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An Aerobic Eukaryotic Parasite with Functional Mitochondria That Likely
An aerobic eukaryotic parasite with functional mitochondria that likely lacks a mitochondrial genome Uwe John, Yameng Lu, Sylke Wohlrab, Marco Groth, Jan Janouškovec, Gurjeet Kohli, Felix Mark, Ulf Bickmeyer, Sarah Farhat, Marius Felder, et al. To cite this version: Uwe John, Yameng Lu, Sylke Wohlrab, Marco Groth, Jan Janouškovec, et al.. An aerobic eukaryotic parasite with functional mitochondria that likely lacks a mitochondrial genome. Science Advances , American Association for the Advancement of Science (AAAS), 2019, 5 (4), pp.eaav1110. 10.1126/sci- adv.aav1110. hal-02372304 HAL Id: hal-02372304 https://hal.archives-ouvertes.fr/hal-02372304 Submitted on 25 Nov 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. SCIENCE ADVANCES | RESEARCH ARTICLE EVOLUTIONARY BIOLOGY Copyright © 2019 The Authors, some rights reserved; An aerobic eukaryotic parasite with functional exclusive licensee American Association mitochondria that likely lacks a mitochondrial genome for the Advancement Uwe John1,2*, Yameng Lu1,3, Sylke Wohlrab1,2, Marco Groth4, Jan Janouškovec5, Gurjeet S. Kohli1,6, of Science. No claim to 1 1 7 4 1,8 original U.S. Government Felix C. Mark , Ulf Bickmeyer , Sarah Farhat , Marius Felder , Stephan Frickenhaus , Works. -
Molecular Data and the Evolutionary History of Dinoflagellates by Juan Fernando Saldarriaga Echavarria Diplom, Ruprecht-Karls-Un
Molecular data and the evolutionary history of dinoflagellates by Juan Fernando Saldarriaga Echavarria Diplom, Ruprecht-Karls-Universitat Heidelberg, 1993 A THESIS SUBMITTED IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE FACULTY OF GRADUATE STUDIES Department of Botany We accept this thesis as conforming to the required standard THE UNIVERSITY OF BRITISH COLUMBIA November 2003 © Juan Fernando Saldarriaga Echavarria, 2003 ABSTRACT New sequences of ribosomal and protein genes were combined with available morphological and paleontological data to produce a phylogenetic framework for dinoflagellates. The evolutionary history of some of the major morphological features of the group was then investigated in the light of that framework. Phylogenetic trees of dinoflagellates based on the small subunit ribosomal RNA gene (SSU) are generally poorly resolved but include many well- supported clades, and while combined analyses of SSU and LSU (large subunit ribosomal RNA) improve the support for several nodes, they are still generally unsatisfactory. Protein-gene based trees lack the degree of species representation necessary for meaningful in-group phylogenetic analyses, but do provide important insights to the phylogenetic position of dinoflagellates as a whole and on the identity of their close relatives. Molecular data agree with paleontology in suggesting an early evolutionary radiation of the group, but whereas paleontological data include only taxa with fossilizable cysts, the new data examined here establish that this radiation event included all dinokaryotic lineages, including athecate forms. Plastids were lost and replaced many times in dinoflagellates, a situation entirely unique for this group. Histones could well have been lost earlier in the lineage than previously assumed. -
Parasitic Dinoflagellate Hematodinium Perezi Prevalence in Larval and Juvenile Blue Crabs Callinectes Sapidus from Coastal Bays of Virginia
W&M ScholarWorks VIMS Articles Virginia Institute of Marine Science 6-6-2019 Parasitic dinoflagellate Hematodinium perezi prevalence in larval and juvenile blue crabs Callinectes sapidus from coastal bays of Virginia HJ Small Virginia Institute of Marine Science JP Huchin-Mian Virginia Institute of Marine Science KS Reece Virginia Institute of Marine Science KM Pagenkopp Lohan MJ Butler IV See next page for additional authors Follow this and additional works at: https://scholarworks.wm.edu/vimsarticles Part of the Marine Biology Commons, and the Parasitology Commons Recommended Citation Small, HJ; Huchin-Mian, JP; Reece, KS; Pagenkopp Lohan, KM; Butler, MJ IV; and Shields, JD, Parasitic dinoflagellate Hematodinium perezi prevalence in larval and juvenile blue crabs Callinectes sapidus from coastal bays of Virginia (2019). Diseases of Aquatic Organisms, 134, 215-222. 10.3354/dao03371 This Article is brought to you for free and open access by the Virginia Institute of Marine Science at W&M ScholarWorks. It has been accepted for inclusion in VIMS Articles by an authorized administrator of W&M ScholarWorks. For more information, please contact [email protected]. Authors HJ Small, JP Huchin-Mian, KS Reece, KM Pagenkopp Lohan, MJ Butler IV, and JD Shields This article is available at W&M ScholarWorks: https://scholarworks.wm.edu/vimsarticles/1428 Vol. 134: 215–222, 2019 DISEASES OF AQUATIC ORGANISMS Published online June 6 https://doi.org/10.3354/dao03371 Dis Aquat Org OPENPEN ACCESSCCESS Parasitic dinoflagellate Hematodinium perezi prevalence in larval and juvenile blue crabs Callinectes sapidus from coastal bays of Virginia H. J. Small1,*, J. P. Huchin-Mian1,3, K. -
Blue Crab (Callinectes Sapidus) Use of the Ship/Trinity/Tiger Shoal
ACCESS NUMBER: 32806-35161 STUDY TITLE: Ship Shoal: Sand, Shrimp, and Seatrout REPORT TITLE: Blue Crab (Callinectes sapidus) Use of the Ship/Trinity/Tiger Shoal Complex as a Nationally Important Spawning/Hatching/Foraging Ground: Discovery, Evaluation, and Sand Mining Recommendations based on Blue Crab, Shrimp, and Spotted Seatrout Findings CONTRACT NUMBER: 1435-01-04-CA-32806-35161 (M05AZ10660) SPONSORING OCS REGION: Gulf of Mexico APPLICABLE PLANNING AREA: Central FISCAL YEARS OF PROJECT FUNDING: 2004-2009 COMPLETION DATE OF REPORT: December 2009 COSTS: FY 2004-2009, $145,778; CUMMULATIVE PROJECT COSTS: $145,778 PROJECT MANAGER: R. E. Condrey AFFILIATION: Louisiana State University, Department of Oceanography and Coastal Sciences, School of the Coast and Environment ADDRESS: Energy, Coast, and Environment Building, Louisiana State University, Baton Rouge, LA 70803 PRINCIPAL INVESTIGATOR: R.E. Condrey KEY WORDS: Gulf of Mexico, Ship Shoal, Trinity Shoal, Tiger Shoal, Louisiana, sand mining, blue crab, white shrimp, brown shrimp, spotted seatrout, condition factor, fecundity, spawning grounds, coastal restoration BACKGROUND: The Minerals Management Service (MMS) is addressing the recent demand for U.S. continental shelf sand resources for coastal erosion management, a critical challenge to Louisiana’s ecosystems and economies. Louisiana considers barrier island restoration as a promising way to combat wetland loss, with sand mined from Ship Shoal as the most feasible sediment source. Additional sand resources on Trinity and Tiger Shoals are also being considered. Since this area supports major demersal fisheries on white and brown shrimp (Litopenaeus setiferus and Farfantepenaeus aztecus), the present study was conducted as part of a larger effort to understand the sand-mining ecology of the Ship/Trinity/Tiger Shoal Complex (STTSC). -
Dimorphism and the Functional Basis of Claw Strength in Six Brachyuran Crabs
J. Zool., Lond. (2001) 255, 105±119 # 2001 The Zoological Society of London Printed in the United Kingdom Dimorphism and the functional basis of claw strength in six brachyuran crabs Steve C. Schenk1 and Peter C. Wainwright2 1 Department of Biological Science, Florida State University, Tallahassee, Florida 32306, U.S.A. 2 Section of Evolution and Ecology, University of California, Davis, CA 95616, U.S.A. (Accepted 7 November 2000) Abstract By examining the morphological basis of force generation in the chelae (claws) of both molluscivorous and non-molluscivorous crabs, it is possible to understand better the difference between general crab claw design and the morphology associated with durophagy. This comparative study investigates the mor- phology underlying claw force production and intraspeci®c claw dimorphism in six brachyuran crabs: Callinectes sapidus (Portunidae), Libinia emarginata (Majidae), Ocypode quadrata (Ocypodidae), Menippe mercenaria (Xanthidae), Panopeus herbstii (Xanthidae), and P. obesus (Xanthidae). The crushers of the three molluscivorous xanthids consistently proved to be morphologically `strong,' having largest mechan- ical advantages (MAs), mean angles of pinnation (MAPs), and physiological cross-sectional areas (PCSAs). However, some patterns of variation (e.g. low MA in C. sapidus, indistinguishable force generation potential in the xanthids) suggested that a quantitative assessment of occlusion and dentition is needed to understand fully the relationship between force generation and diet. Interspeci®c differences in force generation potential seemed mainly to be a function of differences in chela closer muscle cross- sectional area, due to a sixfold variation in apodeme area. Intraspeci®c dimorphism was generally de®ned by tall crushers with long in-levers, though O. -
The Mitochondrial Genome and Transcriptome of the Basal
View metadata, citation and similar papers at core.ac.uk brought to you by CORE GBEprovided by PubMed Central The Mitochondrial Genome and Transcriptome of the Basal Dinoflagellate Hematodinium sp.: Character Evolution within the Highly Derived Mitochondrial Genomes of Dinoflagellates C. J. Jackson, S. G. Gornik, and R. F. Waller* School of Botany, University of Melbourne, Australia *Corresponding author: E-mail: [email protected]. Accepted: 12 November 2011 Abstract The sister phyla dinoflagellates and apicomplexans inherited a drastically reduced mitochondrial genome (mitochondrial DNA, mtDNA) containing only three protein-coding (cob, cox1, and cox3) genes and two ribosomal RNA (rRNA) genes. In apicomplexans, single copies of these genes are encoded on the smallest known mtDNA chromosome (6 kb). In dinoflagellates, however, the genome has undergone further substantial modifications, including massive genome amplification and recombination resulting in multiple copies of each gene and gene fragments linked in numerous combinations. Furthermore, protein-encoding genes have lost standard stop codons, trans-splicing of messenger RNAs (mRNAs) is required to generate complete cox3 transcripts, and extensive RNA editing recodes most genes. From taxa investigated to date, it is unclear when many of these unusual dinoflagellate mtDNA characters evolved. To address this question, we investigated the mitochondrial genome and transcriptome character states of the deep branching dinoflagellate Hematodinium sp. Genomic data show that like later-branching dinoflagellates Hematodinium sp. also contains an inflated, heavily recombined genome of multicopy genes and gene fragments. Although stop codons are also lacking for cox1 and cob, cox3 still encodes a conventional stop codon. Extensive editing of mRNAs also occurs in Hematodinium sp. -
Chinese Mitten Crab (Eriocheir Sinensis)
www.nonnativespecies.org For definitive identification, contact: [email protected] Chinese mitten crab (Eriocheir sinensis) Synonyms: - big sluiceway crab, Chinese freshwater edible crab, Chinese mittenhanded crab, Chinese mit- ten-handed crab, Chinese river crab, crabe chi- nois, mitten crab, river crab, Shanghai crab, villus crab - Eriocheir japonicas, E. leptognathus, E. rectus Consignments likely to come from: unknown Use: may be used for human consumption Identification difficulty: easy Identification information: - the only freshwater crab present in Britain - body (carapace) olive-green-brown, up to 8cm wide - pincers covered in a mat of fine hair resembling mittens - legs long and hairy Key ID Features Pincers covered in a mat Body (carapace) olive- of fine hair, giving the green-brown, up to 8 cm appearance of mittens wide Up to 8 cm Legs long and hairy Male and female Chinese mitten crabs are similar in appearance except their undersides Underside Underside of female of male Similar species Three other crab species are commonly imported to Britain but these are unlikely to be confused with Chinese mitten crab. Eriocheir sinensis Metacarcinus magister (Chinese mitten crab) (Dungeness crab) for comparison Body up tp to 8cm Legs long across and hairy Body up tp to 25cm across, usually under 20cm Body beige to light brown in Body (carapace) olive- Pincers covered in a mat colour with blue edges green-brown of fine hair, giving the appearance of mittens Callinectes sapidus Portunus pelagicus (Atlantic blue crab) (blue swimmer crab) Legs blue, body greyish / Body up tp to 27cm Vary in colour from dark Body up tp to 21cm greenish brown across brown, blue and purple across Pair of long, pointed Paddle shaped rear legs spines at lateral edges Paddle shaped rear legs of body Photos from: Almandine, Children’s Museum of Indianapolis, Dan Boone—United States Fish and Wildlife Service (all via Wikimedia Commons), & APHA . -
A New Pathogenic Virus in the Caribbean Spiny Lobster Panulirus Argus from the Florida Keys
DISEASES OF AQUATIC ORGANISMS Vol. 59: 109–118, 2004 Published May 5 Dis Aquat Org A new pathogenic virus in the Caribbean spiny lobster Panulirus argus from the Florida Keys Jeffrey D. Shields1,*, Donald C. Behringer Jr2 1Virginia Institute of Marine Science, The College of William & Mary, Gloucester Point, Virginia 23062, USA 2Department of Biological Sciences, Old Dominion University, Norfolk, Virginia 23529, USA ABSTRACT: A pathogenic virus was diagnosed from juvenile Caribbean spiny lobsters Panulirus argus from the Florida Keys. Moribund lobsters had characteristically milky hemolymph that did not clot. Altered hyalinocytes and semigranulocytes, but not granulocytes, were observed with light microscopy. Infected hemocytes had emarginated, condensed chromatin, hypertrophied nuclei and faint eosinophilic Cowdry-type-A inclusions. In some cases, infected cells were observed in soft con- nective tissues. With electron microscopy, unenveloped, nonoccluded, icosahedral virions (182 ± 9 nm SD) were diffusely spread around the inner periphery of the nuclear envelope. Virions also occurred in loose aggregates in the cytoplasm or were free in the hemolymph. Assembly of the nucleocapsid occurred entirely within the nucleus of the infected cells. Within the virogenic stroma, blunt rod-like structures or whorls of electron-dense granular material were apparently associated with viral assembly. The prevalence of overt infections, defined as lethargic animals with milky hemolymph, ranged from 6 to 8% with certain foci reaching prevalences of 37%. The disease was transmissible to uninfected lobsters using inoculations of raw hemolymph from infected animals. Inoculated animals became moribund 5 to 7 d before dying and they began dying after 30 to 80 d post-exposure. -
Growth Rate Variability and Lipofuscin Accumulation Rates in the Blue Crab Callinectes Sapidus
MARINE ECOLOGY PROGRESS SERIES Vol. 224: 197–205, 2001 Published December 19 Mar Ecol Prog Ser Growth rate variability and lipofuscin accumulation rates in the blue crab Callinectes sapidus Se-Jong Ju, David H. Secor, H. Rodger Harvey* Chesapeake Biological Laboratory, University of Maryland Center for Environmental Science, PO Box 38, Solomons, Maryland 20688, USA ABSTRACT: To better understand growth and age-pigment (lipofuscin) accumulation rates of the blue crab Callinectes sapidus under natural conditions, juveniles (33 to 94 mm carapace width) were reared in outdoor ponds for over 1 yr. Growth rates, measured by carapace width, during summer and fall exceeded all those reported in the literature; the initial carapace width of 59 ± 14 mm (mean ± SD) increased to 164 ± 15 mm within a 3 mo period. No growth occurred during winter months (Novem- ber to April) at low water temperatures. Growth rates of crabs in ponds were substantially higher (von Bertalanffy growth parameter K = 1.09) than those of crabs held in laboratory environments, and than rate estimates for natural populations of mid-Atlantic blue crabs. Model comparisons indicated that seasonalized von Bertalanffy growth models (r2 > 0.9) provide a better fit than the non-seasonalized model (r2 = 0.74) for pond-reared crabs and, by implication, are more appropriate for field popula- tions. Despite growth rates that varied strongly with season, lipofuscin (normalized to protein con- centration) accumulation rate was nearly constant throughout the year. Although the lipofuscin level in pond-reared crabs was significantly correlated with size (carapace width), it was more closely cor- related with chronological age. -
<I>Hematodinium Perezi</I>
Old Dominion University ODU Digital Commons Biological Sciences Faculty Publications Biological Sciences 2019 Parasitic Dinoflagellate Hematodinium perezi Prevalence in Larval and Juvenile Blue Crabs Callinectes sapidus from Coastal Bays of Virginia H. J. Small J. P. Huchin-Mian K. S. Reece K. M. Pagenkopp Lohan Mark J. Butler IV Old Dominion University, [email protected] See next page for additional authors Follow this and additional works at: https://digitalcommons.odu.edu/biology_fac_pubs Part of the Biology Commons, Marine Biology Commons, and the Parasitology Commons Original Publication Citation Small, H. J., Huchin-Mian, J. P., Reece, K. S., Lohan, K. M. P., Butler, M. J., & Shields, J. D. (2019). Parasitic dinoflagellate Hematodinium perezi prevalence in larval and juvenile blue crabs Callinectes sapidus from coastal bays of Virginia. Diseases of Aquatic Organisms, 134(3), 215-222. doi:10.3354/dao03371 This Article is brought to you for free and open access by the Biological Sciences at ODU Digital Commons. It has been accepted for inclusion in Biological Sciences Faculty Publications by an authorized administrator of ODU Digital Commons. For more information, please contact [email protected]. Authors H. J. Small, J. P. Huchin-Mian, K. S. Reece, K. M. Pagenkopp Lohan, Mark J. Butler IV, and J. D. Shields This article is available at ODU Digital Commons: https://digitalcommons.odu.edu/biology_fac_pubs/391 Vol. 134: 215–222, 2019 DISEASES OF AQUATIC ORGANISMS Published online June 6 https://doi.org/10.3354/dao03371 Dis Aquat Org OPENPEN ACCESSCCESS Parasitic dinoflagellate Hematodinium perezi prevalence in larval and juvenile blue crabs Callinectes sapidus from coastal bays of Virginia H. -
ABSTRACT Dinoflagellate Parasites in the Hematodinium Genus Have
ABSTRACT Title of Document: DEVELOPMENT, VALIDATION AND APPLICATION OF A QUANTITATIVE POLYMERASE CHAIN REACTION ASSAY TO ASSESS ENVIRONMENTAL SAMPLES FOR HEMATODINIUM PEREZI PREVALENCE Ammar W. Hanif, MS, 2012 Directed By: Assistant Professor Eric J. Schott, Institute of Marine and Environmental Technology, University of Maryland Center of Environmental Science Associate Professor Rosemary Jagus, Co- advisor, Institute of Marine and Environmental Technology, University of Maryland Center of Environmental Science Dinoflagellate parasites in the Hematodinium genus have emerged as important pathogens of economically important crustaceans worldwide, causing significant economic losses to fisheries and aquaculture. An understanding of the routes of infection in blue crab (Callinectes sapidus) populations would be facilitated by an improved knowledge of environmental reservoirs. A previously used PCR assay, based on small subunit rRNA sequences, lacked the specificity needed for Hematodinium perezi detection of environmental samples. Therefore a quantitative PCR assay based on the internal transcribed spacer 2 (ITS2) region of H. perezi rRNA genes was developed, validated, and applied to examine temporal and spatial incidences of environmental reservoirs in Delmarva coastal bays. H. perezi was detected in sediment and water in several Delmarva coastal bays, as well as the host, C. sapidus. Results suggest the existence of localized sediment reservoirs in areas where hydrological and geophysical features allow for the formation of cell deposits. DEVELOPMENT, VALIDATION, AND APPLICATION OF A QUANTITATIVE POLYMERASE CHAIN REACTION ASSAY TO ASSESS HEMATODINIUM PEREZI PREVALENCE IN ENVIRONMENTAL SAMPLES By Ammar W. Hanif Thesis submitted to the Faculty of the Graduate School of the University of Maryland College Park, in partial fulfillment of the requirements for the degree of Master of Science 2012 Advisory committee: Professor Eric J. -
Blue Crab. U.S
~C' k""~-- - -- - S..- - _ __ - -. .54.5 .tS-'--------- - - - -tt Coastal Ecology Group Fish and Wildlife Service . Waterways Experiment Station U.S. Department of the Interior U.S. Army Corps of Engineers c-ýz N7cw jo r ýs Biological Report 82(11.100) TR EL-82-4 March 1989 Species Profiles: Life Histories and Environmental Requirements of Coastal Fishes and Invertebrates (Mid-Atlantic) BLUE CRAB by Jennifer Hill, Dean L. Fowler, and Michael J. Van Den Avyle Georgia Cooperative Fish and Wildlife Research Unit School of Forest Resources University of Georgia Athens, GA 30602 Project Officer David Moran U.S. Fish and Wildlife Service National Wetlands Research Center 1010 Gause Boulevard Slidell, LA 70458 Performed for U.S. Army Corps of Engineers Coastal Ecology Group Waterways Experiment Station Vicksburg, MS 39180 and U.S. Department of the Interior Fish and Wildlife Service Research and Development National Wetlands Research Center Washington, DC 20240 DISCLAIMER The mention of trade nanes in this report does not constitute endorsement nor recommendation for use by the U.S. Fish and Wildlife Service or Federal Government. This series may be referenced as follows: U.S. Fish and Wildlife Service. 1983-19 Species profiles: life histories and environmental requirements of coastail fishes and invertebrates. U.S. Fish Wildl. Serv. Biol. Rep. 82(11). U.S. Army Corps of Engineers, TR EL-82-4. This profile may be cited as follows: Hill, J., D.L. Fowler, and M.J. Van Den Avyle. 1989. Species profiles: life histories and environmental requirements of coastal fishes and invertebrates (Mid-Atlantic)--Blue crab.