A Recommendation of the Cancer Moonshot Blue Ribbon Panel

HTANTHE HUMAN TUMOR ATLAS NETWORK

Building Dynamic 3D Human Tumor Atlases to Advance Cancer Research

Blue Ribbon Panel’s NCI launched the Human Tumor Atlas space and time scales. By providing Recommendation: Network (HTAN). This collaborative insights about the composition, network is using emerging technologies structure, and function of the various Develop 3D Cancer Atlases to create dynamic 3D human tumor cells within the evolving tumor atlases at single-cell resolution. Like ecosystem, these human tumor atlases Tumors are ecosystems that Google Earth, these atlases can be will enable predictive modeling to continuously evolve. Despite advances viewed as interactive 3D maps showing inform cancer treatment and in understanding interactions within the features of a tumor across multiple prevention options for cancer patients. the cancer ecosystem, we know little about how the tumor architecture reflects progression from WHY DO WE NEED HUMAN TUMOR ATLASES? premalignant lesions to metastasis or • Define the cellular and molecular architecture of human tumors how resistance to therapy develops. • Model tumor progression from pre-cancer to metastasis To address this challenge, the Cancer MoonshotSM Blue Ribbon Panel • Understand the pathways that lead to therapy resistance recommended the generation of • Develop a community resource for cancer research comprehensive human tumor atlases that describe the molecular, cellular, • Enable the development of predictive models for cancer patients structural, and morphological characteristics of cancers over time. In response to this recommendation,

U.S. Department of Health & Human Services | National Institutes of Health What is a Human Tumor Atlas?

A comprehensive human tumor atlas is that are sensitive or resistant to function of individual cells within the HOW HTAN IS ORGANIZED atlases of cancers and pre-cancers. network. The HTAN Data Coordinating defined as a multidimensional immunotherapy, hereditary cancers, complex tumor environment. This Additionally, two HTAN Pilot Projects Center manages the sharing of data HTAN consists of collaborative, molecular, cellular, and morphological and aggressive pediatric cancers. This information is expected to advance are developing approaches and and compilation of atlases generated interdisciplinary research teams from survey of human cancers over space involves the construction of atlases basic and translational cancer research, standard operating procedures for by the network. This center also makes institutions across the country. Ten and time. It can be thought of as a representing melanoma and cancers of such as the identification of biomarkers tumor tissue collection and data HTAN community resources available Human Tumor Atlas Research Centers virtual 3D map of an evolving tumor the lung, pancreas, breast, and colon in for cancer prevention and early analysis that will be used across the to the broader research community. of the network are creating detailed 3D ecosystem that shows the locations of adults, as well as brain cancers, detection. individual molecules, cells, and tissues. neuroblastoma, sarcoma, and very high HOW HTAN CONTRIBUTES TO A human tumor atlas provides risk acute lymphoblastic leukemia in There are other altas-building initiatives HTAN GOALS THE CANCER MOONSHOT visualization of the structure, children. The atlases will represent a in biomedical research, and HTAN HTAN is part of the Cancer Moonshot, composition, and multiscale diverse population, including minority complements, yet is also distinct from, • Generate publicly available dynamic 3D human tumor atlases which aims to accelerate progress in interactions as a tumor grows, and underserved patients with cancer. these projects. HTAN is unique in that it • Enable predictive modeling of cancer across space and time cancer research, encourage metastasizes, or responds to cancer focuses on a range of cancers and • Promote aggressive public data and resource release timelines collaboration, and enhance data therapy. In order to create human tumor examines how the spatial interactions sharing. HTAN is a collaborative atlases, HTAN researchers need to of tumor components change over • Identify emerging technologies that will inform cancer research network that is contributing to these Like geographical mapping websites or collect and integrate single-cell time. This is achieved through longitu- • Develop standard operating procedures for future atlas efforts goals by generating resources, apps that predict the best route to a resolution data from tumors. HTAN dinal sample collection and extensive including atlases, data, technologies, new location and account for changing researchers are leveraging analysis of clinical data that, together, and publications, that will be shared travel conditions, detailed human technological advances to will inform our understanding of with the research community. These tumor atlases could be used to predict comprehensively analyze single cells disease progression and therapy resources will catalyze novel studies of cancer patient responses to a within a cancer ecosystem. Unlike resistance. cancer and inform future atlas building treatment or to identify individuals for previous efforts, HTAN tumor atlases efforts. Ultimately, the human tumor cancer prevention who are at increased take into account the spatial atlases from HTAN will enable risk for cancer. information of components within the predictive modeling to improve clinical tumor. Since many single-cell choices for individual patients. HOW HTAN IS BUILDING technologies require the dismantling of HUMAN TUMOR ATLASES tumors which results in a loss of spatial HTAN is constructing dynamic 3D relationships, HTAN investigators are tumor atlases that describe important using emerging, spatial imaging technologies to study the intact tumor “Single-cell transitions in cancer. The network is specifically focusing on the evolution architecture. By combining individual technologies are from the precancerous state to data from hundreds to thousands of malignancy, the progression from a single cells with spatial information opening an entire localized tumor to metastatic disease, about the cellular architecture of new world for cancer the change in tumors during response tumors, researchers can to therapy, and the development of determine the location and and biomedicine. resistance to treatment.

The Human Tumor Atlas Network Dana Pe'er, Ph.D. focuses on high-risk cancers: tumors “ Memorial Sloan Kettering Cancer Center HTAN Investigator HTAN Consortium

LUNG BREAST Avrum Spira & Steven Dubinett Shelley Hwang, Carlo Maley, Boston University & University of California Los & Robert West Angeles Duke University, Arizona State University, & Stanford University Dana Pe’er & Christine Icabuzio-Donahue Memorial Sloan-Kettering Cancer Center Joe Gray, Gordon Mills, Jeremy Goecks, & Christopher Corless Molecular and Cellular Characterization Oregon Health & Science University of Screen Detected Lesions (MCL) Consortium Pre-Cancer Atlas Pilot Bruce Johnson & Aviv Regev Dana-Farber Cancer Institute & Broad Institute

Li Ding, Ryan Fields, William Gillanders, & Samuel Achilefu PANCREAS Washington University in St. Louis Dana Pe’er & Christine Iacobuzio-Donahue Frederick National Laboratory for Cancer Memorial Sloan-Kettering Cancer Center Research (FNLCR) & Broad Institute MCL Consortium Pre-Cancer Atlas Pilot Tumor Atlas Pilot Li Ding, Ryan Fields, William Gillanders, MCL Consortium Pre-Cancer Atlas Pilot & Samuel Achilefu Washington University in St. Louis COLON Michael Snyder & James Ford Stanford University PEDIATRIC Robert Coffey, Ken Lau, FNLCR & Broad Institute & Martha Shrubsole Tumor Atlas Pilot Vanderbilt University Glioma, neuroblastoma, and sarcoma (organs commonly affected by these Bruce Johnson & Aviv Regev cancers include the brain, adrenal glands, Dana-Farber Cancer Institute and muscle) & Broad Institute

Kai Tan and Stephen Hunger SKIN Children's Hospital of Philadelphia Peter Sorger, Sandro Santagata, Glioma, neuroblastoma, and very high risk & Jon Aster acute lymphoblastic leukemia (organs Harvard University & Brigham commonly affected by these cancers and Women’s Hospital include the brain, adrenal glands, and blood) Bruce Johnson & Aviv Regev Dana-Farber Cancer Institute & Broad Institute

HTAN DATA COORDINATING CENTER (DCC)

Ethan Cerami, Justin Guinney, Nikolaus Shultz, & Vésteinn Thorsson Dana-Farber Cancer Institute, Sage Bionetworks, Memorial Sloan Kettering Cancer Center, & Institute for Systems Biology

LEARN MORE ABOUT HTAN Find information about HTAN community resources, including 3D human tumor atlases: humantumoratlas.org | cancer.gov/htan ˜ @NCIHTAN | cancer.gov/brp