniversary PAGES 4 – 5 PAGE 7 PAGE 13 www.embo.org TABLE OF CONTENTS EMBO NEWS EMBO news Review Commons: accelerating publishing Page 3 EMBO Molecular Medicine turns ten © Marietta Schupp, EMBL Photolab Marietta Schupp, © Page 13 Editorial MBO was founded by scientists for Introducing 27 new Young Investigators scientists. This philosophy remains at Pages 4-5 Ethe heart of our organization until today. EMBO Members are vital in the running of our Meet the first EMBO Global programmes and activities: they screen appli- Accelerating scientific publishing 17 journals on board Investigators cations, interview candidates, decide on fund- Review Commons will manage the transfer of ing, and provide strategic direction. On pages EMBO and ASAPbio announced pre-journal portable review platform the manuscript, reviews, and responses to affili- Page 6 8-9 four members describe why they chose to ate journals. A consortium of seventeen journals New members meet in Heidelberg dedicate their time to an EMBO Committee across six publishers (see box) have joined the Fellowships: from stipends to contracts Pages 14 – 15 and what they took away from the experience. n December 2019, EMBO, in partnership with decide to submit their work to a journal, it will project by committing to use the Review Commons Page 7 When EMBO was created, the focus lay ASAPbio, launched Review Commons, a multi- allow editors to make efficient editorial decisions referee reports for their independent editorial deci- specifically on fostering cross-border inter- Ipublisher partnership which aims to stream- based on existing referee comments.
    [Show full text]
  • Dr. Janet Rossant ANDREW F. HOLMES DEAN of MEDICINE DISTINCTION LECTURES
    Dr. Janet Rossant ANDREW F. HOLMES DEAN OF MEDICINE DISTINCTION LECTURES Dr. Janet Rossant, PhD, FRS, FRSC Chief of Research, SickKids Senior Scientist, Developmental & Stem Cell Biology, SickKids Lombard Insurance Chair in Pediatric Research University Professor, Department of Molecular Genetics, Obstetrics and Gynecology, University of Toronto Title Stem cells and early lineage development When Friday, October 18, 2013 11:30 a.m. – 1:00 p.m. Where CP Leblond Amphitheatre Strathcona Anatomy and Dentistry Building 3640 rue University Montreal, QC H3G 1Y6 Dr. Janet Rossant, SickKids Chief of Research and a world-renowned expert in developmental biology, is the definition of a trailblazer. Widely known for her studies of the genes that control embryonic development in the mouse, Dr. Rossant has pioneered techniques for following cell fate and altering genes in embryos. This work continues to resonate in medical genetic research. Her current research focuses on stem cell development and cell differentiation in the developing embryo, important areas for the study of birth defects as well as regenerative medicine. Firmly planted on the front lines of technological change, Dr. Rossant has established SickKids as a global forerunner in genetic research. Janet Rossant was born in Chatham, UK, and trained at the Universities of Oxford and Cambridge, United Kingdom. In 1977, Dr. Rossant moved to Canada and joined the faculty at Brock University. From 1985 to 2005, she was a leading researcher at the Samuel Lunenfeld Research Institute at Mount Sinai Hospital in Toronto. She joined SickKids in 2005 and became Chief of Research of the Research Institute. She is also a University Professor at the University of Toronto.
    [Show full text]
  • Web Conference with Dr. Janet Rossant
    Web Conference with Dr. Janet Rossant - 00:00 Thank you for taking the time to speak with us, so obviously we'd like to ask you some questions about your work and your research, but can you tell us about yourself and your research, how you got into stem cells in the first place? - 00:16 Okay, so I've been interested in early development for a very long time, sort of all my research career. I started as a graduate student at the University of Cambridge with Richard Gardner who was one of the first people who developed techniques to inject cells into blastocysts to make chimeras, and so I was always interested in how an egg develops into a complex organism, and over the years I've worked on the cell lineage in the early embryo. We've then been able to move in and identify some of the genes and pathways that specifies cell fate in the early embryo, and of course along the way, stem cells come into the picture because it's from the early mammalian embryo, the blastocysts, that you can derive embryonic stem cells, and it's really the work that I and my colleagues did early on in the embryo showing that there was a subset of cells that were pluripotent that gave people the idea that maybe you could capture that pluripotency and derive permanent cell lines from those cells and grow them in cultures. So we've worked on mouse embryonic stem cells, but we also have been able to show that we can derive stem cells from the other two types of the blastocysts, the trophoblast and the primitive endoderm, so in the mouse you can have three stem cell lines from the blastocysts, each of which replicates the properties of those lineages in vivo.
    [Show full text]
  • Pnas11052ackreviewers 5098..5136
    Acknowledgment of Reviewers, 2013 The PNAS editors would like to thank all the individuals who dedicated their considerable time and expertise to the journal by serving as reviewers in 2013. Their generous contribution is deeply appreciated. A Harald Ade Takaaki Akaike Heather Allen Ariel Amir Scott Aaronson Karen Adelman Katerina Akassoglou Icarus Allen Ido Amit Stuart Aaronson Zach Adelman Arne Akbar John Allen Angelika Amon Adam Abate Pia Adelroth Erol Akcay Karen Allen Hubert Amrein Abul Abbas David Adelson Mark Akeson Lisa Allen Serge Amselem Tarek Abbas Alan Aderem Anna Akhmanova Nicola Allen Derk Amsen Jonathan Abbatt Neil Adger Shizuo Akira Paul Allen Esther Amstad Shahal Abbo Noam Adir Ramesh Akkina Philip Allen I. Jonathan Amster Patrick Abbot Jess Adkins Klaus Aktories Toby Allen Ronald Amundson Albert Abbott Elizabeth Adkins-Regan Muhammad Alam James Allison Katrin Amunts Geoff Abbott Roee Admon Eric Alani Mead Allison Myron Amusia Larry Abbott Walter Adriani Pietro Alano Isabel Allona Gynheung An Nicholas Abbott Ruedi Aebersold Cedric Alaux Robin Allshire Zhiqiang An Rasha Abdel Rahman Ueli Aebi Maher Alayyoubi Abigail Allwood Ranjit Anand Zalfa Abdel-Malek Martin Aeschlimann Richard Alba Julian Allwood Beau Ances Minori Abe Ruslan Afasizhev Salim Al-Babili Eric Alm David Andelman Kathryn Abel Markus Affolter Salvatore Albani Benjamin Alman John Anderies Asa Abeliovich Dritan Agalliu Silas Alben Steven Almo Gregor Anderluh John Aber David Agard Mark Alber Douglas Almond Bogi Andersen Geoff Abers Aneel Aggarwal Reka Albert Genevieve Almouzni George Andersen Rohan Abeyaratne Anurag Agrawal R. Craig Albertson Noga Alon Gregers Andersen Susan Abmayr Arun Agrawal Roy Alcalay Uri Alon Ken Andersen Ehab Abouheif Paul Agris Antonio Alcami Claudio Alonso Olaf Andersen Soman Abraham H.
    [Show full text]
  • Final Program
    FINAL PROGRAM TRANSLATING REPRODUCTIVE SCIENCE TO THE BEDSIDE Vancouver Convention Centre East 999 Canada Pl Vancouver, BC, Canada V6C 3C1 SRI-1019-633 The SRI 67th Annual Scientific Meeting is an Accredited Group Learning Activity (Section 1) as defined by the Maintenance of Certification Program of the Royal College of Physicians and Surgeons of Canada, and approved by Society of Obstetricians and Gynaecologists of Canada. VANCOUVER MARCH 10-14, 2020 - Vancouver Convention Centre East 1 Table of Contents Message from the SRI President . 1 SRI Membership Recognition Program Ads . 2 SRI Mentor Hall of Fame . 5 2020 Program Committee . 6 General Meeting Information . 7 Meeting Attendance Code of Conduct Policy . 8 Schedule-at-a-Glance . 9 Social Events . 10 Exhibitors . 11 Vancouver Convention Centre East and Host Hotels Map . 12 Vancouver Convention Centre East Level 1 Map . 13 Vancouver Convention Centre East Level 2 Map . 14 Continuing Medical Education Information . 15 Scientific Program . 16 Thank You to Our 2020 Poster Judges . 60 SRI Grant Opportunities . .61 2020 Awards . 62 About SRI…………………………… . 64 2019 – 2020 Annual Report . 66 SRI Council & Staff . 68 Poster Guide . 69 Oral and Poster Abstract Author Index . 108 Notes . 131 Thank You to Our 2020 Supporters . Inside. Back Cover Save the Date: SRI 2021 Annual Meeting . Back Cover SRI 67th Annual Scientific Meeting Venue Vancouver Convention Centre East 999 Canada Pl Vancouver, BC V6C 3C1 Canada SRI 67th Annual Scientific Host Hotels Pan Pacific Hotel Vancouver • 300-999 Canada
    [Show full text]
  • Cloning Human Beings
    CLONING HUMAN BEINGS The Science of Animal Cloning Commissioned Paper by Janet Rossant, Ph.D. Samuel Lunenfield Research Institute-Mount Sinai Hospital CONTENTS What Is Cloning? B-3 The Scientific Question: Does Differentiation Involve Irreversible Changes in Genetic Content? B-4 The Stability of the Differentiated State: Our Understanding Today B-6 Nuclear Transfer in Mammals: The Early Experiments B-7 Reprogramming in the Oocyte Environment B-8 Nuclear Transfer in Mammals: The Current State of Play B-9 And Then Came Dolly B-10 Why Pursue Animal Cloning Research? B-12 1. Making Clones for Research Purposes B-12 2. Propagating Desirable Stocks B-13 3. Improved Generation and Propagation of Transgenic Livestock B-13 4. Generating Targeted Gene Alterations B-14 How Can We Use Information from Nuclear Transfer Experiments for Human Benefit? B-17 Ethical Concerns B-17 References B-18 B-1 WHAT IS CLONING? The word “clone” is used in many different contexts in biological research, but in simple terms it means a set of genetically identical individuals. Scientists talk about cloning DNA—the process of making and propagating a set of identical copies of a particular piece of genetic material, or about cloning cells—taking a single cell in culture and allowing it to multiply into a cell line. These techniques are some of the basic tools of the trade of modern biomedical research and are not currently a source of much public concern. But when we talk about cloning whole animals, especially mammals, the public rightly wants to know what is going on and why.
    [Show full text]
  • SOX17 Increases the Cisplatin Sensitivity of an Endometrial Cancer
    Zhang et al. Cancer Cell Int (2016) 16:29 DOI 10.1186/s12935-016-0304-7 Cancer Cell International PRIMARY RESEARCH Open Access SOX17 increases the cisplatin sensitivity of an endometrial cancer cell line Yongli Zhang1,3, FeiZhou Jiang1, Wei Bao2, Huilin Zhang1, XiaoYing He2, Huihui Wang1 and Xiaoping Wan3* Abstract Background: Endometrial cancer (EC) is the most common form of malignant gynecological tumor. Treatment with cisplatin (CDDP) is the mainstay of EC chemotherapy. The apoptotic machinery is regarded as an important etiologi- cal factor in chemoresistance. Recent evidence has suggested that overexpression of the transcription factor SOX17 prevented apoptosis in tumor cell lines. The effect of SOX17 on apoptosis in EC cisplatin chemoresistance remains unclear. Methods: Immunohistochemistry and the reverse transcription-polymerase chain reaction were employed to detect gene expression in paraffin-embedded EC tissues and blood samples. The anti-proliferative ability of SOX17 on EC cells was assessed by MTT. Flow cytometric analysis was used to detect cell apoptosis by annexin V/PI double- staining. The expression of apoptosis-related proteins was analyzed by western blot. In the in vivo study, nude mice were subcutaneously injected with EC cells, and received cisplatin treatment through intraperitoneal chemotherapy. Apoptosis of in vivo samples was analyzed by TUNEL assay. Results: SOX17 expression decreased the chemical resistance of EC cells to CDDP. HEC-1B cells with an elevated expression of SOX17 had a lower cell viability and higher apoptosis rate after cisplatin exposure. Overexpression SOX17 up-regulated wild type p53 after being exposed to cisplatin, while the expression of BCL2-associated X protein and cleaved caspase-3 simultaneously increased.
    [Show full text]