DOCSLIB.ORG

Alterations in Ptch1 Cis-Regulation Underlie Loss of Antero-Posterior

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Alterations in Ptch1 Cis-Regulation Underlie Loss of Antero-Posterior Alterations in Ptch1 Cis-Regulation underlie loss of antero-posterior identity and digit reductions in bovine limbs Rolf Zeller, Amandine Duchesne, Sepziale Dario, Guillaume Andrey, Erkan Uenal, Christian Basel, Benoit Robert, Carol Wicking, Denis Duboule, Javier Lopez-Rios To cite this version: Rolf Zeller, Amandine Duchesne, Sepziale Dario, Guillaume Andrey, Erkan Uenal, et al.. Alterations in Ptch1 Cis-Regulation underlie loss of antero-posterior identity and digit reductions in bovine limbs. 16. International Society of Developmental Biologists Congress, Jun 2013, Cancun, Mexico. hal- 01193898 HAL Id: hal-01193898 https://hal.archives-ouvertes.fr/hal-01193898 Submitted on 3 Jun 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. International Society of Developmental Biologists 17th International Congress of Developmental Biology Cancun, Mexico June 16 - 20, 2013 72nd Annual Meeting of the Society for Developmental Biology VII Latin American Society of Developmental Biology Meeting XI Congreso de la Sociedad Mexicana de Biologia del Desarrollo ABSTRACTS 1 Program/Abstract # 1 Mechanical force controls chemical signals in creating plant pattern Elliot Meyerowitz (Caltech, USA) One pattern generated by the stem cells at the tip of each plant shoot – the shoot apical meristem – of flowering plants has held a fascination for generations of biologists and mathematicians. This is the phyllotactic pattern, the pattern of leaves and flowers around the stem. The most common such pattern is the spiral phyllotactic pattern, which creates the highly recognizable organization of compound fruits such as pineapples, of flowers like roses, and of inflorescences such as sunflowers. The model plant Arabidopsis thaliana also has a spiral phyllotaxis, and we have used genetic, genomic, and cell biological approaches to learn in detail how the cells of the meristem collaborate to generate this pattern. The major chemical signal is auxin, which has a specific transport system, in which a family of plasma membrane proteins directs the efflux of the hormone from cells. The efflux proteins are not uniformly distributed, thereby causing efflux directionally, leading to a net flow of auxin in complex patterns across the surface of the meristem. Auxin not only induces new primordia of leaves and flowers, but also changes the physical properties of the cell wall. These physical changes alter the stress pattern in the meristem surface, which in turn regulates the position of the auxin efflux carrier in anisotropically stressed cells. The feedback between auxin concentration and physical stress creates the dynamic auxin patterns that cause successive auxin peaks at positions approximately 130-140 degrees around the stem, creating the spiral (and other patterns of) phyllotaxis. The stress pattern in the meristem also regulates the microtubule cytoskeleton of meristematic cells, and consequently it may also dictate the plane of cell division. The stress pattern may also determine directions of cellulose synthesis in the cell wall, and thus the subsequent direction of cellular growth, and the anisotropy of cells – leading to changes in auxin flow, and, consequently, feedbacks on the stress pattern. Program/Abstract # 2 Unusual patterns of Hox cluster evolution Peter Holland, Ferdinand Marletaz, Laura Ferguson, Jordi Papas, (Oxford, UK), Fei Xu (Chinese Academy of Science, China), Willie Taylor (NIMR, UK), Pete Olson (Nat Hist Museum London, UK) In the 1980s, the discovery of Hox gene clusters in very different animals paved the way for an integrated science in which principles and processes of embryonic development could be compared between widely divergent evolutionary lineages. A picture has emerged of a conserved Hox gene cluster pattering the ancient head-to-tail axis across all bilaterian animals. Yet there are modifications, and these differences between species may be very helpful in our attempts to link genotype evolution to phenotype evolution. Using examples from on-going research, I will discuss intriguing examples of Hox cluster breakage, Hox cluster expansion and Hox cluster shrinkage. Program/Abstract # 3 Regulation of gene expression by RNA polymerase II promoter pausing during mouse embryonic development Megan Jane Wilson (Univ. of Otago, New Zealand) Proximal-promoter pausing by RNA polymerase II (RNA-polII) is a key rate-limiting step in transcription initiation. Recent genome- wide studies using chromatin-immunoprecipitation to detect stalled RNA-polII have shown that promoter-pausing occurs for a number of genes, particularly developmental control genes. This stalling is believed to be a mechanism of gene regulation, causing RNA-polII to be paused near a promoter region, ready to respond to environmental or developmental cues. Two transcription elongation factors DSIF and NELF control promoter stalling by RNA-polII. Our laboratory studies sex-specific differentiation of developing mouse embryo tissues and we have utilized this system to study RNA polII stalling and its effect on gene expression over key developmental stages. Using ChIP-seq with antibodies against RNApolII, we identified many promoters that have RNA-polII stalled in a sex-specific manor in both gonad and head tissue at 13.5 dpc. This corresponded to differences in gene expression between the sexes for the associated gene transcript (as assayed by qPCR). For some transcripts, RNA polII stalling marked them for future activation at a later time point in development. In other cases, paused RNA polII was associated with genes that were becoming down regulated. This data also reveals that promoter pausing can occur differently between sexes during development. We also have preliminary evidence that indicates that components of NELF and DSIF complexes are expressed differently between the sexes during embryogenesis. Together our data suggests that some genes are being poised to respond to a signal and then strongly upregulated, whereas transcription at other genes is stalled but not activated, perhaps in the absence of an appropriate signal. Program/Abstract # 4 Withdrawn Program/Abstract # 5 Characterization of human developmental enhancers: from whole genomes to single SNPs Alvaro Rada Iglesias (Univ. of Cologne, Germany) Distal regulatory elements, such as enhancers, play a preponderant role in the establishment of cell-type and developmental-stage specific gene expression profiles. However, these elements are difficult to identify, since they lack strong defining features and show limited sequence conservation. In the first part of my talk, I will summarize my postdoctoral work, in which epigenomic approaches were used to characterize the enhancer repertoire of pluripotent (i.e. human embryonic stem cells (hESC)) and multipotent (i.e. human 2 neural crest cells (hNCC)) human embryonic cell populations. In hESC, we uncovered a unique chromatin signature that identifies a novel class of enhancers, which are inactive but poised in hESC and that become active upon differentiation in a lineage-specific manner. Similarly, our epigenomic approach allowed us to characterize enhancers in hNCC, a hitherto largely inaccessible and biochemically intractable vertebrate-specific embryonic cell population that contributes to the formation of multiple tissues and organs, such as the peripheral nervous system and most of the facial bones and cartilages. Using the sequence information contained within hNCC enhancers, we uncovered NR2F1 and NR2F2, two orphan nuclear receptors, as novel neural crest and craniofacial regulators. Finally, I will briefly describe how the genomic characterization of human enhancers in relevant cell types might streamline the identification of functional non-coding genetic variants, which can have far-reaching implications in our understanding of the genetic basis of human complex diseases and human morphological evolution. Program/Abstract # 6 Super-resolution imaging of regulatory chromatin dynamics in developing embryos Alistair Boettiger, Xiaowei Zhuang (Harvard, USA) The differentiation of embryonic cells into their appropriate developmental fates is mediated in part by fine scale structural changes to chromatin. Developmental specific transcription factors may shape this chromatin structure through a variety of mechanisms, such as re-positioning of histones (e.g. to restrict or increase access to DNA) or the generation of higher-order looped chromatin structures (e.g. to facilitate looping of distal regulatory sequences to target sites). These fine scale structural changes are mostly too small (10s of nanometers) to be observed with conventional microscopy techniques (limited to several hundred nanometer resolution), and have so far evaded in vivo observation in intact embryonic tissue. We present super-resolution imaging techniques which allow for the detection of changes in chromatin on the scale of tens of nanometers in developing embryos at gene locations of interest. We can detect locus-specific clusters of modified histones and regulatory chromatin proteins,
Load more

Recommended publications
  • Hedgehog Signaling Is Evolutionarily Conserved Cilium-Independent
    Downloaded from genesdev.cshlp.org on August 14, 2009 - Published by Cold Spring Harbor Laboratory Press Cilium-independent regulation of Gli protein function by Sufu in Hedgehog signaling is evolutionarily conserved Miao-Hsueh Chen, Christopher W. Wilson, Ya-Jun Li, et al. Genes Dev. 2009 23: 1910-1928 Access the most recent version at doi:10.1101/gad.1794109 Supplemental http://genesdev.cshlp.org/content/suppl/2009/07/23/23.16.1910.DC1.html Material References This article cites 97 articles, 47 of which can be accessed free at: http://genesdev.cshlp.org/content/23/16/1910.full.html#ref-list-1 Email alerting Receive free email alerts when new articles cite this article - sign up in the box at the service top right corner of the article or click here To subscribe to Genes & Development go to: http://genesdev.cshlp.org/subscriptions Copyright © 2009 by Cold Spring Harbor Laboratory Press Downloaded from genesdev.cshlp.org on August 14, 2009 - Published by Cold Spring Harbor Laboratory Press Cilium-independent regulation of Gli protein function by Sufu in Hedgehog signaling is evolutionarily conserved Miao-Hsueh Chen,1,3 Christopher W. Wilson,1,3 Ya-Jun Li,1 Kelvin King Lo Law,2 Chi-Sheng Lu,1 Rhodora Gacayan,1 Xiaoyun Zhang,2 Chi-chung Hui,2 and Pao-Tien Chuang1,4 1Cardiovascular Research Institute, University of California at San Francisco, San Francisco, California 94158, USA; 2Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, and Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5G 1L7, Canada A central question in Hedgehog (Hh) signaling is how evolutionarily conserved components of the pathway might use the primary cilium in mammals but not fly.
    [Show full text]
  • Discovery of the Critically Endangered Annual Killifish, Austrolebias
    JoTT SHORT COMMUNI C ATION 2(11): 1282-1285 Discovery of the Critically Endangered annual killifishAustrolebias wolterstorffi (Ahl, 1924) (Cyprinodontiformes: Rivulidae) in Lagoa do Peixe National Park, Rio Grande do Sul, southern Brazil L.E.K. Lanés 1 & L. Maltchik 2 1,2 Laboratory of Ecology and Conservation of Aquatic Ecosystems, University of Vale do Rio dos Sinos (UNISINOS). Av. Unisinos 950, Bairro Cristo Rei, CEP 93022-000, São Leopoldo, Rio Grande do Sul, Brazil. Email: 1 [email protected], 2 [email protected] Abstract: This paper documents the discovery of the rare and live in shallow temporary wetlands formed close to rivers, Critically Endangered annual killifish Austrolebias wolterstorffi streams and lagoons during the rainy season, and die in temporary wetland of Lagoa do Peixe National Park, an im- portant conservation unit of southern Brazil protected under the off when the pools dry (Costa 2003, 2006). Their eggs Ramsar Convention and recognized Biosphere Reserve. survive the dry season and hatch after the next rainy season begins. Keywords: Annual fish, Cynolebiasinae, endemic, freshwater, The genus Austrolebias has recently been redefined Neotropics, protected areas, Ramsar sites, restricted-range fish- phylogenetically by Costa (2006), including the species es, threatened species, wetlands. previously referred to the genus Megalebias Costa. Subsequently the genus Austrolebias was divided into subgenera (Costa 2008), according to clades defined in The neotropical aplocheiloid genus Austrolebias Costa (2006). Accordingly the subgenus Megalebias Costa comprises 40 small annual killifishes endemic to which has the same general geographic range as South America, distributed in southern Brazil, southern Austrolebias, comprises five valid species: Austrolebias Bolivia, Paraguay, Uruguay and northeastern Argentina cheradophilus (Vaz-Ferreira, Sierra de Soriano & Scaglia- (Costa 2010).
    [Show full text]
  • Genetic Mutations Underlying Phenotypic Plasticity in Basosquamous Carcinoma Audris Chiang1,2,7, Caroline Z
    See related commentary on pg 2258 ORIGINAL ARTICLE Genetic Mutations Underlying Phenotypic Plasticity in Basosquamous Carcinoma Audris Chiang1,2,7, Caroline Z. Tan1,7, Franc¸ois Kuonen1, Luqman M. Hodgkinson1, Felicia Chiang3, Raymond J. Cho4, Andrew P. South5,JeanY.Tang1, Anne Lynn S. Chang1, Kerri E. Rieger1,6, Anthony E. Oro1 and Kavita Y. Sarin1 Basosquamous carcinoma (BSC) is an aggressive skin neoplasm with the features of both basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). While genetic drivers of BCC and SCC development have been extensively characterized, BSC has not been well studied, and it remains unclear whether these tumors orig- inally derive from BCC or SCC. In addition, it is unknown which molecular pathways mediate the reprog- ramming of tumor keratinocytes toward basaloid or squamatized phenotypes. We sought to characterize the genomic alterations underlying sporadic BSC to elucidate the derivation of these mixed tumors. We identifed frequent Hedgehog (Hh) pathway mutations in BSCs, implicating Hh deregulation as the primary driving event in BSC. Principal component analysis of BCC and SCC driver genes further demonstrate the genetic similarity between BCC and BSC. In addition, 45% of the BSCs harbor recurrent mutations in the SWI/SNF complex gene, ARID1A, and evolutionary analysis revealed that ARID1A mutations occur after PTCH1 but before SCC driver mutations, indicating that ARID1A mutations may bestow plasticity enabling squamatization. Finally, we demonstrate mitogen-activated protein kinase pathway activation and the loss of Hh signaling associated with the squamatization of BSCs. Overall, these results support the genetic derivation of BSCs from BCCs and highlight potential factors involved in modulating tumor reprogramming between basaloid and squamatized phenotypes.
    [Show full text]
  • Deterministic Shifts in Molecular Evolution Correlate with Convergence to Annualism in Killifishes
    bioRxiv preprint doi: https://doi.org/10.1101/2021.08.09.455723; this version posted August 10, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Deterministic shifts in molecular evolution correlate with convergence to annualism in killifishes Andrew W. Thompson1,2, Amanda C. Black3, Yu Huang4,5,6 Qiong Shi4,5 Andrew I. Furness7, Ingo, Braasch1,2, Federico G. Hoffmann3, and Guillermo Ortí6 1Department of Integrative Biology, Michigan State University, East Lansing, Michigan 48823, USA. 2Ecology, Evolution & Behavior Program, Michigan State University, East Lansing, MI, USA. 3Department of Biochemistry, Molecular Biology, Entomology, & Plant Pathology, Mississippi State University, Starkville, MS 39759, USA. 4Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Marine, Shenzhen 518083, China. 5BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China. 6Department of Biological Sciences, The George Washington University, Washington, DC 20052, USA. 7Department of Biological and Marine Sciences, University of Hull, UK. Corresponding author: Andrew W. Thompson, [email protected] bioRxiv preprint doi: https://doi.org/10.1101/2021.08.09.455723; this version posted August 10, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract: The repeated evolution of novel life histories correlating with ecological variables offer opportunities to test scenarios of convergence and determinism in genetic, developmental, and metabolic features. Here we leverage the diversity of aplocheiloid killifishes, a clade of teleost fishes that contains over 750 species on three continents.
    [Show full text]
  • Recent Advances in Signaling Pathways Comprehension As Carcinogenesis Triggers in Basal Cell Carcinoma
    Journal of Clinical Medicine Review Recent Advances in Signaling Pathways Comprehension as Carcinogenesis Triggers in Basal Cell Carcinoma Mircea Tampa 1,2, Simona Roxana Georgescu 1,2,*, Cristina Iulia Mitran 3, Madalina Irina Mitran 3, Clara Matei 1, Cristian Scheau 4 , Carolina Constantin 5,6 and Monica Neagu 5,6,7 1 Department of Dermatology, “Carol Davila” University of Medicine and Pharmacy, 050474 Bucharest, Romania; [email protected] (M.T.); [email protected] (C.M.) 2 Department of Dermatology, “Victor Babes” Clinical Hospital for Infectious Diseases, 030303 Bucharest, Romania 3 Department of Microbiology, “Carol Davila” University of Medicine and Pharmacy, 050474 Bucharest, Romania; [email protected] (C.I.M.); [email protected] (M.I.M.) 4 Department of Physiology, “Carol Davila” University of Medicine and Pharmacy, 050474 Bucharest, Romania; [email protected] 5 Immunology Department, “Victor Babes” National Institute of Pathology, 050096 Bucharest, Romania; [email protected] (C.C.); [email protected] (M.N.) 6 Colentina Clinical Hospital, 020125 Bucharest, Romania 7 Faculty of Biology, University of Bucharest, 76201 Bucharest, Romania * Correspondence: [email protected] Received: 18 July 2020; Accepted: 16 September 2020; Published: 18 September 2020 Abstract: Basal cell carcinoma (BCC) is the most common malignant skin tumor. BCC displays a different behavior compared with other neoplasms, has a slow evolution, and metastasizes very rarely, but sometimes it causes an important local destruction. Chronic ultraviolet exposure along with genetic factors are the most important risk factors involved in BCC development. Mutations in the PTCH1 gene are associated with Gorlin syndrome, an autosomal dominant disorder characterized by the occurrence of multiple BCCs, but are also the most frequent mutations observed in sporadic BCCs.
    [Show full text]
  • Loss of the PTCH1 Tumor Suppressor Defines a New
    Banerjee et al. J Transl Med (2019) 17:246 https://doi.org/10.1186/s12967-019-1995-z Journal of Translational Medicine RESEARCH Open Access Loss of the PTCH1 tumor suppressor defnes a new subset of plexiform fbromyxoma Sudeep Banerjee1,2, Christopher L. Corless3, Markku M. Miettinen4, Sangkyu Noh1, Rowan Ustoy1, Jessica L. Davis3, Chih‑Min Tang1, Mayra Yebra1, Adam M. Burgoyne5 and Jason K. Sicklick1* Abstract Background: Plexiform fbromyxoma (PF) is a rare gastric tumor often confused with gastrointestinal stromal tumor. These so‑called “benign” tumors often present with upper GI bleeding and gastric outlet obstruction. It was recently demonstrated that approximately one‑third of PF have activation of the GLI1 oncogene, a transcription factor in the hedgehog (Hh) pathway, via a MALAT1‑GLI1 fusion protein or GLI1 up‑regulation. Despite this discovery, the biology of most PFs remains unknown. Methods: Next generation sequencing (NGS) was performed on formalin‑fxed parafn‑embedded (FFPE) samples of PF specimens collected from three institutions (UCSD, NCI and OHSU). Fresh frozen tissue from one tumor was utilized for in vitro assays, including quantitative RT‑PCR and cell viability assays following drug treatment. Results: Eight patients with PF were identifed and 5 patients’ tumors were analyzed by NGS. An index case had a mono‑allelic PTCH1 deletion of exons 15–24 and a second case, identifed in a validation cohort, also had a PTCH1 gene loss associated with a suspected long‑range chromosome 9 deletion. Building on the role of Hh signaling in PF, PTCH1, a tumor suppressor protein, functions upstream of GLI1. Loss of PTCH1 induces GLI1 activation and down‑ stream gene transcription.
    [Show full text]
  • Understanding the Application: NGS Panel Testing for Hereditary Cancer Syndromes and Cancer Targeted Therapy
    Understanding the Application: NGS Panel Testing for Hereditary Cancer Syndromes and Cancer Targeted Therapy Felicitas L. Lacbawan, MD, FCAP, FACMG Medical Director, Genetics Quest Diagnostics Nichols Institute, San Juan Capistrano, CA USA February 9, 2016 [email protected] http://www.cancer.org/acs/groups/content/@editorial/documents/document/acspc-044552.pdf 2 http://www.nature.com/onc/journal/v23/n38/full/1207714a.html Hereditary Cancers About 5% to 10% of all cancers Inheriting a gene mutation or pathogenic variant does not necessarily mean that a person will develop cancer, but it increases his/her risk Most common hereditary cancers are: Breast cancer Ovarian cancer Colorectal cancer Prostate cancer Understanding if cancer is due to an inherited pathogenic variant/mutation can help clarify future risks of developing cancer and help determine options for cancer screening and prevention, possibly therapy 5 Lifetime Cancer Risks for Common Cancers Cancer Risk (%) Cancer Type http://seer.cancer.gov/ Lifetime Risks: Breast Cancer “Red Flags” for Inherited Susceptibility to Cancer Cancer in 2 or more closely related relatives Multiple generations affected Early age at diagnosis Multiple primary tumors Bilateral or rare cancers Constellation of tumors consistent with a specific cancer syndrome Certain ethnic backgrounds (e.g. Ashkenazi Jewish ancestry) 8 Assessing Patient’s Family History Ovarian, 52 Breast, 42 Breast, 45 9 Cost per Genome Decreasing Dramatically Diagnostic Applications of Sequencing
    [Show full text]
  • The Neotropical Genus Austrolebias: an Emerging Model of Annual Killifishes Nibia Berois1, Maria J
    lopmen ve ta e l B D io & l l o l g e y C Cell & Developmental Biology Berois, et al., Cell Dev Biol 2014, 3:2 ISSN: 2168-9296 DOI: 10.4172/2168-9296.1000136 Review Article Open Access The Neotropical Genus Austrolebias: An Emerging Model of Annual Killifishes Nibia Berois1, Maria J. Arezo1 and Rafael O. de Sá2* 1Departamento de Biologia Celular y Molecular, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay 2Department of Biology, University of Richmond, Richmond, Virginia, USA *Corresponding author: Rafael O. de Sá, Department of Biology, University of Richmond, Richmond, Virginia, USA, Tel: 804-2898542; Fax: 804-289-8233; E-mail: [email protected] Rec date: Apr 17, 2014; Acc date: May 24, 2014; Pub date: May 27, 2014 Copyright: © 2014 Rafael O. de Sá, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract Annual fishes are found in both Africa and South America occupying ephemeral ponds that dried seasonally. Neotropical annual fishes are members of the family Rivulidae that consist of both annual and non-annual fishes. Annual species are characterized by a prolonged embryonic development and a relatively short adult life. Males and females show striking sexual dimorphisms, complex courtship, and mating behaviors. The prolonged embryonic stage has several traits including embryos that are resistant to desiccation and undergo up to three reversible developmental arrests until hatching. These unique developmental adaptations are closely related to the annual fish life cycle and are the key to the survival of the species.
    [Show full text]
  • Discovery of Endangered Annual Killifish Austrolebias
    Neotropical Ichthyology, 12(1): 117-124, 2014 Copyright © 2014 Sociedade Brasileira de Ictiologia Discovery of endangered annual killifish Austrolebias cheradophilus (Aplocheiloidei: Rivulidae) in Brazil, with comments on habitat, population structure and conservation status Luis Esteban Krause Lanés1,2, Ândrio Cardozo Gonçalves1,3 and Matheus Vieira Volcan1,3 Austrolebias genus comprises about 40 small annual killifishes endemic to South America and their highest diversity occurs in southern Brazil and Uruguay, especially in drainages of Patos-Mirim system. Austrolebias species are severely threatened with extinction because their life cycle and restricted ranges. Low dispersal ability and the extensive loss and fragmentation of freshwater wetlands contribute to this threat. Accurate information on the geographic distribution and ecology of the species, vital to plan conservation and management strategies, are scarce. In order to provide basic knowledge for annual fish conservation this paper reports the presence of Austrolebias cheradophilus and present data about its population structure (CPUA, size, sex ratio, length-weight relationships and condition factor) and conservation status in southern Brazil. The estimated CPUA of populations was 0.86 fish/m2. Standard length (SL) of males ranged between 32.14 and 49.17 mm and for females between 25.11 and 41.6 mm. There were no differences in SL between the sexes (t-test = - 1.678; P = 0.105), and Chi-squared test demonstrated marginal differences in proportions of sexes (2.25:1; χ2 = 3.846; P = 0.07). Allometric coefficient of theLWR was slightly hyperallometric (b = 3.08) and K of the specimens ranged from 1.84 to 2.42 (mean ± S.E.
    [Show full text]
  • Austrolebias Bagual, a New Species of Annual Fish (Cyprinodontiformes: Rivulidae) from Southern Brazil
    AQUA20(4)-LAYOUT.qxp_AQUA 16/10/14 09:50 Pagina 161 aqua, International Journal of Ichthyology Austrolebias bagual, a new species of annual fish (Cyprinodontiformes: Rivulidae) from southern Brazil Matheus Vieira Volcan 1,2, Luis Esteban Krause Lanés1,3 and Ândrio Cardozo Gonçalves1 1) Instituto Pró-Pampa (IPPampa), Laboratório de Ictiologia. Pelotas, Rio Grande do Sul, Brasil. E-mail: [email protected] 2) Universidade Federal de Santa Maria (UFSM). Programa de Pós Graduação em Biodiversidade Animal. Santa Maria, Rio Grande do Sul, Brasil. 3) Universidade do Vale do Rio dos Sinos (UNISINOS), Programa de Pós Graduação em Biologia: Diversidade e Manejo da Vida Silvestre, Laboratório de Ecologia e Conservação de Ecossistemas Aquáticos, São Leopoldo, Rio Grande do Sul, Brasil. Received: 22 September 2014 – Accepted: 10 July 2014 Abstract adloffi por la altura inferior del cuerpo y cabeza (excepto en Austrolebias bagual, a new species of annual fish closely re- A. reicherti), más corta longitud de la base de la aleta anal y lated to the A. adloffi species group, is described from spec- tamaño de la aleta caudal en los machos, y la altura de la imens collected from temporary ponds located in the mid- cabeza más baja (excepto en A. reicherti) y el tamaño de la dle course of the Rio Camaquã, Laguna dos Patos system at aleta anal en las hembras. Por otra parte, las hembras de A. the municipality of Encruzilhada do Sul, Rio Grande do bagual no presentan un par de manchas negras dispuestas Sul, Brazil. The new species is distinguished from other ver ti calmente en la parte posterior del pedúnculo caudal, Austrolebias species by the unique male pigmentation pat- típico de la mayoría de las especies del grupo A.
    [Show full text]
  • Biblioteca JORGE D
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by SEDICI - Repositorio de la UNLP Reprinted from Herpetoi.ogica Vol. 24, June 28, 1968, No. 2 pp. 141-146 Made in United States of America biblioteca JORGE D. WILLIAMS NOTES ON THE TADPOLES AND BREEDING ECOLOGY OF LEPIDOB ATRAC HUS (AMPHIBIA: CERATOPHRYIDAE) J. M. Cei BIBLIOTECA JORGE D. WILLIAMS NOTES ON THE TADPOLES AND BREEDING ECOLOGY OF LEPIDOBATRACHUS (AMPHIBIA: CERATOPHRYIDAE) J. M. Cei Lepidobatrachus is a characteristic Chacoan genus of the Ceratophryidae, which we consider to be an independent Neotrop­ ical phyletic line of leptodactylids. Its earliest known representative is the Miocene Wawelia from Patagonia (Casamiquela, 1963). Since the discovery of the genus by Budgett (1899), Lepidoba­ trachus has received relatively little comment. Vellard (1948) re­ described the type-species, and the generic status has been con­ firmed by Cei (1958), Reig and Cei (1963), and Barrio (1967) utilizing various lines of investigation. The latter author proposes recognizing three species: L. laevis Budgett, L. asper Budgett (L. salinicola Reig and Cei is a synonym), and L. llanensis Reig and Cei, whose distributions are largely allopatric but in part sym­ patric (Fig. 1). Except for Parker’s (1931) brief description and figures of the tadpole of Lepidobatrachus asper (= either asper or laevis by current concepts), the larvae of the genus have not been described. The tadpoles of L. asper and L. llanensis are described and figured in this paper. These species occur in the shrub-covered flats of the Argentine Central and Western Chacoan provinces.
    [Show full text]
  • How a Shift in Phenology Affects Critical Life‐History Traits in Annual Fishes
    Received: 22 February 2019 | Revised: 17 June 2019 | Accepted: 19 June 2019 DOI: 10.1111/fwb.13376 ORIGINAL ARTICLE Changing patterns of growth in a changing planet: How a shift in phenology affects critical life‐history traits in annual fishes Daniel García1 | Carl Smith2,3,4,5 | Emanuel Machín1 | Marcelo Loureiro1 | Martin Reichard2 1Departamento de Ecología y Evolución, Facultad de Ciencias, Instituto Abstract de Biología, Universidad de la República, 1. Changes to the timing of key life‐cycle events can alter selection on life‐history Montevideo, Uruguay traits and have the potential to drive a cascade of effects at the community level. 2The Czech Academy of Sciences, Institute of Vertebrate Biology, Brno, Czech Republic We investigated how the growth rate and sexual maturation of two annual killifish 3Department of Ecology and Vertebrate species (Austrolebias bellottii and Austrolebias nigripinnis) were altered by a change Zoology, University of Łódź, Łódź, Poland in the pattern of precipitation. These are annual species, endemic to ephemeral 4School of Animal, Rural and Environmental Sciences, Nottingham Trent University, pools, persist in desiccated sediment as partly developed embryos. Southwell, Nottinghamshire, UK 2. We sampled 18 pools supporting populations of both species in a Pampa region 5School of Biology and Bell‐Pettigrew adjacent to the Rio Negro in western Uruguay throughout the austral winter of Museum of Natural History, University of St Andrews, St Andrews, UK 2015. Fish growth and maturation were monitored from pool inundation to habi‐ tat desiccation, along with environmental variables. Correspondence Martin Reichard, The Czech Academy of 3. The region experienced an unprecedented mid‐winter desiccation of pools that Sciences, Institute of Vertebrate Biology, usually contain water from autumn to late spring, typically only desiccating in Květná 8, 603 65, Brno, Czech Republic.
    [Show full text]