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Usbiological Datasheet PLA2G5, CT (PLA2G5, Calcium-dependent phospholipase A2, Group V phospholipase A2, PLA2-10, Phosphatidylcholine 2-acylhydrolase 5) (FITC) Catalog number 040120-FITC Supplier United States Biological This gene is a member of the secretory phospholipase A2 family. It is located in a tightly-linked cluster of secretory phospholipase A2 genes on chromosome 1. The encoded enzyme catalyzes the hydrolysis of membrane phospholipids to generate lysophospholipids and free fatty acids including arachidonic acid. It preferentially hydrolyzes linoleoyl-containing phosphatidylcholine substrates. Secretion of this enzyme is thought to induce inflammatory responses in neighboring cells. Alternatively spliced transcript variants have been found, but their full-length nature has not been determined. [provided by RefSeq]. Applications Suitable for use in Western Blot, Immunohistochemistry and FLISA. Other applications have not been tested. Recommended Dilution Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher. Storage and Stability Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note Applications are based on unconjugated antibody. Immunogen KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 103-132 in the C-terminal region of human PLA2G5. Formulation Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC). Purity Purified by Protein A and peptide affinity chromatography. Specificity Recognizes human PLA2G5. Product Type Pab Source human Isotype IgG Grade Affinity Purified Applications FLISA IHC WB Crossreactivity Hu Storage -20°C Detection Method FITC Reference 1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) 2. Chattopadhyay, I., et al. Mutat. Res. 696(2):130-138(2010) 3. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009) 4. Wootton, P.T., et al. Hum. Mol. Genet. 16(12):1437-1444(2007) 5. de Beer, F.C., et al. Arterioscler. Thromb. Vasc. Biol. 26(7):1421-1422(2006) Powered by TCPDF (www.tcpdf.org).
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